Inna Khozin-Goldberg

Inna Khozin-Goldberg
Ben-Gurion University of the Negev | bgu · Jacob Blaustein Institutes for Desert Research

Professor

About

179
Publications
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Introduction
Inna Khozin-Goldberg currently works at the Jacob Blaustein Institutes for Desert Research , Ben-Gurion University of the Negev. Inna does research in Microalgal Biotechnology, Lipid Biochemistry, Molecular Biology and Phycology.
Additional affiliations
November 1994 - present
Ben-Gurion University of the Negev

Publications

Publications (179)
Article
Full-text available
Arachidonic acid (ARA, 20:4n-6) and dihomo-γ-linolenic acid (DGLA, 20:3n-6) are omega-6 long-chain polyunsaturated fatty acids (LC-PUFA), which are key precursors for lipid mediators of the immune system and inflammatory response. The microalga Lobosphaera incisa (WT) and its Δ5-desaturase mutant P127 (MUT) are unique photosynthetic sources for ARA...
Article
Lobosphaera incisa is a green microalga that accumulates high levels of the valuable omega-6 long-chain polyunsaturated fatty acids (LC-PUFA) arachidonic acid (ARA, 20:4n-6) in triacylglycerols (TAG) under nitrogen (N) starvation. LC-PUFA accumulation is a rare trait in photosynthetic microalgae with insufficiently understood physiological signific...
Article
The green oleaginous microalga Lobosphaera incisa accumulates storage lipids triacylglycerols (TAG) enriched in the long-chain polyunsaturated fatty acid arachidonic acid under nitrogen (N) deprivation. In contrast, under phosphorous (P) deprivation, the production of the monounsaturated oleic acid prevails. We compared physiological responses, ult...
Article
Lipid droplets (LDs) are an organelle conserved amongst all eukaryotes, consisting of a neutral lipid core surrounded by a polar lipid monolayer. Many species of microalgae accumulate LDs in response to stress conditions, such as nitrogen starvation. Here we report the isolation and proteomic profiling of LD proteins from the model oleaginous penna...
Article
Full-text available
The green microalga Lobosphaera incisa accumulates triacylglycerols (TAGs) with exceptionally high levels of long-chain polyunsaturated fatty acid (LC-PUFA) arachidonic acid (ARA) under nitrogen (N) deprivation. Phosphorous (P) deprivation induces milder changes in fatty acid composition, cell ultrastructure, and growth performance. We hypothesized...
Article
Full-text available
CRISPR/Cas9-mediated genome editing has been demonstrated in the model diatom P. tricornutum, yet the currently available genetic tools do not combine the various advantageous features into a single, easy-to-assemble, modular construct that would allow the multiplexed targeting and creation of marker-free genome-edited lines. In this report, we des...
Article
Monogenean parasites impose a major constraint on sustainable aquacultural production and trade due to fish morbidity and mortality. Traditional chemical treatments carry environmental and human health risks and are frequently banned in aquaculture; associated development of pathogen resistance is also common. These highlight the need for alternati...
Article
Overuse of agrochemicals is linked to nutrient loss, greenhouse gases (GHG) emissions, and resource depletion thus requiring the development of sustainable agricultural solutions. Cultivated microalgal biomass could provide such a solution. The environmental consequences of algal biomass application in agriculture and more specifically its effect o...
Article
A barrier to realizing Nannochloropsis oceanica’s potential for omega-3 eicosapentaenoic acid (EPA) production is the disparity between conditions that are optimal for growth and those that are optimal for EPA biomass content. A case in point is temperature: higher content of polyunsaturated fatty acid (PUFA), and especially EPA, is observed in low...
Article
Full-text available
Several green algae can divide by multiple fission and spontaneously synchronize their cell cycle with the available light regime. The yields that can be obtained from a microalgal culture are directly affected by cell cycle events. Chromochloris zofingiensis is considered as one of the most promising microalgae for biotechnological applications du...
Article
Full-text available
Microalgae have been considered as a renewable source of nutritional, cosmetic and pharmaceutical compounds. The ability to produce health-beneficial long-chain polyunsaturated fatty acids (LC-PUFA) is of high interest. LC-PUFA and their metabolic lipid mediators, modulate key inflammatory pathways in numerous models. In particular, the metabolism...
Article
Full-text available
The green microalga Lobosphaera incisa accumulates long-chain polyunsaturated arachidonic acid sequestered in triacylglycerols under nitrogen (N)-starvation conditions. Many of L. incisa’s physiological and metabolic responses to N-starvation have been previously investigated. However, the temporal dynamics of the volatile organic compounds (VOCs)...
Article
Full-text available
The effect of dietary omega-6 long-chain polyunsaturated fatty acid (LC-PUFA) on host microbiome and gut associated immune function in fish is unexplored. The effect of dietary supplementation with the omega-6 LC-PUFA-rich microalga Lobosphaera incisa wild type (WT) and its delta-5 desaturase mutant (MUT), rich in arachidonic-acid and dihomo-gamma-...
Article
Full-text available
The dynamic nutrient availability and photon flux density of diatom habitats necessitate buffering capabilities in order to maintain metabolic homeostasis. This is accomplished by the biosynthesis and turnover of storage lipids, which are sequestered in lipid droplets (LDs). LDs are an organelle conserved among eukaryotes, composed of a neutral lip...
Article
The chlorophyte microalga Lobosphaera incisa was isolated from the snowy slopes of Mt. Tateyama in Japan. This microalga stores exceptionally high amounts of the omega‐6 LC‐PUFA arachidonic acid in triacylglycerols, and therefore represents a potent photosynthetic source for this essential LC‐PUFA. Assuming that freezing tolerance may play a role i...
Article
Phosphorus (P) is central to storing and transferring energy and information in living cells, including those of microalgae. Many microalgal species dwelling in low P environments are naturally equipped to take up and store P whenever it becomes available through a complex phenomenon known as "luxury P uptake." Its research is required for better u...
Poster
Full-text available
Efficient and economical selection markers are essential tools for genetic engineering and biotechnology. Till date, selection markers for Phaeodactylum tricornutum are dominated by antibiotic resistance genes of bacterial origin. Mutations in phytoene desaturase (PDS1) cause resistance to the herbicide norflurazon. Two novel PDS1 mutants, highly r...
Poster
Full-text available
We discuss multifaceted roles of lipids in operation and regulation of photoprotective mechanisms in photosynthetic cells using microalgal models with contrasting responses to environmental stresses. Stress-resilient microalgal species generally react to the stress by reducing their photosynthetic apparatus and redirecting the excessively photo-fix...
Article
Full-text available
Atherosclerosis and its complications are responsible for one in three global deaths. Nutraceuticals show promise in the prevention and treatment of atherosclerosis but require an indepth understanding of the mechanisms underlying their actions. A previous study showed that the omega-6 fatty acid, dihomo-γ-linolenic acid (DGLA), attenuated atherosc...
Article
Haematococcus pluvialis is an industrial green microalga, which presents the best photosynthetic source of the keto-carotenoid astaxanthin due to its high content, reaching over 4% of dry weight. Astaxanthin production by Haematococcus is currently achieved by a two-stage photoautotrophic process, which involves the transition of green biomass to n...
Article
Recent studies in mammalian models revealed compelling evidence that along with the intrinsic characteristics of diets, the time of their delivery could have a profound impact on their benefits. In this study, we explored a time-dependent modulation of the gut mucosal barrier by delivering diets enriched with the green microalga (Lobosphaera incisa...
Article
Full-text available
In photosynthetic organisms including unicellular algae, acclimation to and damage by environmental stresses are readily apparent at the level of the photosynthetic apparatus. Phenotypic manifestations of the stress responses include rapid and dramatic reduction of photosynthetic activity and pigment content aimed at mitigating the risk of photooxi...
Chapter
Interest in microalgal biotechnology for the production of bioactive and nutritional ingredients has increased tremendously with the urgent need for developing renewable bioresources. Microalgae represent a huge and still insufficiently tapped resource of LC-PUFA for human nutrition and health-related applications. Photosynthetic omega-3 and omega-...
Article
Full-text available
Recent developments in the genetic engineering of biotechnologically important microalgae—among them the diatom Phaeodactylum tricornutum—promise rapid progress in strain improvements for producing various valuable commodities. Nitrogen (N) depletion and starvation are commonly employed strategies to induce or enhance the formation of secondary car...
Article
Full-text available
We report the identification and characterization of an acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1)-encoding gene from the green oleaginous microalga Lobosphaera incisa (SAG 2468), a prolific photosynthetic producer of the n-6 very long chain polyunsaturated fatty acid (VLC-PUFA), arachidonic acid. The gene expression pattern of LiDGAT1 in L....
Poster
Full-text available
In photosynthetic organisms including unicellular algae, acclimation to and damage by environmental stresses are readily apparent at the level of cell ultrastructural organization and, particularly, organization of the photosynthetic apparatus and cell inclusions. Although the physiological and molecular mechanisms of these processes are well known...
Chapter
In response to oxidative stress the biosynthesis of the ROS scavenger, glutathione is induced. This requires the induction of the sulfate reduction pathway for an adequate supply of cysteine, the precursor for glutathione. Cysteine also acts as the sulfur donor for the sulfuration of the molybdenum cofactor, crucial for the last step of ABA biosynt...
Chapter
The amino acid cysteine plays a major role in plant response to abiotic stress by being the donor of elemental sulfur for the sulfuration of the molybdenum cofactor, otherwise the last step of ABA biosynthesis, the oxidation of abscisic aldehyde, is inactivated. Additionally, cysteine serves as a precursor for the biosynthesis of glutathione, the r...
Article
Full-text available
Background Lobosphaera incisa (L. incisa) is an oleaginous microalga that stores triacylglycerol (TAG) rich in arachidonic acid in lipid bodies (LBs). This organelle is gaining attention in algal research, since evidence is accumulating that proteins attached to its surface fulfill important functions in TAG storage and metabolism. Results Here, t...
Article
The oleaginous microalga Lobosphaeraincisa(Trebouxiophyceae, Chlorophyta)contains arachidonic acid (ARA, 20:4 n-6) in all membrane glycerolipids and in the storage lipidtriacylglycerol.The optimal growth temperature of the wild-type (WT) strain is 25°C; chilling temperatures (≤15oC) slow its growth. This effect is more pronounced in the delta-5-des...
Article
Full-text available
Oleaginous microalgae are considered as a promising resource for the production of biofuels. Especially diatoms arouse interest as biofuel producers since they are most productive in carbon fixation and very flexible to environmental changes in the nature. Naturally, triacylglycerol (TAG) accumulation in algae only occurs under stress conditions li...
Data
Changes of different lipid classes during the growth kinetic. Absolute amounts (μmol/g) are given for triaclyglycerol (TAG), diacylglycerol (DAG), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), sulfoquinovosyldiacylglycerol (SQDG), phosphatidylglycerol (PG), diacylglyceroltrimethyhomoserine (DGTS), phosphatidylcholine (PC),...
Data
Principle component analysis (PCA) of the metabolome of Phaeodactylum tricornutum under replete and N-deplete conditions. Phaeodactylum tricornutum cultures grown under replete condition and normal light (NL), or grown under N-deplete conditions (-N) and normal light (NL) or high light (HL) were harvested at the indicated time points and extracted...
Data
Comparison of the total amount of the different lipid classes under replete and N-deplete conditions. The total lipid content of the major lipid classes of the last time point of growth kinetic was calculated from the values shown in Figs 2 and 3. Triacylglycerol (TAG), diacylglycerol (DAG), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylgl...
Data
High resolution MS/MS analyses by UHPLC-ESI-QTOF-MS. Shown are fragmentation patterns at 10–15 eV collision energies in positive (DMSP, dimethylsulfoniobutyrate and O-propanoyl-carnitin) or negative (methylsulfate) ionization mode, as well as the corresponding chemical formula for each compound based on the fragmentation pattern. (TIF)
Data
Raw data table of the lipid analysis. In this table, the raw data of the different lipid molecular species are listed. The table is divided in absolute amounts [μmol/g] and relative values [%]. (XLSX)
Data
Raw data table–Metabolite fingerprinting. (XLSX)
Data
Raw data table of metabolites. Trimethylsilyl (TMS) and methoxyamine (MEOX) derivatives of the metabolites were analysed by GC-MS. Samples were measured in two (timepoint 0 d) or three biological replicates (all other samples). The standard deviation (SD) is given. Metabolites were identified either by their retention time and spectra compared with...
Data
Changes of different lysolipids during the growth kinetic. Absolute amounts (μmol/g) are shown for monogalactosylmonoacylglycerol (MGMG), digalactosylmonoacylglycerol (DGMG), lysophosphatidylglycerol (LPG), monoacylglyceroltrimethyhomoserine (MGTS) lysophosphatidylcholine (LPC), lysophosphatidyethanolamine (LPE) and lysophosphatidylserine (LPS). Fo...
Data
Main molecular species of each lipid class under different conditions. In this table, the major molecular species for each lipid class and condition are given as relative value. (TIF)
Data
FA profile of neutral lipids (A), glycolipids (B) and phospholipids (C). The diagrams shows day 0 and the last time point of each condition. Day 0 comprises the mean of all conditions (beige). Day 7 of replete conditions is shown in blue, day 6 of N-deplete with normal light in red and day 6 of N-deplete with high light in green. Data are mean valu...
Data
Raw data table of analytes. Trimethylsilyl (TMS) and methoxyamine (MEOX) derivatives of the metabolites were analysed by GC-MS. Samples were measured in two (timepoint 0 d) or three biological replicates (all other samples). Values were calculated from the integration results using the quantification mass to charge ratio (quant m/z) at the retentio...
Article
Microalgae accumulate the storage lipids triacylglycerols (TAG) in lipid droplets (LDs) in response to environmental cues. Mechanistic insights into LD biogenesis in microalgal cells hold great promise for the manipulation of TAG production. In this report, we demonstrate the heterologous expression of a major LD protein of the green microalga Haem...
Article
Full-text available
The salinization of land is a major factor limiting crop production worldwide. Halophytes adapted to high levels of salinity are likely to possess useful genes for improving crop tolerance to salt stress. In addition, halophytes could provide a food source on marginal lands. However, despite halophytes being salt-tolerant plants, the seeds of sever...
Poster
Background: The oleaginous green alga Lobosphaera incisa accumulates high amounts of arachidonic acid (ARA) rich triacylglycerols (TAG) in cytoplasmic lipid droplets (LDs), predominantly under nitrogen starvation conditions. Intensive LD formation is accompanied by dismantling of chloroplast and endomembrane systems and recycling of cellular compon...
Chapter
Microalgae have gained the attention of the scientific community, particularly lipid biochemists and microalgal biotechnologists, as a source of valuable nutritional ingredients, such as long-chain polyunsaturated fatty acids (LC-PUFA) and carotenoids, as well as precursors for biodiesel production. The field of microalgal lipids, particularly with...
Article
Microalgae present a huge and still insufficiently tapped resource of very long-chain omega-3 and omega-6 polyunsaturated fatty acids (VLC-PUFA) for human nutrition and medicinal applications. This chapter describes the diversity of unicellular eukaryotic microalgae in respect to VLC-PUFA biosynthesis. Then, we outline the major biosynthetic pathwa...
Article
Full-text available
Plant sulfite oxidase [SO; E.C.1.8.3.1] has been shown to be a key player in protecting plants against exogenous toxic sulfite. Recently we showed that SO activity is essential to cope with rising dark-induced endogenous sulfite levels in tomato plants (Lycopersicon esculentum/Solanum lycopersicum Mill. cv. Rheinlands Ruhm). Here we uncover the ram...
Article
Full-text available
Lobosphaera incisa, formerly known as Myrmecia incisa and then Parietochloris incisa, is an oleaginous unicellular green alga belonging to the class Trebouxiophyceae (Chlorophyta). It is the richest known plant source of arachidonic acid, an ω-6 poly-unsaturated fatty acid valued by the pharmaceutical and baby-food industries. It is therefore an or...
Article
Full-text available
The oleaginous green microalga Lobosphaera (formerly Parietochloris) incisa accumulates high amounts of arachidonic-acid-rich triacylglycerols (TAG), in particular under conditions of nitrogen starvation. This photosynthetic organism is of great interest for studying the mechanisms responsible for storage lipid biosynthesis and the deposition of lo...
Article
Full-text available
Nannochloropsis oceanica is an important source for omega-3 long-chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic acid (EPA, 20:5 n − 3), and a potent candidate for biofuel production, due to its outstanding capability for rapid induction of triacylglycerol (TAG) overproduction. In contrast to membrane lipids, TAG of N. oceanica...