Inga Morkvenaite-Vilkonciene

Inga Morkvenaite-Vilkonciene
Vilnius Gediminas Technical University | VGTU · Department of Mechatronics Robotics and Digital Manufacturing

PhD Chemistry

About

69
Publications
15,119
Reads
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527
Citations
Introduction
I have a PhD in Chemistry, and master degree in Control Engineering. I am interested in biological materials, such as living cells, investigation with Scanning Electrochemical Microscopy and Atomic Force Microscopy.
Additional affiliations
February 2022 - May 2022
State research institute Center for Physical Sciences and Technology
Position
  • Chief researcher
Education
September 2009 - January 2011
Kaunas University of Technology
Field of study
  • Electrical Engineering

Publications

Publications (69)
Article
Over the past several decades, biology, chemistry, and medicine took advantage of gold nanoparticles (AuNPs) for their fascinating characteristics. Today these nanomaterials can be synthesized in various methods from biosynthesis, when particles are made by microbial culture, to physical. Using the fast scan cyclic voltammetry, AuNPs can be electro...
Article
Scanning electrochemical microscopy (SECM) is a local electrochemical analysis technique, which performs scanning of the sample by ultramicroelectrode (UME) [1]. The result of measurement usually is current, which depends on the electrochemical properties of the surface of interest. The errors can be observed if UME geometry is not ideal [2,3]. Thi...
Article
Full-text available
Recent industrial robotics covers a broad part of the manufacturing spectrum and other human everyday life applications; the performance of these devices has become increasingly important. Positioning accuracy and repeatability, as well as operating speed, are essential in any industrial robotics application. Robot positioning errors are complex du...
Article
BiVO4 is known as a promising material for the design of analytical systems dedicated for non-enzymatic photoelectrochemical (PEC) glucose determination. In this work BiVO4 coatings were deposited on fluoride-doped tin oxide substrate by simple sol-gel method. The influence of different stabilizers, such as polyethylene glycol, urea, citric acid an...
Article
Full-text available
This review is dedicated to the development of molecularly imprinted polymers (MIPs) and the application of MIPs in sensor design. MIP-based biological recognition parts can replace receptors or antibodies, which are rather expensive. Conducting polymers show unique properties that are applicable in sensor design. Therefore, MIP-based conducting po...
Article
Full-text available
Electrically conductive polymers are promising materials for charge transfer from living cells to the anodes of electrochemical biosensors and biofuel cells. The modification of living cells by polypyrrole (PPy) causes shortened cell lifespan, burdens the replication process, and diminishes renewability in the long term. In this paper, the viabilit...
Chapter
Fast and sensitive determination of biomarkers and biologically active compounds is very important in biomedical diagnostics, food and beverage industry, and environmental analysis. Some promising directions in analytical and bioanalytical application of conducting polymers (CPs) are discussed. Recently polypyrrole, polyaniline, polythiophene, and...
Article
Full-text available
Paper provides an attempt to create a methodology for automated structure health monitoring procedures using vibration spectrum analysis. There is an option to use autoregressive (AR) spectral analysis to extract information from frequency spectra when conventional Fast Fourier transformation (FFT) analysis cannot give relevant information. An auto...
Article
Full-text available
Human falls pose a serious threat to the person’s health, especially for the elderly and disease-impacted people. Early detection of involuntary human gait change can indicate a forthcoming fall. Therefore, human body fall warning can help avoid falls and their caused injuries for the skeleton and joints. A simple and easy-to-use fall detection sys...
Article
Full-text available
Scanning electrochemical microscopy enhanced by electrochemical impedance spectroscopy (SEIM) was applied to detect immobilized antibodies labelled with horseradish peroxidase (Ab-HRP). The localized HRP activity was investigated by the SEIM redox competition (RC-SEIM) mode using hydrogen peroxide as a substrate and hexacyanoferrate as a redox medi...
Article
In this research, scanning electrochemical microscopy (SECM) and electrochemical impedance spectroscopy (EIS) were applied for the evaluation of surface characteristics of electrochemical (amperometric) biosensor based on two composite structures consisting of perforated polycarbonate membrane modified by carbon-nanomaterials and glucose oxidase. T...
Article
Full-text available
Microbial fuel cell (MFC) efficiency depends on charge transfer capability from microbe to anode, and the application of suitable redox mediators is important in this area. In this study, yeast viability experiments were performed to determine the 2-methyl-1,4-naphthoquinone (menadione (MD)) influence on different yeast cell species (baker’s yeast...
Article
Microbial fuel cells can be efficiently used for simultaneous cleaning of wastewater and generation of electricity. This research demonstrates the applicability of Baker yeast cells in the design of microbial biofuel cells. The applicability the 9,10-phenantrenequinone (PQ) as a redox mediator in the design of yeast-based microbial cell (MFC) for t...
Article
Full-text available
The progress observed in ‘soft robotics’ brought some promising research in flexible tactile, pressure and force sensors, which can be based on polymeric composite materials. Therefore, in this paper, we intend to evaluate the characteristics of a force-sensitive material—polyethylene-carbon composite (Velostat®) by implementing this material into...
Article
Three-dimensional (3D) cell cultivation systems in vitro are promising biomodels to study the metabolic processes of healthy and diseased human heart cells. Cardiac 3D-spheroids are mimicking cell living conditions and, therefore, they can be employed to investigate processes that occur in both extra-cellular and intra-cellular environments. In thi...
Article
Full-text available
This research aimed to evaluate the toxic effect of multi-walled carbon nanotubes (MW-CNTs) on yeast cells in order to apply MW-CNTs for possible improvement of the efficiency of microbial biofuel cells. The SEM and XRD analysis suggested that here used MW-CNTs are in the range of 10–25 nm in diameter and their structure was confirmed by Raman spec...
Chapter
The increase in energy consumption also increases the damage to the environment and the toxicity of water still is one of the main problems in the world. Microbial fuel cells (MFCs) are one of the alternative energy sources. The study demonstrates the possibility to use yeast as a probe to assess redox and electrophile-based toxicities and to exten...
Chapter
Full-text available
Positioning accuracy of articulated robots decreases significantly when they are fully loaded and operated at maximum speeds due to increased inertia. Hard-coding correction algorithms using traditional methods is extremely difficult. A system, which could automatically detect patterns in deviations and offer possible corrections at every motion cy...
Chapter
In order to achieve an accurate positioning for an investigation of micro- or nano objects or to operate with them in micro or nanoscale, modern and sophisticated tools are required. Over the past years of the development of micro- and nanomanipulation tools, novel types were invented, or old ones were upgraded. Those tools such as microgrippers, m...
Article
Full-text available
In this study the redox activity of human myocardium‐derived mesenchymal stem cells (hmMSC) were investigated by redox‐competition (RC‐SECM) and generation‐collection (GC‐SECM) modes of scanning electrochemical microscopy (SECM), using 2‐methylnaphthalene‐1,4‐dione (menadione, MD) as a redox mediator. The redox activity of human healthy and dilated...
Chapter
Living cells mechanical properties establishment from Atomic force microscopy (AFM) force-separation curves is a challenge because the calculated Young’s modulus depends on the applied mathematical model. The more reliable results can be obtained using finite element models. In this work, yeast cells with different mechanical properties were measur...
Chapter
Atomic force microscope (AFM) is a promising tool in micro and nano size objects researches. Contact mode AFM has advantages comparing to non-contact modes: the scanning speed is higher, and atomic resolution can be achieved. The main limiting factor in contact mode AFM is scanning speed. At high scanning speed the ‘loss of contact’ phenomenon occu...
Article
Scanning electrochemical microscopy (SECM) is very useful, non-invasive tool for the analysis of surfaces pre-modified with biomolecules or by whole cells. This review focuses on the application of SECM technique for the analysis of surfaces pre-modified with enzymes (horseradish peroxidase, alkaline phosphatase and glucose oxidase) or labelled wit...
Article
Scanning electrochemical microscopy (SECM) is an emerging electroanalytical sensing technique, used to investigate the electrochemical properties of the sample by ultra‐micro‐electrode(UME) scanning probe. UME signal usually is the current, which depends not only on the properties of the evaluated system but also on UME characteristics such as geom...
Article
In this study a polycarbonate filter membrane (PcFM) with 400 nm diameter holes was covered/protruded by single walled carbon nanotubes (SWCNT) and then formed PcFM/SWCNT structure was covered by thin layer of graphene oxide (GO) or reduced graphene oxide (rGO) in order to get the multilayered PcFM/SWCNT/GO and PcFM/SWCNT/rGO coatings, respectively...
Conference Paper
Electrochemical impedance spectroscopy (EIS) is widely used in various bioelectrochemical researches. Conventional EIS techniques gives only average response of the whole system. In order to observe localized EIS, the working electrode has to be moved in close to the surface of interest. In this case, scanning electrochemical microscopy (SECM) [1]...
Article
The dynamics of atomic force microscope (AFM) acting in contact mode is crucial for correct interpretation of AFM images and especially for the identification of artefacts and the determination of their sources. This paper presents a methodology for the interpretation and improvement of the AFM image quality. The developed AFM dynamic model has bee...
Article
Full-text available
In order to maintain competitiveness and a technical edge business entity are increasingly implementing advanced technical solutions in their operational processes, most of which include the installation of various type robotic systems. One of the best known and widely distributed examples of universal robotic system is Kuka-Youbot, which is a modu...
Article
Full-text available
Scanning electrochemical microscopy (SECM) hybridized with fast Fourier transform-based electrochemical impedance spectroscopy (FFT-EIS) seems to be a powerful variation of scanning electrochemical impedancemicroscopy (SEIM), wherein both state-of-the-art techniques are combined (FFT-SEIM) and can be used forthe investigation and treatment of tissu...
Article
In this research, we are reporting the application of microorganisms modified by a conducting polymer – polypyrrole (Ppy) – for the improvement of microbial biofuel cells. The synthesis of polypyrrole was assisted by metabolic and other biochemical processes that occur in microorganisms. Therefore, in this research the reported Ppy formation method...
Article
Full-text available
Increasing the imaging rate of atomic force microscopy (AFM) without impairing of the imaging quality is a challenging task, since the increase in the scanning speed leads to a number of artifacts related to the limited mechanical bandwidth of the AFM components. One of these artifacts is the loss of contact between the probe tip and the sample. We...
Article
Elastic modulus of the furniture coatings is an important parameter, which indicates the curing state of the composite resin curable by the ultraviolet radiation. Non-contact way of elastic modulus measurement by the transverse acoustical waves, propagating perpendicular to the surface of a 10- $\mu \text{m}$ thick polymer, is shown as a suitable...
Preprint
Increasing of the imaging rate of conventional atomic force microscopy (AFM) is almost impossible without impairing of the imaging quality, since the probe tip tends to lose contact with the sample. We propose to apply the additional nonlinear force on the upper surface of a cantilever, which will help to keep the tip and surface in contact. In pra...
Article
Cadmium selenide (CdSe)-based layers were formed on the surface of polyamide 6 (PA) substrate film by treating PA film samples with potassium selenotrithionate (K2SeS2O6) solution (seleniumization) and then with cadmium (II) acetate, Cd(CH3COO)2, solution (cadmiumization). The morphology of CdSe-based layers was investigated by atomic force and sca...
Article
Positioning problems of scanning electrochemical microscopy (SECM) are very important by means of living cells damaging by moving ultramicroelectrode (UME). The working principle and operating modes of SECM are introduced. Investigation of redox activity of living cells are outlined. Problems, which arise in investigations of living cells by consta...
Article
Fast Fourier transform electrochemical impedance spectroscopy (FFT-EIS) is a technique that simultaneously applies 30–50 frequencies and simultaneously all data of EIS spectra at these frequencies are registered. Therefore EIS spectra can be acquired many times faster compared to experiments performed by conventional EIS equipment. Scanning electro...
Article
Glucose oxidase (GOx) is an enzyme, which is used for the development of enzymatic biofuel cells. Therefore in this research redox competition mode of scanning electrochemical microscopy (RC-SECM) was applied for the investigation of glucose oxidase (GOx) catalyzed reaction kinetics. The GOx was immobilized by glutaraldehyde on substrates of differ...
Article
Full-text available
Shock absorbers allow the damping of suspension vibrations, by dissipating kinetic energy. This energy theoretically can be harvested; however, practical solutions are not easily obtainable. This paper is dedicated to analyzing and evaluating the vibration energy in a vehicle's suspension that is generated by road excitations. Also, it estimates th...
Conference Paper
SWCNT coatings (~ 400 nm thickness) on the polycarbonate substrate were prepared having aim to use them as electrodes for amperometric biosensors. These coatings were modified using an additional coverage with graphene oxide (GO) flakes (SWCNT-GO coatings) and subsequent annealing (SWCNT-rGO coatings). Produced coatings were investigated in order t...
Article
Magnetorheological (MR) dampers are currently used in various areas, such as: human prosthetics, seismic protection, active suspensions, safety systems, amongst other. This paper deals with the proper design of a MR damper in the innovative field for vibration control. A methodology for calculation some principal characteristics of the damper such...
Conference Paper
EIS is a powerful, non-destructive and informative technique, which has been successfully applied for the characterization of biosensor surfaces [1]. However, conventional EIS based techniques represent only averaged response of the entire electrochemical system. In order to get more advanced mapping of electrochemical system scanning electrochemic...
Article
In this research scanning electrochemical microscopy was applied for the investigation of immobilized yeast Saccharomyces cerevisiae cells. Two redox mediators based system was applied in order to increase the efficiency of charge transfer from yeast cells. 9,10-phenanthrenequinone (PQ) was applied as a lipophilic redox mediator, which has the abil...
Article
Scanning electrochemical microscopy (SECM) is an advanced electrochemical method, which is based on electrochemical scanning of electrochemical system surface by an ultramicroelectrode. Due to very high complexity of such systems the evaluation of data collected by this electrochemical method is a challenging task, which could be partly solved by m...
Thesis
Annotation Scanning electrochemical microscopy (SECM) is an innovative method, which could be applied for the surface-activity analysis of enzymatic biosensors and biofuel cells. The SECM is based on electrochemical measurements performed by ultramicroelectrode (UME), which is scanning the 3D space, which is close to surface of the interest that is...
Conference Paper
Localized evaluation of bio-electrocatalytic activity of redox enzymes immobilized on the surface could be very attractive for biosensor design or development of biofuel cells3. Scanning electrochemical microscopy (SECM) is an innovative method, which could be applied for the surface-activity analysis of enzymatic biosensors. Initially the SECM was...
Conference Paper
Demographic changes, multiple and chronic diseases as well as possibilities provided by the modern information technology to compare and benchmark own health parameters at patient networks and databases are the drivers of coming health care innovations. To meet required safety and accuracy of medical services, at point-of-care units and, especially...
Article
Yeast cells Saccharomyces cerevisiae were investigated by scanning electrochemical microscopy (SECM) at generation-collection mode. A two redox mediator based system was designed and adapted in order to evaluate redox activity of yeast cells. One redox mediator was 9,10-phenanthrenequinone (PQ), which was lipophilic and was incorporated within the...
Article
In this research biointerface based on immobilized glucose oxidase (GOx) was evaluated by scanning electrochemical impedance microscopy (SEIM), which consisted of merged scanning electrochemical microscopy (SECM) and electrochemical impedance spectroscopy (EIS). The gluconolactone, which is quickly hydrolyzed to gluconic acid, is produced during th...
Article
Full-text available
Redox competition (RC-SECM) and generation-collection (GC-SECM) modes of scanning electrochemical microscopy were applied for the evaluation of glucose oxidase (GOx) modified not conducting poly(methyl methacrylate) surface. The current vs distance curves in RC-SECM mode were registered at -600 mV vs Ag/AgCl in order to determinate local O2 concent...
Article
Atomic force microscopy is a valuable and useful tool for the imaging and investigation of living cells in their natural environment at high resolution. Procedures applied to living cell preparation before measurements should be adapted individually for different kinds of cells and for the desired measurement technique. Different ways of cell immob...
Article
Full-text available
CMUT FEM 2D electrostatic model was created to investigate dielectric surface roughness influence to the operating point. Three different form structures: triangle, half-circle, rectangle were investigated. The highest electric field norm concentration on the triangle apex was found. For experiments, dielectric surface was etched by two etching ste...
Article
The paper presents an investigation of formation optical structures with electron beam on dielectric background. Problem with dielectric background was resolved to covering polymer (PMMA) with silver layer.
Conference Paper
In-depth knowledge of scanning probe microscopy (SPM) dynamics is necessary when interpreting obtained images and especially when the sources of artifacts are to be identified. The aim of this research was to build and test the dynamic model of SPM electromechanical system. For simplicity reasons only atomic force microscopy (AFM) contact mode dyna...

Questions

Questions (5)
Question
In the many publications and books authors write that the probe diameter is the most important factor for resolution in range of nanometers. We have probes with 10 nm diameter, it is possible to vizualize enzymes with such "big" probe?
Question
I work with Scanning Electrochemical Microscope. On the plastic surface I have enzyme layer (glucose oxidase), immobilized by glutaraldehyde. Then I add glucose, the current increase and this is alright. But with bigger concentration of mediator (Fe(CN)6) the current decreases. Why is this happening? I have performed the experiment three times and always get the same result.
Question
Maybe someone has experience in biological surfaces measurement in AC-mode SECM? I am interested in measuring properties of immobilized enzyme, but any experience can be useful for me.
Question
Measurement is performed by scanning electrochemical microscope with ultramicroelectrode (10 um) on the GOx.
Question
Approach curve is measured by a scanning electrochemical microscope with an ultramicroelectrode (10 um) on the biological surface - GOx.

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