Inês Diniz- PhD
- Pos-Doc at University of Lisbon
Inês Diniz
- PhD
- Pos-Doc at University of Lisbon
About
38
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Introduction
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October 2007 - August 2008
Publications
Publications (38)
Coffee is one of the most important cash crops and beverages. Several diseases caused by fungi, bacteria, and viruses can affect coffee plantations and compromise production. Coffee leaf rust (CLR), caused by the biotrophic fungus Hemileia vastatrix is the top fungal disease, representing a permanent threat to sustainable Arabica coffee production...
Plants have evolved sophisticated mechanisms to coordinate carbon metabolism during growth and development both under optimal and stress conditions. In coffee-rust biotrophic interactions, plants try to limit pathogen access to nutrients (e.g., sugars and sugar derivatives) and trigger immune responses, while Hemileia vastatrix (Hv) attempts to cir...
Coffee leaf rust (CLR), a disease caused by the biotrophic fungus Hemileia vastatrix (Hv), is the main threat to the worldwide production of Arabica coffee. The gradual breakdown of resistance in coffee varieties in the last years has highlighted the need for novel sources of resistance to CLR. This work aimed to unveil the cellular and molecular r...
The biotrophic fungus Erysiphe necator causes powdery mildew (PM) in grapevine. Phytohormones are major modulators of defensive responses in plants but the analysis of the hormonome associated with grapevine tolerance and susceptibility against this pathogen has not been elucidated. In this study, changes in hormonal profiling were compared between...
The harvesting, processing, and sale of wild edible mushrooms (WEM) is a relevant economic activity in Angola and a good example of the use of non-wood forest products for food. Although there is deep traditional knowledge about the general properties of WEMs, a huge gap remains in detailed scientific knowledge. Thus, this study aimed to investigat...
The biotrophic fungus Erysiphe necator causes powdery mildew (PM) in grapevine. Phytohormones are major modulators of defensive responses in plants but the analysis of the hormonome associated with grapevine tolerance and susceptibility against this pathogen has not been elucidated. In this study, changes in hormonal profiling were compared between...
Coffee leaf rust (Hemileia vastatrix) is the most important disease of Coffea arabica. This project aims to identify coffee’s primary metabolic components essential for growth and development that are simultaneously involved in plant defense responses. We studied the interaction of two contrasting coffee genotypes [C. arabica S4 Agaro and Kawisari...
Dear Colleagues,
The increase in the world population and its inescapable need for food is in an armed fight with dramatic climatic changes, limited water, and land resources, and scarcity of agricultural resources. In this challenging scenario, phytopathogenic agents can have a severe social-economic impact on global agriculture if not well manag...
Grapevine trunk diseases constitute one of the major problems for viticulture worldwide, with Botryosphaeria dieback considered as one of the most important of these diseases. In this work, we aimed to (i) evaluate the effect of the combination of two products, Esquive® (a Trichoderma-based product) and LC2017 (a low-copper-based product), in the c...
O presente manual de apoio pretende constituir uma ferramenta para facilitar a identificação de doenças, pragas e infestantes nos cajueiros na Guiné-Bissau, através de uma breve descrição da sintomatologia e dos órgãos afetados na planta, devidamente apoiada por fotografias ilustrativas.
Coffee is one of the most consumed beverages in the world. It is also one of the most globally traded commodities. Coffee leaf rust (CLR), caused by the biotrophic fungus Hemileia vastatrix, is the most important disease affecting Arabica coffee growing worldwide, leading to significant yield losses if no control measures are applied. A deep unders...
Figure S1. Maximum likelihood phylogenetic tree obtained from the concatenated dataset of b-tubulin, ITS and EF1-a.
Figure S1. Maximum likelihood phylogenetic tree obtained from the concatenated dataset of b-tubulin, ITS and EF1-a.
Cashew (Anacardium occidentale L.) is a cash crop with a highly significant economic importance in West Africa, particularly in Guinea-Bissau (Monteiro et al. 2015, 2017). In October 2018, dieback-like symptoms such as wilt and necrosis of apical shoots were observed in 10 % of the cashew trees grown in a 100 plant-orchard in Bolama Island at Bijag...
Hormones play an important role in response to fungal pathogens. Nevertheless, analyses of hormonal profiling during defense are scarce. In this work, changes in hormonal metabolism in grapevine were compared between a susceptible (Vitis vinifera cv. Aragonês) and a tolerant (Vitis rupestris × riparia) species under infection with powdery mildew. T...
https://www.ecfg15.org/wp-content/uploads/2020/03/ECFG15-Program_and_Abstracts.pdf
Colletotrichum kahawae is a specialized plant pathogen of arabica coffee in Africa, able to infect green berries. The economic impact of this pathogen means there is an urgent need to better understand its pathogenic lifestyle, in particular its aggressiveness. In this study,
several quantitative traits including disease severity, latent period and...
A mosaic of traditional agriculture and extensive plantations of cash crops, which alter the natural ecosystems, characterize agricultural landscapes in West Africa. Cashew (Anacardium occidentale L.) is a tropical tree native to South America, currently cultivated in most tropical countries in the world. Among the main cashew production areas, Wes...
Executive Summary:
Cashew is a major agriculture commodity in Guinea-Bissau, not only providing significant export earnings but also on households' income and food security at smallholder level. Thus, evaluation of the cashew orchards enemies is of chief importance to the sustainable production in this crop. With this project, we aim at characteri...
Resistant and susceptible coffee varieties were used aiming to unveil genes and proteins putatively involved in the resistance response to Colletotrichum kahawae. Gene expression analysis revealed a stronger induction of recognition, signaling and cell wall modification genes in the resistant variety during fungal penetration. It was also observed...
Understanding the molecular mechanisms underlying coffee-pathogen interactions are of key importance to aid disease resistance breeding efforts. In this work the expression of genes involved in salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) pathways were studied in hypocotyls of two coffee varieties challenged with the hemibiotrophic fun...
Schematic diagram of the current models of the SA, JA and ET pathways.
a) SA pathway—SA is synthesized from chorismate through two distinct enzymatic pathways: PAL-mediated phenylalanine and ICS-mediated isochlorismate (IC). SA-induced redox changes lead to the reduction of inactive NPR1 oligomers to active monomers that are translocated into the n...
Dissociation curves for non-specific qPCR products analysis of SA, JA, ET pathways related genes and qPCR reference genes: a) PAL, b) ICS2, c) NPR1, d) PR1, e) OPR3, f) COI, g) PR10, h) ACO2, i) ACS5, j) ETR1, k) EIN2, l) CTR1, m) ERF1, n) IDE, o) β-Tub9, p) S24.
(PUB)
qPCR expression analysis of SA pathway associated genes.
Relative expression pattern of a) PAL/ICS2 (biosynthesis), b) NPR1 (receptors), and c) PR1 (responsive gene) obtained in Catimor 88 (R-resistant) and Caturra (S-susceptible) coffee varieties. Mean and standard deviation of three biological replicates is presented. Fold change as relative expr...
Coffee Berry Disease caused by the hemibiotrophic fungus Colletotrichum kahawae (Ck) is a major constraint for Arabica coffee production in Africa and represents a threat for cultivation in America and Asia. Although there are coffee genotypes known to be resistant to Ck, the molecular genetic basis of the resistance is still unknown. With the purp...
Taxonomy and history:
Hemileia vastatrix Berk. and Broome (Basidiomycota, Pucciniales) was described in 1869 in eastern Africa and Ceylon as the agent of coffee leaf rust and has spread to all coffee cultivation areas worldwide. Major disease outbreaks in Asia, Africa and America caused and continue to cause severe yield losses, making this the mo...
Cytological, biochemical and molecular studies were undertaken to elucidate the role of oxidases in coffee resistance to Colletotrichum kahawae (Ck). Hypocotyls of the coffee variety Catimor 88, resistant to Ck isolate Que2 (from Kenya), were used and compared with the susceptible variety Caturra. Coffee resistance was characterized by a restricted...
Black foot of grapevine is an important disease caused primarily by Ilyonectria spp. and "Cylindrocarpon" pauciseptatum. These pathogens affect grapevine nurseries and young vineyards, causing the decline and death of plants. In the nursery, the primary infections of the grafted cuttings are mainly attributed to soil-borne inoculum, which could inf...
The decline of cork oak (Quercus suber) stands in Iberian Peninsula is associated with infection by Phytophthora cinnamomi. Most Phytophthora spe-cies secrete elicitins, which can enhance defence reac-tions against some pathogens. Here cytological and physiological effects of the elicitins cryptogein and capsicein on cork oak root infection by P. c...
In Arabica coffee breeding, some of the most used sources of resistance to leaf rust (Hemileia vastatrix) are natural Coffea arabica x canephora hybrids (“Híbrido de Timor”). To decipher the cellular and molecular nature of that resistance, leaves of genotype HDT832/2,
were challenged with H. vastatrix race II, and monitored using light microscopy...
Coffee plants are seriously affected by leaf rust (Hemileia vastatrix) but are naturally immune to other rusts. In fact, non-host resistance (NHR) is among the most durable forms of disease resistance, making the study of non-host interactions of great importance to understand the mechanisms of disease resistance and to improve its durability. This...
The oomycete P. cinnamomi has been described as strong contributing factor to the decline of cork oak and holm oak stands occurring in the Iberian Peninsula. There are no eradication methods available against this pathogen. Molecular mechanisms of oomycete — host interaction are not well understood, but it is known that oomycete species can induce...
Questions
Questions (8)
Hello everyone.
Please help me to understand this agarose gel. I did a plasmid (Pgem vector with an insert with an expected size of about 3kb, from E.coli) extraction with a simple protocol Birnboim e Doly (1979). The first two bands are the different topologies of the plasmid. What I need to know is the last band (very intense) - It's very small and lower than 100bp (maker is 100bp plus). What could it be? Thanks
Hello everyone,
Has anyone ever change the bulb (lamp) in a iCycler iA iQ5 bio-rad? Bio-rad does not sell this lamp anymore, and I'm searching for an alternative solution. However a bulb with this specifications (EXN, 50w , 12v halogen WITHOUT front glass) is very (but VERYYY) difficult to find (portugal).
Can anyone give me suggestions of web sites (Europe and/or UK) that could sell this like new?
thanks!!
Ddear colleagues
I'm performing a statistical analysis of real-time-PCR data by the delta delta ct method. Basically I'm analysing the expression profile of a target gene in non-inoculated (control) and infected samples, at several time points. My approach would be multifactorial anova followed by Fisher's LSD. However, I think that LSD can only be performed if population variances are equal (homogeneity of variance ). I'm using SPSS software and when I do a anova>LSD it also gives me the levene's test for (homogeneity of variance ) (levene's>anova>LSD). In my data the p value is less than 0.05 and the null hypothesis is rejected and therefore, the homogeneity of variance is rejected, and therefore, the LSD can not be applied. Is this so?
I have been reading some papers and in the statistical analysis section, is always referred the anova and LSD but no Levene's test or homogeneity of variance test.
If I know that levene's test fails for homogeneity of variance of my sample, what tests can I apply? Thanks
I have some qPCR data (raw data) from StepOne (Applied Biosystems) to be analysed using LinRegPCR. I get the error message " variant or safe array index out of bounds". What does this mean? A have followed a previous tutorial (were in researchgate) to successfully get the raw data. I have 5 columns and as many rows as the samples. When I open LineReg, I choose "Applied Biosystems (5 leading columns)"but I still get this error message...
Has anyone experienced this before?
hello everyone, i need to measure the size some conidia, but the software (leica) that comes with our microscope it's not working. Those anyone knows a free software that can do that?
Hello to all,
I have a complete genome of a plant and a fast file with multiple sequences of a specific protein ( nucleotide sequence). I want to blast the file into the genome to see if these proteins are present there. In bioedit i can blast one sequence at the time, but that is time consuming. What is the best program to do it, to take the fasta file and blast it againt the genome? I have access to DNAstar lasergene...any of the programs to it?
Thanks to all.
I need to prepare formvar-coated grids for TEM. I usually use an uncommon method of a drop of formvar on water, however the formvar film is usually uneven. Another and more common method is by using a glass slide, breath moist air and slowly lower slide into the water at ~45° angle to float off the film. Gold colour means the film is too thick, being the grey colour the best. However, I'm not getting the grey colour, and I get almost gold colour. I read that faster the lift of teh slide, the thicker the film will be. Is there any trick to get the grey colour. however i have tried to leave the slide 5 minutes in the formvar that...gold again!....anyone working in electron microscopy how that the grey colour is the COLOUR
I want to preserve some fungi by the silica gel method (with skim milk). However i can't find a reference to the ideal silica to be used. In my lab we have an orange silica (when dried) but with an irregular shape and also a blue one (when dried) with a sphere shape. What silica do you use?
