Iman Rad

Stem cell technology research center · Stem cell biology

Abstract Lipid-binding domains regulate positioning of the membrane proteins via specific interactions with phospholipid's head groups. Spinal cord injury (SCI) diminishes the integrity of neural fiber membranes at nanoscopic level. In cases that the ruptured zone size is beyond the natural resealing ability, there is a need for reinforcing factors...

Objectives: The aim of this study was to assess the possibility of transmitting the "ampicillin" growth inhibitory property to pure sterile water. Design: Three control groups were designed as references of bacterial maximal growth (MG). Different concentrations of ampicillin and pure sterile water used as "positive" and "negative" controls, res...

Interferons (IFNs) are the main part of the immune system as a defensive response to viral infection. The phosphorylation domain of Hepatitis C virus (HCV) E2 envelope glycoprotein is homologous to the host key enzymes in IFN-mediated response. So, if host kinases show no preference on E2 protein phosphorylation over IFN triggering factors, the IFN...

Recent developments in bone tissue engineering have paved the way for more efficient and cost-effective strategies. Additionally, utilization of autologous sources has been considered very desirable and is increasingly growing. Recently, activated platelet rich plasma (PRP) has been widely used in the field of bone tissue engineering, since it harb...

Low frequency electric fields were exposed to various water samples using platinum electrodes mounted near the water surface. Responses were monitored using a spectro-radiometer and a contact-angle goniometer. Treatment of DI (deionized), EZ (Exclusion Zone), and bulk water with certain electromagnetic frequencies resulted in a drop of radiance per...

The recent viral infection disease pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in a global public health crisis. Iran, as one of the countries that reported over five million infected cases by September 2021, has been concerned with the urgent development of effective vaccines against SARS-CoV-2. I...

Mechanism of magnetic fluid hyperthermia (MFH) studied via histological, cellular and in-silico methods. Number of RGB pixels in histopathological or cellar images was calculated to estimate penetration ratio of magnetic nanocomposite (MNC; MoS2/CoFe2O4) into either of tumor tissue or cells. The hydrodynamic diameter and the size distribution of MN...

Multiple Sclerosis (MS) is the most prevalent neurological disability in young adults. The pathogenesis of MS is characterized by demyelination and neurodegeneration in the central nervous system (CNS) as the ruinous result of chronic activation of the immune system. All clinical forms of MS, including relapsing-remitting multiple sclerosis (RRMS),...

Background: Glioblastoma multiforme (GBM) is the most common primary malignat brain tumor in adults. The modulation of miRNA expression is considered both as controlling groundwork for cancer development and invasion and as a potential application in GBM-targeted therapies either perse or combined with chemo-or radiotherapy. The c-myc overexpressio...

The aims were to characterize MoS2/CoFe2O4 nanocomposite and investigate its thermal therapy efficiency on cancerous (MCF-7) and normal (MCF-10A) human breast cells. Magnetic nanocomposites (MNCs) characterized by scanning/transmission electron microscopy (SEM/TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and vibrati...

Application of hyperosmolarity can be a promising strategy to promote chondrogenic differentiation in adipose-derived mesenchymal stem cells (ADSCs). Growth factors may promote different signaling pathways in parallel that is why in this study we monitor undesired pathologic or unwanted side effects as well as chondroinductive impacts of hyperosmol...

In this study, antibacterial impact of magnetic nanocomposite (MNC: MoS2/CoFe2O4) and super paramagnetic iron oxide nanoparticle (SPION: Fe3O4-Casein) against staphylococcus aureus was studied. It was expected that such comparative study, address key points in optimization of synthesis and design of nanomaterials, which are supposed to be used as a...

Protein aggregation is known as the main mechanism of amyloid fibrillation in amyloidosis diseases. Recent studies confirmed that compounds with one or two indole rings have inhibitory potential against amyloid fibrillation. Herein, the interaction of two similar compounds ‘bis(indolyl)-2-methyl-phenyl-methene’ and ‘bis(indolyl)-2-chloro-phenyl-met...

Cleavage factor “CFIm25”, as a key repressor at proximal poly (A) site, negatively correlates to cell proliferation and tumorigenicity in various cancers. Hence, understanding CFIm25 mechanism of action in breast cancer would be a great benefit. To this aim four steps were designed. First, potential miRNAs that target 3′-UTR of CFIm25 mRNA, retriev...

Logistic complexities of heart transplantation embossed the necessity of utilizing novel methods, which enable heart regeneration. Human cardiosphere-derived cells (hCDCs) are taken into consideration as a promising cell resource in cell therapy in recent years. In this study, we designed an electrochemical stimulation system, which sends square pu...

Background: There are different methods to develop in vitro neo-chondral tissues from adipose-derived stem cells (ADSCs). Application of electromagnetic field (EMF) on ADSCs is one of popular approaches, which results in chondrogenesis. If chondrogenic impact of EMF on ADSCs is supposed to be generalized as a protocol in translational medicine fiel...

Mimicking the structure of extracellular matrix (ECM) of myocardium is necessary for fabrication of functional cardiac tissue. The superparamagnetic iron oxide nanoparticles (SPIONs, Fe3O4), as new generation of magnetic nanoparticles (NPs), are highly intended in biomedical studies. Here, SPION NPs (1 wt%) were synthesized and incorporated into si...

span style="color: #1f497d;">Introduction: Transforming growth factor-beta (TGF-β) is known as standard chondrogenic differentiation agent, even though it comes with undesirable side effects such as early hypertrophic maturation, mineralization, and secretion of inflammatory/angiogenic factors. On the other hand, platelet-rich plasma (PRP) is found...

Diarrheal diseases represent a major health problem in developing countries. Several viruses and bacterial agents, such as Enterotoxigenic Escherichia coli (ETEC) and Enterohemorrhagic Escherichia coli (EHEC) are responsible for human enteric infections. In humans, EHEC infections result in bloody or non-bloody diarrhea, which may be complicated by...

Lipid-binding domains regulate positioning of the membrane proteins via specific interactions with phospholipid’s head groups. Spinal cord injury (SCI) diminishes the integrity of neural fiber membranes at nanoscopic level. In cases that the ruptured zone size is beyond the natural resealing ability, there is a need for reinforcing factors such as...

Objectives: The aim of the current study was to mimic mechanical impacts on the spinal cord by manifesting the effects of dorsoventral (DVMP) and lateral (LMP) mechanical pressure on neural activity to address points to be considered during surgery for different purposes, including spinal cord decompression. Approaches: Spinal cords of anestheti...

Membrane sealing effects of polymersomes made of tri-block copolymer, PEG-co-FA/SC-co-PEG, (PFSP) were studied on isolated spinal cord strips, PC12 cell lines and artificial bilayer following mechanical impact implemented by aneurism clip, sonication and electric shock, respectively. The homogeneity and size of PFSP, membrane permeability and cell...

Stimulated plasticity in spinal cord has been considered as a therapeutic method for SCI based on both experimental and computational studies. Synapse plasticity is highly related to the ionic and resulted electric current across the neuron membrane. The current produces an evanescent electric field that may affect other neurons at a fixed distance...

Abstract: Stimulated plasticity in spinal cord has been considered as a therapeutic method for SCI based on both experimental and computational studies. Synapse plasticity is highly related to the ionic and resulted electric current across the neuron membrane. The current produces an evanescent electric field that may affect other neurons at a fixe...

Immediate membrane sealing after spinal cord injury (SCI) can prevent further degradation and result in ultimate functional recovery. It has been reported that polyethylene glycol (PEG) can repair membrane damage caused by mechanical insults to the spinal cord. Furthermore, membrane fluidity and its sealing process vary at different temperatures. H...

Membrane disruption is one of the main factors that cause axonal damage and functional deficits manifested in spinal cord injury (SCI). In this study, we used polyethylene glycol (PEG) to induce immediate membrane sealing and to promote functional recovery after SCI. The effects of PEG (200-2000 Da) on the damaged membrane were monitored by means o...

Compound action potential (CAP) of spinal cord represents valuable properties of neural fibers including excitability, rate of myelination and membrane integrity. These properties are measured using amplitude, latency and area under curve of CAPs recorded from spinal cord. Here, the isolated spinal cord was set in a double sucrose gap (DSG) chamber...

Mechanical damages cause great pressure on membranes that lead to partial or complete disruption of membrane and have to be manifested at nano-scale level. Polyethylene glycol (PEG) is a well-studied non-toxic, hydrophilic polymer used for cell membrane repair. Different forms of PEG have been administrated intravenously in animal models or incubat...

Introduction : In-vivo application of tri-block copolymer after secondary stage of spinal cord injury (SCI) re-established integrity, rapid recovery of nerve impulse conduction in neurons mediating the long tract reflexes. Rupture of neuronal membranes following spinal cord injury, causes Ca2+ influx into cells in primary stage. Consequent biologic...

Many proteins contain domains (e.g. PH superfamily domains, FYVE domains, C2 domains, ENTH domains and etc.) that interact with membranes and regions that can interact with cytoskeletal components. Some of these proteins are important regulators of membrane-cytoskeleton interactions according to recognition of soluble headgroups of their lipid lig...

Rabies is acutely fatal encephalitis caused by a neurotropic virus. This virus belongs to the family of Rhabdoviridae and genus of Lyssavirus. The virus is almost always transmitted to human through infected mammalian saliva. Rabies is inoculated to a wound by an infected animal bite. Since infection is established in the CNS, the outcome is almost...

In this work, Pseudomonas sp. SA01 cells were immobilized in a series of singular and hybrid immobilization techniques to achieve enhanced phenol removal. The singular immobilization strategies consisted of various concentrations of alginate (2-4%) and pectin (3-5%), while the hybrid strategies incorporated polyvinyl alcohol (PVA)-alginate and glyc...

Using the phenol enrichment cultivation method, we succeeded in screening a few bacterial strains with a varied ability for utilizing phenol as the sole carbon source from pharmaceutical disposal wastewaters plant. Among these strains, a potent phenol-degrading bacterium, assigned Pseudomonas sp. SA01, was identified and examined as having the grea...

Pseudomonas strains are known as one of the most abundant microorganisms that make physiological response to phenolic pollutant, due to their loci that are located on plasmid or chromosome. Regarding this fact that almost all of monocyclic aromatics convert to different derivatives of catechol, catechol 2, 3-dioxygenase is the key enzyme in the met...

Pseudomonas strains are known as one of the most abundant microorganisms that make physiological response to phenolic pollutant, due to their loci that are located on plasmid or chromosome. Regarding this fact that almost all of monocyclic aromatics convert to different derivatives of catechol, catechol 2, 3-dioxygenase is the key enzyme in the met...

In the present study, four carbon sources were tested for the polyester synthesis of Pseudomonas aeruginosa PA01 which is a ubiquitous environmental bacterium and is one of the top three causes of opportunistic human infections. These included linear C 8 to C 11 carboxylic acids. Octanoic acid, nonanoic acid, decanoic acid and undecanoic acid were...

To find the prevalence of domestic and wild animal bites in general, and that of rabies disease in human, in particular in Kerman province, southeastern Iran, a retrospective study was conducted. The necessary data including residence place (urban or rural) of bitten persons, age and job of people were collected during a 10-year period from 21 Marc...