Ilhan Yaylim

• professor
• Professor at Istanbul University

Non-small cell lung cancer (NSCLC) is characterized by a complex and heterogeneous molecular basis. Telomerase reverse transcriptase (TERT) gene promoter mutations have been implicated in various cancer types. We aimed to investigate the status of TERT promoter region mutations in NSCLCs and determine associations of clinicopathological connections...

Introduction: Gastric cancers (GC) are one of the main causes of cancer-related deaths worldwide. Despite the dizzying progress in diagnostic and therapeutic strategies, many GC cases are diagnosed at advanced stages. Various signaling pathways have been identified to play vital roles in GC. Although the importance of these signaling pathways in GC...

Introduction The most prevalent head and neck cancer type is laryngeal cancer. Laryngeal cancer susceptibility is increased by a combination of genetic variables and environmental factors. Genetic predispositions that influence the functioning of the immune system can affect tumor development. Our study investigates the impact of alterations in CD4...

The AgNPs‐Rsvl complex was investigated for its anti‐cancer effects on human breast cancer cell lines. The UV–vis spectrophotometer was used to perform synthesized AgNPs‐Rsvl binding analyses. The size and morphology of the AgNPs were determined by field emission gun‐transmission electron microscopy (FEG‐TEM). The surface chemical signatures of AgN...

Cytotoxic T-lymphocyte associated protein 4 (CTLA-4) was the first immune checkpoint molecule to be used as a drug target and led the way in the field of immunooncology. CTLA-4 increases the activation threshold of T-cells and reduces immune responses to weak antigens, such as self and tumour antigens. In our study, 56 patients were diagnosed with...

Oxidative stress is characterized by the deregulation of the redox state in the cells, which plays a role in the initiation of various types of cancers. The activity of galectin-1 (Gal-1) depends on the cell redox state and the redox state of the microenvironment. Gal-1 expression has been related to many different tumor types, as it plays importan...

Obesity is an important healthcare issue caused by abnormally increased adipose tissue because of energy-intake overcoming energy expenditure. Disturbances in the physiological function of adipose tissue mediate the development of diabetes. It is a metabolic disease that results from decreased insulin-levels and/or changes in the insulin action mec...

Objectives:Recent studies have shown that the distribution of the tryptophan/kynurenine pathway (KP) plays a role in the development of Obsessive Compulsive Disorder (OCD). We aimed to reveal the relationship between CYP1A1 rs464903 and AhR rs10249788 associated with the KP and Interferon gamma (IFN γ) and oxidative stress in OCD. Methods:In our st...

The aim of this study was to investigate a possible association between the variations and effects of gene changes in the OX40/OX40L pathway and the risk of developing bladder cancer in a Turkish population. The study included 104 patients with bladder cancer and 97 healthy individuals. The distribution of OX40 (rs17568) and OX40L (rs1234313) polym...

TRAIL, a member of the TNF family, is expressed in tumor and tumor surrounding tissue in many solid organ cancers. While the induction of tumor-specific apoptosis in correlation with cytokine stimulation may cause anti-tumoral effects, the pro-tumorigenic effects of its expression by tumor surrounding tissue members have been reported in the litera...

Background/aim: MicroRNAs (miRNA) are a class of small non-coding RNAs of 18-25 nucleotides, which regulate gene expression at the post-transcriptional level by disrupting or blocking translation of messenger RNA targets. Non-small cell lung cancer (NSCLC) represents approximately 85% of all lung cancers. Early and accurate diagnosis of the diseas...

Background/aim: Colorectal cancer (CRC) is one of leading cancers in terms of incidence and mortality. Interaction of tumor cells with the surrounding microenvironment plays a crucial role in the development and progression of CRC. Many pathways such as the kynurenine pathway, OX40/OX40L-mediated signaling and microRNAs targeting PD-L1 may be invo...

Background Obesity is an important healthcare issue caused by abnormally increased adipose tissue because of energy-intake overcoming energy expenditure. Disturbances in the physiological function of adipose tissue mediate the development of diabetes. It is a metabolic disease that results from decreased insulin-levels and/or changes in the insulin...

Background/aim: Oral squamous cell carcinoma (OSCC) is a malign tumor that associated with smoking and alcohol consumption, eating habits, environmental factors, and genetic susceptibility of the individuals. The Survivin gene, also known as BIRC5, plays important roles in the regulation of the cell cycle and apoptosis. The aim of the present stud...

Meme kanseri, kadınlarda en yaygın görülen kanserlerden biridir ve dünyadaki en yüksek insidansa sahip kanser türüdür. Meme kanseri gelişiminde cinsiyet, yaş, östrojen düzeyi, aile öyküsü, gen mutasyon- ları ve sağlıksız yaşam tarzı gibi çok sayıda risk faktörü bulunmaktadır. Mevcut tedavi seçenekleri cerrahi, radyoterapi, hormonal tedaviler, kemot...

Objectives Apoptosis is defined as programmed cell death, which regulates cellular functions and various physiological responses. Several studies reported that Caspase genes play important roles in the apoptosis and inflammation process. Caspase-8 (CASP8) is a member of the cysteine protease family and a key regulator gene in the induction of apopt...

NORAD, non-coding RNA activated by DNA damage, is a Long non-coding RNA (lncRNA) transcript that modulates genome stability and has been reported to be dysregulated in different cancers. Although it has been reported to be upregulated in tumor cells mostly for solid organ cancers, it has also been reported to be downregulated in some cancers. Altho...

Background/aim: Mitogen-activated protein kinases (MAPKs) are important regulatory molecules, which have essential roles in physiology and pathology. In the present study, we examined the possible correlation between the MAPK7 gene and colorectal cancer risk in the Turkish population. Materials and methods: A total of 100 human DNA samples (50 c...

Background and objective: Globally, gastric carcinoma (Gca) ranks fifth in terms of incidence and third in terms of mortality. Higher serum tumor markers (TMs) than those from healthy individuals, led to TMs clinical application as diagnostic biomarkers for Gca. Actually, there is no accurate blood test to diagnose Gca. Methods: Raman spectrosco...

Colorectal cancer is the second most common cause of cancer-related deaths worldwide. To follow up on the progression of the disease, tumor markers are commonly used. Here, we report serum analysis based on Raman spectroscopy to provide a rapid cancer diagnosis with tumor markers and two new cell adhesion molecules measured using the ELİSA method....

Background The bladder cancer (BC) pathology is caused by both exogenous environmental and endogenous molecular factors. Several genes have been implicated, but the molecular pathogenesis of BC and its subtypes remains debatable. The bioinformatic analysis evaluates high numbers of proteins in a single study, increasing the opportunity to identify...

Background 1,25(OH)2D3(Calcitriol), which is a broad regulatory molecule, plays a role in changing the efficacy of chemotherapeutic drugs. Cisplatin is one of a current standard chemotherapy regimen for bladder cancer. Increasing the effectiveness of the treatment and reducing the side effects to chemotherapeutics are of great importance in bladder...

This study explores the machine learning-based assessment of predisposition to colorectal cancer based on single nucleotide polymorphisms (SNP). Such a computational approach may be used as a risk indicator and an auxiliary diagnosis method that complements the traditional methods such as biopsy and CT scan. Moreover, it may be used to develop a lo...

Background and Aim: Chronic hepatitis B virus (HBV) infection is a major cause of hepatocellular carcinoma (HCC). Circulating cell-free DNA (cfDNA) methylation of tumor suppressor genes are emerging potential biomarkers in HCC. We aimed to evaluate the cfDNA methylation status of RASSF1 and CDKN2AIP genes in patients with liver cirrhosis (LC) with...

Biyokimya, canlı sistemde gerçekleşen kimyasal olayları, bu sistemde yer alan tüm moleküllerin yapısını, bileşimini ve fonksiyonunu inceleyen bir bilim dalıdır. Temel biyokimya, biyokimyasal mekanizmaların moleküler düzeyde anlaşılması için alt yapıyı oluştururken hastalıklar ve tedavilerin öğrenilmesini kolaylaştırmaktadır. Biyokimyasal olayların...

Objective/Aim Endometrisosis, one of the most common gynecological disease, is characterized by the presence of endometriotic tissue outside of uterine cavity. The development and the validation of a simple blood biomarker specific and sensitive for endometriosis may facilitate the rapid and the accurate diagnosis of the disease and thus early trea...

The human ribosomes are the cellular machines that participate in protein synthesis, which is deeply affected during cancer transformation by different oncoproteins and is shown to provide cancer cell proliferation and therefore biomass. Cancer diseases are associated with an increase in ribosome biogenesis and mutation of ribosomal proteins. The r...

Laryngeal tumours with multifactorial etiopathogenesis constitute ∼1% of all body cancers. The imbalance between oxidative and antioxidative systems affects redox-homeostasis. The oxidative stress generated by the continuous formation of reactive oxygen species results in the oxidation of cellular molecules. The present study investigated the prote...

Kan glukozunun yükselmesiyle karakterize olan diyabet, pankreastaki fonksiyon bozukluğu neticesinde oluşmaktadır ve bu durum insülin etkisine karşı dirence veya insülin üretiminin azalmasına neden olmaktadır. Diyabet, Tip-I ve Tip-II olmak üzere iki grupta incelenmektedir. Tip-I diyabet, pankreasın beta hücresinden salınan insülin hormonundaki yete...

Background: Intercellular adhesion molecule-1 (ICAM-1) is a surface glycoprotein important for tumor invasion and angiogenesis. The present research is conducted to investigate whether specific gene polymorphism of ICAM-1 K469E (rs5498) and plasma redox status could be associated with laryngeal cancer (LC) development. Since there is no clear evide...

Cancer is a multifactorial disease and a wealth of information has enabled us to develop a better understanding of the heterogeneous nature of cancer. Deregulation of spatio-temporally controlled signaling cascades played a central role in cancer progression. NRF2-driven signaling has attracted significant attention because of its ability to dualis...

Tryptophan metabolism in the tumor microenvironment exerts immunosuppressive effects by affecting the anti-tumor functions of immune cells. The immunosuppressive roles of tryptophan and tryptophan metabolites and their effects on the FOXP3 gene, highly expressed in regulatory T cells (Tregs), are remarkable. Our study aimed to investigate the relat...

Background/aim: Functional and bioinformatic studies provide strong evidence that long non-coding RNAs (lncRNAs) can alter the molecular mechanisms of cancer through their interactions with DNA, RNAs, and proteins. This study aimed to evaluate the role of H19 and LINC00675 lncRNAs in colorectal cancers (CRCs) in terms of clinicopathological featur...

This study was aimed to investigate the effect of weight loss by bariatric surgery on the level of anti-Mullerian hormone (AMH) in morbidly obese female patients with or without polycystic ovary syndrome (PCOS). This prospective study includes 70 females, obese, and fertile patients of reproductive age. All patients were evaluated to determine the...

Introduction Although potential relationships with genetic variants of MLCK2, AMPD1 and COL5A1 have been detected in molecular studies evaluating sports performance from the genetic perspective, there are limited data in terms of muscle endurance and physical fitness. Materials and Methods This study aimed to evaluate these variants in terms of lo...

1,25(OH)2D3 (Calcitriol, active form of Vitamin D), which is a broad regulatory molecule, plays a role in changing the efficacy of chemotherapeutic drugs. In bladder cancer, effectiveness of the treatment and reducing the resistance to chemotherapeutics such as cisplatin are of great importance. Cisplatin usage is one of a current standard chemothe...

Recent breakthroughs in genomics and proteomics have fostered a renaissance of seminal research works on metastasis, leading to a conceptual framework for a mechanistic understanding of the regulatory role of SMURFs in carcinogenesis and metastasis. SMURFs have gained considerable limelight because of their hallmark ability to target myriad of prot...

Rapidly growing body of multiomics data has enabled us to develop a better understanding of the heterogeneous nature of cancer. There is a growing momentum behind the application of this knowledge to pharmaceutically target oncogenic cell signaling pathways to inhibit and/or prevent carcinogenesis and metastasis. Circular RNAs have gained extraordi...

Objectives The PI3K (Phosphatidylinositol 3-kinase) is the member of lipid kinase family that plays important roles in tumorigenesis, cancer development and cell proliferation. In our study, we aimed to investigate the relationships between breast cancer risk and prognosis with PIK3CA rs6443624 (C>A) intron region gene polymorphism and serum PI3K l...

Background: Mesenchymal stem cells (MSCs) have gained remarkable attention because of their ability to dualistically regulate tumor growth. The main objective of this study was to evaluate the apoptotic effects of human bone marrow-derived (hBM) MSCs in combination with interferon gamma (IFN-γ) on MCF-7 breast cancer cells, and to determine the cy...

Iron(III) and nickel(II) complexes bearing a thiosemicarbazone framework were synthesized by a one‐pot synthesis method. The structures were characterized by elemental analysis, IR, 1H NMR, APCI Mass, conductivity, magnetic moment measurements. Molecular and crystal structures of the iron(III) complex were obtained from single‐crystal X‐ray diffrac...

Introduction: The data suggesting that metabolites and genes of homocysteine may play a role in the pathogenesis of colorectal cancer are available in the literature. Similarly, selenium (Se) levels and SELENBP1, a member of the selenoprotein family characterized by selenium, have been evaluated for the etiopathology of colorectal cancer (CRC). In...

Blueberries belong to the genus Vaccinium of the family Ericaceae. Rapidly accumulating experimentally verified data is uncovering tremendous pharmacological properties of biologically active constituents of Blueberries against different diseases. Our rapidly evolving knowledge about multifaceted nature of cancer has opened new horizons to search f...

Due to many biological cell functions of vitamin D including regulation of cell survival, proliferation and differentiation, the metabolism of itself gains importance in the development of several types of cancer. This case–control study was designed to evaluate the risk of gastric cancer development in terms of VDR rs2228570 & rs731236, and VDBP r...

Inula viscosa is a type of Asteraceae family, which widely used for medicinal plant to treatment of different diseases. Inula viscosa contains many biological compounds, such as monoterpenes, sesquiterpenes, diterpenes, flavonoids. Our study aimed to investigate the cytotoxic effects of Inula viscosa extract on MCF-7 (breast carcinoma), C6 (glioblo...

Thiosemicarbazones are an important subject of medicinal drugs. In this study, mixed-ligand thiosemicarbazone complexes were synthesized and the cytotoxic activities were ivestigated in vitro breast cancer cells.

This study aimed to evaluate antiproliferative and proapoptotic effects of Capecitabine bonded silver particles on human breast cancer cells (MCF-7). Different sizes of Ag NPs (in sizes 5, 10, 15, 30 nm) were synthesized. The characterization of silver and drug-bonded silver nanoparticles was performed through UV-VIS, FTIR, and SEM analysis. Silver...

Introduction: Although the etiology of congenital heart disease is uncertain, interaction of genetic and environmental factors has been implicated in 90% of cases. The purpose of this study was to investigate the relationships between serum zinc, copper, and selenium levels and congenital heart disease. Materials and methods: Zinc, copper, and sele...

Background: The purpose of this study was to investigate the phenolic composition and antioxidant activity of Thymbra sintenisii subsp. isaurica extract (TSIE) and, to evaluate, for the first time, anticancer effect on human MCF-7 (breast carcinoma) cells. Materials and Methods: The antioxidant capacity of TSIE was evaluated using 2,2-diphenyl-1-pi...

Background: The purpose of this study was to investigate the phenolic composition and antioxidant activity of Thymbra sintenisii subsp. isaurica extract (TSIE) and, to evaluate, for the first time, anticancer effect on human MCF-7 (breast carcinoma) cells. Materials and Methods: The antioxidant capacity of TSIE was evaluated using 2,2-diphenyl-1-pi...

Rapidly accumulating preclinical and clinical studies have helped us to unveil underlying mechanisms of colorectal cancer development and progression. Deregulated signaling pathways play instrumental role in carcinogenesis, drug resistance and metastasis. Wnt signaling cascade has attracted considerable attention in colorectal cancer as many ground...

Large-scale sequencing methodologies have helped us identify numerous genomic alterations and we have started to scratch the surface of many new targets for treatment of cancer and the associated predictive biomarkers. TRAIL (TNF-related apoptosis-inducing ligand) is a highly appreciated anti-cancer molecule because of its ability to selectively ta...

Based on the exciting insights gleaned from decades of ground-breaking research, it has become evident that deregulated signaling pathways play instrumental role in cancer development and progression. Interestingly discovery of non-coding RNAs has revolutionized our understanding related to transcription, post-transcription and translation. Modern...

It is known that disorders in apoptosis function play an important role in the pathogenesis of many types of cancer, including lung cancer. Tumor necrosis factor related apoptosis inducing ligand (TRAIL), a type II transmembrane protein, is a death ligand capable of inducing apoptosis by activating distinctive death receptor. Our purpose in this st...

Background/aim: The single nucleotide polymorphism -31C/G identified in the survivin gene promoter seems to be associated with over-expression of survivin, an anti-apoptotic protein. In gliomas, increased survivin expression correlated with decreased survival. The aim of the study was to investigate whether survivin gene polymorphism associates wi...

Piperlongumine is a biologically and pharmacologically active constituent of the plant Piper longum. This compound is gradually gaining attention because of its ability to inhibit/prevent different cancers. Modern era of molecular oncology is incomplete without ground-breaking discoveries made in the field of cell signaling pathways. High-throughpu...

Recent technological and analytical breakthroughs in genomics and proteomics have deepened our understanding related to the multifaceted nature of cancer. Because of therapeutically challenging nature of cancer, there has been a renewed interest in phytochemistry, and much attention is currently being given to the identification of signaling pathwa...

PD-L1, which is a member of the B7 family co-inhibitory molecules and the expression level of PD-L1 on colorectal cancer,plays a crucial role in tumour immune escape via impaired cytokine production.PDL1/PD1 polymorphisms were investigated in 181 subjects (87 subjects with CRC and 94 healthy individuals as controls) by using polymerase chain reacti...

Objectives: Intercellular adhesion molecule-1 (ICAM-1) plays an important role in endothelial function. Hyperglycemia-induced impaired redox status is 1 of the well-known pathophysiologic characteristics of gestational diabetes mellitus (GDM), and it plays a crucial role in the causes of disease. Our aim was to clarify any possible relationship bet...

Despite being the most common chronic liver disease, the pathogenesis of nonalcoholic fatty liver disease (NAFLD) still remains unclear. According to the genome-wide association studies (GWAS) alternative alleles of the farnesyl-diphosphate farnesyltransferase 1 (FDFT1) gene involved in cholesterol biosynthetic pathway are known to affect hepatic s...

Research over decades has progressively explored pharmacological actions of bitter gourd (Momordica charantia). Biologically and pharmacologically active molecules isolated from M. charantia have shown significant anti-cancer activity in cancer cell lines and xenografted mice. In this review spotlight was set on the bioactive compounds isolated fro...

Introduction Introduction: Programmed death-1 (PD-1), an important immunosuppressive molecule, plays a key role in tumor-cell-mediated immune escape. The present study aimed to investigate the role of PD-1.5 (C/T) gene polymorphisms on the susceptibility and progression of colorectal cancer (CRC). Material and methods In this study, the PD-1.5 C/T...

Researchers have developed a better understanding of deregulation of spatio-temporally controlled signaling cascades which contribute in cancer development and progression. The greatest stumbling block in improving the clinical outcome of cancer patients is that tumors continuously adapt to their micro-environment and evolve ways to develop resista...

Aim: P16, which is localized on chromosome 9p21, is an important tumor suppressor protein of 156 amino acids. The 3'UTR region polymorphisms of the encoding gene has been demonstrated to be related with cancer development and prognosis. MDM2 is an important protein that plays a crucial role in stopping cell cycle at G1/S transition of p53 and preve...

This work reports on the synthesis and characterization of phthalocyanines (M = Cu(II) (2), Zn(II) (3) In(III) (4) and Co(II) (5)) peripherally tetra-substituted with 1-(4-hydroxyphenyl)propan-1-one. Confirmation of the synthesized phthalocyanine structures are performed with a combination of elemental analysis, FTIR, ¹H-NMR, ¹³C-NMR, UV-vis and MA...

Laryngeal squamous cell carcinoma (LSCC) is a genomically complex disease that is difficult to target, and efforts have been made to identify new treatment strategies and molecular markers that might stratify patients and individualize options for treatment. miR-373 has diametrically opposed roles in different stages and types of cancers. miR-373 h...

Investigation of PD-1 gene polymorphism (PD-1.5 C/T) in turkish patients with laryngeal cancer Bayram Kıran1, Şeyda Ercan2, Yosra Lamami3, Dilara Sönmez2, Mehmet Tolgahan Hakan2, Ayşegül Verim4, İlhan Yaylım2 1Genetic and Bioengineering Department, Faculty of Science,Kastamonu University, Kastamonu, Turkey 2Molecular Medicine Department, Aziz Sanca...

Oxidative status is attributed to endothelial dysfunction and might be one of the key mechanisms of endothelial dysfunction in acromegaly. In this study, we aimed to investigate the effect of acromegaly on superoxide dismutase (SOD) and total antioxidant capacity (TAC) levels, and the possible influence of human manganese superoxide dismutase (MnSO...

Objective Gastric cancer is one of the most common malignancies worldwide. The risk factors for gastric cancer include environmental and genetic factors. Inflammation and the immune system are known to contribute to the development of the gastric cancer. We examined the influence of critical polymorphisms of CTLA-4 and CD28 genes and circulating pr...

Data obtained from Human Genome Project has helped in transition of human diseases from a segmented view to a conceptual continuum. In accordance with this approach, identification of new gene targets has reinvigorated the field of natural product research and predominantly scientists are working to obtain these drugs through the use of high-throug...

Aim: Prolactinomas are thought to arise from clonal expansion of a single mutated cell which is subjected to growth stimuli of several permissive factors, although the pathogenetic mechanisms underlying tumorigenesis remain unclear. The present study aimed to investigate the role of p16 (540C→G and 580C→T) and mouse double minute 2 (MDM2) (SNP309T...

Kanser; hücrelerin kontrolsüz bir şekilde çoğalması ve apoptoz yeteneğini kaybetmesi sonucu oluşan patolojik bir durumdur (1). Kanser dünyadaki pek çok ülkede hastalık kaynaklı ölümlerin başlıca ikinci nedenidir. Kanser insidansının bu kadar yüksek olmasının nedenleri arasında yaş ortalaması, beslenme alışkanlığı, çevresel karsinojenlere maruz kalm...

Overwhelmingly increasing scientific evidence has provided near complete resolution of prostate cancer landscape and it is now more understandable that wide ranging factors underlies its development and progression. Increasingly it is being realized that genetic/epigenetic factors, Intra-tumoral and inter-tumoral heterogeneity, loss of apoptosis, d...

Prostate cancer is a genomically complex disease and data obtained through in-vitro, preclinical and clinical researches has significantly improved our understanding of the dysregulations of spatio-temporally controlled signaling cascades. Using high-throughput technologies, it has been shown that signaling cascades are re-wired in androgen depende...

Increasingly it is being realized that oral cancer arises from genetic/epigenetic mutations, dysregulations of spatio-temporally controlled signal transduction cascades and loss of apoptosis. Epidemiological studies have provided a stronger association between tobacco use (chewed and smoked) and oral cancer. Nevertheless, alcohol has also gained at...

In 1960 researchers reported that balanced translocation between chromosomes 22 and 9 resulted in the generation of Philadelphia chromosome. This breakthrough revolutionized our knowledge related to leukemia biology and contemporary studies revealed that chromosomal translocation resulted in the fusion between the 5' segment of BCR gene and 3' segm...

This study aimed to investigate antioxidant and anti-inflammatory activities of a commercial product of noni (Morinda citrifolia) juice. Carrageenan-induced rat paw edema was employed as inflammatory model. One control and three experimental groups were formed. Experimental groups were administered noni juice alone, noni juice+carrageenan, and carr...