Ilgaz Akata

Ankara University · Department of Biology

Background: Mushrooms are shown to protect against the side effects of cancer. Therefore, mushrooms with proven anticancer properties and active ingredients are fascinating in the search for new cancer drugs. Objective: In this study, the effects of extracts from Hericium coralloides (M1), Lactarius deliciosus (M2), Lepista nuda (M3), Pleurotus os...

Citation: Ozsari, S.; Kumru, E.; Ekinci, F.; Akata, I.; Guzel, M.S.; Acici, K.; Ozcan, E.; Asuroglu, T. Deep Learning-Based Classification of Macrofungi: Comparative Analysis of Advanced Models for Accurate Fungi Identification. Sensors 2024, 24, 7189. Abstract: This study focuses on the classification of six different macrofungi species using adva...

The connections between viruses and their hosts are complex and can arise from any combination of different evolutionary events including “codivergence”, “switching”, and “duplication” of the pathogen. Mycoviruses, a diverse virus group whose members specifically infect fungal hosts, are subject to the similar evolutionary processes. In this study,...

Studying the diversity of viruses found in uncultivated fungi, including those forming mycorrhizal relationships, is essential. It’s equally important to explore viral communities in fungi that cause plant diseases or are economically significant. This dual approach helps us grasp the full scope of mycovirus diversity and evolution. Mycorrhizal fun...

In 2023, specimens of Rhodocybe asyae were collected from the Tınaztepe Campus of Dokuz Eylül University (İzmir-Türkiye). These samples underwent a comprehensive and meticulous evaluation involving morphological examination and phylogenetic analysis based on nrITS rDNA sequencing. The DEU AKATA & SAHIN 148 samples exhibited microscopic and macrosco...

Aim of study: The primary aim of this research is to contribute Turkish mycobiota by adding ten rust fungi species that have not been previously reported in the country. Area of study: Aladağlar and Bolkar Mountains are part of the broader Central Taurus Mountains complex situated in the eastern section of southern Anatolia. It is encircled by seve...

Aim of study: The main aim of this study is to contribute to the fungal diversity of Türkiye by presenting two newly documented species within the order of Agaricales. Area of study: The samples of the newly reported species were collected under pine trees in Ankara Üniversitesi Beşevler 10. Yıl Campus, Ankara, and in fir forest at At Plateau, loca...

The cytotoxic activities of apolar extract of Lactarius salmonicolor and its fractions and subfractions were screened against colon (CaCo-2), prostate (LNCaP), lung (H1299), and breast (MCF-7) cancerous cell lines. The main extract of Lactarius salmonicolor that was cytotoxic to CaCo-2 (EC50: 137.1 ± 2.7 mg/mL) and LNCap (EC50: 131.2 ± 2.6 mg/mL) m...

Inocybe specimens were collected from Ankara University Beşevler 10. Yıl Campus, (Ankara, Türkiye) on October 19, 2022. As a result, the samples were identified as I. costinitii, a new record for Turkish Inocybe. This study presents a detailed description of this newly recorded species, covering aspects such as its location, observations of its hab...

The extensive use of high-throughput sequencing (HTS) has significantly advanced and transformed our comprehension of virus diversity, especially in intricate settings like soil and biological specimens. In this study, we delved into mycovirus sequence surveys within mycorrhizal fungus species Terfezia claveryi, through employing HTS with total dou...

Advancements in high-throughput sequencing and the development of new bioinformatics tools for large-scale data analysis play a crucial role in uncovering virus diversity and enhancing our understanding of virus evolution. The discovery of the ormycovirus clades, a group of RNA viruses that are phylogenetically distinct from all known Riboviria mem...

In the present study, fungal specimens collected from Yeşildere village in Uşak province, Türkiye were evaluated. The specimens underwent detailed examination through both morphological and DNA (nrITS rDNA region and TEF1a gene) sequence-based phylo-genetic analysis. Based on the evaluation of micro-and macromorphological characteristics, and phylo...

Studies continue every day with the increase in multi-drug resistance in bacteria. In this study, the effect of potential inhibitors against the efflux pump with natural components in extracts obtained from some macrofungus to increase the effectiveness of antibiotics was investigated with an in silico study. Molecular docking was performed on the...

Members of the genus Armillaria belong to the group of pathogenic and facultative saprotrophic fungi that are generally known as one of the causative agents of white root rot in infected plants including deciduous and evergreen trees and shrubs. Although several single-stranded RNA mycoviruses were previously described in different Armillaria speci...

Tricholoma anatolicum is an edible mushroom from the matsutake group growing under Cedar trees. Bioactivity-guided fractionation of Tricholoma anatolicum afforded two new (1 and 2), three known ergosterols (3-6), and four known (6-9) compounds. Structures were identified as anatoluin A (1), anatoluin B (2), 5α,6α-epoxy-ergosta-7,22-dien,3β-ol (3),...

Antibiotic resistance, a growing concern for various reasons, underscores the urgency of discovering new antibiotics. However, the emergence of resistance mechanisms remains a formidable challenge. One such mechanism is the efflux pumps, which actively extrude antibiotics from bacterial cells, reducing their intracellular concentrations. Inhibiting...

Rust species Milesina scolopendrii on Asplenium scolopendrium and Triphragmium filipendulae on Filipendula vulgaris are reported from Melsin, Turkey. These are new generic records from Turkey. The morphological characteristics are described and illustrated based on the collected materials.

In March and December of 2022, fungal samples (AKATA&SEN TT 001, AKATA TT 335, and AKATA TT 403) were collected from Enez and Süloğlu provinces of Edirne, Türkiye. The samples were gathered using truffle-detecting dogs, and their macroscopic characteristics and ecological attributes were documented in their natural habitats. These samples were anal...

Antibiotic resistance has become the biggest problem of the 21st century. It is estimated that this problem will result in around 10 million deaths per year by 2050. Macrofungi are very important for the discovery of new antibiotics as natural sources with potential microbial effects. In this study, the effect of the ethanol extract of P. hartigii...

Aim of the study: The principal objective of this study is to contribute to the Turkish mycobiota by including three newly reported agaricoid macrofungi species. Study area: Situated approximately 5 km from the city center in the Beşevler area of the Çankaya district in Ankara, Ankara University's Beşevler 10th Year Campus has qualities akin to bot...

Fomes fomentarius, ayrıştırıcı ve parazit görevi gören, dünyanın kuzey yarımküresinde yayılımı olan bir makrofungustur [1,2,3]. Geleneksel Doğu Asya tıbbında kullanılmıştır [1,4]. Farklı preparasyonlarının ise inflamasyonda, çeşitli kanser türlerinde, hepatositozda, oral ülserde ve gastroenterik bozukluklarında kullanılmasının yanı sıra, antimikrob...

Mycorrhizal fungi host diverse mycoviruses that contribute to our understanding of their diversity and evolution. Here we report on the identification and complete genome characterization of three novel partitiviruses naturally infecting the ectomycorrhizal fungus Hebeloma mesophaeum. During NGS derived viral sequence analyses, we identified a part...

Background Multiple antibiotic resistance continues to be a problem of our time. For this reason, studies of multi-drug resistance continue increasingly, either by the discovery of new antibiotics or understanding the resistance mechanisms and propose solutions to prevent resistance. Bacteria have strong resistance mechanisms and efflux pumps are j...

Viruses that naturally infect fungal species and capable of establishing mycorrhizae are largely unknown. In this study, we identified and characterized a new partitivirus inhabiting the ascomycete, mycorrhizal desert truffle species Terfezia claveryi, and named it “Terfezia claveryi partitivirus 1” (TcPV1). The entire genome of TcPV1, sequenced by...

Macrofungi have long been used as food and medicinal purposes by humankind. This study aimed to identify a macrofungus sample collected from Niğde, Türkiye in 2019 via molecular techniques and determine the content of total phenolic compounds, antioxidant and antimicrobial activities. According to data obtained from the sequence analysis of ITS fra...

Mushrooms are among the natural products that are widely used in the treatment of diseases. In our study, the biological activities of Candolleomyces candolleanus (Fr.) D. Wächt. & A. Melzer were determined. Within the scope of the study, ethanol and methanol extracts of the basidiomata of the mushroom were obtained. Total antioxidant, total oxidan...

Studies to prevent antibiotic resistance are continuing rapidly. As it is known, bacteria have various resistance mechanisms. The over-expression of efflux pumps is just one of these resistance mechanisms. In antibiotic intake, the drug taken through this resistance mechanism is expelled out of the cell and the effect of the drug is lost. Various...

Studies on eliminating multi-antibiotic resistance are continuing and in these studies, the search for inhibitors takes a major role. Due to the toxic nature of synthetic efflux pump inhibitors, new inhibitors having low toxicity are under investigation. Although studies at the in vivo level are long-term, studies at the in vitro level are provided...

Antibiotics are compounds that can stop the reproduction or kill microorganisms, but for some reason, microorganisms in time show resistance to antibiotics. Using efflux pumps, the antibiotic entered into the bacterial cell is pumped out; therefore, it cannot act against bacteria. This situation increases antibiotic resistance in bacteria. Thus, mi...

The current study presents a Turkish Truffle Checklist based on literature and a newly discovered species. Within the two divisions, the list includes 104 species belonging to 35 genera and 20 families, as well as their range and Turkish names. Tuber oligospermum was one of them, and it was recorded for the first time in Turkey. A brief description...

Mushrooms are the only living groups that spread in different ecosystems. They stand out with their nutritional and medicinal properties. In this study, some biological activities of wild mushroom Laccaria laccata (Scop.) Cooke were determined. In this context, the ethanol extract of the mushroom was extracted in a soxhlet device. The antioxidant p...

In this study, antioxidant, oxidant, DPPH scavenger , antimicrobial and antiproliferative activities of wild edible mushroom Marasmius oreades (Bol-ton) Fr. were determined. In this context, ethanol, methanol and dichloromethane extracts of the fruit-ing bodies of the mushroom were obtained in a soxhlet device. Antioxidant and oxidant status were m...

The purpose of this study was to determine Fe, Cd, Cr, Se, P, Cu, Mn, Zn, Al, Ca, Mg, and K contents of some edible (Chlorophyllum rhacodes, Clavariadelphus truncatus, Clitocybe nebularis, Hydnum repandum, Hygrophorus pudorinus, Infundibulicybe gibba, Lactarius deliciosus, L. piperatus, L. salmonicolor, Macrolepiota mastoidea, Russula grata, Suillu...

Son yıllarda yapılan birçok çalışmada mantarlarda bulunan faydalı doğal bileşenlerin çeşitli hastalıkları iyileştirmede ve hastalık etkenini ortadan kaldırmada çok önemli olduğu bulunmuştur. Farklı ülkelerde yayılış gösteren mantarların biyolojik aktivitelerinin olduğu bildirilmiştir. Bu çalışmada Türkiye’de Entoloma sinuatum (Bull.) P. Kumm’un ant...

The complete genome of a novel mycovirus, Albatrellopsis flettii mitovirus 1 (AfMV1), hosted by the basidiomycetous ectomycorrhizal fungus Albatrellopsis flettii (Morse ex Pouzar) Audet, was sequenced and analyzed. The full-length cDNA sequence, obtained from a dsRNA replication intermediate of the AfMV1 genome, is 3037 bp in length with a predicte...

Pisolithus arhizus (Scop) Rauschert is a cosmopolitan mushroom in warm temperate regions of the world and forms ectomycorrhizas associations with a wide range of tree species (both angiosperms and gymnosperms). P. arhizus samples were collected from a pine forest in Antalya province (Turkey). Powdered mushroom samples were extracted with ethanol (E...

ORDU YÖRESİNDE BELİRLENEN YENEN MAKROMANTARLAR ve BAZILARININ TIBBİ ÖZELLİKLERİ

Mushrooms have been used as food and medicinal purposes for centuries. In this study, some edible Ascomycota were collected in 2018 and 2019 from Niğde, Turkey. Molecular identifications of the samples were performed afterward. PCR products were sent for sequence analysis after ITS fragments were amplified with suitable primers concerning DNA isola...

The use of synthetic-based packaging materials can causes health and environmental problems. To overcome these problems, researchers have focused on natural polymers such as cellulose and chitosan, which are easily degradable in nature. Herein, biodegradable composite films were prepared for the first time by including Pisolithus arhizus spores (PS...

The aim of this study was to determine the element content of wild edible and inedible mushroom species (Agaricus campestris, Armillaria ostoyae, Boletus reticulatus, Bondarzewia mesenterica, Bovistella utriformis, Cantharellus cibarius, Marasmius oreades, Megacollybia platyphylla, Meripilus giganteus, Neoboletus erythropus, Panellus stipticus, Pha...

Virus communities of uncultivated fungi stay largely unknown. In the current study, we characterized a new partitivirus species detected in the basidiomycetous, saprobic mushroom Leucocybe candicans, named "Leucocybe candicans partitivirus 1" (LcPV1). The full-length genome of LcPV1, determined using deep sequencing and RLM-RACE approaches, consist...

Mycoviruses widely exist in diverse lineages of fungi, yet there are only a few studies on mycovirus infection in uncultivated fungi. We here report the presence of a dsRNA mycovirus in saprotrophic spring orange peel fungus Caloscypha fulgens. A novel dsRNA virus, named “Caloscypha fulgens fusagravirus 1” (CfFV1), was isolated from a single ascoca...

The present study represents the antioxidant, anticancer, antimicrobial, and antibiofilm activities of Hydnum repandum, an edible and medicinal mushroom known as Wood Hedgehog. The ethanolic extract had a high amount of myricetin and apigenin and displayed antiproliferative effect against MCF-7 and HT-29 cell lines. Moreover, the extract displayed...

In this study, antioxidant, oxidant, and antimi-crobial activities of some Pholiota species (Pholiota adiposa (Batsch) P. Kumm., P. lubrica (Pers.) Singer and P. squarrosa (Vahl) P. Kumm.) were determined. Also, the Fe, Cu, Zn, Pb, Cr, Mn, Ni, and Cd levels accumulated in the fruiting bodies of these species were measured. Ethanol extracts of the m...

Two putative mycoviruses belonging to the proposed family “Fusariviridae” were identified in Morchella esculenta by sequencing of double-stranded RNAs extracted from the morel mushroom. These viruses were tentatively named “Morchella esculenta fusarivirus 1” (MeFV1) and “Morchella esculenta fusarivirus 2” (MeFV2). Including the poly(A) tail the com...

In the current study, antioxidant, oxidant, and antimicrobial potentials of Xylaria polymorpha (Pers.) Grev. and X. hypoxylon (L.) Grev. were determined. Also, the levels of elements accumulated by these fungi were measured. The samples were extracted with ethanol in soxhlet apparatus. Antioxi-dant and oxidant potentials were determined using Rel A...

Fungal samples were collected from Kazdağı National Park (Balıkesir/Turkey) on October 25, 2014 and they are identified by implementing both traditional methods and ITS rDNA-based molecular phylogenetic analysis. By taking into account the high sequence similarity between the collected samples (ANK DNZ 023) and Albatrellopsis flettii (Morse ex Pouz...

The ability of mushrooms to accumulate heavy metals has increased concerns over their toxic effects on human health in recent years. The aim of this study was to determine the metal contents (Zn, Fe, Co, Mn, Cu, Pb, Ni, and Cd), daily intake of metal (DIM) and health risk index (HRI) values of nineteen different mushroom species (edible, inedible,...

Natural resources have been the savior of people at every stage of human history. Mushrooms are very valuable food sources, especially in the rainy seasons. They can be grouped as poisonous, edible or inedible, depending on their nutritional status. These groups have positive or negative effects. Some types of mushrooms have different medicinal pro...

The current study was conducted based on a Battarrea sample obtained from Muğla province (Turkey). The sample was identified based on both conventional methods and ITS rDNA region-based molecular phylogeny. By taking into account the high sequence similarity between the sample (ANK Akata & Altuntaş 690) and Battarrea phalloides the relevant specime...

Viruses hosted by uncultivated fungi have been poorly studied. We carried out studies to characterize a large dsRNA segment (~20 kbp) detected in the basidiomycetous, ectomycorrhizal fungus Hygrophorus penarioides. The dsRNA was gel-purified and its randomly amplified cDNA fragments were used for high throughput sequencing (HTS). Reads were de novo...

Makromantarlar Ascomycota ve Basidiomycota bölümlerinde yer alan ve çıplak gözle ayırt edilebilen makroskobik mantarları içermektedirler. Makromantarlar yenir, yenmez ve zehirli olmak üzere kabaca üç grup altında toplanır. Yenmez özellikteki mantarlar zehirli etki göstermez ancak bu türler sert yapısı, kokusu ve tadı nedeniyle tercih edilmezler. Ze...

Yeryüzünde yaşayan en eski kara bitkileri olan briyofitler evrimsel açıdan algler, mantarlar ve likenlere göre daha gelişmiş, eğreltiler ve tohumlu bitkilere göre ise daha ilkel yapıya sahiptirler. Yaşamlarını devam ettirebilecekleri düzeyde suyun ve nemin olduğu tropikal bölgelerden ılıman bölgelere, çöllerden kutuplara kadar hemen hemen dünya üze...

Mantar ile alg veya siyobakteri birlikteliğiyle tek bir tallus oluşturmak üzere biraraya gelen simbiyotik ortaklığa liken adı verilir. Likenin mantar ortağına mikobiyont, fotosentetik ortağına fotobiyont adı verilir. Mikobiyont, liken tallusunun ihtiyacı olan su, karbondioksit, mineral madde ve barınak sağlanması ile görevlidir. Fotobiyont ise bu y...

Natural mushrooms are one of the important elements of the ecosystem. It has been preferred by people from past to present because of its nutritional and medicinal potentials. In this study, antioxidant, oxidant and antimicrobial potential of Trametes hir-suta (Wulfen) Lloyd has been determined. In addition , phenolic compounds in its fruiting body...

The aim of this study was to determine the essential element (Zn, Ca, K, Fe, Na, and Mg), essential trace element (Co, Mn, Cr, and Cu), and non-essential element (Pb, Ni, and Cd) contents of eight different Russula species (R. risigallina (Batsch) Sacc., R. cyanoxantha (Schaeff.) Fr., R. delica Fr., R. vinosa Lindblad, R. olivacea (Schaeff.) Fr., R...

Virus populations of uncultivated fungi remain scarcely studied. In the present study, we characterized a new partitivirus isolated from the false morel mushroom Gyromitra esculenta, named “Gyromitra esculenta partitivirus 1” (GePV1). The complete genome of GePV1, whose sequence was determined by combining high-throughput sequencing and RLM-RACE ap...

Wild edible mushrooms are very popular for both their flavors and nutritional values. However, some mushroom species can be harmful to human health as they accumulate some elements in excessive amounts. The aim of this study was to determine the concentrations of Al, from Belgrad forest (Istanbul, Turkey). Daily intakes of element (DIE) and health...

In this study, antioxidant, oxidant, antimicrobial activity and heavy metal contents of Morchella esculenta (L.) Pers. mushroom collected from Konya (Turkey) province were determined. Antioxidant and oxidant values were determined by using Rel Assay TAS and TOS kits. Element contents were determined by AAS (atomic absorption spectroscopy). Antimicr...

The purpose of the present work is to identify Tulostoma samples collected from Ankara and Kırıkkale provinces (Turkey). Both traditional methods and ITS rDNA-based molecular phylogeny were implemented to identify the specimens. When the high sequence similarities were taken into account, the collected specimens ANK Akata & Altuntaş 647 and ANK Aka...

The current study was carried out based on fungi specimens collected from Rhus coriaria in Bolu province in July 2018. As a result of field and laboratory studies, Synnemasporella aculeans (Schwein.) X.L. Fan & J.D.P. Bezerra (Synnemasporellaceae) was reported for the first time from Turkey at species, genus and family level and Rhus coriaria was r...

Virus populations of ectomycorrhizal fungi remain poorly studied. In the present study, we characterized a new partitivirus isolated from the basidiomycetous, ectomycorrhizal fungus Hygrophorus penarioides, named “Hygrophorus penarioides partitivirus 1” (HpPV1). The whole genome of HpPV1, determined by merging deep sequencing and RLM-RACE approache...

The present study is based on moss specimens collected from several urban habitats in Ankara University Beşevler 10. Yıl Campus in the year 2019. As a result of identifications of 94 bryophyte specimens collected from the campus area, a total of 28 species belonging to 7 families and 17 genera were determined. Six of them are new record for B7 grid...

Aim of the study: The current study aims to contribute Turkish powdery mildews by adding newly recorded fungus. Area of study: The northern slopes of Derebağ waterfall was the study area located within the borders of Derebağ Town (Yahyalı/Kayseri/Turkey) district in the northeast of Aladağlar. Material and methods: Infected maple specimens were col...

Mushrooms are very important natural source of foods and medicines. More than 600 mushroom species are used for food or medicine due to their pharmacologically active substances and essential nutrients. Medicinal mushroms are those which produce medically active compounds and they have been used as medicine since ancient time. Because of its suitab...

Tuber samples were collected from Kırklareli province on the 10 th of August 2020 and they are identified by implementing both traditional methods and molecular phylogenetic analysis using the rDNA sequences including Internal Transcribed Spacer (ITS) and 28S Ribosomal Large Subunit (LSU) regions. By taking into account the high sequence similarity...

In this study, sixty-one species of Powdery mildew (Erysiphales) in Aladağlar and Bolkar mountains were determined. The research was carried out between 2013 and 2016. The sixty-one species of Powdery mildew were observed from 37 family, 83 genera and 94 host species and make a contribution to Turkish mycobiota.

Mushrooms have been used extensively, owing to their nutritional and medicinal value, for thousands of years. This study designed for the determine of antioxidant and antimicrobial potential of two edible mushrooms Armillaria mellea (Vahl) P.Kumm. and Macrolepiota procera (Scop.) Singer. Antioxidant activity was detected method by DPHH free radical...

Viruses hosted by ectomycorrhizal fungi remain poorly studied. In this study, we detected eight new fungal viruses co-infecting a single isolate of the hypogeous ectomycorrhizal fungus Picoa juniperi using high-throughput sequencing. Phylogenetic analysis of one identified virus abbreviated as PjMTV1 revealed its closest relatives as members of the...

Türkiye’de “kuzugöbeği” olarak bilinen Morchella cinsi, Türkiye’nin de içinde bulunduğu birçok ülkede yoğun olarak tüketilmekte ve bilimsel olarak da üzerindeki ilgiyi her zaman korumaktadır. Bu mantar cinsinde yapılan sistematik çalışmalar popülerliğini sürdürmekte, yeni türler tanımlanmakta ve sinonimler belirlenmektedir. Sunulan bu çalışmada da...

The aim of this study is to analyze Fe, Mn, Cu, Zn, Co, Cd, Pb, and Ni contents of Cyclocybe cylindracea, Armillaria mellea, Bjerkandera adusta, Rheubarbariboletus armeniacus, Coprinellus disseminatus, C. micaceus, C. comatus, Inonotus hispidus, Lepista nuda, Leucoagaricus leucothites, Pleurotus ostreatus, Cerioporus squamosus, Schizophyllum commun...

Amatoxins, most of which are hepatotoxic, can cause fatal intoxication. While mushrooms in the amatoxin-containing Galerina genus are rare, they can poison humans and animals worldwide. Few studies have profiled the toxicity of Galerina marginata. In addition, many studies indicate that macrofungi can have different characteristics in different reg...

Modern people have increased their living standards due to the development of technology. However, health problems have increased with increasing living standards. Efforts to return to nature and natural products such as herbal drugs, medicinal mushrooms and traditional medicine practices have become popular. Medical mushrooms, which have an import...