Ildiko Pecsi

Ildiko Pecsi
University of Pécs | PTE · Department of Biophysics

PhD

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10
Publications
1,259
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233
Citations

Publications

Publications (10)
Article
Full-text available
Unlabelled: Succinate:quinone oxidoreductase (Sdh) is a membrane-bound complex that couples the oxidation of succinate to fumarate in the cytoplasm to the reduction of quinone to quinol in the membrane. Mycobacterial species harbor genes for two putative sdh operons, but the individual roles of these two operons are unknown. In this communication,...
Article
Full-text available
Thymidine biosynthesis is essential in all cells. Inhibitors of the enzymes involved in this pathway (e.g. methotrexate) are thus frequently used as cytostatics. Due to its pivotal role in mycobacterial thymidylate synthesis dUTPase, which hydrolyzes dUTP into the dTTP precursor dUMP, has been suggested as a target for new antitubercular agents. Al...
Data
Primers used in the present study. (DOCX)
Data
Key plasmids used in the generation of dut deletion mutant M. smegmatis. (A) p2Nbk-duth delivery vector used to generate mutant SCOs. The 2.1 kb HindIII M. smegmatis fragment indicated in Figure 3 was inserted into the p2NIL vector to construct the delivery vector. The dut allele was disrupted with a 1.8 kb fragment encoding hygromycin resistance,...
Article
Full-text available
We investigated the potential (d)NDP/(d)NTP discrimination mechanisms in nucleotide pyrophosphatases. Here, we report that dUTPase, an essential nucleotide pyrophosphatase, uses a C-terminal P-loop-like sequence in a unique mechanism for substrate discrimination and efficient hydrolysis. Our spectroscopy and transient kinetics results on human dUTP...
Article
Full-text available
Autophagy is a lysosome-mediated self-degradation process of eukaryotic cells that, depending on the cellular milieu, can either promote survival or act as an alternative mechanism of programmed cell death (PCD) in terminally differentiated cells. Despite the important developmental and medical implications of autophagy and the main form of PCD, ap...
Article
Full-text available
Aromatic interactions are well-known players in molecular recognition but their catalytic role in biological systems is less documented. Here, we report that a conserved aromatic stacking interaction between dUTPase and its nucleotide substrate largely contributes to the stabilization of the associative type transition state of the nucleotide hydro...
Article
The phosphate-binding loop (P-loop) or Walker A sequence is a common feature of a large number of ATP and GTP binding proteins including kinases, cytoskeleton and DNA motors, membrane pumps and transporters. All known P-loop containing proteins are able to sense the difference between bound NTP and NDP via their P-loop which allows for hydrolyzing...

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