Ildikó Pál

Ildikó Pál
Gedeon Richter Plc

PhD

About

16
Publications
2,085
Reads
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158
Citations
Additional affiliations
October 2016 - present
Gedeon Richter Plc
Position
  • Researcher
January 2012 - September 2016
Hungarian Academy of Sciences
Position
  • Research Assistant
July 2010 - December 2013
Hungarian Academy of Sciences
Education
September 2011 - November 2015
Eötvös Loránd University Chemistry Doctoral School
Field of study
  • Synthetic Chemistry, Materials Science and Biomolecular Chemistry Doctoral program
September 2005 - June 2010
Eötvös Loránd University
Field of study
  • Neuro- and Human Biology

Publications

Publications (16)
Article
Full-text available
The use of SU-8 material in the production of neural sensors has grown recently. Despite its widespread application, a detailed systematic quantitative analysis concerning its biocompatibility in the central nervous system is lacking. In this immunohistochemical study, we quantified the neuronal preservation and the severity of astrogliosis around...
Article
Aims In this study, we introduce an edge-type laminar silicon probe suitable for improved cell accessibility during in vitro brain slice recordings. With protruding contact sites, the spiky probe provides high signal yield and quality while approaching cells located deeper in the tissue. Methods. The spiky probe comprises an angled shank carrying 3...
Article
Full-text available
Neural interface technologies including recording and stimulation electrodes are currently in the early phase of clinical trials aiming to help patients with spinal cord injuries, degenerative disorders, strokes interrupting descending motor pathways, or limb amputations. Their lifetime is of key importance; however, it is limited by the foreign bo...
Article
Full-text available
Background Voltage-sensitive dye (VSD) imaging and intrinsic optical signals (IOS) are widely used methods for monitoring spatiotemporal neural activity in extensive networks. In spite of that, identification of their major cellular and molecular components has not been concluded so far. Results We addressed these issues by imaging spatiotemporal...
Article
Full-text available
Hippocampal sharp wave-ripples (SPW-Rs) occur during slow wave sleep and behavioral immobility and are thought to play an important role in memory formation. We investigated the cellular and network properties of SPW-Rs with simultaneous laminar multielectrode and intracellular recordings in a rat hippocampal slice model, using physiological bathin...
Article
Polyamidoamine (PAMAM) dendrimers are highly charged hyperbranched protein-like polymers that are known to interact with cell membranes. In order to disclose the mechanisms of dendrimer-membrane interaction, we monitored the effect of PAMAM generation five (G5) dendrimer on the membrane permeability of living neuronal cells followed by exploring th...
Article
Full-text available
Background The potential nanocarrier polyamidoamine (PAMAM) generation 5 (G5-NH2) dendrimer has been shown to evoke lasting neuronal depolarization and cell death in a concentration-dependent manner. In this study we explored the early progression of G5-NH2 action in brain tissue on neuronal and astroglial cells. Results In order to describe early...
Article
Full-text available
Widely used for mapping afferent activated brain areas , the label-free intrinsic optical signal (IOS) is mainly ascribed to blood volume changes subsequent to glial glutamate uptake. By contrast, IOS imaged is generally attributed to neuronal and glial cell swelling, however the relative contribution of different cell types and molecular players r...
Data
Overview of the temporal changes of afferent evoked IOS amplitude. The video shows the IOS amplitude map starting at the onset of the first stimulus (0 ms, 1st frame) to the end of the experiment (9800 ms, 164th frame). The stimulation lasts for 450 ms (frames #2–7). Each pixel corresponds to one photodiode of the PDA detector and covers an approxi...
Data
Comparison of the effect of different drug on IOS in the sole CA1 and CA3 as well as in the whole slice. Changes of sum of all IOS parameters calculated for the sole CA1 (black), CA3 (red) and the whole slice (blue) are compared. Except in the case of DHK, the effects of the drugs were not significantly different for the sole CA1 and CA3 when compa...
Data
Temporal pattern of IOS generation. The video shows the IOS amplitude map starting at the onset of the first stimulus (0.6 ms, 2nd frame) to the onset of the third stimulus (100 ms, 165th frame). The second stimulus is applied at 50 ms (83rd frame). Each pixel corresponds to one photodiode of the PDA detector and covers an approximately 70×70 µm ar...
Article
Full-text available
Accumulating evidence suggests that different energy metabolites play a role not only in neuronal but also in glial signaling. Recently, astroglial Ca(2+) transients evoked by the major citric acid cycle metabolite succinate (SUC) and gamma-hydroxybutyrate (GHB) that enters the citric acid cycle via SUC have been described in the brain reward area,...
Data
File name: 3-31-29_PM-diff.avi. Ca2+ signaling evoked by GHB and ATP in the acute NAc slice from the rat brain. Movie of time measurement showing GHB (50 μM) and ATP (100 μM) application onto a Fluo-4 AM loaded acute, 300 μm thick NAc slice. GHB was applied between 0 and 2 min in the perfusion buffer. ATP was applied for 60 s starting from 1.5 min...
Article
Full-text available
We report for the first time on neuronal signaling for the evaluation of interactions between native plasmamembrane and polyamidoamine (PAMAM) dendrimers. Generation 5 polycationic (G5-NH(2)), novel β-D-glucopyranose-conjugated G5-NH(2) and generation 4.5 polyanionic (G4.5-COONa) polyamidoamine (PAMAM) dendrimers (1-0.0001 mg/ml) were applied in ac...
Article
INTRODUCTION: The molecular interactions and cell-biological mechanisms behind the adhesive behaviour of cells are not properly understood. By recording material deposition in a narrow layer (100-200 nm above the sensor surface), the OWLS assay provide real-time data on surface – cell membrane contacts, on membrane-derived shred material and compou...

Questions

Question (1)
Question
I am new to the field of calcium imaging. I used OGB-1 AM dye at 2 uM concentration for 1 hour at 35 C in adult rat hippocampal slices. Only neurons in the stratum radiatum were loaded and the pyramidal cells were not. Bootman et al 2013 claim in their article that if they stain cell cultures above room temperature they inhibit the multidrug resistance-associated proteins (MRP) for better dye loading. Can MRP activity cause also uneven dye loading in the slices? I would be grateful for any suggestions.

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Projects

Project (1)
Project
Specify and set-up high temporal and spatial resolution two-photon microscopy in a pharma industry environment to be used for neurological disorders drug research. Establish proper experimantal methods to support ongoing research projects.