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Molecular Epidemiology
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Publications
Publications (34)
Figure 1. Self-collected mouthwash–saliva (MWS) and RT-qPCR-based protocol for SARS-CoV-2 detection incorporated to a surveillance web–mobile application platform system to integrate real-time epidemiological and clinical core baseline database with laboratory results for decision making on testing, confining, and self-report to family–social–cowor...
Figure 1. Self-collected mouthwash–saliva (MWS) and RT-qPCR-based protocol for SARS-CoV-2
detection incorporated to a surveillance web–mobile application platform system to integrate realtime
epidemiological and clinical core baseline database with laboratory results for decision making
on testing, confining, and self-report to family–social–cowork...
This study provides a safe and low-cost in-house protocol for RT-qPCR-based detection of SARS-CoV-2 using mouthwash–saliva self-collected specimens to achieve clinical and epidemiological surveillance in a real-time web environment applied to ambulatory populations. The in-house protocol comprises a mouthwash–saliva self-collected specimen, heat vi...
Citation: Mora-Aguilera, G.; Martínez-Bustamante, V.; Acevedo-Sánchez, G.; Coria-Contreras, J.J.; Guzmán-Hernández, E.; Flores-Colorado, O.E.; Mendoza-Ramos, C.; Hernández-Nava, G.; Álvarez-Maya, I.; Gutiérrez-Espinosa, M.A.; et al. Surveillance Web System and Mouthwash-Saliva qPCR for Labor Ambulatory SARS-CoV-2 Detection and Prevention. Int. J. E...
p>The emergence of COVID-19, a disease caused by SARS-CoV-2, was first reported in December 2019. It then spread in pandemic proportions, creating a human health emergency that was declared a pandemic by the World Health Organization. The airborne transmission of the SARS-CoV-2 virus, mainly through droplets of saliva, has been highly effective, de...
Whole genome sequencing (WGS) analysis in tuberculosis allows the prediction of drug-resistant phenotypes, identification of lineages, and to better understanding of the epidemiology and transmission chains. Nevertheless the procedure has been scarcely assessed in Mexico, in this work we analyze by WGS isolates of Mycobacterium tuberculosis circula...
Introduction:
The US-Mexico region is at high risk of elevated tuberculosis (TB) incidence due to mobility and migration. Knowledge of how socio-demographic factors varies geographically, provides clues to understanding the determinants of tuberculosis and may provide guidance for regional prevention and control strategies to improve public health...
Objectives:
To analyze the frequency of gene mutations associated to drug resistant in clinical samples from population of the Jalisco state (Mexico) and then evaluate the genetic variability of TB and MDR-TB strains to describe the frequency of M. tuberculosis families.
Methods:
We analyzed the clinical isolates of M. tuberculosis obtained from...
Introduction. The bacteria that causes tuberculosis, Mycobacterium tuberculosis,
is known for the high rate of drug resistance, causes health problems and affects
the economy to public health worldwide. In Mexico, the presence and persistence
of tuberculosis remains a major problem. The annual number of identified cases
increased by 15% every year....
Non-invasive biological indicators of the absence/presence or progress of
the disease that could be used to support diagnosis and to evaluate the effectiveness of
treatment are of utmost importance in Duchenne Muscular Dystrophy (DMD). This
neuromuscular disorder affects male children, causing weakness and disability, whereas
female relatives are a...
Non-invasive biological indicators of the absence/presence or progress of the disease that could be used to support diagnosis and to evaluate the effectiveness of treatment are of utmost importance in Duchenne Muscular Dystrophy (DMD). This neuromuscular disorder affects male children, causing weakness and disability, whereas female relatives are a...
Microarray technology is a powerful tool to investi-gate whole genome expression profiles to study the crosstalk between pathogens and associated hosts that cause illness. Microarrays have been used in several comparative genome hybridization studies of patho-gens. In addition to detection and identification of pathogens, microarrays are ideal for...
During ethanol fermentation, yeast cells are exposed to stress due to the accumulation of ethanol, cell growth is altered and the output of the target product is reduced. For Agave beverages, like tequila, no reports have been published on the global gene expression under ethanol stress. In this work, we used microarray analysis to identify Sacchar...
Hepatitis C virus (HCV) is a major cause of chronic hepatitis and hepatocellular carcinoma worldwide. The purpose of this study was to determine how the HCV structural proteins affect the dynamic structural and functional properties of hepatocytes and measure the extra-hepatic manifestations induced by these viral proteins. A transgenic mouse model...
Influenza A virus is a major cause of morbidity and mortality worldwide. There is a large knowledge base on the immune response to influenza. However, few studies have focused on global gene expression in immune cells after antigenic challenge. A better understanding of the host immune response is required for the development of more efficient mean...
For the purpose of gene identification, we propose an approach to gene expression data mining that uses a combination of unsupervised and supervised learning techniques to search for useful patterns in the data. The approach involves validation and elimination of irrelevant data, extensive data pre-processing, data visualization, exploratory cluste...
CD40, a glycoprotein expressed on B lymphocytes plays an important role in B cell development, growth and differentiation. The ligand for the CD40 is a 39-kDa glycoprotein (CD154) expressed onthe surface of activated T lymphocytes and is essential for thymus-dependent humoral immunity. The expression of CD154 is tightly regulated and its transient...
With the goal of performing astrocyte-specific modification of genes in the mouse, we have generated a transgenic line expressing Cre recombinase under the control of the human glial fibrillary acidic protein (hGFAP) promoter. Activity was monitored by crossing the hGFAP-cre transgenics with either of two reporter lines carrying a lacZ gene whose e...
With the goal of performing astrocyte-specific modification of genes in the mouse, we have generated a transgenic line expressing Cre recombinase under the control of the human glial fibrillary acidic protein (hGFAP) promoter. Activity was monitored by crossing the hGFAP-cre transgenics with either of two reporter lines carrying a lacZ gene whose e...
Recently, we synthesized a nonviral gene vector capable of transfecting cell lines taking advantage of neurotensin (NT) internalization. The vector is NT cross-linked with poly-L-lysine, to which a plasmid DNA was bound to form a complex (NT-polyplex). Nigral dopamine neurons are able to internalize NT, thus representing a target for gene transfer...
We report herein the synthesis of a novel DNA delivery system and in vitro evidence of its ability to transfect cell lines by binding to the high-affinity neurotensin receptor and subsequent internalization of ligand-receptor complexes. The targeting vehicle consisted of neurotensin crosslinked with poly-l-lysine via N-succinimidyl-3-(2-pyridyldith...
