Iker Garcia

Biocruces Health Research Institute · Neuromuscular Disorders Group

Excessive subconjunctival scarring is the main reason of failure of glaucoma filtration surgery. We analyzed conjunctival and systemic gene expression patterns after non penetrating deep sclerectomy (NPDS). To find expression patterns related to surgical failure and their correlation with the clinical outcomes. This study consisted of two consecuti...

The mean expression values of the genes analyzed in the blood samples. (XLSX)

The mean expression values of the genes analyzed in conjunctiva samples. (XLSX)

Selection of reference genes for normalisation of dystrophin mRNA RT-qPCR data Iker Garcia-Jimenez, Estíbaliz Ruiz-Del-Yerro, Virginia Arechavala-Gomeza Neuromuscular Disorders Group, BioCruces Health Research Institute, Barakaldo, Spain Accurate quantification of either total levels of dystrophin mRNA or dystrophin exon skipping is vital not onl...

Multicenter quantification of dystrophin exon 51 skipping

Background: Copy Number Variants (CNVs) contribute to a large fraction of genetic diversity and some of them have been reported to offer an evolutionary advantage. Aim: To identify CNVs in pigmentary loci that could contribute to human skin pigmentation diversity. Subjects and methods: This study assessed the existence of CNVs in every exon of...

Purpose: To evaluate the sensitivity and specificity of a PCR-strip system based on reverse dot blot for detection of MUC5AC mRNA in corneal epithelium samples from patients with limbal stem cell deficiency (LSCD), and to determine the correlation with clinical diagnosis. Methods: We obtained 87 corneal impression cytology (IC) samples from 55 s...

Dry eye and Meibomian gland dysfunction are common ocular surface disorders. Discrimination of both conditions often may be difficult given the overlapping of signs and symptoms, and the lack of correlation with clinical parameters. A total of 144 individuals were included in this study. To search for proteome differences, tear proteins were collec...

To evaluate a limbal stem cell deficiency (LSCD) diagnosis method based on the detection of the MUC5AC transcript by reverse transcription-polymerase chain reaction (RT-PCR) in comparison with the standard diagnostic method based on goblet cell detection by periodic acid-Schiff (PAS)-hematoxylin staining, using samples obtained from corneal epithel...

Small ruminant lentiviruses (SRLV) are widely spread in many countries, including Spain. However, little is known about the genetic characteristics of Spanish goat and sheep SRLV. In this study, segments from three genomic regions (pol, gag-p25 and LTR) were amplified using DNA isolated from three Spanish autochthonous sheep (one) and goats (two)....

Although the combination of pale skin and intense sun exposure results in an important health risk for the individual, it is less clear if at the population level this risk has possessed an evolutionary meaning. In this sense, a number of adaptive hypotheses have been put forward to explain the evolution of human skin pigmentation, such as photopro...

Gene gun mucosal DNA immunization of sheep with a plasmid expressing the env gene of Maedi-Visna virus (MVV) was used to examine the protection against MVV infection in sheep from a naturally infected flock. For immunization, sheep were primed with a pcDNA plasmid (pcDNA-env) encoding the Env glycoproteins of MVV and boosted with combined pcDNA-env...

El tropismo es uno de los mecanismos fundamentales para comprender la patogenicidad de los lentivirus. Dado que el Maedi Visna ovino (VMV) infecta a la línea monocito-macrófago, su detección a partir de células sanguíneas ovinas por PCR puede presentar dificultades, pues el porcentaje de monocitos en sangre ovina así como el número de copias de DNA...