Iines Salonen

Japan Agency for Marine-Earth Science Technology | JAMSTEC

Metabarcoding is a method that combines high-throughput DNA sequencing and DNA based identification. Previously, this method has been successfully used to target spatial variation of eukaryote communities in marine sediments, however, the temporal changes in these communities remain understudied. Here, we follow the temporal changes of the eukaryot...

Foraminifera are ubiquitous marine protists with an important role in the benthic carbon cycle. However, morphological observations often fail to resolve their exact taxonomic placement and there is a lack of field studies on their particular trophic preferences. Here, we propose the application of metabarcoding as a tool for the elucidation of the...

Benthic foraminifera are known to play an important role in marine carbon and nitrogen cycles. Here, we report an enrichment of sulphur cycle-associated bacteria inside intertidal benthic foraminifera (Ammonia sp. (T6), Haynesina sp. (S16) and Elphidium sp. (S5)), using a metabarcoding approach targeting the 16S rRNA and aprA-genes. the most abunda...

Metabarcoding analyses of bacterial and eukaryotic communities have been proposed as efficient tools for environmental impact assessment. It has been unclear, however, to which extent these analyses can provide similar or differing information on the ecological status of the environment. Here, we used 16S and 18S rRNA gene metabarcoding to compare...

Foraminifera are unicellular eukaryotes that are an integral part of benthic fauna in many marine ecosystems, including the deep sea, with direct impacts on benthic biogeochemical cycles. In these systems, different foraminiferal species are known to have a distinct vertical distribution, i.e., microhabitat preference, which is tightly linked to th...

Supplementary Material of the article "Metabarcoding Insights Into the Trophic Behavior and Identity of Intertidal Benthic Foraminifera" https://doi.org/10.3389/fmicb.2019.01169

The ultrastructure of the living foraminiferan, Ammonia sp. (phylotype unknown), collected from surficial and deeper, subsurface (anoxic) sediments from the Dutch Wadden Sea, was examined to provide information on the physiology of the foraminiferal cell and its adaptive strategies to low oxygen conditions. The observed changes in cell ultrastructu...

Despite the worldwide occurrence of marine hy-poxic regions, benthic nitrogen (N) cycling within these areas is poorly understood and it is generally assumed that these areas represent zones of intense fixed N loss from the ma-rine system. Sulfate reduction can be an important process for organic matter degradation in sediments beneath hypoxic wate...

Sulfate-reducing bacteria (SRB) can greatly impact benthic nitrogen (N) cycling, by for instance inhibiting coupled denitrification-nitrification through the production of sulfide or by increasing the availability of fixed N in the sediment via dinitrogen (N2)-fixation. Here, we explored several coastal and deep-sea benthic habitats within the Atla...