Igor Vladimirovich Yaminsky

Lomonosov Moscow State University | MSU · Faculty of Physics

Dynamic light scattering, atomic force and transmission electron microscopy were used to characterize three different types of potential carriers of double-stranded RNA (dsRNA): calcium phosphate particles, chitosan particles, and hybrid particles consisting of an inorganic core coated with chitosan. The hydrodynamic diameter and electrokinetic pot...

Modern scanning probe microscopes using piezoceramic stage for scanning are becoming increasingly important metrological equipment. However, this piezoceramic stage has their shortcomings such as creep, non-linearity and hysteresis. To eliminate movement errors it is necessary to additionally calibrate the microscope using various measures. Goals:...

The article deals with current issues of neurobiology in the direction of scanning capillary microscopy of neurons. The study of living neurons by the means of probe microscopy makes it possible not only to obtain 3D images, but also to carry out continuous measurements, evaluate axon and dendrite growth parameters, and determine the mechanical pro...

The article presents data on the sporulation process of Brevibacillus laterosporus, which are of interest due to their biological activity. Crystalline and acrystalline strains of Brevibacillus laterosporus have insecticidal properties.

The article discusses examples of scanning capillary microscopes implementation. Special attention is paid to the fundamental diagrams of their design and the issue of integration into a capillary microscope of a system for supporting the vital activity of living biological samples. When studying living systems using the capillary microscopy, it is...

The article is devoted to the study of living systems using one of the advanced imaging methods — scanning capillary or ion-conductance microscopy. It belongs to the probe family, but has significant differences from related techniques, opening up new opportunities for visualizing the dynamics of living objects in the natural environment over a lon...

In this paper, we propose a technology for the rapid and sensitive detection of the whole viral particles of SARS-CoV-2 using double-labeled DNA aptamers as recognition elements together with the SERS method for detecting the optical response. We report on the development of a SERS-aptasensor based on a reproducible lithographic SERS substrate, fea...

Biosensors combining the ultrahigh sensitivity of surface-enhanced Raman scattering (SERS) and the specificity of nucleic acid aptamers have recently drawn attention in the detection of respiratory viruses. The most sensitive SERS-based aptasensors allow determining as low as 10⁴ virus particles per mL that is 100-fold lower than any antibody-based...

The addition of a dielectric transparent microlens in the optical scheme is an effective and at the same time simple and inexpensive way to increase the resolution of a light microscope. For these purposes, spherical, and cylindrical microlenses with a diameter of 1‐100 μm are usually used. The microlens focuses the light into a narrow beam called...

Extreme field localization and giant field enhancement are often achieved by using plasmonic nanostructures and metamaterials such as strongly coupled silver nanoparticles.Dielectric particles and structures can focus light beyond the diffraction limit (photonic nanojet effect), but with much weaker strengths. Recently, we showed that dielectric mi...

The article tells about carcinoma cells morphology measured by capillary microscopy which allows not only the visualization in a three-dimensional scale, but also provides an opportunity to process a number of experimental data for diagnostic purposes.

The article tells about atomic force microscopy of blood cells, which allows not only visualization in a three- dimensional scale, but also provides an opportunity to process a number of data for diagnostic purposes.

The article is devoted to the study of erythrocytes using a device for combined microlens and probe microscopy. With its help, it is possible to register the kinetics of the transformation of an erythrocyte into an echinocyte in an optical microscope without the use of additional labels or fixation.

The article discusses high-speed probe microscopy of biological objects and software for processing the results. The study of biological objects using probe microscopy does not require complex sample preparation, but direct selection of the optimal scanning mode requires experience and practical skills.

Scanning probe microscopy is a group of measurements that provides 3D visualization of viruses in different environmental conditions including liquids and air. Besides 3D topography it is possible to measure the properties like mechanical rigidity and stability, adhesion, tendency to crystallization, surface charge, etc. Choosing the right substrat...

Scanning probe microscopy and its effective applications for medical purposes are discussed. An overview of the possibilities and prospects of using the results of probe microscopy in biomedicine is given. The principles of measurement and the advantages of scanning probe microscopy over other diagnostic methods, such as high-resolution optical mic...

The article is devoted to the study of viruses and bacteria using a scanning probe microscope in atomic force mode, in particular, to the features of sample preparation, interpretation of the data obtained, and image processing.

The article is devoted to optical microlens microscopy, which, in combination with scanning probe microscopy, can significantly improve the process of measuring biological objects in a natural environment.

The article is devoted to the study of viruses and bacteria using a scanning probe microscope in the atomic force microscopy mode, in particular, to the question, what data can be obtained using this method and how to interpret it.

The article tells about scanning probe microscopy, which has recently become a powerful tool for characterizing the structures and properties of living biological systems in aquatic conditions with unprecedented spatial resolution.

Tobacco mosaic virus-like nanoparticles and nanocellulose are materials with great potential for use in bionanotechnology. We used atomic force microscopy to study the interaction between these materials at the nanoscale. It was found that they could form a composite in which the nanocellulose fibers constituted a three-dimensional scaffold covered...

Electromechanical biosensors allow detecting viruses with high sensitivity – up to single viral particles. In this work, electromechanical systems based on cantilever biochips are used to detect biological agents.

Probe microscopy allows obtaining unique data on the viruses local physic-mechanical properties, while the preparation procedure, unlike other methods, is simple and does not require additional reagents. With the help of scanning probe microscopy, it is possible to obtain high resolution - up to 1 angstrom.

We describe rapid, label-free detection of Influenza A viruses using the first radial mode of oscillations of lead zirconate titanate (PZT) piezoelectric discs with a 2 mm radius and 100 µm thickness fabricated from a piezoelectric membrane. The discs are modified with a synthetic sialylglycopolymer receptor layer, and the coated discs are inserted...

Highly sensitive and rapid technology of surface enhanced Raman scattering (SERS) was applied to create aptasensors for influenza virus detection. SERS achieves 10⁶−10⁹ times signal amplification, yielding excellent sensitivity, whereas aptamers to hemagglutinin provide a specific recognition of the influenza virus. Aptamer RHA0385 was demonstrated...

Design of SERS substrate. Substrate length is 20 mm and width is 4 mm. (TIFF)

SERS spectra of fluorescent dyes Cy3 and BDP FL. (TIF)

We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions. Moreover, we showed that the TMV VLPs could associate strongly with nanocellulose slurry after a simple mixing step. We sequentially exposed nanocell...

Приведены результаты сотрудничества ученых МГУ имени М.В.Ломоносова и Технологического университета имени Шарифа (Тегеран, Иран). Проведен мастер-класс по зондовой микроскопии в Тегеране для студентов, аспирантов и научных сотрудников. Методом сканирующей зондовой микроскопии исследованы образцы оксида графена и тонких пленок оксида железа в рамках...

Graphite oxide has a complex structure that can be modified in many ways to obtain materials for a wide range of applications. It is known that the graphite precursor has an important role in the synthesis of graphite oxide. In the present study, the basal-plane surface of highly annealed pyrolythic graphite (HAPG) was oxidized by Hummers' method a...

Additional atomic force microscopy results.

The process of open-porous structure development in high-density polyethylene (HDPE) films during uniaxial deformation in supercritical carbon dioxide (SC-CO2) fluid at 35 °C and 10 MPa has been studied and visualized by means of atomic force microscopy. We suggest that the supercritical fluid act as adsorption-active medium, and the porous structu...

The results of the first year of cooperation of scientists of the Lomonosov Moscow State University and Sharif University of Technology (Tehran, Iran) are presented. The purpose of the project "Initiation of local chemical reactions in deposited thin films using scanning probe microscopy" is to determine the effect of the environment on the nature...

Graphite oxide is a compound, promising for different applications. Graphite basis plays an important role in synthesis process of graphite oxide. Two different graphite basis: highly oriented pyrolythic graphite (HOPG) and highly annealed pyrolythic graphite (HAPG), were used as a graphite precursor and oxidized by Hummers’ method. HAPG has surfac...

How to quickly learn 3D microscopy and 3D nanotechnology? It is necessary to become a participant of the Nanotechnology youth innovation creativity centre and a listener of the courses "3D Design in SolidWorks and Machining" and "Scanning Probe Microscopy". Modern machining centers manufacture parts with nanometer accuracy, and scanning probe micro...

Carbon nanomaterials, such as carbon nanotubes, graphene, and graphene oxide, are perspective for application in electronics, energy storage, aerospace and defense industries, biomedicine, etc. In the chapter, we briefly overview the structure, unique properties, current and future applications of carbon nanomaterials. Their effects on living syste...

Рассмотрены конструкция пьезокерамического кантилеверного биочипа и способы обнаружения с его помощью белковых макромолекул для использования в медицине. The design of the piezoceramic cantilever biochip and methods for detecting protein macromolecules for use in medicine are considered.

2D наноразмерных структур для энергонакопителей и катализаторов, а также проводить их контролируемое нанесение с помощью направленной литографии и локального анодного окисления. Установка позволяет получать экспериментальные данные о распределении электрического потенциала, тока заряда и разряда, поверхностной концентрации ионов, каталитической акт...

Интеграция режима сканирующей капиллярной микроскопии в сканирующие зондовые микроскопы открывает новые возможности в области управления локальными химическими реакциями. Implementation of the scanning capillary microscopy mode in the scanning probe microscopes opens new features in the control of local chemical reactions.

The development of scanning probe microscopy has allowed to solve a number of complex tasks of moving and handling of objects with nanometer precision. This possibility is in demand in the modern areas of precision materials processing. Developed multi-axis machining center allows us to achieve high-precision positioning and is characterized by low...

В настоящей работе приводится описание устройства разрабатываемого биосенсора на основе микроэлектромеханической системы для обнаружения вируса гриппа А. Приведены экспериментальные данные по использованию биочипа. Проведен теоретический расчет чувствительности разрабатываемого биосенсора. Получены изображения вируса гриппа А с помощью просвечивающ...

The morphology and thermal and mechanical properties of siloxane urethane block copolymers based on oligosiloxane- and oligoalkylene diols are investigated. The dependence of morphology on the conditions of synthesis is studied via atomic force microscopy and scanning electron microscopy.

Visualization of the structure of biological objects plays a key role in medicine, biotechnology, nanotechnology and IT-technology. Atomic force microscopy (AFM) is a promising method of studying of objects' morphology and structure. In this work, AFM was used to determine the size and shape of the bacterial strains of Escherichia coli M-17 and vis...

Thermomechanical properties of microactuators with shape-memory effect (SME) based on the Ti2NiCu/Pt layered composite are analyzed. Atomic-force microscopy is used to experimentally determine rigidity of the composite microactuators that represent cantilevers with sizes of 50 × 2 × 1 μm and a thickness of the SME active alloy layer of 0.5 μm in a...

Diverse morphology of aggregates of amyloidogenic proteins has been attracting much attention in the last few years, and there is still no complete understanding of the relationships between various types of aggregates. In this work, we propose the model, which universally explains the formation of morphologically different (wormlike and rodlike) a...

The aim of the study was the development of a biosensor for prediction and early detection of diseases, including myocardial infarction, based on the analysis of human saliva composition with implanted biosensor devices. For the implementation of the stated objective a device enabling real-time detection of small concentrations of proteins in speci...

We genetically modified tobacco mosaic virus (TMV) to surface display a characterized peptide with potent metal ion binding and reducing capacity (MBP TMV), and demonstrate that unlike wild type TMV, this construct can lead to the formation of discrete 10–40 nm gold nanoparticles when mixed with 3 mM potassium tetrachloroaurate. Using a variety of...