Ignacio Fernandez-Pastor

Fundación MEDINA · Department of Chemistry

Three procedures have been investigated for the isolation of tyrosol (1) and hydroxytyrosol (2) from a phenolic extract obtained from the solid residue of olive milling. These three methods, which facilitated the recovery of these phenols, were chemical or enzymatic acetylation, benzylation, and carbomethoxylation, and subsequent carbonylation or a...

Introduction: L‐Dopa, a key neurotransmitter used to treat neural disorders such as Parkinson's disease, is found in the seeds of the genus Mucuna at a sufficient concentration for possible commercial use. Objective: To develop a simple and reliable method to extract L‐Dopa from M. pruriens seeds in an aqueous medium and then quantitate this compou...

Microwave-assisted extraction is compared with a more classical technique, Soxhlet extraction, to determine the content of triterpene acids in olive skins. The samples used in their original unmilled state and milled were extracted with ethyl acetate or methanol as solvents. The optimized operating conditions (e.g. amount and type of solvent, and t...

Current needs in finding new antibiotics against emerging multidrug-resistant superbugs are pushing the scientific community into coming back to Nature for the discovery of novel active structures. Recently, a survey of halophilic actinomyectes from saline substrates of El Saladar del Margen, in the Cúllar-Baza depression (Granada, Spain), led us t...

El género Kribbella, descrito en 1999, constituye un potencial productor de nuevos compuestos antimicrobianos. Miembros de este género han sido ya descritos como productores de metabolitos muy potentes contra Staphylococcus aureus resistente a meticilina (MRSA) como la sandramicina, un depsipéptido aislado por primera vez de un cultivo de Nocardioi...

A novel cyclic antimalarial and antitrypanosome hexapeptide, pipecolisporin (1), was isolated from cultures of Nigrospora oryzae CF-298113, a fungal endophyte isolated from roots of Triticum sp. collected in a traditional agricultural land of Montefrío, Granada, Spain. The structure of this compound, including its absolute configuration, was elucid...

Terpenoids, phenolic compounds and alkaloids are examples of some secondary metabolites responsible in how plants interact with their environment. These compounds present a wide diversity of structures and functional groups, which imply several biological activities. The metabolic pathways which control its biogenesis could be easily modified by am...

PEGylation defines the modification of a protein, peptide or non-peptide molecule by the linking of one or more polyethylene glycol (PEG) chains. The PEG-molecule conjugates have several advantages: a prolonged residence in body, a decreased degradation by metabolic enzymes and a reduction or elimination of protein immunogenicity. This versatile te...