Ignacio Fernández Monzón

Ignacio Fernández Monzón
Instituto Español de Oceanografia | IEO · Centro Oceanográfico de Vigo

PhD

About

63
Publications
27,949
Reads
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1,209
Citations
Introduction
Ignacio Fernández currently works at Centro Oceanográfico de Vigo, from the Instituto Español de Oceanografía. He has a Bachelor degree in Marine Sciences, a Master in Aquaculture and a PhD in Biology. He does research in fish nutrition and early development applying multidisciplinar and integrative approaches.
Additional affiliations
December 2021 - present
Instituto Español de Oceanografia
Position
  • Researcher
July 2018 - December 2021
Instituto Tecnológico Agrario de Castilla y León, Spain
Position
  • Researcher
January 2012 - June 2018
Universidade do Algarve
Position
  • PhD
Education
September 2006 - June 2008
September 2006 - June 2011
September 1996 - February 2004
University of Vigo
Field of study

Publications

Publications (63)
Article
Aquaculture growth and sustainability mainly rely on the identification and implementation of alternative raw materials to replace fish meal (FM) and fish oil (FO) and/or its major substitute, the soybean meal (SBM). A five-step screening tool has been designed to identify and improve the use of promising alternative raw materials. To validate it,...
Article
The marine habitat and its biodiversity can be impacted by released pharmaceuticals. The short-term (7 days) effect of 3 commonly used drugs – warfarin, dexamethasone and imidazole – on Senegalese sole (Solea senegalensis) juveniles was investigated. Occurrence of hemorrhages, histopathological alterations, antioxidant status, activity of antioxida...
Article
Full-text available
In ectotherm species, environmental temperature plays a key role in development, growth, and survival. Thus, determining how temperature affects fish populations is of utmost importance to accurately predict the risk of climate change over fisheries and aquaculture, critical to warrant nutrition and food security in the coming years. Here, the pote...
Book
Cellular and Molecular Approaches in Fish Biology is a highly interdisciplinary resource to bring industry professionals, students and researchers up-to-date with the latest developments and information on fish biology research combining a historical overview of the different research areas in fish biology and detailed descriptions of cellular and...
Chapter
Noncoding RNAs (ncRNAs) play a central role in the complex and tightly regulated processes sustaining life, from gene transcription to posttranscriptional regulation, including DNA methylation. Among the different ncRNAs, microRNAs (miRNAs) are the most extensively studied and characterized. Here, we will briefly describe their biogenesis and mecha...
Article
Catfish (Siluriformes) are important species for aquaculture worldwide, with an annual production in 2018 of ca. 6 million t. This review focuses on reassessing larval development, first feeding, and early rearing practices of the most important farmed catfish species, along with some candidate species for aquaculture diversification: Pangasianodon...
Article
Pharmaceuticals represent a group of emerging contaminants. The short-term effect (3 and 7 days) of warfarin (1 and 10 mg L⁻¹), dexamethasone (0.392 and 3.92 mg L⁻¹) and imidazole (0.013 and 0.13 mg L⁻¹) exposure was evaluated on mussels (Mytilus galloprovincialis). Total antioxidant status, glutathione reductase, glutathione peroxidase (GPx) and s...
Article
Full-text available
Skeletal deformities reduce fish viability, growth, wellbeing, and feed efficiency but also degrade the consumer’s perception of aquaculture products. Herein, the skeletal development and the incidence of skeletal deformities in tench (Tinca tinca) reared in semi-extensive conditions has been described in detail for the first time. Larval skeletons...
Article
Full-text available
Kisspeptin regulates puberty and reproduction onset, acting upstream of the brain-pituitary-gonad (HPG) axis. This study aimed to test a kisspeptin-based hormonal therapy on cultured Senegalese sole (G1) breeders, known to have reproductive dysfunctions. A single intramuscular injection of KISS2-10 decapeptide (250 µg/kg) was tested in females and...
Article
Full-text available
Aquaculture's sustainability deeply relies on the identification and inclusion of alternative raw materials. Although meals from insects and/or byproducts from different industries are being recently tested, the meal from terrestrial vegetable species is still the main substitution candidate for fish meal. Here the effects of 0% (Control), 10% (A10...
Article
Full-text available
Vitamin K (VK) is a fat-soluble vitamin that vertebrates have to acquire from the diet, since they are not able to de novo synthesize it. VK has been historically known to be required for the control of blood coagulation, and more recently, bone development and homeostasis. Our understanding of the VK metabolism and the VK-related molecular pathway...
Article
Full-text available
Vitamin K (VK) is a key nutrient for several biological processes (e.g., blood clotting and bone metabolism). To fulfill VK nutritional requirements, VK action as an activator of pregnane X receptor (Pxr) signaling pathway, and as a co-factor of γ-glutamyl carboxylase enzyme, should be considered. In this regard, VK recycling through vitamin K epox...
Poster
The kiss system is known to regulate puberty and to be involved in the onset of reproduction, acting upstream of the Brain-Pituitary-Gonad axis in gonadotrophin releasing hormone neurons and stimulating secretion of GnRH. Kisspeptin has been proven to be a novel therapeutic for fertility disorders in humans. In fish, positive results have also obse...
Article
Warfarin is the most worldwide used anticoagulant drug and rodenticide. Since it crosses placental barrier it can induce warfarin embryopathy (WE), a fetal mortality in neonates characterized by skeletal deformities in addition to brain hemorrhages. Although the effects of warfarin exposure in aquatic off target species were already described, the...
Article
Background: Vitamin K (VK) is a fat-soluble vitamin known for its essential role in blood coagulation, but also on other biological processes (e.g. reproduction, brain and bone development) have been recently suggested. Nevertheless, the molecular mechanisms behind its particular function on reproduction are not yet fully understood. Methods: Th...
Article
Farmed Senegalese sole (Solea senegalensis) still show a high incidence of vertebral anomalies that limit its intensive production and hamper its economic profitability. A great effort towards understanding how fish species develops and grows in captivity has been undertaken in the last decade, with particular emphasis to how different biotic and a...
Article
1. Temperature has a key impact on the physiology, food acquisition, metabolic rate, growth, and reproduction of organisms. Thus, exploring the effects of climate change on species physiology is important for the conservation and management of native and non-native bivalve species. 2. In this work, an integrative laboratory approach was used to co...
Chapter
Fat-soluble vitamins play essential roles in vertebrate’s development and homeostasis, and thus, an optimal, efficient and sustainable fish farming deeply depends on the optimization of their dietary levels provided. Subsequently, nutritional imbalances are considered one of the major causative factors of vertebrate’s abnormal development. Although...
Article
Fat-soluble vitamins play essential roles in vertebrate's development and homeostasis, and thus, an optimal, efficient and sustainable fish farming deeply depends on the optimization of their dietary levels provided. Subsequently, nutritional imbalances are considered one of the major causative factors of vertebrate's abnormal development. Although...
Chapter
Full-text available
During the early ontogeny of fish a variety of characteristics are determined as a response to environmental conditions and genetic background. As a lot of these characters are directly or indirectly related with the quality of on-growing fish, there is an urgent need to develop appropriate indices for the description and prediction of fish quality...
Article
Environmental toxicology has been expanding as growing concerns on the impact of produced and released chemical compounds over the environment and human health are being demonstrated. Among the toxic effects observed in organisms exposed to pollutants, those affecting skeletal tissues (osteotoxicity) have been somehow overlooked in comparison to he...
Chapter
Full-text available
The optimization of industrial production would only be possible with the discovery, identification and characterization of biological processes in which a nutrient or any other factor acts, as well as when their genes and genetic networks revealed. With the advent of Next Generation-Sequencing technologies, the discovery of non-coding RNAs having...
Chapter
Full-text available
Las investigaciones con las especies del género Seriola van en aumento por la importancia económica que representan para varias regiones del planeta. Mediante las herramientas genómicas, el grupo de Fisiología y Genómica Funcional del CIBNOR en colaboración con otras instituciones, están desarrollando diferentes investigaciones que permitirán conoc...
Article
The pregnane X receptor (PXR) is a nuclear receptor belonging to the NR1i sub-family and a known master regulator of xenobiotic metabolism. New roles have been recently proposed in mammals through its activation by vitamin K (VK) such as regulation of glucose metabolism, bone homeostasis, reproduction, neuronal development and cognitive capacities....
Article
Full-text available
Vitamin A (VA) and retinoid derivatives are known morphogens controlling vertebrate development. Despite the research effort conducted during the last decade, the precise mechanismof how VA induces post-natal bone changes, and particularly those operating through crosstalk with the thyroid hormones (THs) remain to be fully understood. Since effects...
Article
The natural variation of environmental factors in freshwater basins determines their biodiversity. Among them, calcium is a key physiological compound for freshwater invertebrates. It is required for shell formation, muscle contraction, it mediates gene expression and allows counteracting acidosis during stress periods, among other functions. Altho...
Article
Full-text available
The ability of zebrafish to fully regenerate its caudal fin has been explored to better understand the mechanisms underlying de novo bone formation and to develop screening methods towards the discovery of compounds with therapeutic potential. Quantifying caudal fin regeneration largely depends on successfully measuring new tissue formation through...
Article
BMP2, BMP4 and BMP16 form a subfamily of bone morphogenetic proteins acting as pleiotropic growth factors during development and as bone inducers during osteogenesis. BMP16 is the most recent member of this subfamily and basic data regarding protein structure and function, and spatio-temporal gene expression is still scarce. In this work, insights...
Article
Senegalese sole (Solea senegalensis) has been considered since the 1990´s to be a promising flatfish species for diversifying European marine aquaculture. However, pathogen outbreaks leading to high mortality rates can impair Senegalese sole commercial production at the weaning phase. Different approaches have been shown to improve fish immunocompe...
Chapter
Full-text available
Vitamin K (VK) is a fat soluble vitamin required in fish diets for normal growth and development since they are not able to synthesize it de novo. VK can be naturally found as phylloquinone (VK1; vegetable origin) or menaquinone (VK2; microbial or animal origin); and synthetically produced as menadione (VK3). Two major roles of VK metabolites have...
Article
Vitamin K (VK) acts as a cofactor driving the biological activation of VK-dependent proteins and conferring calcium-binding properties to them. As a result, VK is converted into VK epoxide, which must be recycled by VK epoxide reductases (Vkors) before it can be reused. Although VK has been shown to play a central role in fish development, particul...
Data
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Article
Warfarin-induced vitamin K (VK) recycling impairment is used worldwide as a rodenticide and human thromboembolic prophylactic. Since VK metabolism/signaling pathways have been conserved throughout vertebrate evolution, its release to the environment might impact on aquatic organisms. Present study assessed the toxic effect of warfarin (0, 5, 25 and...
Article
Bone morphogenetic proteins (BMPs) are involved in various physiological processes from early life stages throughout adulthood. Specific characteristics of BMPs have been used to define different subfamilies and BMP2/4 subfamily (composed of BMP2 and BMP4) has been linked to osteogenesis and skeleton development. BMP16 was recently identified as a...
Article
Full-text available
This study provides a comprehensive description of the tissue organization of non- deformed and deformed opercula and vertebrae from gilthead sea bream Sparus aurata juveniles by means of histological, histochemical and immunohistochemical approaches. Two types of oper- cular anomalies are described: the folding of the opercle and subopercle into t...
Article
Nutritional factors strongly influence fish larval development and skeletogenesis, and may induce skeletal deformities. Vitamin K (VK) has been largely disregarded in aquaculture nutrition, despite its important roles in bone metabolism, in γ-carboxylation of Gla proteins, and in regulating gene expression through the pregnane X receptor (Pxr). Sin...
Chapter
Full-text available
This book is comprised of 9 chapters focusing on the diseases and disorders of cage cultured finfish. Topics discussed include an overview of cage culture and its importance in the 21st century, infectious diseases of coldwater fish in marine and brackish waters, infectious diseases of coldwater fish in fresh water, non-infectious disorders of cold...
Book
Biology of European Seabass presents up-to-date reviews on key topics of seabass biology, written by leading scientific experts with extensive knowledge of seabass as well as their respective field of expertise. The book covers the biology and ecology of the different sea basses and the latest findings in molecular biology, physiology, and beh
Article
Retinoic acid (RA), the main active metabolite of vitamin A, regulates vertebrate morphogenesis through signaling pathways not yet fully understood. Such process involves the specific activation of retinoic acid and retinoid X receptors (RARs and RXRs), which are nuclear receptors of the steroid/thyroid hormone receptor superfamily. Teleost fish ar...
Article
Full-text available
Abnormal pigmentation of Senegalese sole has been described as one problem facing the full exploitation of its commercial production. To improve our understanding of flatfish pigmentation of this commercially important species we have evaluated eleven genes related to two different processes of pigmentation: melanophore differentiation, and melanin...
Chapter
Substantial progress in pisciculture has been made in the last two decades, but many unidentified parameters that can still affect juvenile quality and, therefore, limit the costeffectiveness of the production represent an industrial bottleneck. The most critical phase of the whole production cycle is probably the larval period. To control this pha...
Article
Vertebral deformities constitute a recurrent problem in farmed teleost fish species. Potential causes of skeletal deformities are associated with genetic, environmental and nutritional factors. Considering nutritional factors, previous studies have shown that nutritional vitamin A (VA) imbalance implied the appearance of vertebral deformities in ma...
Article
In this study, we have evaluated the incorporation of two types of protein hydrolysates at 9 and 12% levels of inclusion, one from yeast (Saccharomyces cerevisiae, YPH) and another one from pig blood (PBPH), in microdiets for gilthead sea bream (Sparus aurata) larvae, and compared these results to a microdiet containing fish protein hydrolysate and...
Article
Flatfish can provide an excellent model to study developmental alterations of cartilage and bone tissues in response to vitamin A (VA), and therefore, with respect to the underlying morphogenesis, cell differentiation and proliferation processes, and gene expression regulation. Bone and cartilage development and homeostasis at different life stages...
Article
In bay snook (Petenia splendida) larvae the histological development of the digestive system and swim bladder, and their relative timing of differentiation were studied from hatching to 45days post-hatch (dph) at 29°C. Newly hatched larvae showed a simple digestive tract, which appeared as a straight undifferentiated tube lined by a single layer of...
Article
The effects of prolonged feed deprivation (40 days at 18° C) and re-feeding (30 days) on body mass, growth and the activity of selected pancreatic and intestinal enzymes were evaluated in migrating European glass eels Anguilla anguilla by comparison with a control group fed to satiation with hake Merluccius merluccius roe for the duration of the ex...
Article
Full-text available
Vitamin A (VA) has a key role in vertebrate morphogenesis, determining body patterning and growth through the control of cell proliferation and differentiation processes. VA regulates primary molecular pathways of those processes by the binding of its active metabolite (retinoic acid) to two types of specific nuclear receptors: retinoic acid recept...
Article
Summary Several studies have evaluated the effects of dietary vitamin A (VA) on the incidence of skeletal deformities during early ontogeny of fish, but little is known about its effects on bones depending on their process of ossification (dermal or chondral). We examined the incidence of skeletal deformities along development (30 and 48 dph) by do...
Article
The effects of different levels of vitamin A (VA) in Senegalese sole larval performance and development were evaluated by means of a dietary dose–response experiment using enriched Artemia metanauplii as a carrier of this micronutrient. Larvae were fed from 6 to 27 days post hatch (dph) with enriched Artemia containing graded levels of total VA (1....
Article
The digestive physiology of common dentex was studied by assessing the activity of different pancreatic (trypsin, chymotrypsin, amylase and lipase), intestinal (alkaline phosphatase, aminopeptidase N, maltase and leuncine–alanine peptidase) and gastric (pepsin) enzymes from hatching until the juvenile stage (50 dph at 19 °C). Enzymes involved in th...
Article
Several nutritional studies have found a direct effect of several vitamins in chondrogenic and osteogenic development during early life stages of marine fish species. In the present study, the effect of vitamin A (VA) in gilthead sea bream skeletogenesis was evaluated by means of four different dietary regimes (enriched rotifers) containing increas...
Article
The rhabdovirus viral haemorrhagic septicemia virus (VHSV) is the etiological agent of one of the most important salmonid viral diseases. In the present work, the ability of VHSV to infect and replicate in zebrafish at low temperature (15 degrees C) was demonstrated. Zebrafish was also used to determine the effectiveness of the recombinant virus rI...

Questions

Question (1)
Question
I would like to evaluate the oxidative stress in some samples. Literature review gives me two options: MDA or TBARS Assay. It seems that TBARS assay is not so specific as MDA since TBARS assay measures compounds other than MDA. This is right? Should I use MDA assay to quantify oxidative stress?

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Projects (6)
Project
This Special Issue aims to present and compile recent insights on how vitamins act at cellular and molecular level and how other factors and/or their own interaction might determine their influence on bone tissue, leading to normal or unhealthy bone phenotypes. Original research and reviews with fundamental or applied perspectives on how vitamins impact bone development and health are welcome, especially those using innovative and integrative approaches. Some discounts on APC fee might be applied, just send your tentative title and abstract to nacfm@hotmail.com.
Project
Fish growth potential, immunocompetence, reproductive performance and quality are determined in response to nutritional regimes, among other factors. Since these characters are directly or indirectly related with the production efficiency in on-growing fish, there is an urgent need to develop appropriate indices/biomarkers, not only for the correct diagnosis/description of the nutritional status of the farmed fish, but also for the early prediction of fish quality/physiological condition. In MET2VI, innovative, integrative and less invassive metodologies will be explored applying multidisciplinary approaches by an interdiciplinar team in order to discover how nutrients interact at different molecular, cellular, tissular and organismal level. Transcriptomics, proteomics and metabolomics will be combined to identify suitable earlier biomarkers to boost sustainability of farmed fish and enterprise competitiveness. Identified bimarkers will represent a key decision-making procedure for farm managers and feed companies.