Ignacio Bordeu

University of Cambridge | Cam · Department of Applied Mathematics and Theoretical Physics

The development of the mouse salivary gland involves a tip-driven process of branching morphogenesis that takes place in concert with differentiation into acinar, myoepithelial, and ductal (basal and luminal) sub-lineages. By combining clonal lineage tracing with a three-dimensional (3D) reconstruction of the branched epithelial network and single-...

Although melanoma is notorious for its high degree of heterogeneity and plasticity1,2, the origin and magnitude of cell-state diversity remains poorly understood. Equally, it is unclear whether growth and metastatic dissemination are supported by overlapping or distinct melanoma subpopulations. Here, by combining mouse genetics, single-cell and spa...

Human cholangiocyte organoids show great promise for regenerative therapies and in vitro modeling of bile duct development and diseases. However, the cystic organoids lack the branching morphology of intrahepatic bile ducts (IHBDs). Here, we report establishing human branching cholangiocyte organoid (BRCO) cultures. BRCOs self-organize into complex...

Cell signaling is essential for cell fate determination and tissue patterning. As signaling ligands are presented to the receiving cell, they are recruited and recognized by the cell membrane so as to elicit a biological response and to pattern multicellular tissues. Cells can accumulate and transport these ligands, which results in an emergent org...

Significance Understanding how intracellular signaling cascades control cell fate is a key issue in stem cell biology. Here we show that exit from the stem cell compartment in mammalian epidermis is characterized by pulsatile ERK MAPK activity. Basal activity and pulses are differentially regulated by DUSP10 and DUSP6, two phosphatases that have be...

Fluctuation in signal transduction pathways is frequently observed during mammalian development. However, its role in regulating stem cells has not been explored. Here we tracked spatiotemporal ERK MAPK dynamics in human epidermal stem cells. While stem cells and differentiated cells were distinguished by high and low stable basal ERK activity, res...

Branching processes are used to model diverse social and physical scenarios, from extinction of family names to nuclear fission. However, for a better description of natural phenomena, such as viral epidemics in cellular tissues, animal populations and social networks, a spatial embedding—the branching random walk (BRW)—is required. Despite its wid...

Branching processes are used to model diverse social and physical scenarios, from extinction of family names to nuclear fission. However, for a better description of natural phenomena, such as viral epidemics in cellular tissues, animal populations and social networks, a spatial embedding---the branching random walk (BRW)---is required. Despite its...

One of the key characteristics of multicellular organisms is the ability to establish and maintain shapes, or morphologies, under a variety of physical and chemical perturbations. A quantitative description of the underlying morphological dynamics is a critical step to fully understand the self-organising properties of multicellular systems. Althou...

Patches of vegetation consist of dense clusters of shrubs, grass, or trees, often found to be circular characteristic size, defined by the properties of the vegetation and terrain. Therefore, vegetation patches can be interpreted as localized structures. Previous findings have shown that such localized structures can self-replicate in a binary fash...

Desertification due to climate change and increasing drought periods is a worldwide problem for both ecology and economy. Our ability to understand how vegetation manages to survive and propagate through arid and semiarid ecosystems may be useful in the development of future strategies to prevent desertification, preserve flora-and fauna within-or...

A wide range of physical systems exhibit labyrinthine patterns as transient or steady states. One of the routes by which labyrinths emerge is through the destabilization of a localized structure through a curvature instability. In this work, we will study the emergence of labyrinths through this mechanism in different physical contexts, including n...

Macroscopic systems subjected to external forcing exhibit complex spatiotemporal behaviors as result of dissipative self-organization. We consider a paradigmatic Swift-Hohenberg equation in both variational and nonvariational forms in two-dimensions. We investigate in both equations the occurrence of a curvature instability generating spot multipli...

Stationary two-dimensional localized structures have been observed in a wide variety of dissipative systems. The existence, stability properties, dynamical evolution, and bifurcation diagram of an azimuthal symmetry breaking, rodlike localized structure in the isotropic prototype model of pattern formation, the Swift-Hohenberg model, is studied. Th...

The stability of a circular localized spot with respect to azimuthal perturbations is studied in in a variational Swift-Hohenberg model equation. The conditions under which the circular shape undergoes an elliptical deformation that transform it into a rod shape structure are analyzed. As it elongates the rod-like structure exhibits a transversal i...

In this paper, a comprehensive spatio-spectral and temporal analysis for Chromobacterium violaceum colonies is reported. A hyperspectral imaging (HSI) system is used to recover the spectral signatures of pigment production in a non-homogeneous media with high spectral resolution and high sensitivity in vivo, without destructing the sample. This non...

The study of diffusion in a crowded and complex environment, such as inside a cell or within a porous medium, is of fundamental importance for science and technology. Combining blinking holographic optical tweezers and sub-pixel video microscopy permits one to study Brownian motion in confined geometries. In this work, in particular, we have studie...