Ian Probert

Ian Probert
Station Biologique de Roscoff · RCC

About

266
Publications
92,678
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10,248
Citations
Additional affiliations
September 2011 - present
Sorbonne Université
Position
  • Ingénieur de Recherche

Publications

Publications (266)
Article
Coccolithophores are a calcifying unicellular phytoplankton group that are at the base of the marine food web, and their lipid content provides a source of energy to consumers. Coccolithophores are vulnerable to ocean acidification and warming, therefore it is critical to establish the effects of climate change on these significant marine primary p...
Article
Full-text available
In every liter of seawater there are between 10 and 100 billion life forms, mostly invisible, called marine plankton or marine microbiome, which form the largest and most dynamic ecosystem on our planet, at the heart of global ecological and economic processes. While physical and chemical parameters of planktonic ecosystems are fairly well measured...
Article
Full-text available
Biogeographical studies have traditionally focused on readily visible organisms, but recent technological advances are enabling analyses of the large-scale distribution of microscopic organisms, whose biogeographical patterns have long been debated. Here we assessed the global structure of plankton geography and its relation to the biological, chem...
Article
Planktonic protists are an essential component of marine pelagic ecosystems where they mediate important trophic and biogeochemical functions. Although these functions are largely influenced by their taxonomic affiliation, the composition and spatial variability of planktonic protist communities remain poorly characterized in vast areas of the ocea...
Preprint
The calcite platelets of coccolithophores (Haptophyta), the coccoliths, are among the most elaborate biomineral structures. How these unicellular algae accomplish the complex morphogenesis of coccoliths is still largely unknown. It has long been proposed that the cytoskeleton plays a central role in shaping the growing coccoliths. Previous studies...
Article
Heterococcoliths are calcite platelets produced inside diploid coccolithophore cells and extruded to form a covering on the cell surface called a coccosphere. The size of coccoliths is an important parameter sometimes used to identify species, and it is observed to be influenced in extant species by abiotic parameters (e.g., CO2, light). However, t...
Preprint
Full-text available
Planktonic protists are an essential component of marine pelagic ecosystems where they mediate important trophic and biogeochemical functions. Although these functions are largely influenced by their taxonomic affiliation, the composition and spatial variability of planktonic protist communities remain poorly characterized in vast areas of the ocea...
Preprint
Coccoliths are calcite platelets produced inside coccolithophore cells and extruded to form a covering on the cell surface called a coccosphere. The size of coccoliths is an important parameter often used to identify species, and observations on extant species have shown an influence of abiotic parameters (e.g., CO2, light, nutrient concentration)...
Article
Full-text available
Rapidly accumulating genetic data from environmental sequencing approaches have revealed an extraordinary level of unsuspected diversity within marine phytoplankton,1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 which is responsible for around 50% of global net primary production.¹²,¹³ However, the phenotypic identity of many of the organisms distinguished by e...
Article
Full-text available
The development of calcification by the coccolithophores had a profound impact on ocean carbon cycling, but the evolutionary steps leading to the formation of these complex biomineralised structures are not clear. Heterococcoliths consisting of intricately‐shaped calcite crystals are formed intracellularly by the diploid life cycle phase. Holococco...
Chapter
Cryopreservation has been successfully used in the banking and maintenance of cultures of microorganisms, from bacteria to yeasts, since the onset of cryobiology. Biobanking of marine biological resources is crucial for development of scientific knowledge as researchers rely on guaranteed access to reliable, stable resources. Culture collections pl...
Article
Full-text available
The haptophyte genus Pseudohaptolina (formerly Chrysochromulina clade B1-3) currently harbours two species: Pseudohaptolina arctica and Pseudohaptolina sorokinii. In addition, Chrysochromulina birgeri is expected to belong to this genus due to its morphological similarity to P. sorokinii, but it has not yet been genetically characterised. A strain...
Article
Organic nutrients can constitute the major fractions (up to 70%) of aquatic nitrogen (N) and phosphorus (P), but their cycling is poorly understood relative to the inorganic pools. Some phytoplankton species access P from the dissolved organic phosphorus (DOP) pool through expression of alkaline phosphatase (AP), which hydrolyses orthophosphate fro...
Preprint
Full-text available
In every liter of seawater there are between 10 and 100 billion life forms, mostly invisible, called plankton, which form the largest and most dynamic ecosystem on our planet, at the heart of global ecological and economic processes. While physical and chemical parameters of planktonic ecosystems are fairly well measured and modelled at the planeta...
Article
Full-text available
Viruses are recognised as the most abundant biological entities on the planet. In addition to their role in disease, they are crucial components of co-evolutionary processes, are instrumental in global biogeochemical pathways such as carbon fluxes and nutrient recycling, and in some cases act regionally on climate processes. Importantly, viruses ha...
Preprint
Full-text available
The haptophyte genus Pseudohaptolina (formerly Chrysochromulina clade B1-3) currently harbors two species: Pseudohaptolina arctica and Pseudohaptolina sorokinii . In addition, Chrysochromulina birgeri is expected to belong to this genus due to its morphological similarity to P. sorokinii , but has not yet been genetically characterized. A strain be...
Article
Full-text available
Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied, including flow cytometry cell sorting, manual single cell pipetting, and...
Article
Full-text available
The Green Edge initiative was developed to investigate the processes controlling the primary productivity and fate of organic matter produced during the Arctic phytoplankton spring bloom (PSB) and to determine its role in the ecosystem. Two field campaigns were conducted in 2015 and 2016 at an ice camp located on landfast sea ice southeast of Qikiq...
Chapter
Dinoflagellates are an extremely diverse group of unicellular eukaryotes that represent an important part of marine plankton communities. Among other secondary metabolites, these microorganisms are known to produce toxins such as the saxitoxins, karlotoxins, amphidinolides, okadaic acid or brevetoxins, which are extremely toxic for marine fauna and...
Poster
Full-text available
Abstract: The Deep Biomass (DB), located in the lowest part of the euphotic zone (approx. 80 m to 300 m) primarily in the subtropical gyres is an unexplored part of primary production (PP). It is one of the largest biomes on our planet considering that subtropical regions occupy 60% of the total ocean. Recent observations indicate that the major oc...
Preprint
Full-text available
Biogeographical studies have traditionally focused on readily visible organisms, but recent technological advances are enabling analyses of the large-scale distribution of microscopic organisms, whose biogeographical patterns have long been debated 1,2 . The most prominent global biogeography of marine plankton was derived by Longhurst ³ based on p...
Article
Biopolymers, such as exopolysaccharides are widely exploited by industry as hydrocolloids (gelling, thickening agents) and biological agents (anti-inflammatory, anti-parasitic, antioxidant, etc.). In this study, 166 marine microalgae and cyanobacteria species have been screened in order to identify strains producing original exopolysaccharides. Thi...
Article
Full-text available
Microalgae and their metabolites have been an El Dorado since the turn of the 21st century. Many scientific works and industrial exploitations have thus been set up. These developments have often highlighted the need to intensify the processes for biomass production in photo-autotrophy and exploit all the microalgae value including ExoPolySaccharid...
Article
Full-text available
Phytoplankton account for nearly half of global primary productivity and strongly affect the global carbon cycle, yet little is known about the forces that drive the evolution of these keystone microscopic organisms. Here we combine morphometric data from the fossil record of the ubiquitous coccolithophore genus Gephyrocapsa with genomic analyses o...
Article
Full-text available
The Green Edge initiative was developed to investigate the processes controlling the primary productivity and the fate of organic matter produced during the Arctic phytoplankton spring bloom (PSB) and to determine its role in the ecosystem. Two field campaigns were conducted in 2015 and 2016 at an ice camp located on landfast sea ice southeast of Q...
Article
The chemical composition of five marine microalgae (Dunaliella sp., Dunaliella salina, Chaetoceros calcitrans, Chaetoceros gracilis and Tisochrysis lutea) was investigated through nuclear magnetic resonance (NMR) spectroscopic study of the soluble material obtained by sequential extraction with hexane, ethyl acetate (AcOEt) and methanol of biomass...
Article
Full-text available
Bacterial biofilms are complex biological systems that are difficult to eradicate at a medical, industrial, or environmental level. Biofilms confer bacteria protection against external factors and antimicrobial treatments. Taking into account that about 80% of human infections are caused by bacterial biofilms, the eradication of these structures is...
Preprint
Full-text available
Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied, including flow cytometry cell sorting, manual single cell pipetting and s...
Article
Full-text available
Coccolithophores of the Noëlaerhabdaceae family are covered by imbricated coccoliths, each composed of multiple calcite crystals radially distributed around the periphery of a grid. The factors that determine coccolith size remain obscure. Here, we used synchrotron-based three-dimensional Coherent X-ray Diffraction Imaging to study coccoliths of 7...
Data
Fig. S1 Images of internal malformed coccoliths. Fig. S2 Images of silicon (Si)‐depleted cultures. Fig. S3 Photosynthetic efficiency following disruption of calcification. Fig. S4 Time‐lapse microscopy of cell division in Coccolithus braarudii. Fig. S5 Cell division can occur in the absence of a coccosphere. Fig. S6 Malformed coccolith product...
Data
Video S1 Cell division in Coccolithus braarudii.
Article
Full-text available
Coccolithophores are globally distributed unicellular marine algae that are characterized by their covering of calcite coccoliths. Calcification by coccolithophores contributes significantly to global biogeochemical cycles. However, the physiological requirement for calcification remains poorly understood as non‐calcifying strains of some commonly...
Article
Full-text available
All characterized members of the ubiquitous genus Acaryochloris share the unique property of containing large amounts of chlorophyll (Chl) d, a pigment exhibiting a red absorption maximum strongly shifted towards infrared compared to Chl a. Chl d is the major pigment in these organisms and is notably bound to antenna proteins structurally similar t...
Article
Full-text available
Background : Study of meta-transcriptomic datasets involving non-model organisms represents bioinformatic challenges. The production of chimeric sequences and our inability to distinguish the taxonomic origins of the sequences produced are inherent and recurrent difficulties in de novo assembly analyses. As the study of holobiont meta-transcriptome...
Article
Dinoflagellates are one of the most abundant and functionally diverse groups of eukaryotes. Despite an overall scarcity of genomic information for dinoflagellates, constantly emerging high‐throughput sequencing resources can be used to characterize and compare these organisms. We assembled de novo and processed 46 dinoflagellate transcriptomes and...
Preprint
Full-text available
Background Study of meta-transcriptomic datasets involving non-model organisms represents bioinformatic challenges. The production of chimeric sequences and our inability to distinguish the taxonomic origins of the sequences produced are inherent and recurrent difficulties in de novo assembly analyses. The study of holobiont transcriptomes shares s...
Preprint
Full-text available
Dinoflagellates are one of the most abundant and functionally diverse groups of eukaryotes. Despite an overall scarcity of genomic information for dinoflagellates, constantly emerging high-throughput sequencing resources can be used to characterize and compare these organisms. We assembled de novo and processed 46 dinoflagellate transcriptomes and...
Chapter
Full-text available
Haptophyta are predominantly planktonic and phototrophic organisms that have their main distribution in marine environments worldwide. They are a major component of the microbial ecosystem, some form massive blooms and some are toxic. Haptophytes are significant players in the global carbonate cycle through photosynthesis and calcification. They ar...
Data
Marine phytoplankton are responsible for approximately 50% of global primary production and are at the base of marine food webs. In the Arctic Ocean, one important factor controlling phytoplankton productivity is sunlight, as sea-ice and snow cover limits light penetration in the water column. Over the last three decades, the extent of the seasonal...
Poster
Full-text available
Polar environments are undergoing dramatic changes, as the rapidly shrinking ice cover in the Arctic Ocean opens path for phytoplankton blooms profiting from the increased incident light. Green Edge is a multidisciplinary franco-canadien project, enrolling almost 20 institutions from both countries. Its main goal is to understand the dynamics of th...
Presentation
Full-text available
Polysaccharides which are composed of monosaccharides linked by glycosidic bonds are widely exploited by industry as hydrocolloids (gelling or thickening agents) and bioactives (e.g. anti-inflammatory, anti-parasitic or antioxidant agents). Microalgae are extremely diverse unicellular photosynthetic microorganisms that produce extracellular polysac...
Chapter
Full-text available
Haptophyta are predominantly planktonic and phototrophic organisms that have their main distribution in marine environments worldwide. They are a major component of the microbial ecosystem, some form massive blooms and some are toxic. Haptophytes are significant players in the global carbonate cycle through photosynthesis and calcification. They ar...