Hyeogsun Kwon

Hyeogsun Kwon
Iowa State University | ISU · Department of Entomology

Ph.D

About

46
Publications
5,647
Reads
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783
Citations
Additional affiliations
March 2019 - present
Iowa State University
Position
  • Researcher
November 2015 - present
Iowa State University
Position
  • PostDoc Position
May 2013 - October 2015
Texas A&M University
Position
  • PostDoc Position

Publications

Publications (46)
Preprint
Vector-borne, filarial nematode diseases represent a significant and affecting disease burden in humans, domestic animals, and livestock worldwide. Parasitic filarial nematodes require both an intermediate (vector) host and a definitive (mammalian) host during the course of their life cycle. In either host, the nematode must evade the host elicited...
Article
Full-text available
Characterizing the physiological changes that accompany malaria parasite infection of the mosquito host is crucial to our understanding of vectorial capacity in Anopheles mosquitoes, yet has not fully been explored. In this study, we examine the role of uric acid metabolism in the mosquito, Anopheles gambiae, following malaria parasite infection. W...
Article
Full-text available
Lipid-derived signaling molecules known as eicosanoids have integral roles in mediating immune and inflammatory processes across metazoans. This includes the function of prostaglandins and their cognate G protein-coupled receptors (GPCRs) to employ their immunological actions. In insects, prostaglandins have been implicated in the regulation of bot...
Article
Full-text available
Mosquito immune cells, known as hemocytes, are integral to cellular and humoral responses that limit pathogen survival and mediate immune priming. However, without reliable cell markers and genetic tools, studies of mosquito immune cells have been limited to morphological observations, leaving several aspects of their biology uncharacterized. Here,...
Article
Full-text available
Mosquitoes may feed multiple times during their life span in addition to those times needed to acquire and transmit malaria. To determine the impact of subsequent blood feeding on parasite development in Anopheles gambiae, we examined Plasmodium parasite infection with or without an additional noninfected blood meal. We found that an additional blo...
Article
Full-text available
The innate immune system is the primary defense response to limit invading pathogens for all invertebrate species. In insects, immune cells are central to both cellular and humoral immune responses, however few genetic resources exist beyond Drosophila to study immune cell function. Therefore, the development of innovative tools that can be widely...
Article
Full-text available
Mosquito physiology and immunity are integral determinants of malaria vector competence. This includes the principal role of hormonal signaling in Anopheles gambiae initiated shortly after blood-feeding, which stimulates immune induction and promotes vitellogenesis through the function of 20-hydroxyecdysone (20E). Previous studies demonstrated that...
Preprint
Full-text available
Lipid-derived signaling molecules known as eicosanoids have integral roles in mediating immune and inflammatory processes across metazoans. This includes the function of prostaglandins and their cognate G protein-coupled receptors (GPCRs) to employ their immunological actions. In insects, prostaglandins have been implicated in the regulation of bot...
Preprint
Full-text available
Several endocrine signals are known to mediate mosquito egg development including insulin-like peptide, 20-hydroxyecdysone, and juvenile hormone. The objective of this study was to determine the effects of prostaglandin E 2 (PGE 2 ) as an additional mediator of oogenesis in the mosquitoes, Aedes albopictus and Anopheles gambiae . The injection of a...
Preprint
Full-text available
Mosquito immune cells, known as hemocytes, are integral to cellular and humoral responses that limit pathogen survival and mediate immune priming. However, without reliable cell markers and genetic tools, studies of mosquito immune cells have been limited to morphological observations, leaving several aspects of their biology uncharacterized. Here,...
Article
Full-text available
Blood feeding is required to provide nutrients for mosquito egg production and serves as a mechanism to acquire and transmit pathogens. Shortly after a blood meal is taken, there is a peak in the production of 20-hydroxyecdysone (20E), a mosquito hormone that initiates physiological changes, including yolk protein production and mating refractorine...
Preprint
Full-text available
Blood-feeding is an integral behavior of mosquitoes to acquire nutritional resources needed for reproduction. This requirement also enables mosquitoes to serve as efficient vectors to acquire and potentially transmit a multitude of mosquito-borne diseases, most notably malaria. Recent studies suggest that mosquito immunity is stimulated following a...
Preprint
Full-text available
A minimum of two blood meals are required for a mosquito to acquire and transmit malaria, yet Anopheles mosquitoes frequently obtain additional blood meals during their adult lifespan. To determine the impact of subsequent blood-feeding on parasite development in Anopheles gambiae, we examined rodent and human Plasmodium parasite infection with or...
Article
Full-text available
Eicosanoids are bioactive signaling lipids derived from the oxidation of fatty acids that act as important regulators of immune homeostasis and inflammation. As a result, effective anti-inflammatory drugs have been widely used to reduce pain and inflammation which target key eicosanoid biosynthesis enzymes. Conserved from vertebrates to insects, th...
Article
Mosquito immunity is composed of both cellular and humoral factors that provide protection from invading pathogens. Immune cells known as hemocytes, have been intricately associated with phagocytosis and innate immune signaling. However, the lack of genetic tools has limited hemocyte study despite their importance in mosquito anti-Plasmodium immuni...
Article
Full-text available
Mosquito innate immunity is comprised of both cellular and humoral factors that provide protection from invading pathogens. Immune cells, known as hemocytes, have been intricately associated with these immune responses through direct roles in phagocytosis and immune signaling. Recent studies have implicated hemocytes as integral determinants of ant...
Article
Full-text available
Background There is emerging evidence that mosquito anti-Plasmodium immunity is multimodal with distinct mechanisms for killing malaria parasites at either the ookinete or oocyst stages. Early-phase responses targeting the ookinete require complement-like components circulating in the mosquito hemolymph that result in TEP1-mediated lysis or melaniz...
Article
Full-text available
Insect kinins (leucokinins) are multifunctional peptides acting as neurohormones and neurotransmitters. In females of the mosquito vector Aedes aegypti (L.), aedeskinins are known to stimulate fluid secretion from the renal organs (Malpighian tubules) and hindgut contractions by activating a G protein-coupled kinin receptor designated “Aedae-KR.” W...
Article
Full-text available
Ticks transmit more pathogens to humans and animals than any other arthropod. We describe the 2.1 Gbp nuclear genome of the tick, Ixodes scapularis (Say), which vectors pathogens that cause Lyme disease, human granulocytic anaplasmosis, babesiosis and other diseases. The large genome reflects accumulation of repetitive DNA, new lineages of retro-tr...
Article
Full-text available
Ticks transmit more pathogens to humans and animals than any other arthropod. We describe the 2.1 Gbp nuclear genome of the tick, Ixodes scapularis (Say), which vectors pathogens that cause Lyme disease, human granulocytic anaplasmosis, babesiosis and other diseases. The large genome reflects accumulation of repetitive DNA, new lineages of retro-tr...
Data
Supplementary Figures 1-25, Supplementary Tables 1-38, Supplementary Note 1, Supplementary Methods and Supplementary References
Article
In anautogenous mosquitoes such as Aedes aegypti females the calcitonin-like diuretic hormone 31 (DH31) stimulates natriuretic fluid excretion from the Malpighian tubules (MTs) after a blood meal. We previously cloned and functionally characterized AedaeGPCRcal1 from A. aegypti, the ortholog of the Drosophila melanogaster DH31 receptor and immunolo...
Article
Full-text available
Evolution of anthropophilic hematophagy in insects resulted in the coordination of various physiological processes for survival. In female mosquitoes, a large blood meal provides proteins for egg production and as a trade-off, rapid elimination of the excess water and solutes (Na(+), Cl(-)) is critical for maintaining homeostasis and removing exces...
Data
Full-text available
Amino acid sequence alignment of calcitonin receptor-like receptors. AaegGPRCAL1 was aligned with those of other arthropods, a mollusk, and vertebrates. The A. aegypti GPRCAL1(AEU12191)1 sequence is 79% identical to C. quinquefasciatus GPRCAL1 (CPIJ014419–PA)2, 75% identical to A. gambiae GPRCAL1 (AGAP009770-PA)3, 64% to sequenced D. melanogaster G...
Data
Full-text available
Phylogenetic tree showing evolutionary relationships for GPRCAL1. Corticotropin-releasing hormone 1-like receptors (CRHR1), CALCRs and CALCRLs from vertebrates and invertebrates were analyzed by the neighbor-joining method with bootstrap analysis of 10,000 replicates with MEGA5. A secretin type GPCR from Caenorhabditis elegans (NP_510496.1; WormBas...
Data
Primers used for cloning, transcriptional and functional analyses (RNAi) of AaegGPRcal1. (PDF)
Data
AaegGPRcal1 full length cDNA cloned from MTs, and deduced amino acid sequence. The cDNA sequence is 1995 bp, encoding a 412 amino acid residue protein. Seven transmembrane regions are predicted by TMHMM and underlined (•–•). The highly conserved six cysteine (C21, C40, C49, C63, C80, C102), two tryptophan (W50, W86), two proline (P51, P64), and asp...
Data
Full-text available
Immunolocalization of AaegGPRCAL1 along the length of a single MT. The receptor signal (red, white arrows) was observed in only particular principal cells, the majority located towards the distal end which contains the tip cell. (PDF)
Data
Full-text available
Localization of AaegGPRCAL1 in MTs from females injected with AaegGPRcal1 dsRNA, EGFP dsRNA and water. (A) MTs from AaegGPRcal1 knock-down females exhibited reduction of receptor signal intensity (in this image by a factor of 2) compared to those of controls (B) EGFP dsRNA and (C) water. (PDF)
Data
Full-text available
Amino acid sequence alignment of calcitonins (CTs) and calcitonin gene related peptides (CGRPs). (A) The A. aegypti diuretic hormone 31 (Aaeg-DH31) sequence was aligned with those of other arthropod DH31 and CTs and in (B) with CGRPs. In (A) and (B), GenBank accession numbers: 1. A. aegypti DH31 (EAT40182). 2. A. gambiae DH31 (XP_321755). 3. D. mel...
Conference Paper
In mosquitoes, the calcitonin-like diuretic hormone 31 (DH31), known as mosquito natriuretic peptide (MNP), increases sodium and fluid secretion. Although there are three predicted calcitonin receptor-like receptors (GPRCAL1-GPCRCAL3) in the Aedes aegypti genome, the specific contribution of each receptor to rapid fluid excretion in response to DH3...
Article
Every day, people and animals contract debilitating and life threatening diseases due to bites from infected flies, ticks, and mosquitoes. The current methods utilized to fight against these diseases are only partially effective or safe for humans and animals. When it comes to insect vector control, a conceptual paradigm shift is urgently needed. T...
Conference Paper
The biological role of calcitonin receptor-like receptors that belong to the family B of G-protein coupled receptors (GPCRs), in maintenance of water balance of females of mosquito Aedes aegypti is being studied. In Anopheles gambiae, the Angoga-DH31 receptor (a calcitonin receptor-like receptor, AaegGPRCal1), functions in maintenance of water bala...
Article
We report on experiments designed to more thoroughly document the roles of eicosanoids as crucial elements in cell spreading and on experiments designed to test the hypothesis that in vivo bacterial infections influence cell spreading on glass surfaces. We used hemocytes prepared from tobacco hornworms, Manduca sexta (L.) (Lepidoptera: Sphingidae)...
Article
Salivary enzyme, glucose oxidase (GOX) from the caterpillar Helicoverpa zea, catalyzes the conversion of glucose to gluconic acid and hydrogen peroxide. Because hydrogen peroxide has well-known antimicrobial properties, we examined whether caterpillar labial saliva could reduce the infectivity of bacterial pathogens. We examined the effects of cate...

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