Hussein Abulreesh

• PhD
• Faculty Member at Umm al-Qura University

Background Cosmetics have become essential for skincare, makeup, and hair care. Cosmetic products can be contaminated during production and application. This study investigated the staphylococci contamination parameters (virulence factors, diversity, and antimicrobial susceptibility patterns) in cosmetic products in Western Saudi Arabia. Materials...

For the first time, this study reports extracellular uricase enzyme isolation and characterization from strain UR1 of Alcaligenes spp. from Western Saudi Arabia. The strain efficiently produced highly active extracellular uricase for therapeutic applications. It offers a simplified enzyme purification approach rather than complicated intracellular...

Foodborne pathogens are often associated with pandemic, endemic, and sporadic foodborne disease outbreaks. These pathogenic bacteria commonly belong to genus Vibrio spp., Salmonella spp., Listeria monocytogens, Escherichia coli, Clostridium spp., Bacillus spp., Campylobacter spp., and Staphylococcus spp. Antimicrobials are the mainstay of infectiou...

Campylobacter spp. is a major source of global gastrointestinal infections. Their enteric infections are linked to the consumption of undercooked poultry products, contaminated milk and water, and the handling of wild animals and birds. The detection of Campylobacter spp. in water and food samples mainly depends on culture-based techniques. Public...

Aflatoxin B1 (AFB1) is a carcinogenic secondary metabolite of filamentous Aspergillus species. This study investigated the effects of Saudi Arabian (local) and Egyptian (imported) Aspergillus flavus-associated AFB1 on rats (hematological parameters, liver, and kidneys). Saudi Arabian and Egyptian A. flavus-associated AFB1 (0.5 mg/kg) were separatel...

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Salmonella spp. is an important foodborne pathogen and a leading cause of foodborne outbreaks worldwide. Shigella spp. is considered an important foodborne pathogen in low-income countries in Asia and Africa, where it is believed to be endemic in these regions. A broad range of selective plating and enrichment media have been formulated to detect a...

20 Staphylococcus haemolyticus is an emerging multidrug-resistant infectious agent 21 in Saudi Arabian clinical settings. During this study, urine specimens (1000) of 22 hospitalized and outpatients were screened for S. haemolyticus prevalence along with 23 antimicrobial resistance profiles and virulence factors. The primary identification of S. 24...

Milk, a nutritious global important food commodity, serves as an excellent medium for microbial growth as well. The foodborne pathogen Staphylococcus aureus is a commensal member of human microflora that enters the food chain through poor hygienic practices and cross contamination and causes various clinical manifestations in humans. During this st...

Saffron is well-known for its anti-apoptotic, anti-inflammatory, and antioxidant properties. Saffron's nutritional and medicinal properties support its numerous uses as a flavouring and herbal remedy. This study investigated the protective efficacy of saffron administration against aflatoxin B1 (AFB1)-induced toxicity in adult male Wistar albino ra...

The escalating incidence of bacterial resistance to commonly prescribed antibiotics underscores the urgent need for the rapid development of innovative antibacterial medications. Heterocyclic compounds, particularly nitrogen‐containing heterocycles like pyrazoles and thiazoles, have garnered attention for their diverse biological activities, includ...

The uricase enzyme yields allantoin, hydrogen peroxide, and carbon dioxide by catalyzing the oxidative opening of the purine ring in the urate pathway. This enzyme is important for biochemical diagnosis and reduces toxic urate accumulation during various diseases (hyperuricemia, gout, and bedwetting). Direct urate oxidase injection is recommended i...

The diverse environmental distribution of Salmonella makes it a global source of human gastrointestinal infections. This study aimed to detect Salmonella spp. and explore their diversity and antimicrobial susceptibility patterns in clinical and environmental samples. Pre-enrichment, selective enrichment, and selective plating techniques were adopte...

Multidrug resistance patterns of Acinetobacter spp. have led to their emergence as an important source of nosocomial infections. This study investigated the prevalence and clinical characteristics of Acinetobacter spp. in hospital-acquired wound and urinary tract infections. A total of 432 samples [wound swabs (210) and urine samples (222)] were an...

Microbial biopolymers have emerged as promising solutions for environmental pollution-related human health issues. Poly-γ-glutamic acid (γ-PGA), a natural anionic polymeric compound, is composed of highly viscous homo-polyamide of D and L-glutamic acid units. The extracellular water solubility of PGA biopolymer facilitates its complete biodegradati...

Pet reptiles are gaining popularity among Saudi citizens but owners lack public health-associated awareness. Generally, the pet shops do not properly guide about reptile handling and health precautions. This study features molecular characterization of Salmonella isolates from pet reptiles to detect potential human pathogenic serovars. Previously i...

Background: Aflatoxins are food-contaminating metabolites that can intoxicate and damage multiple organs. This study compared the effects of aflatoxin powder (AFB) and aflatoxin liquid (AFS) administrations on the testes and lungs of rats and elaborated their potential activity mechanisms.Methods: Fifteen rats were equally divided into 3 groups. Th...

Exopolysaccharides (EPS) are bioactive metabolites with high molecular weight and are produced by several microorganisms such as endophytic fungi. Thus, the aim of the present study was designed to explore the biosynthesis and characteristics of EPS by the endophytic fungus Neopestalotiopsis clavispora AUMC15969 and then investigate some environmen...

Background: Aflatoxin (AF), a metabolite of Aspergillus flavus, is injurious to vital body organs. The bacterial defense against such mycotoxins has attracted significant attention. Lactic acid bacteria (LAB) are known to ameliorate AF toxicity. Methods: Thirty adult male rats were divided into six groups (five each) to perform the experiments. The...

Fungal infections (mycoses) represent a major health issue in humans. They have emerged as a global concern for medical professionals by causing high morbidity and mortality. Fungal infections approximately impact one billion individuals per annum and account for 1.6 million deaths. The diagnosis of Candida infections is a challenging task. Laborat...

Hospital acquired-Staphylococcus aureus (HA-Staphylococcus aureus), particularly methicillin-resistant Staphylococcus aureus (MRSA), are an important source of nosocomial infections with high morbidity and mortality rates. Few reports showed that infections due to HA-Staphylococcus aureus in Saudi Arabia is increasing, particularly infections attri...

Download PDF Download PDF Article Open Access Published: 14 February 2023 Evaluation of synthesized biosurfactants as promising corrosion inhibitors and alternative antibacterial and antidermatophytes agents Ahmed Fawzy, Areej Al Bahir, …Khaled Elbanna Show authors Scientific Reports volume 13, Article number: 2585 (2023) Cite this article Metrics...

An efficient method using ZnO(NPs) was used to synthesize derivatives of s-substituted-1,2,4-triazoles with excellent yield. The structure of all newly formed triazoles was approved using the data extracted from their spectral and elemental analyses. Fifteen s-substituted-triazole derivatives were tested for their antimicrobial activity and the res...

Exopolysaccharides (EPSs) are novel functional additives for low-fat yogurt. Pharmaceutical, medical, and food industries are using more LAB-based EPSs. In this study, Leuconostoc spp. was used to produce ninth bacterial EPSs in a modified molasses medium. Production of EPSs was concentration-dependent on all stains and the highest yield was obtain...

Milk is a putrescible commodity that is extremely prone to microbial contamination. Primarily, milk and dairy products are believed to be easily contaminated by pathogenic microorganisms, including Listeria monocytogenes, Salmonella spp., and Staphylococcus aureus. The microbiological quality of raw milk and dairy products regarding foodborne patho...

Background: Urinary tract infections (UTIs) remain a common and leading cause of morbidity worldwide, with more than 150 million people affected annually, resulted in a social and healthcare burden of around 3.5 billion US Dollars per year in USA alone. Aims: To explore the prevalence of multiple resistant community-acquired UTI-associated E. coli...

To explore the prevalence of multidrug-resistant community-associated uropathogenic Escherichia coli (UPEC) and their virulence factors in Western Saudi Arabia. A total of 1,000 urine samples were examined for the presence of E. coli by selective plating on MacConkey, CLED, and sheep blood agar. Antimicrobial susceptibility patterns were determined...

Proteus mirabilis is a specific opportunistic pathogen of many infections including urinary tract infections (UTIs). Risk factors are linked with the acquisition of multidrug-resistant (MDR) to 3 or more classes of antimicrobials) strains. The resistance in extended-spectrum alpha-lactamase is rare, but the rising resistance in extended-spectrum be...

Cardamom (Elettaria cardamomum) fruits or capsules are rich in bioactive phytochemicals with beneficial health impacts. Very few studies reported the chemistry and functionality of Elettaria cardamomum lipids, fixed oil, and cold pressed cardamom oil (CPCO). This work characterized CPCO for its lipid classes, fatty acids, phytosterols, tocols, tota...

We investigated the occurrence rate of antibiotic-resistant salmonellae in exotic pet reptiles in Saudi Arabia. Salmonellae samples were collected from eight different genera of pet reptiles (snakes and lizards). Selective enrichment and selective plating procedures were carried out in order to detect salmonellae. Isolated bacteria were identified...

Sobia is a traditional fermented beverage that is well known in the western province of Saudi Arabia particularly during Ramadhan season. The beverage is sold by retailers as well as by anonymous street vendors. We aimed to examine the microbiological qualities of sobia and provide a safety assessment of its consumption. Total counts of aerobic mes...

Background: Urinary tract infections (UTIs) remain a common and leading cause of morbidity worldwide, with more than 150 million people affected annually, resulted in social and healthcare burden of around 3.5 billion US Dollars per year in USA alone. Aims: To explore the prevalence of multiple resistant community-acquired UTI-associated E. coli an...

Background: Urinary tract infections (UTIs) remain a common and leading cause of morbidity worldwide, with more than 150 million people affected annually, resulted in social and healthcare burden of around 3.5 billion US Dollars per year in USA alone. Aims: To explore the prevalence of multiple resistant community-acquired UTI-associated E. coli an...

Biosynthesis of silver nanoparticles (AgNPs) is emerging as a simple and eco-friendly alternative to conventional chemical synthesis methods. The role of AgNPs is expanding as antimicrobial and anticancer agents, sensors, nanoelectronic devices, and imaging contrast agents. In this study, biogenic AgNPs were synthesized using extracts of different...

Biosynthesis of gold nanoparticles (AuNPs) is emerging as a better alternative to traditional chemical-based techniques. During this study, extracts of different marine algae species Ulva rigida (green algae), Cystoseira myrica (brown Algae), and Gracilaria foliifera (red Algae) were utilized as reducing and capping agents to synthesize AuNPs. AuNP...

The fungal biofilm-based infections are resistant to the most of currently available antifungal medications and affect many people worldwide. The pathogenicity of invasive fungal infection is usually accompanied by increased adhesion and the development of complex biofilms. Despite the development of several antifungal drugs that target the fungal...

Background: Urinary tract infections (UTIs) remain a common and leading cause of morbidity worldwide, with more than 150 million people affected annually, resulted in social and healthcare burden of around 3.5 billion US Dollars per year in USA alone. Aims: To explore the prevalence of multiple resistant community-acquired UTI-associated E. coli an...

Purpose Rhizospheric soil fungi are critical for plant and soil health. However, their multiple functional traits and impact on plant growth have not been systematically explored. Methods During this study, biochemical traits of 73 indigenous soil fungal isolates and 15 unidentified isolates related to plant growth promotion and production of extr...

The moderately halophilic strain Salinivibrio sp. EG9S8QL was isolated among 11 halophilic strains from saline mud (Emisal Salt Company, Lake Qarun, Fayoum, Egypt). The lipopolysaccharide was extracted from dried cells of Salinivibrio sp. EG9S8QL by the phenol–water procedure. The OPS was obtained by mild acid hydrolysis of the lipopolysaccharide a...

Imprudent and overuse of clinically relevant antibiotics in agriculture, veterinary, and medical settings contribute to the global epidemic scenario of Antimicrobial Resistance (AMR). There is a growing concern among researchers and stakeholders that the environment has been implicated as an AMR reservoir and plays a key role in the dissemination o...

Bacillus thuringiensis is a Gram-positive bacterium naturally found in soil, water and grain dust. B. thuringiensis has been used for decades as biological control for pests of economical and medical importance. The aim of this study was to test different growth media for the optimal production of vegetative insecticidal protein (Vip3) by B. thurin...

We report in this study for the first time the prevalence of multiple resistant Staphylococcus haemolyticus in clinical settings in Saudi Arabia. A total of 1060 clinical specimens of hospitalized patients were screened for the presence of S. haemolyticus in the period between September and December 2020. Primary identification of the isolates was...

Plastic materials have become a necessity of human life especially in the packaging of food commodities and biomedical procedures. Bioplastic is emerging as an effective alternative to fossil oil-based materials to avoid the environmental hazards of the plastic industry. During this study, chicken feathers were used as a substrate to isolate kerati...

Staphylococcus aureus is a major human pathogen that may cause a wide range of infections and is a frequent cause of soft tissue and bloodstream infections. It is a successful pathogen due to its collective virulence factors and its ability to evade the host immune systems. The review aims to highlight how S. aureus destroys and damage the host cel...

An amendment to this paper has been published and can be accessed via the original article.

The search for effective and bioactive antimicrobial molecules to encounter the medical need for new antibiotics is an encouraging area of research. Plant defensins are small cationic, cysteine-rich peptides with a stabilized tertiary structure by disulfide-bridges and characterized by a wide range of biological functions. The heterologous expressi...

In vitro eradication of the C. albicans and S. mutans mixed biofilms by eugenol alone and in combination with the antimicrobial drugs. Previously characterized strains of C. albicans (CAJ-01 and CAJ-12) and S. mutans MTCC497 were used to evaluate the eradication of biofilms using XTT reduction assay, viability assay, time dependent killing assay an...

In this study fungal profiles of agricultural field soil irrigated with industrial wastewater and sewage containing varying concentrations of heavy metals (Chromium, Nickel, Cobalt, Copper and Cadmium) have been investigated. The impact of long term heavy metal contamination on emergence of heavy metal tolerant soil fungal population, changes in mo...

This chapter reviews the composition and functionality of cold pressed rosemary oil (CPRO). Fatty acids profile, lipid classes, tocols composition, and phenolic content are summarized. Antiradical activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and galvinoxyl free radicals, as well as antimicrobial potential against dermatophytic fungi, and...

Abstract Honeys originating from Sidr (Ziziphus spina‐christi L.) and Talh (Acacia gerrardii Benth.) trees in Saudi Arabia exhibited substantial antimicrobial activity against pathogenic gram‐positive bacteria (Bacillus cereus, Staphylococcus aureus), gram‐negative bacteria (Escherichia coli, Salmonella enteritidis), and a dermatophytic fungus (Tri...

Bird fecal matter is considered a potential source of pathogenic microbes such as yeast species that contaminate the environment. Therefore, it needs to be scrutinized to assess potential environmental health risks. The aim of this study was to investigate the diversity of the yeasts in pigeon fecal droppings, their antifungal susceptibility patter...

Throughout the vegetative life of Bacillus thuringiensis, vegetative insecticidal proteins (Vip) are produced and secreted. In the present study, the vip3 gene isolated from Bacillus thuringiensis, an Egyptian isolate, was successfully amplified (2.4 kbp) and expressed using bacterial expression system. The molecular mass of the expressed protein w...

The emergence and spread of multidrug-resistant (MDR) pathogenic bacteria is a clinical problem that requires novel anti-infective agents. Targeting pathogenic biofilms is considered a promising strategy to control bacterial infections. In this study, bioactive extracts of Carum copticum were investigated for their anti-biofilm efficacy against ext...

Early antibiotic discovery program has witnessed significant role of mainly Streptomyces in antibiotic/drug discovery program. Due to various constraints, both academic and industry levels, the discovery of new antibiotics with novel mode of action is drastically slowed down in the last three decades. Rapid development and spread of multidrug-resis...

Emergence of antibiotic resistance is a never-ending process in the bacteria due its vast capacity to resist and acquire various resistance mechanisms against antibacterial drugs. Campylobacter is a well-known pathogenic bacteria to human and animals and survive in different environment including foods. Species of campylobacters is responsible of g...

Over the last two decades, the development of new antibacterial drugs has been very limited due to many reasons. In light of the alarming situation of antimicrobial resistance (AMR), it is now vital to act promptly to develop new ways to combat the resistance problem through an integrated approach. Despite the slow progress of drug discovery by pha...

Exopolysaccharides (EPS) particularly, from Lactic acid bacteria have received increasing attention in food, medical, and pharmaceutical applications. The present work aims to isolate, characterize and identify exopoly saccharide-producing bacteria from fermented fruits and vegetables and dairy products. A total of 55 isolates were isolated from fe...

Heavy metal contamination due to natural and anthropogenic source is a major environmental problem. Release of metal from use of agrochemicals, industrial effluents and wastewater residues and their accumulation in food causes serious dilemma to animal and human health concern. On the other hand microbial population gets affected from metal toxicit...

Livestock industries are growing tremendously along with the human population. India has rapidly increased its beef exports. The maintenance of quality of food products derived from these animals is on high consideration. In this investigation the prevalence of Salmonella species was performed through phenotypic and genotypic characterization in be...

This study aimed to determine the diversity of antibiotic‐resistant Shiga toxin‐producing Escherichia coli (STEC) serogroups in 600 foodstuff samples of animal origin in the northern part of India. Of the 158 (26.33%) E. coli isolates that were detected in food samples the ‘O’ antigen‐based serotyping revealed the abundance of serogroups O60, O86,...

Campylobacter jejuni is Gram negative spiral-shaped foodborne pathogenic bacterium which belongs to the family Campylobacteriaceae. It is a microaerophillic and thermophillic microorganism that can grow well at the temperature range between 37°C and 42°C. Campylobacter can cause diseases in humans and animals including wild animals, pets and livest...

The aim of the current work was to study the diversity of Staphylococcus spp. in different types of food products that are available to consumers, to assess its safety from a public health standpoint. A total of twenty food samples (raw milk, white cheese, raw minced meat, beef salami and salted fish) were purchased from the markets. Detection of S...

Black cutworm (BCW) is an economically important lepidopteran insect. The control of this insect by a Bt toxin and the understanding of the interaction between the Bt toxin and its receptor molecule were the objectives of this research work. A gene coding for a Vip3A receptor molecule was identified, characterized, and cloned, from the brush border...

Spices and herbs are recognized as sources of natural antioxidants and thus play an important role in the chemoprevention of diseases and aging. Piper cubeba is one among them and known for its medicinal properties for decades. Various biological activities are associated with its extract and phytocompounds. However, the anti-mutagenic activity of...

Black cutworm (BCW) Agrotis ipsilon, an economically important lepidopteran insect, has attracted a great attention. Bacillus thuringiensis (Bt) is spore forming soil bacteria and is an excellent environment-friendly approach for the control of phytophagous and disease-transmitting insects. In fact, bio-pesticide formulations and insect resistant t...

Campylobacteriosis is the common gastrointestinal disease worldwide. However, in many parts of the world, including India, the impact of campylobacteriosis is less commonly investigated. This study aimed to determine the prevalence and antibiotic susceptibility profiles of Campylobacter jejuni in raw poultry meat and poultry-related samples at reta...

The widespread occurrence of extended spectrum β-lactamases (ESβLs) producing enteric bacteria and their co-resistance with flouroquinolones has impaired the current antimicrobial therapy. This has prompted the search for new alternatives through synergistic approaches with herbal extracts. In this study Carum copticum (seeds) was extracted first i...

Quorum sensing is a widespread mechanism in enormous number of bacteria for regulating various gene expression in a cell density-dependent manner through production and recognition of small molecules known as autoinducer. Diverse kinds of quorum-sensing networks are found in different bacterial species. Among various signal molecules, acyl homoseri...

The ecological fitness of soil- and root-associated bacterial communities is a key element for soil fertility and plant health as well as plant stress tolerance. Genetic variability in bacterial populations is maintained through mutation and gene acquisition. Horizontal gene transfer (HGT) is accomplished by conjugation, transformation, and transdu...

The biofilm formation by the microbes is considered as a strategy of survival under natural conditions. Microbes form biofilm on the abiotic surfaces also under in vitro conditions with the availability of optimum nutrients and moisture content. Considerable amount of work on biofilms associated with environment, medical, and food industry has been...

Epidemiological data suggest that Campylobacter remains a worldwide leading cause of gastrointestinal infections. Improperly prepared meat products, unpasteurized milk as well as non chlorinated drinking water were shown to be the main sources of campylobacteriosis. The Campylobacter survival mechanism in various environments facilitated the transm...

Quorum sensing (QS) is a global gene regulatory mechanism in bacteria for various traits including virulence factors. Disabling QS system with anti-infective agent is considered as a potential strategy to prevent bacterial infection. Mangifera indica L. (mango) has been shown to possess various biological activities including anti-QS. This study in...

The present study was aimed to investigate the circulation of four dengue virus (DENV) serotypes in Makkah, Western Saudi Arabia. Blood samples were collected from 25 dengue fever-suspected patients and were subjected to molecular typing for DENV- 1, DENV-2, DENV-3, and DENV-4 serotypes of dengue virus, by reverse transcription polymerase chain rea...

In this study, 120 lactic acid bacterial strains from different fermented dairy products as well as 10 bacterial intestinal isolates were evaluated for in vitro and in vivo degradation of various food azo dyes. Of these isolates, lactic acid bacteria (LAB) strains 13 and 100 and the intestinal isolates Ent2 and Eco5 exhibited 96–98% degradation of...

Some chemical compounds have a significant effect on living organisms. This is shown in the physiological and biological changes that appear on some cells and tissues of the body. The current study aimed to investigate the effect of the dissolved MTBE in drinking water on the mucosal immune cells of the small intestine of the white mice. The presen...