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Husain F. Hassan

Husain F. Hassan

Doctor of Philosophy

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64
Publications
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495
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Publications

Publications (64)
Article
Full-text available
This study is an investigation of the drugs effect on some pathogenic Acanthamoeba isolated from Iraqi waters, where the problem of environmental adaptation that characterizes this organism in addition to being a reservoir for many pathogenic microorganisms that take shelter in it to escape disinfectants and medicines is sometimes difficult to trea...
Article
Full-text available
The growth inhibitory effects of azole derivatives were tested against promastigotes of L. tropica. Ketoconazole, Itraconazole, Nystatin, Clotrimazole and Terbinafine at concentration of 1mM inhibited 70%, 77%, 53%, 39% and 72% respectively, growth of promastigotes whereas Fluconazole at 25mM and Metronidazole at 0.5mg inhibited 78% and 43% respect...
Article
In this study, 25 stray cats were caught from the urban city of Kirkuk hunted using specially designed traps. Cats were dissected and examined in order to investigate its infection with intestinal parasites. Our results indicate that all stray cats were infected with at least one type of helminthes. Three types of Trematodes were identified: Echino...
Article
Full-text available
The aim of this study is to diagnose the parasites, which are present in the Musca domestica insect which are carried on external surface skinappendages and internal surface gastrointestinal tract in which I grouped 150 samples from different places in Kirkuk city. My results of this study revealed that 41samples are infected which mean that the pe...
Article
Amoebas live freely in different climates and parts of the world. Several species of Free Living Amoeba (FLA) are capable of causing serious as well as fatal infections in human beings. The aim of this study was to identify and compare genotypes of water-polluting FLA in major rivers and lakes of Iraq and compare them with FLA isolates from Iran an...
Article
Full-text available
A range of parasites (cultured promastigotes of Leishmania tropica and L. donovani, adult Fasciola gigantica, protoscoleces of Echinococcus granulosus, adult Hymenolepis nana, Moniezia expansa, Joyeuxiella pasqualei and adult Toxocara cati) have been surveyed for the presence of four phosphatase (nitrophenyl phosphatase, naphtho-1-phosphatase, glyc...
Preprint
Full-text available
5'-AMP nucleotidase (EC. 3. 1. 3. 5) from promastigotes of Leishmania tropica and L.donovani was purified approximately 89 fold and 95 fold with specific activity of 30nmol/min/mg protein and 800 nmol/min/mg protein respectively, by column chromatography on CM-cellulose and concanavalin A-Sepharose affinity. The apparent molecular weight was found...
Article
Full-text available
Abstract The present study was established on the slaughtered ruminants in Kirkuk central abattoir between June 1st till December, 31st 2015. The aim of the study is to determine the prevalence of gastro-intestinal parasites (GIPs) of slaughtered grazing ruminants in Kirkuk province. Fresh faecal specimens (n=276) were obtained from slaughtered gra...
Article
Full-text available
ABSTRACT Gastro-intestinal parasites (GIPs) of stray dogs and cats play an epidemiological critical role, and also play a major role in transmitting through contamination of soil, food and/or drinking water with GIPs faecals. The objective of this study was to screening the prevalence of gastrointestinal parasites in stray dogs and cats in Kirkuk p...
Article
Full-text available
In this study a total of 600 suspected cases of cutaneous leishmaniasis at refugee campus in Kirkuk were investigated. Samples were collected during the period of January-March 2015 ,pateints ages were (1-50) years old. Fifty four cases were found to be positive with infection rate 9%. Cutaneous leishmaniasis were more frequent in females (61.11%)...
Article
Full-text available
In this study a total of 600 suspected cases of cutaneous leishmaniasis at refugee campus in Kirkuk were investigated. Samples were collected during the period of January-March 2015 ,pateints ages were (1-50) years old. Fifty four cases were found to be positive with infection rate 9%. Cutaneous leishmaniasis were more frequent in females (61.11%)...
Article
Full-text available
ABSTRACT Objective: Cystic echinococcosis is distributed worldwide and is an important public health challenge in many countries. The present study was an experimental trial to use hydatid antigens derived from viable protoscoleces cultivated at 37 and 45°C for 4 h as a vaccine candidate for protection against hydatid infection. Methods: Balb/c mi...
Article
A B S T RACT The plasma membrane of cells contains enzymes whose active sites face the external medium rather than the cytoplasm. The adenosine tri phosphatase (ATP phosphohydrolase, EC 3.6.1.3.; ATPase) is membrane – bound enzyme which transport protons across the plasma membrane using ATP as an enegy source. In this work, we extracted the ade...
Article
Full-text available
Objective: The adenosine triphosphatase (ATP phosphohydrolase, EC 3.6.1.3.;ATPase) is a membrane -bound enzyme which transport protons across the plasma membrane using ATP as an energy source. Methods: The adenosine triphosphatase (ATPase ; EC: 3.6.1.3) was extracted from membrane preparations of adult Fasciola hepatica by chloroform treatment a...
Article
Full-text available
ABSTRACT Objective: The adenosine triphosphatase (ATP phosphohydrolase, EC 3.6.1.3.;ATPase) is a membrane -bound enzyme which transport protons across the plasma membrane using ATP as an energy source. Methods: The adenosine triphosphatase (ATPase ; EC: 3.6.1.3) was extracted from membrane preparations of adult Fasciola hepatica by chloroform tre...
Article
Trophozoites of Acanthamoeba castellanii were found to incorporate a range of purine bases and nucleosides into parasite nucleic acids. Results from competition studies suggest that A. castellanii is capable of interconverting purine nucleotides. The amoebae contain deaminase, phosphorylase, kinase, phosphoribosyltransferase and 5'-nucleotidase act...
Article
Amastigotes and log-phase promastigotes of Leishmania mexicana mexicana contained distinct acid phosphatase, 3'-nucleotidase and 5'-nucleotidase activities, distinguishable by their response to pH and inhibitors. Both tartrate-sensitive and tartrate-resistant acid phosphatase were present in the two forms, amastigotes possessed less tartrate-resist...
Article
Extracts of Babesia divergens were examined for the enzymes which catalyse purine salvage. Adenosine deaminase (EC 3.5.4.4), guanine deaminase (EC 3.5.4.3), inosine phosphorylase (EC 2.4.2.1), purine phosphoribosyltransferases (EC 2.4.2.7, EC 2.4.2.8, EC 2.4.2.22) and nucleoside kinases (EC 2.7.1.15, EC 2.7.1.20, EC 2.7.1.73) were all detected at r...
Chapter
Our knowledge of the chemistry of the surface of leishmanias has increased considerably over recent years and notable amongst the advances have been the discoveries of the surface location of three phosphomonoesterase activities (3′-nucleotidase, 5′-nucleotidase and acid phosphatase) (Dwyer & Gottlieb 1984, Gottlieb & Dwyer 1981) and a proteinase (...
Article
The enzymes that catalyse the salvage of purines in Entamoeba histolytica trophozoites have been surveyed. Adenine deaminase (EC 3.5.4.2), adenosine deaminase (EC 3.5.4.4), guanine deaminase (EC 3.5.4.3), adenine phosphoribosyltransferase (PRTase) (EC 2.4.2.7), xanthine PRTase (EC 2.4.2.22) and hypoxanthine PRTase (EC 2.4.2.8) were all detected in...
Article
A range of trypanosomatids (amastigotes and cultured promastigotes of Leishmania mexicana mexicana, cultured promastigotes of L. m. amazonensis, L. donovani and L. tarentolae, culture forms of Crithidia fasciculata, Herpetomonas muscarum muscarum and H. m. ingenoplastis and procyclic trypomastigotes of Trypanosoma brucei brucei) have been surveyed...
Article
Cultured promastigote and isolated amastigote forms of Leishmania mexicana mexicana have been surveyed for the presence of enzymes involved in purine metabolism. Quantitative but not qualitative differences between the enzymes of two forms were discovered. There were found to be significant differences between the enzyme content of L. m. mexicana a...
Article
Leishmania mexicana mexicana cultured promastigotes were fractionated by isopycnic centrifugation on linear sucrose gradients. Guanine, hypoxanthine and xanthine phosphoribosyltransferase activities were found to be associated with glycosomes, whereas adenine phosphoribosyltransferase was cytosolic. 3'- and 5'-nucleotidases and IMP dehydrogenase we...
Article
Amastigotes and cultured promastigotes of Leishmania mexicana mexicana and L. m. amazonensis, cultured promastigotes of L. donovani and L. tarentolae, and the culture forms of Crithidia fasciculata, Herpetomonas muscarum muscarum and H. m. ingenoplastis all possessed four phosphoribosyltransferase (PRTase) activities: adenine PRTase, hypoxanthine P...

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