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Hugo Galvão Pereira

Hugo Galvão Pereira
GreenCoLab

PhD

About

111
Publications
33,615
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Introduction
Hugo Galvão Pereira is currently the innovation manager of GreenCoLab. Previously, he was a research collaborator in the MarBiotech group (CCMAR) and in the R&D departments of Necton (Portugal) and AllMicroalgae (Portugal). He completed his PhD at the University of Algarve, which focused on the production of biomass at industrial scale and the establishment of a microalgae-based biorefinery for different biotechnological applications, in collaboration with LNEG (Portugal) and Sparos (Portugal).

Publications

Publications (111)
Article
Full-text available
Bioprospecting for novel microalgal strains is key to improving the feasibility of microalgae-derived biodiesel production. Tetraselmis sp. CTP4 (Chlorophyta, Chlorodendrophyceae) was isolated using fluorescence activated cell sorting (FACS) in order to screen novel lipid-rich microalgae. CTP4 is a robust, euryhaline strain able to grow in seawater...
Article
Full-text available
Carotenoids are a wide group of lipophylic isoprenoids synthesized by all photosynthetic organisms and also by some non-photosynthetic bacteria and fungi. Animals, which cannot synthesize carotenoids de novo, must include them in their diet to fulfil essential provitamin, antioxidant, or colouring requirements. Carotenoids are indispensable in ligh...
Article
Light-emitting diodes (LEDs) will become one of the world’s most important light sources and their integration in microalgal production systems (photobioreactors) needs to be considered. LEDs can improve the quality and quantity of microalgal biomass when applied during specific growth phases. However, microalgae need a balanced mix of wavelengths...
Article
Full-text available
As mammals are unable to synthesize essential polyunsaturated fatty acids (PUFA), these compounds need to be taken in through diet. Nowadays, obtaining essential PUFA in diet is becoming increasingly difficult; therefore this work investigated the suitability of using macroalgae as novel dietary sources of PUFA. Hence, 17 macroalgal species from th...
Article
Full-text available
We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during...
Poster
Full-text available
Drying Microalgae Using an Industrial Solar Dryer: A Biomass Quality Assessment
Article
Full-text available
Microalgae have become a promising novel and sustainable feedstock for meeting the rising demand for food and feed. However, microalgae-based products are currently hindered by high production costs. One major reason for this is that commonly cultivated wildtype strains do not possess the robustness and productivity required for successful industri...
Article
Full-text available
Microalgae are considered a promising resource of proteins, lipids, carbohydrates, and other functional biomolecules for food and feed markets. Competitive drying solutions are required to meet future demands for high-quality algal biomass while ensuring proper preservation at reduced costs. Since often used drying methods, such as freeze or spray...
Article
Full-text available
Algae have been consumed for millennia in several parts of the world as food, food supplements, and additives, due to their unique organoleptic properties and nutritional and health benefits. Algae are sustainable sources of proteins, minerals, and fiber, with well-balanced essential amino acids, pigments, and fatty acids, among other relevant meta...
Article
Microalgae are emerging as sustainable sources of a wide range of high-value compounds. However, the knowledge about microalgae polar lipids is still limited, despite their interest due to their chemical diversity and bioactivity. This study shows, for the first time, the polar lipidome of the haptophyte microalga Chrysotila pseudoroscoffensis unve...
Article
Full-text available
Growth in most microalgal mass cultivation systems is light-limited, particularly in raceway ponds (RWP) where the light path is higher. Artificial lighting can be a promising solution to diminishing dark zones and enhance microalgal productivity. Therefore, our goal was to prevent the cell shift from photosynthesis to a respiration-only stage by r...
Article
Nannochloropsis is a genus of eicosapentaenoic acid-rich microalgae with high levels of value-added polar lipids. However, the polar lipid composition of microalgal biomass is highly dependent on culture conditions (e.g., light or temperature), which are significantly different under indoor and outdoor culture conditions. In this study, we sought t...
Article
Full-text available
The objective of this techno-economic analysis is to define the costs for an industrial microalgae production process, comparing different operation strategies (Nannochloropsis oceanica cultivation during the whole year or cultivation of two species, where Phaeodactylum tricornutum and Tisochrysis lutea alternate), production scales (1 and 10 ha),...
Article
Full-text available
Microalgae are considered a promising source of eco-friendly antifungal agents that can potentially reduce the usage of synthetic fungicides. Hence, properties of aqueous extracts from Nannochloropsis sp., Phaeodactylum tricornutum, Scenedesmus obliquus, Chlorella vulgaris and Spirulina sp. were investigated in vitro regarding their antagonistic ac...
Article
Full-text available
S. costatum and C. calcitrans are two cosmopolitan high-value centric diatoms, with a rich nutritional profile. The following work optimised the culture medium of S. costatum and C. calcitrans cultures, respectively, in a stepwise process as follows: 2.4 mM and 1.2 mM of silicate, 4 mM of nitrate, 100 µM of phosphate, 20 and 80 µM iron, and 0.5 mL...
Preprint
Fucoxanthin is a xanthophyll carotenoid with high market value. Currently, seaweeds are the primary feedstock for fucoxanthin industrial production. However, marine microalgae reach 5 to 10 times higher concentrations (2.24 to 26.6 mg g-1 DW), and are considered a promising source. In this work, two marine microalgae were produced at industrial sca...
Article
Bioprospecting programmes are the key to increasing the current portfolio of indigenous microalgal strains accessible for different applications in microalgal biotechnology. In this work, nine fastgrowing microalgal strains isolated from the Arabian/Persian Gulf coastal waters of Kuwait were evaluated for their potential as biofuel feedstocks. 18S...
Article
Diatoms are currently considered valuable feedstocks for different biotechnological applications. To deepen the knowledge on the production of these microalgae, the diel pattern of batch growth, photosystem II performance, and accumulation of target metabolites of two commercially relevant diatoms,Phaeodactylum tricornutum and Skeletonema costatum,...
Article
A recently isolated microalgal strain of Skeletonema sp. (Skt) has shown relevant bioactivities. Novel functional foods incorporating this strain were studied: a 3%, w/w, Skeletonema cookie (Skt Ck) and a 2%, w/w, Skeletonema sauce (Skt Sc), which were compared with a control cookie (Ctl Ck) and a control sauce (Ctl Sc), respectively. The fatty aci...
Conference Paper
Animal welfare is defined by a complexity of factors, where good rearing practices promote physical health, low stress, lack of mental suffering, and the opportunity for positive experiences. Different strategies are used to improve animal welfare, such as environmental enrichment (Stevens et al., 2021). This strategy has mainly been applied us...
Article
In-situ chlorophyll a fluorescence measurements were applied to monitor changes in the photochemical variables of Nannochloropsis oceanica cultures under nitrogen-deplete and nitrogen-replete (control) conditions. In addition, growth, lipid, fatty acid, and pigment contents were also followed. In the control culture, growth was promoted along with...
Article
Full-text available
Fucoxanthin is a xanthophyll carotenoid with high market value. Currently, seaweeds are the primary source for fucoxanthin industrial production. However, marine microalgae reach 5 to 10 times higher concentrations (2.24 to 26.6 mg g⁻¹ DW) and are considered a promising feedstock. In this work, two marine microalgae were produced at industrial scal...
Article
In this study, two carotenoid-rich strains of the euryhaline microalga Tetraselmis striata CTP4 were isolated by random mutagenesis combined with selection via fluorescence activated cell sorting and growth on norflurazon. Both strains, ED5 and B11, showed an up to 1.5-fold increase in carotenoid contents as compared with the wildtype, independent...
Preprint
In this work, we applied online chlorophyll a fluorescence measurements to monitor the changes in the photochemical parameters both in nitrate-deplete and nitrate-replete cultures of Nannochloropsis oceanica, in addition to biochemical parameters such as growth, lipid, fatty acid, and pigment contents. Under nitrate-replete conditions, growth was p...
Article
Full-text available
Microalgae are currently considered to be a promising feedstock for biodiesel production. However, significant research efforts are crucial to improve the current biomass and lipid productivities under real outdoor production conditions. In this context, batch, continuous and semi-continuous operation regimes were compared during the Spring/Summer...
Chapter
Polyunsaturated fatty acids (PUFAs) are essential compounds for human health, being responsible for a series of biological processes including reduction of inflammation and prevention of neurological and cardiovascular diseases. Because they are not naturally produced by vertebrates, they need to be present in the diet. Microalgae are rich in PUFAs...
Article
Full-text available
Tisochrysis lutea is a marine haptophyte rich in omega-3 polyunsaturated fatty acids (e.g., docosahexaenoic acid (DHA)) and carotenoids (e.g., fucoxanthin). Because of the nutraceutical applications of these compounds, this microalga is being used in aquaculture to feed oyster and shrimp larvae. In our earlier report, T. lutea organic crude extract...
Article
Full-text available
As the periodic emission of light pulses by light emitting diodes (LEDs) is known to stimulate growth or induce high value biocompounds in microalgae, this flashing light regime was tested on growth and biochemical composition of the microalgae Nannochloropsis gaditana, Koliella antarctica and Tetraselmis chui. At low flashing light frequencies (e....
Presentation
Metabolic bone disorders such as osteoporosis affect millions of people worldwide and drugs currently available have a limited efficacy and have associated secondary effects. There is a compelling need for new compounds with bone anabolic properties acting through new and original mechanisms of action. In this regard, recent works have identified m...
Article
Full-text available
Polar lipids from microalgae have aroused greater interest as a natural source of omega-3 (n-3) polyunsaturated fatty acids (PUFA), an alternative to fish, but also as bioactive compounds with multiple applications. The present study aims to characterize the polar lipid profile of cultured microalga Emiliania huxleyi using hydrophilic interaction l...
Article
Full-text available
Microalgae are widely used as food supplements due to their high protein content, essential fatty acids and amino acids as well as carotenoids. The addition of microalgal biomass to food products (e.g., baked confectioneries) is a common strategy to attract novel consumers. However, organoleptic factors such as color, taste and smell can be decisiv...
Article
The forecasted growth of the aquaculture sector requires the use of novel and sustainable ingredients in aquaculture feeds. A study was undertaken to evaluate the effect of a 10% incorporation of defatted microalgal biomass (DMB) of Tetraselmis sp. CTP4, used at the expense of dehulled solvent-extracted soybean meal (SBM), on the growth performance...
Article
Full-text available
Bioprospection of novel autochthonous strains is key to the successful industrial-scale production of microalgal biomass. A novel Chlorococcum strain was recently isolated from a pond inside the industrial production facility of Allmicroalgae (Leiria, Portugal). Phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences sug...
Article
Full-text available
Thraustochytrids have gained increasing relevance over the last decades, due to their fast growth and outstanding capacity to accumulate polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA). In this context, the present work aimed to optimize the growth performance and DHA yields by improving the culture medium of Aurantioch...
Article
Full-text available
Recently isolated microalgae Tetraselmis sp. IMP3, Tetraselmis sp. CTP4, and Skeletonema sp. were studied. The three novel strains contained relatively high levels of polyunsaturated fatty acids (PUFA) and n3 PUFA. However, highly unsaturated n3 FA contents were relatively low (5.7–13.0% of the total FA). In general, eicosapentaenoic acid (EPA, 20:...
Article
Full-text available
Microalgal biomass has gained increasing attention in the last decade for various biotechnological applications, including human nutrition. Organic products are currently a growing niche market in which the food industry has shown great interest. In this context, this work aimed at developing an organic culture medium for the production of autotrop...
Article
Full-text available
Raceways ponds are the microalgal production systems most commonly used at industrial scale. In this work, two different raceway configurations were tested under the same processing conditions to compare their performance on the production of Nannochloropsis oceanica. Biomass productivity, biochemical composition of the produced biomass, and power...
Article
Holothuria arguinensis aquaculture started to be developed in 2014, being the first sea cucumber species from Europe. However, some aspects of its aquaculture biotechnology need to be assessed. This work aimed to evaluate seagrass debris of Zostera noltii and Cymodocea nodosa as food source for broodstock maintenance in tanks, during the breeding p...
Article
The industrial microalga Tetraselmis sp. CTP4 is a promising candidate for aquaculture feed, novel food, cosmeceutical and nutraceutical due to its balanced biochemical profile. To further upgrade its biomass value, carotenogenesis was investigated by testing four environmental factors, namely temperature, light intensity, salinity and nutrient ava...
Article
Full-text available
Industrial scale-up of microalgal cultures is often a protracted step prone to culture collapse and the occurrence of unwanted contaminants. To solve this problem, a two-stage scale-up process was developed – heterotrophically Chlorella vulgaris cells grown in fermenters (1st stage) were used to directly inoculate an outdoor industrial autotrophic...
Article
Full-text available
Commercial production of microalgal biomass for food and feed is a recent worldwide trend. Although it is common to publish nutritional data for microalgae grown at the lab-scale, data about industrial strains cultivated in an industrial setting are scarce in the literature. Thus, here we present the nutritional composition and a microbiological an...
Article
Full-text available
The photosynthetic efficiency (PE) and potential of Phaeodactylum tricornutum for CO2 mitigation in industrial tubular photobioreactors (PBRs) of different volumes were evaluated. A preliminary assay was performed at lab-scale to optimize the salt concentration of the culture medium. Interestingly, salinity did not affect the growth of P. tricornut...
Article
Full-text available
Biomass harvesting is one of the most expensive steps of the whole microalgal production pipeline. Therefore, the present work aimed to understand the effect of salinity on the growth performance, biochemical composition and sedimentation velocity of Tetraselmis sp. CTP4, in order to establish an effective low-cost pilot-scale harvesting system for...
Article
Full-text available
Cystoseira is a common brown algal genus widely distributed throughout the Atlantic and Mediterranean regions whose taxonomical assignment of specimens is often hampered by intra- and interspecific morphological variability. In this study, three mitochondrial regions, namely cytochrome oxidase subunit 1 (COI), 23S rDNA (23S), and 23S-tRNAVal interg...
Data
Information on the sequences included in this study—species, geographical origin, voucher, GenBank accession numbers and haplotypes. (PDF)
Data
Evolutionary divergence between mt-spacer Cystoseira sequences. (PDF)
Data
Morphological traits identified by other authors for the different Cystoseira phylogenetic groups included in this study. (PDF)
Data
Maximum likelihood phylogenetic tree obtained with RAxML and based on the COI sequences of the samples from Cystoseira genus. Values on the branches represent maximum likelihood bootstrap support values ≥ 75 on the left, and Bayesian posterior probabilities ≥ 90% on the right. Information of the sequences included in this tree are indicated in S1 T...
Data
Bayesian phylogenetic tree obtained with MrBayes and based on the COI sequences of the samples from Cystoseira genus. Values on the branches represent Bayesian posterior probabilities ≥ 90%. Information of the sequences included in this tree are indicated in S1 Table. (PNG)
Data
Median-Joining networks of Cystoseira-III mt-spacer, 23S and COI haplotypes. Pie charts are proportional to haplotype frequencies. Theoretical median vectors are represented by black dots. Colors represent the different Cystoseira species as described in the legend. (PNG)
Data
Evolutionary divergence between COI Cystoseira sequences. (PDF)
Data
Bayesian phylogenetic tree obtained with MrBayes and based on concatenated COI-23S-mt-spacer sequences of the samples from Cystoseira genus. Values on the branches represent Bayesian posterior probabilities ≥ 90%. Information of the sequences included in this tree are indicated in S1 Table. (PNG)
Data
Median-Joining networks of Cystoseira-I mt-spacer, 23S and COI haplotypes. Pie charts are proportional to haplotype frequencies. Theoretical median vectors are represented by black dots. Colors represent the different Cystoseira species as described in the legend. (PNG)
Data
Bayesian phylogenetic tree obtained with MrBayes and based on concatenated COI 23S-mt-spacer sequences of the samples from the Sargassaceae family. Values on the branches represent Bayesian posterior probabilities ≥ 90%. Information of the sequences included in this tree are indicated in S1 Table. (PNG)
Data
Evolutionary divergence between 23S Cystoseira sequences. (PDF)
Data
Maximum likelihood phylogenetic tree obtained with RAxML and based on the 23S sequences of the samples from the Cystoseira genus. Values on the branches represent maximum likelihood bootstrap support values ≥ 75 on the left, and Bayesian posterior probabilities ≥ 90% on the right. Information of the sequences included in this tree are indicated in...
Data
Bayesian phylogenetic tree obtained with MrBayes and based on the 23S sequences of the samples from Cystoseira genus. Values on the branches represet Bayesian posterior probabilities ≥ 90%. Information of the sequences included in this tree are indicated in S1 Table. (PNG)
Data
Maximum likelihood phylogenetic tree obtained with RAxML and based on the mt-spacer sequences of the samples from Cystoseira genus. Values on the branches represent maximum likelihood bootstrap support values ≥ 75 on the left, and Bayesian posterior probabilities ≥ 90% on the right. Information of the sequences included in this tree are indicated i...
Data
Bayesian phylogenetic tree obtained with MrBayes and based on the mt-spacer sequences of the samples from Cystoseira genus. Values on the branches represent Bayesian posterior probabilities ≥ 90%. Information of the sequences included in this tree are indicated in S1 Table. (PNG)
Data
Median-Joining networks of Cystoseira-II mt-spacer, 23S and COI haplotypes. Pie charts are proportional to haplotype frequencies. Theoretical median vectors are represented by black dots. Colors represent the different Cystoseira species as described in the legend. (PNG)
Chapter
Microalgae accumulate a variety of compounds that form the basis for potential marketable products as whole cell biomass or as fractions after an extraction process. A cascading biorefinery approach aims at recovering and separating multiple algae compounds to valorise the complete biomass and to improve the economics of the value chain. This chapt...