Hugo A. Guillen-Ramirez

Hugo A. Guillen-Ramirez
University College Dublin | UCD · School of Biology and Environmental Science

PhD in Computer Science

About

16
Publications
1,598
Reads
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86
Citations
Citations since 2017
13 Research Items
84 Citations
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2017201820192020202120222023051015202530
2017201820192020202120222023051015202530

Publications

Publications (16)
Article
Full-text available
CRISPR-Cas9 deletion (CRISPR-del) is the leading approach for eliminating DNA from mammalian cells and underpins a variety of genome-editing applications. Target DNA, defined by a pair of double-strand breaks (DSBs), is removed during nonhomologous end-joining (NHEJ). However, the low efficiency of CRISPR-del results in laborious experiments and fa...
Article
Full-text available
Abstract The increasing interest in bioactive peptides with therapeutic potentials has been reflected in a large variety of biological databases published over the last years. However, the knowledge discovery process from these heterogeneous data sources is a nontrivial task, becoming the essence of our research endeavor. Therefore, we devise a uni...
Article
Riboswitches are non-coding RNAs that regulate gene expression by altering the structural conformation of mRNA transcripts. Their regulation mechanism might be exploited for interesting biomedical applications such as drug targets and biosensors. A major challenge consists in accurately identifying metabolite-binding RNA switches which are structur...
Article
We describe a method for inverting spectroscopic data of the absorption and extinction properties of colloidal samples of resonant particles. We show that, with some prior knowledge, the genetic algorithm employed is able to estimate the probability density function of particle sizes. Since the data are sensitive to the shape and material of the pa...
Preprint
Full-text available
Long noncoding RNAs (lncRNAs) can positively and negatively regulate expression of target genes encoded in cis. However, the extent, characteristics and mechanisms of such cis-regulatory lncRNAs (cis-lncRNAs) remain obscure. Until now, they have been defined using inconsistent, ad hoc criteria that can result in false-positive predictions. Here, we...
Article
Full-text available
Long noncoding RNAs (lncRNAs) are widely dysregulated in cancer, yet their functional roles in cancer hallmarks remain unclear. We employ pooled CRISPR deletion to perturb 831 lncRNAs detected in KRAS-mutant non-small cell lung cancer (NSCLC) and measure their contribution to proliferation, chemoresistance, and migration across two cell backgrounds...
Preprint
Full-text available
Long noncoding RNAs (lncRNAs) can act as tumour suppressor or oncogenes to contrast/promote tumour cell proliferation via RNA-dependent mechanisms. Recently, genome sequencing has identified elevated densities of tumour somatic single nucleotide variants (SNVs) in lncRNA genes. However, this has been attributed to phenotypically-neutral “passenger”...
Preprint
Full-text available
Tumour DNA contains thousands of single nucleotide variants (SNVs) in non-protein-coding regions, yet it remains unclear which are driver mutations that promote cell fitness. Amongst the most highly mutated non-coding elements are long noncoding RNAs (lncRNAs), which can promote cancer and may be targeted therapeutically. We here searched for evide...
Preprint
Full-text available
Long noncoding RNAs (lncRNAs) are widely dysregulated in cancer, yet their functional roles in cellular disease hallmarks remain unclear. Here we employ pooled CRISPR deletion to perturb all 831 lncRNAs in KRAS-mutant non-small cell lung cancer (NSCLC), and measure their contribution to proliferation, chemoresistance and migration across two cell b...
Article
Full-text available
MOTIVATION: Bioactive peptides have gained great attention in the academy and pharmaceutical industry since they play an important role in human health. However, the increasing number of bioactive peptide databases is causing the problem of data redundancy and duplicated efforts. Even worse is the fact that the available data is non-standardized an...
Conference Paper
Los algoritmos de aprendizaje automático supervisado están limitados tanto por el etiquetado de las instancias a aprender, como del conocimiento del dominio de los problemas a resolver. Extraer dicho conocimiento sin intervención humana es una tarea compleja que pudiera realizarse utilizando algoritmos de aprendizaje por refuerzo, entre los que se...
Code
Full-text available
This is a brief introduction to the refractivesqlite package (https://github.com/HugoGuillen/refractiveindex.info-sqlite) developed by Hugo Guillén (https://github.com/HugoGuillen). This package is a wrapper in Python 3 for the database of optical constants refractiveindex.info (http://refractiveindex.info/) developed by Mikhail Polyanskiy (https:/...
Presentation
Full-text available
Se muestran algunos recursos en línea para ayudar a aumentar la detectabilidad (discoverability) del trabajo de investigación.
Conference Paper
The development of new technologies for the design of DNA microarrays has boosted the generation of large volumes of biological data, which requires the development of efficient computational methods for their analysis and annotation. Among these methods, biclusters generation algorithms attempt to identify coherent associations of genes and experi...

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