Huber Johannes

Isoflavones have emerged as one of the plant components studied most thoroughly by medical researchers in recent decades; in the scientific discussion, the wide variety of their effects draws attention to ever-new aspects that explain the protective effect of this group of substances that have been part of the human diet for millennia. The most rec...

The link between higher uptake of isoflavones and a reduced frequency of menopause-related hot flushes were first described in 1992 based on a lower incidence of hot flushes in countries with high dietary soy intake1. Since then, a number of clinical trials with different sources of isoflavones, including soy and red clover, have been performed, an...

The association between an increased uptake of isoflavones and a reduced frequency of menopausal hot flushes was first described in 1992, based on a lower incidence of hot flushes in countries with a high consumption of soy. Since then, numerous clinical trials with various sources of isoflavones including soy and red clover have been presented, wi...

Dendritic cells (DCs) are antigen-presenting cells, which are mediated by MHC-class II molecules reacting with T-helper cells, eliciting a broad spectrum of immune reactions at cellular and humoral levels depending on their subtypes. DCs are also able to cross-present peptides from intracellular proteins as well as from intracellular pathogens via...

This guideline has been developed based on studied and clinical investigations. Therefore, it appears to be appropriate to use all the available evidence, which are very encouraging, in a summarized form to propose guidelines by a group of European experts in order to give the gynecologists, obstetricians and reproductive medicine specialists have...

Sleep disturbance is a prevalent health problem that increases with age and occurs more frequently in women than in men. In women, insomnia increases particularly as women approach and pass through menopause. Insomnia in older women is one of the most common symptoms of menopause and it can either be a part of the climacteric syndrome or can occur...

Age Typical Associations between Skeletal Muscle Mass and Bone Mass among Healthy Women Human body composition changes through the process of ageing. Beside a well documented increase in fat mass, bone mass and muscle mass decline progressively, resulting in pathologically deceased skeletal muscle mass, i.e. sarcopenia and osteoporosis, in the wors...

This paper examines sex specific associations between soft tissue body composition (lean and fat mass) and bone mineral density among older adults. Two hundred and eighty-two subjects (152 females and 130 males) aged 60-92 years ((x))= 71.9 ± 7.9) were enrolled. Body composition of the whole body and bone density (BMD) of the whole body and the fem...

To evaluate whether parameters for calcium metabolism were associated with characteristics of polycystic ovary syndrome (PCOS). A prospective cohort study. Ninety-one anovulatory, infertile women with PCOS patients underwent clomiphene citrate (CC) stimulation. Main outcome measures were parathyroid hormone (PTH); 25-hydroxyvitamin D3 (25OHD3); ser...

The aim of this study was to investigate the potential effects of pomegranate seed oil (PGS) on menopausal symptoms. The prospective randomized, placebo-controlled, double-blinded trial was completed by 81 postmenopausal women, who received two daily doses of either 30 mg PGS containing 127 μg of steroidal phytoestrogens per dose or a placebo for 1...

Der Rheumabegriff der Gegenwart stellt ein sehr komplexes und heterogenes Gebilde dar, Sinn und Gehalt des Begriffes „Rheuma“ können nur aus seiner geschichtlichen Entwicklung und Wandlung heraus verstanden werden.

To compare laparoscopic-assisted vaginal hysterectomy (LAVH) with and without bipolar vessel sealing (BVS) using the LigaSure™ device by meanings of intra- and postoperative course. In a retrospective case-control study 362 female patients (mean age 54.3 ± 9.5 years) were included who underwent LAVH with (n = 252) and without (n = 110) use of BVS....

To establish the prevalence of endometriosis metastatic to pelvic sentinel lymph nodes (PSLN) in women with ovarian and/or peritoneal endometriosis. Prospective clinical study. Academic research institution. Women with a laparoscopic diagnosis of ovarian and/or peritoneal endometriosis verified by intraoperative frozen section analysis. Resection o...

This study investigated the satisfaction of women carrying the LNG-IUD and determined influencing factors, especially considering bleeding patterns and body mass index (BMI). Cross sectional study. Gynecological offices in a Central European district. 1825 women aged between 18 and 60 years. While sitting in the waiting room, voluntary patients had...

Recent studies showed that hormones like progesterone, testosterone, etc. can eject [Formula: see text] (solvated electrons). By means of electron transfer processes via the brain, the hormones communicate with other biological systems in the organism. The present study proves that also estrone is able to emit electrons. Their yield strongly depend...

To establish an association between the number of oocytes retrieved after controlled ovarian hyperstimulation (COH) and saliva cortisol (F) levels, as well as subjective stress, in women undergoing their first IVF cycle. Prospective clinical study. Academic research institution. Women with primary or secondary infertility undergoing IVF. Fertility...

Cross-sex hormone therapy (CSHT) is known to lead to alterations in the serum lipid profile. However, the available reports in the literature are problematic, because of methodological limitations. To assess changes in the fasting serum lipid profile during CSHT, including long-term follow-up. Retrospective chart analysis of all 89 male-to-female (...

Hashimoto's thyroiditis (HT) is a common disease, and is the most prevalent cause of hypothyroidism. Symptoms and diseases associated with HT are considered to be caused by hypothyroidism. We hypothesized that higher antithyroperoxidase (anti-TPO) antibody levels would be associated with an increased symptom load and a decreased quality of life in...

The aim of our study was to review our experience with a modified technique of laparoscopic ovarian drilling (LOD) using a monopolar hook electrode. We retrospectively included 38 clomiphene-resistant women with polycystic ovary syndrome undergoing LOD. A laparoscopic monopolar hook electrode was used to make three to six incisions in the ovarian c...

Low-grade endometrial stromal sacomas (ESS) are estrogen-sensitive tumors. Polymorphic variation in the CYP19 gene can affect estrogen synthesis by increasing aromatase activity resulting in elevated levels of estrone and estradiol. We examined the polymorphism 1558 C > T in he aromatase gene (CYP19A1) in a series of 20 low-grade endometrial stroma...

To evaluate gene expression signatures of breast tissue in female-to-male (FtM) transsexuals under cross-sex hormone therapy (HT). Prospective cohort study. Academic research institution. Five hormone-naïve FtM transsexuals before and after HT. Breast tissue biopsy before and after 2 years of intramuscular testosterone undecanoate (1,000 mg every 1...

In infertile women suffering from polycystic ovary syndrome (PCOS), anti-thyroid peroxidase antibodies values exceeding the upper level of normal were found in significantly more clomiphene citrate resistant patients compared clomiphene citrate responders and metformin responders. Thus, elevated antiTPO levels are associated with poor treatment res...

In women with a retroverted uterus, who have dyspareunia, chronic pelvic pain, or dysmenorrhea, laparoscopic ventrosuspension of the uterus has been reported effective in achieving symptom relief. To critically review our experience with our method of laparoscopic ventrosuspension. Cohort study (Canadian Task Force classification II-3). Tertiary ca...

To critically review our experience with the method of laparoscopic ovarian tissue harvesting for ovarian cryopreservation. In a retrospective cohort study 85 patients undergoing surgery for cryopreservation of ovarian tissue were included. One random ovarian cortical slice was histologically examined in order to determine the presence of primordia...

Based on previous investigations on several hormones, 17α-hydroxyprogesterone (17α-HOPRG) was studied in respect to cancer initiation by its metabolites resulting from electron emission. The emission of electrons (e⁻(aq)) from its singlet excited state of 17α-HOPRG and HPLC-analysis of products were studied. Possible carcinogenicity of metabolites...

Background: Based on the recent findings about the ability of sexual hormones to emit electrons (e aq – ) and to act as electron mediator, it was of interest to investigate adrenaline as an important neurotransmitter. Materials and methods: Highest purity adrenaline (ADR) and chemicals were used for preparation of aqueous solutions (pH ∼7.4). The e...

Initial publications examining the hereditary aspects of endometriosis appeared in the early seventies and demonstrated an up to seven-fold risk for endometriosis in first-degree relatives of endometriosis patients. The aim was to evaluate the influence of hereditary aspects on the endometriosis risk in our patient collective. In a retrospective co...

Based on the different behaviour of 17beta-estradiol (17betaE(2)) and progesterone (PRG), it was of interest to investigate the interaction of both hormones in respect of their electron emission and cytotoxicity by experiments in vitro. The studies include determination of emitted electrons (e(-)(aq)) by the individual hormones as well as by their...

Septate uterus, one of the most common forms of congenital uterine malformations, negatively affects female reproductive health. In a retrospective cohort study, we evaluated the reproductive outcome after hysteroscopic septoplasty in 64 women with septate uterus and primary or secondary infertility. We performed a systematic review of studies eval...

Ovarian stimulation in women with polycystic ovary syndrome (PCOS) increases the risk for perinatal complications. Ovulation induction by laparoscopic ovarian drilling (LOD) might improve the overall pregnancy outcomes. The aim of our study was to assess the adverse events or effects on pregnancy of LOD and clomiphene citrate (CC) stimulation in pa...

Fragestellung: Weibliche transsexuelle Personen werden mit hochdosierter gegengeschlechtlicher Hormontherapie behandelt. Die Auswirkungen dieser Therapie auf das Genexpressionsmuster des Brustgewebes sind unbekannt. Methodik: Im Rahmen einer prospektiven Kohortenstudie verglichen wir das Genexpressionsprofil von mittels Bruststanzen gewonnenem Brus...

Preliminary studies have shown that systemic beta-human chorionic gonadotrophin (betaHCG) therapy alleviates endometriosis-related chronic pelvic pain. The underlying mechanism, however, is completely unknown. This study has investigated the dose-dependent alterations in the overall gene expression profile of endometriosis-derived stromal cells und...

The stem cell marker Octamer-4 (OCT-4) is expressed in human endometrium. Menstrual cycle-dependency of OCT-4 expression has not been investigated to date. In a prospective, single center cohort study of 98 women undergoing hysteroscopy during the follicular (n = 49) and the luteal (n = 40) phases of the menstrual cycle, we obtained endometrial sam...

Supplemental Figure 1: OCT_4_Western_Blots

To present the case of a postmenopausal woman, who was suspected of having an ovarian cyst. Instead, a cystadenoma of the appendix was discovered during laparoscopy. A 64-year-old postmenopausal nulliparous woman was admitted to our hospital because of a cystic lesion, which had been detected in the course of a routine gynecological examination. Th...

Sex reassignment surgery is an important step for transsexuals, since it is known to help the patients to live more easily in their gender role and to significantly increase quality of life. To critically evaluate our experience with the combined procedure of hysterectomy, bilateral salpingo-oophorectomy, and bilateral mastectomy for female-to-male...

The present work reports on the effect of oxidizing (OH, O(2)(*-)) and reducing free radicals (e(-)(aq), H) on 17beta-estradiol (17betaE2) in respect to breast cancer initiation. The objectives of the study were based on the following premise: the ability of 17betaE2 to emit electrons (e(-)(aq)) as well as to transfer them to other biological syste...

A unique feature of steroids is their capacity for metamorphosis. Steroid hormones can be transformed into various metabolites exerting diverse effects. Converted steroids can be inactive, result in excretion of the steroid, occupy different receptors, and sometimes even show antagonistic effects to the precursor. This phenomenon is well documented...

Background: The process of ageing is accompanied with a progressive decline in skeletal muscle mass result-ing in sarcopenia. Aims: To test the association patterns between body mass index and appendicular skeletal muscle mass adjusted for height and total skeletal muscle mass adjusted for height among healthy women. Design: In a cross-sectional st...

Our objective was to investigate luteinizing hormone, follicle-stimulating hormone, testosterone, and androstenedione as predicitve markers for ovulation after laparoscopic ovarian drilling. We retrospectively analyzed 100 clompihen-resistant patients with the polycystic ovary syndrome who underwent laparoscopic ovarian drilling at our department....

Umbilical cord blood (UCB) is a source of human hematopoietic precursor cells (HPCs), a stem cell (SC) type that has been used in several trials for myocardial repair. A certain minimal number of cells is required for measurable regeneration and a major challenge of SC-based regenerative therapy constitutes ex-vivo expansion of the primitive cell c...

Phytoestrogens are widely used by postmenopausal women for the treatment of the climacteric syndrome. The risk of adverse effects of this treatment, however, is unknown. Using a fixed-effects model, we performed a meta-analysis of side effects comparing phytoestrogen treatment with placebo or no treatment in randomized controlled trials. We identif...

4-Hydroxyestrone (4-OHE(1)), a typical cancer-inducing metabolite, originating from 17beta-estradiol (17beta-E2), was chosen as a model for the studies. The aim was to get a deeper insight in the mechanisms of its ability to initiate cancer. It was found, that 4-OHE(1) can eject electrons (e(aq)(-)), when excited in the singlet state by monochromat...

Ovarian tissue cryopreservation (OTC) is not restricted to patients suffering from malignancies. Benign haematopoietic diseases such as autoimmune diseases and others have been mentioned to be feasible indications. The aim of our study was to demonstrate that surgery for OTC is a safe procedure for fertility preservation in patients with non-malign...

Sarcopenia, the age related decline in skeletal muscle mass has dramatic consequences. It leads to impaired performance, increased vulnerability, frailty and an increased risk of falls. Various extrinsic and intrinsic factors contribute to the aetiology of sarcopenia. The aims of the present study was to analyse gender differences in the prevalence...

To evaluate the incidence of venous thromboembolism (VTE) in transsexual patients and the value of screening for thrombophilia in this population. Retrospective cohort study. Academic research institution. Two hundred fifty-one transsexuals (162 male-to-female [MtF] and 89 female-to-male [FtM] transsexuals). Screening for activated protein C (aPC)...

Several genetic polymorphisms increase the risk for venous thrombembolism (VTE). In particular, combined oral contraceptives (COCs) are known to enhance the risk for VTE and are therefore contraindicated. We present here the case of a patient with protein S deficiency, who has used COCs together with anticoagulatory therapy (Phenprocoumon) after su...

Implanon has been reported to be effective in the treatment of dysmenorrhea. We compared the therapeutic efficacies of depot medroxyprogesterone acetate (DMPA) and Implanon with regard to pain relief in women with endometriosis. In a clinical research center at a university hospital, 41 patients with dysmenorrhea, nonmenstrual pelvic pain and dyspa...

Progesterone is known to be metabolized into various metabolites exerting various effects, predominantly into 5alpha-pregnanes and 4-pregnenes. Studies on uterine tissues showed numerous progesterone-converting enzymes such as 5alpha-reductase (5alphaR), 5beta-reductase, 3alpha-, 3beta-, 20alpha-, and 20beta-hydroxysteroid oxidoreductases and other...

Hintergrund: Drei Fragestellungen sollten in der Beantwortung des Karzinomrisikos bei assistierter Reproduktion (ART) berücksichtigt werden. (1) Sexualsteroide und Antiöstrogene spielen in der Entstehung und bei der Induktion des Wachstums gynäkologischer Karzinome wahrscheinlich eine große Rolle, obwohl die Zusammenhänge im Detail alles andere als...

Persistent Chlamydia trachomatis infections are associated with tubal pathology. We studied whether sampling from multiple sites would increase the identification of the infections. Prospective cohort study. Tertiary care facility. Two hundred two infertile women. Smears were taken from the cervix, urethra, high vagina, fimbriae and the Douglas cav...

Transsexualism is a rare condition. Although its etiology is unknown, it has been speculated that sex steroids may play an important role during early brain development. There may be a genetic component based on reports of an association between male to female (MtF) transsexualism and a CA repeat polymorphism in the estrogen receptor beta gene. CYP...

There is increasing awareness of the opportunity that many contraceptive interventions may provide for additional health benefits. However, treatment of medical problems with oral contraceptives (OCs) is often an 'off-label' practice. The aim of this review is to summarize available data on non-contraceptive benefits of OCs. Review of the literatur...

Besides the natural progestin, progesterone, there are different classes of progestins, such as retroprogesterone (i.e. dydrogesterone), progesterone derivatives (i.e. medrogestone) 17alpha-hydroxyprogesterone derivatives (i.e. chlormadinone acetate, cyproterone acetate, medroxyprogesterone acetate, megestrol acetate), 19-norprogesterone derivative...

It was established for the first time, that the sexual hormones 17beta-estradiol (17betaE2) and progesterone (PRG) are able to emit electrons from their excited single state in water-ethanol mixtures. The yield of the "solvated electrons" (e(s)(-)) depends on the substrate concentration, the ratio of water-alcohol-mixtures and the temperature. The...

To assess the association between transsexualism and allele and genotype frequencies of the common cytochrome P450 (CYP) 17 -34 T>C single nucleotide polymorphism (SNP). Case-control study. Academic research institution. 102 male-to-female (MtF) and 49 female-to-male (FtM) transsexuals, 756 male controls, and 915 female controls. Buccal swabs and m...

To systematically monitor the frequency and risk factors of adverse events (AEs) in a reproductive surgery endoscopy unit. Prospective cohort study. Academic research institution. All consecutive surgical patients of a reproductive surgery unit from December 2005 to March 2007. Monitoring for predefined AEs by trained observers. Number of preventab...

Recurrent miscarriage is known to affect 0.5-2% of pregnant women, and the standard investigative protocol fails to identify a specific cause in 50% of cases. Progesterone, a key hormone in pregnancy maintenance, has been used to support early pregnancy for decades. A growing body of considerable evidence indicates that in addition to women with lu...

BACKGROUND and objective: Many women of reproductive age experience depressive mood symptoms such as sudden mood swings, irritability, nervousness, excitability and anxiety. Although not defined as a disease, these disturbing mental symptoms are associated with a considerable decrease in quality of life. Molecular pharmacology research over the las...

Breast cancer and endometrial cancer are the most common gynecologic malignancies of the postmenopausal period. As preventive medicine becomes the focus of interest, preventive oncology with special regard to these diseases will undoubtedly become a substantial part of the practicing oncologist's field of duties. The aim of this review is to summar...

The aim of this study was to investigate the fertilization and pregnancy rates of artificial reproductive technologies using semen samples without (<1 x 10(6)/mL) and with (> or =1 x 10(6)/mL) leukocytospermia. The overall fertilization rate was 63.4% (range, 44.4-87.5%) in nonleukocytospermic couples and 64.3% (range, 45.3-100.0%) in leukocytosper...

The relation between genetic variation of the androgen metabolism and transsexualism is unknown. In a case-control study of 100 male-to-female (MtF) transsexuals, 47 female-to-male (FtM) transsexuals, and 1670 controls, the authors assess allele and genotype frequencies of the steroid 5-alpha reductase (SRD5A2) Val89Leu polymorphism using polymeras...

To investigate alterations in the overall gene expression profile of endometriosis-derived stroma with increasing concentrations of hCG by using the Affymetrix GeneChip U133 Set. In vitro study. Academic research institution. Women undergoing diagnostic laparoscopic surgery for endometriosis. Increasing concentrations of hCG, added to fibroblast mo...

To determine whether sex hormones alone or in combination with body mass index (BMI) influence mood in men. Blood samples were taken from 669 manual workers (aged 43-67 years) to measure sex hormone levels, in particular bioavailable testosterone (BAT). At the same time BMI was calculated. All participants completed the Beck's Depression Inventory...