Huan Xu

Huan Xu
Third Military Medical University | PLA · department of clinical laboratory

MD PhD Candidate

About

21
Publications
1,790
Reads
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318
Citations
Additional affiliations
September 2014 - June 2019
Third Military Medical University
Position
  • PhD Student

Publications

Publications (21)
Article
Water pollution and the global spread of pathogens pose unexpected and considerable challenges to human and environmental health. Recent developments in DNA-based engineering systems show various real-world applications in ecosystem restoration, medical diagnostics, immune regulation, and drug delivery, providing new opportunities to address these...
Article
Full-text available
Bacteriophage phiYY is currently the only double-stranded RNA (dsRNA) phage that infects Pseudomonas aeruginosa, and is a potential candidate for phage therapy. Here we applied RNA-seq to investigate the lytic cycle of phiYY infecting P. aeruginosa strain PAO1r. 12.45% (651/5229) of the host genes were determined to be differentially expressed gene...
Article
DNA nanomaterials are reliable and powerful tools in the development of a variety of biosensors owing to their notable self-assembly ability and precise recognition capability. Here, we propose a DNA nanomaterial-based system for the dual-amplified electrochemical sensing of circulating microRNAs by a coupled cascade of catalyzed hairpin assembly (...
Article
Point-of-care quantitative detection of biochemical markers has broad applications in resource-limited settings. However, current challenges including difficulty in sample preparation and limitation in result quantitation make point-of-care detection platforms failed to achieve fully sample-to-answer detection or meet the clinical quantitative dema...
Article
Full-text available
Point-of-care testing (POCT) has broad applications in resource-limited settings. Here, a POCT platform termed POCKET (point-of-care kit for the entire test) is demonstrated that is ultraportable and versatile for analyzing multiple types of DNA in different fields in a sample-to-answer manner. The POCKET is less than 100 g and smaller than 25 cm i...
Article
Early secreted antigenic target 6-kDa protein (ESAT6) is an essential virulence factor of Mycobacterium tuberculosis (MTb). However, ESAT6 helped fighting MTb infection according to vaccine studies. It's unclear whether ESAT6 confers protection via enhancing the innate immunity of macrophages, which are the first-line defense against MTb. We profil...
Article
There has been a constant and urgent need to upgrade the current diagnostic technologies to the next generation by utilizing innovative advanced technologies in order to meet new challenges. Micro/nano technology, a series of methods for design and manipulation materials at the micrometer and nanometer scales, which perfectly covers the key buildin...
Article
Problem: Systemic immuno-inflammatory response caused by maternal immune imbalance is central to the pathogenesis of preeclampsia (PE). We hypothesized that changes in the number of decidual mesenchymal stem cells (dMSCs) may be associated with maternal immune imbalance. We aimed to evaluate the expression of CXCL12/CXCR4 axis in patients with PE...
Article
A fast and simple strategy for early detection of circulating tumor cells (CTCs) is urgently required because of cancer metastasis. In this work, we assembled an electrochemical biosensor by two aptamers that could form hairpin and specifically recognize K562 cells. The thiolated capture aptamer was fixed on the gold electrode surface. The detectio...
Article
Full-text available
Cytometry is a basic method to determine cell populations and morphology. Flow cytometry and hemocytometry are the two most common methods among cytometric technologies. However, flow cytometry needs bulky and expensive equipment as well as professional operations, while hemocytometry is limited by its simple function. Both of them are not suitable...
Article
Objective: We aimed to identify plasma cytokine biomarkers that differentiate the infection stages of Mycobacterium tuberculosis (MTB). Methods: This study included a total of 227 subjects consisting of active tuberculosis (ATB) patients, latent tuberculosis infection (LTBI) individuals, and healthy controls (HC). We analyzed the expressions of...
Article
DNA nanomaterials have been widely used in bioassays due to their promising properties for sensitive and specific detection of biomolecules. Herein, a label-free electrochemical method was developed for quantitative detection of microRNAs by integrating Y-shaped DNA (Y-DNA) structures with non-linear hybridization chain reaction (non-linear HCR). T...
Article
Full-text available
Burkholderia thailandensis infection in humans is uncommon. We describe a case of B. thailandensis infection in a person in China, a location heretofore unknown for B. thailandensis. We identified the specific virulence factors of B. thailandensis, which may indicate a transition to a new virulent form. © 2017, Centers for Disease Control and Preve...
Data
Virulence factors of Burkholderia thailandensis strain BPM and results of computed tomography scan of patient’s chest and of bacterial cultures.
Article
Full-text available
Kruppel-like factor 2 (KLF2) is a putative tumor suppressor gene. This study investigated its role and epigenetic mechanisms in human non-small cell lung cancer (NSCLC) in ex vivo and in vitro. A total of 47 paired NSCLC and normal tissues and six cell lines were analyzed using qRT-PCR for KLF2 expression. KLF2 methylation was assessed using the me...
Article
Tumor-associated macrophages are a prominent component of lung cancer stroma and contribute to tumor progression. The protein V-set and Ig domain-containing 4 (VSIG4), a novel B7 family-related macrophage protein that has the capacity to inhibit T-cell activation, has a potential role in the development of lung cancer. In this study, 10 human non-s...
Article
TNF-α plays an essential role in the pathogenesis of fulminant virus hepatitis (FH) caused by infection with murine hepatitis virus strain-3 (MHV-3). However, the specific TNF-α receptors (TNFR) involved in this disease and how they mediate this effect are uncertain. Here, we showed that the expression of TNFR1 and TNFR2 in the liver and spleen was...
Article
Full-text available
It has been demonstrated that signals from the inhibitory receptor B and T lymphocyte attenuator (BTLA) are involved in regulating the pathogenesis of infectious diseases. However, the expression and anatomical distribution of BTLA and its ligand, the herpes virus entry mediator (HVEM), have not yet been determined in cases of HBV-related acute-on-...
Article
Hepatitis B virus (HBV) is a major public health problem, and HBV‐related acute‐on‐chronic liver failure (HBV‐ACLF) has an extremely poor prognosis due to a lack of effective treatments. B7‐H3 and B7‐H4 are two novel members of the B7 superfamily that are actively involved in regulating the pathogenesis of infectious diseases. However, the intrahep...
Article
Hepatitis B virus (HBV) infection is a major public health problem, and HBV-related acute-on-chronic liver failure (HBV-ACLF) has an extremely poor prognosis due to a lack of understanding of pathogenesis as well as a lack of effective treatments. Signals from the inhibitory receptor programmed death-1 (PD-1) have been demonstrated to be involved i...
Article
Objectives Fulminant viral hepatitis (FH) remains a serious clinical problem for which the underlying pathogenesis remains unclear. The B and T lymphocyte attenuator (BTLA) is an immunoglobulin-domain-containing protein that has the capacity to maintain peripheral tolerance and limit immunopathological damage during immune responses. However, its p...

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