Huai-Song Wang

China Pharmaceutical University | cpu · Department of Pharmaceutical Analysis

Nitrite, a type of food additive, has been proved convertible to genotoxic nitrosamines in the gastrointestinal tract by intestinal flora. There is no appropriate method for in situ detection of nitrosamines. Herein, plasmid-introduced Saccharomyces cerevisiae, which can respond to nitrosamine-induced DNA damage and activate pMAG1-based DNA damage...

Sialic acid (SA) is overexpressed on cell membranes of tumor cells, and increased serum SA concentration has been observed in tumor-bearing patients. Herein, a series of lanthanide-containing bimetallic complexes (TDA-M-Lns) for targeting SA were prepared via coordination among luminescent lanthanide ions (Ln3+ = Tb3+, Eu3+, Dy3+, or Sm3+), metal i...

Enrichment and detection of circulating free nucleic acids in biological samples have gained great attention for disease diagnosis or prognostic evaluation. Nanoscale metal-organic frameworks (NMOFs) have been used for aptamer-based nucleic acid sensing. In this work, different NMOFs, including ZIF-8, MIL-88, MIL-100, MIL-101, as well as Eu-TDA and...

Porous nanoscale coordination polymers (NCPs), self-assembled from metal ions and organic ligands, has rapidly progressed in the multidisciplinary areas (e.g. materials science, nanomedicine and nanotechnology) because of high porosity, large specific surface area and diversified structure. Researches focused on the combination of NCPs and biomedic...

Metal–organic frameworks (MOFs) are a class of hybrid materials assembled from metal ion/cluster connecting points and organic bridging ligands. On the basis of their properties of structural flexibility, porosity, controllable synthesis and flexibility of the pore size/wall modification, MOFs have shown great potential applications in biological s...

Homotypic targeting is the inherent ability of cells for preferential interaction with cells of similar or identical types, a phenomenon commonly seen in cell adhesion, tissue formation, and immune responses. Unfortunately, its full potential remains largely untapped. Here we introduce an approach to drastically boost the homotypic targeting capabi...

Let-7a, a type of low-expressed microRNAs in cancer cells, has been investigated as a promising biomarker and therapeutic target for tumor suppression. Developing simple and sensitive detection methods for let-7a is important for cancer diagnosis and treatment. In this work, the hybridization chain reaction (HCR) was initiated by let-7a via two hai...

As a class of widely used biocatalysts, enzymes possess advantages including high catalytic efficiency, strong specificity and mild reaction condition. However, most free enzymes have high requirements on the reaction environment and are easy to deactivate. Immobilization of enzymes on nanomaterial‐based substrates is a good way to solve this probl...

Drug resistance is a significant factor that hinders the success of cancer chemotherapy. The widely recognized mechanisms of drug resistance include changes to cell proliferation, cycle/apoptosis, drug metabolism/transport, DNA damage and the epithelial to mesenchymal transition. MicroRNAs (miRNAs), short non-coding RNAs with lengths of approximate...

The catalytic generation of H2 in living cells provides a method for antioxidant therapy. In this study, an [FeFe]-hydrogenase mimic [Ru+Fe2S2@F127(80)] was synthesized by self-assembling polymeric pluronic F-127, catalytic [Fe2S2] sites, and photosensitizer Ru(bpy)3²⁺. Under blue light irradiation, hydrated protons were photochemically reduced to...

Two kinds of Luminescent metal organic framework (LMOF) based probes were developed as fluorescent oligonucleotide indicators for ratio-metric microRNA (miRNA) sensing. One LMOF ([email protected]) is ZIF-8 encapsulated with gold nanoclusters (AuNCs). Another LMOF (Zn2Ph2Da) is prepared by using 2, 2'-dithiodibenzoic acid (dtba) and 1, 10-phenanthr...

Altering the glucose supply and the metabolic pathways would be an intriguing strategy in starvation therapy toward cancers. Nevertheless, starvation therapy alone could be inadequate to eliminate tumor cells completely. Herein, a multifunctional bioreactor was fabricated for synergistic starvation-chemotherapy through embedding glucose oxidase (GO...

Epithelial-mesenchymal transition (EMT) is implicated in the pathological processes of cancer metastasis and drug resistance. Anti-cancer drugs may also potentially lead to EMT, resulting in their reduced therapeutic effect. Therefore, the combination of these anti-cancer drugs with anti-EMT agents has been promoted in clinic. Screening anti-EMT dr...

A comprehensive undergraduate experiment about extraction, separation, and identification of essential oils (EOs) from a natural plant is described.Acori tatarinowiiRhizoma (ATR) was used as the raw plant material. Two devices (Soxhlet and Clevenger) with different extraction principles were employed for EO extraction from ATR, separately. The chem...

Bioorthogonal chemistry refers to any chemical reactions that can occur inside of living systems without interfering with native biochemical processes, which has become a promising strategy for modulating biological processes. The development of synthetic metal-based catalysts to perform bioorthogonal reactions has significantly expanded the toolki...

The diagnosis and therapy of tumors are challenging problems in the medical field. Peptides are derived from living organisms with excellent biocompatibility, low-toxicity/non-toxicity, and negligible immunogenicity, and they have been widely used in the field of biomedicine, especially in tumor sensing and treatment. In recent years, a series of t...

Tumor is a kind of abnormal organism generated by the proliferation and differentiation of cells in the body under the action of various initiating and promoting factors, which seriously threatens human life and health. Tumorigenesis is a gradual process that involves multistage reactions and the accumulation of mutations. Gene mutation usually occ...

Gold nanoclusters (AuNCs), with sizes of less than 2 nm, consist of several to hundreds of gold atoms. AuNCs, protected by templates or ligands, not only have high chemical stability, but also have high catalytic activity, high biocompatibility and strong luminescence. As a new type of fluorescent nanomaterial, AuNCs have large Stokes shift, long l...

Structure-efficacy effect of small molecular drug attracts wide attentions, but it has always been ignored in nanomedicine research. To reveal the efficacy modulation of nanomedicine, we developed a new type of paclitaxel (PTX)-conjugated gold nanoparticles (PTX-conjugated GNPs) to investigate the influence of drug position in controlling their in...

Metal-organic frameworks (MOFs) are built by various organic ligands and metal ions (or clusters). With properties of high porosity, tunable chemical composition, and potential for post-synthetic modification, they have been applied for biomedicine, especially in bio-sensing, bio-imaging, and drug delivery. Since organic ligands and metal centers (...

A novel nanoarchitecture (MSN-Tb-UbR) was prepared by modifying rhodamine-labelled Ubs (Ub-Rs) on the surface of mesoporous silica nanoparticle (MSN) loaded with Tb3+-complexes. The MSN-Tb-UbR exhibits ratiometric sensing ability for DUB...

Combination therapy has shown its promise in the clinic for enhancing the efficacy of tumor treatment. However, the dose control of multiple drugs and their non-overlapping toxicity from different drugs are still great challenge. In this work, a single model drug, paclitaxel (PTX), is used to realize combination therapy and solve the problems menti...

Metal-organic frameworks (MOFs), with diversify framework architectures, have evolved to be next-generation utility multifunctional hybrid materials. One key feature that MOFs possess is the luminescence properties which can be generated from building ligands, emissive metal ions, guest ions or molecules, as well as their catalytic activities. MOFs...

A new type of fluorescent coordination polymer (NCPCd) based on disulfide carboxylate ligand was prepared by using one-pot synthesis for sensitive detection of reactive oxygen species (ROS). With the reaction between NCPCd and ROS, the morphology of the NCPCd was transformed from nanorods to hexagon particles, then broken into nano-fragments. Meanw...

Mitochondria, as the energy supply factories for cell life activities, play important roles in controlling epigenetics, differentiation and initiation, and execution of apoptosis. These functions of mitochondria contribute to cell adaptation to challenging microenvironment conditions. In past decades, mitochondrial malfunctioning has been revealed...

Chitosan (CS), a kind of naturally produced polysaccharide with extraordinary biocompatibility and biodegradation, shows much potential to act as reducing and stabilizing agent in the synthesis of gold nanoparticles (AuNPs) for drug delivery. To solve the poor solubility and expand the pharmaceutical applications of CS, various CS derivatives throu...

Studies on the influence of one critical parameter (e.g., size), targeting a specific disease, while keeping other factors unchanged, are important for improving understanding and application of the molecular design of biomedical nanomaterials. In this study, we used doxorubicin (Dox)-conjugated gold nanoparticles (GNPs) to investigate the effects...

Metal-organic frameworks (MOFs) have been successfully used as efficient quenchers for fluorescent DNA detection. However, the surface charge property of MOFs can inevitably affect their fluorescence quenching behavior. Herein, nanoscale MOFs (NMOFs), including MOF nanosheets and nanoparticles, have been employed to investigate the relationship bet...

Co(2+) ions encapsulated in nitrogen doped graphene were applied as an oxygen evolution catalyst. Their redox potentials were tuned using different counter anions as liable ligands, and the redox potential related catalytic rates were explored. It was proposed that the electron density of Co(2+) ions was a general descriptor for activity.

Reactive oxygen species (ROS) are chemically reactive molecules that can mediate a wide variety of biological processes. The imbalance of these reactive intermediates in the metabolism will result in the phenomenon known as oxidative stress. Therefore, a great number of approaches have been developed for measuring ROS in biological systems. Due to...

A ligand exchange-based restricted access stationary phase was prepared via atom transfer radical polymerization. The stationary phase with bi-layer-polymer structure contains an inner layer for chiral separation and an outer layer for protein exclusion. L-Hydroxyproline was modified on the inner layer as chiral selector, and the benzene rings on t...

The high‑value applications of functional polymers in analytical science generally require well‑defined interfaces, including precisely synthesized molecular architectures and compositions. Controlled/living radical polymerization (CRP) has been developed as a versatile and powerful tool for the preparation of polymers with narrow molecular weight...

Two-dimensional (2D) materials have attracted tremendous interest as fluorescence quenchers of dye-labeled biomolecules for application in biosensing. Metal-organic framework (MOF) nanosheets, as a new type of 2D material, have rarely been studied as bioanalytical platforms. Herein, we synthesize a series of ultrathin lanthanide-based MOF (MOF-Ln)...

Novel biocompatible chiral monolithic stationary phase was prepared by reverse and direct atom transfer radical polymerization (ATRP) methods. By taking advantages of the controlled/living property of ATRP method, the chiral monolith was prepared by reverse ATRP (RATRP) firstly. An attractive feature of RATRP is the prepared polymer containing a te...

The major scientific challenge of enantiomeric separation is to develop simple, rapid and sensitive routine analytical methods. Generally, the enantio-resolution is still based on the ‘‘three-point interaction’’ theory, which indicates that homochiral sites are needed for enantio-selective interaction. In recent years, advanced materials with precis...

Oxidative stress caused by reactive oxygen species (ROS) is harmful to biological systems and implicated in various diseases. A variety of selective fluorescent probes have been developed for detecting ROS to uncover their biological functions. Generally, the preparation of the fluorescent probes usually undergo multiple synthetic steps, and the su...

In recent years, doped carbonaceous materials as alternative catalysts for oxygen reduction reaction (ORR) have received considerable attention due to the low cost and high CO tolerance capability. Different theoretical studies have suggested that oxygen is reduced in a rapid sequence intermediated by diverse oxygen-containing reactive intermediate...

Novel hydrophilic microparticles containing β-cyclodextrin (β-CD) were prepared via one-pot synthesis using reversible addition-fragmentation chain-transfer (RAFT) precipitation polymerization, a "controlled/living" radical polymerization technique. The polymerization was initiated by hydrophilic macromolecular chain-transfer agent [poly(2-hydroxye...

A micro-/nano-fluidics device integrating nanochannel in a microfluidics chip was developed for sensitively fluorescent determination of highly reactive oxygen species (hROS) enhanced by surface plasmon resonance-enhanced fluorescence (SPREF) effect. The nanochannel was simply fabricated by polyaniline nanostructures modified on a glass slide. Core...

A sensitive approach to the determination of reactive oxygen species (ROS) in puffs of cigarette smoke (CS) has been developed. The experimental system consists of a microfluidic chip electrophoresis and a laser induced fluorescence (LIF) device enhanced by localized surface plasmon resonance. Core-shell Ag@SiO2 nanoparticles were prepared and then...

A “comb-like” chiral stationary phase was developed using surface-initiated technique via atom transfer radical polymerization (ATRP). Chlorinated silica gel was produced as the ATRP initiator in a one-step reaction with thionyl chloride. This initiation method results in a hydrolytically stable initial Si–C bond for poly(glycidyl methacrylate) (pG...

An FTIR spectrum fitting algorithm based on continuous wavelet transform is proposed. In calculating the factor of difference spectrum, the algorithm takes into account both the original spectrum and its continuous wavelet transformed spectra, which effectively overcomes the problem of reference peak selection and manual factor selection in most co...

A new chiral stationary phase was designed and synthesized via surface-initiated atom-transfer radical polymerization (ATRP) and click chemistry. Poly(2-methyl-3-butyn-2-yl methacrylate-co-divinylbenzene), poly(MBMA-co-DVB), was grafted on silica surface via ATRP ("grafting-from" technique) first, and then azide-modified beta-CD was immobilized on...

A novel restricted access material was prepared by surface initiated atom transfer radical polymerization. The bi-layer-polymer structures were created on the surface of silica layer-by-layer. The inner layer was composed of poly(styrene-co-divinylbenzene), which was grafted first for binding small molecules based on hydrophobic and π-π interaction...

A quantitative calibration model for Infrared spectroscopy using continuous wavelet transform combined with genetic algorithm is presented in this paper. We propose three scale selection methods in continuous wavelet transform and provide the comparisons with the general preprocessing methods. Experimental results show that selectively combining sc...

A novel chiral stationary phase was synthesized via the combination of atom transfer radical polymerization (ATRP) and click chemistry. In the synthesis, the silane coupling agent 3-(2-bromoisobutyryl)-propyl triethoxysilane (BPE) was chosen as the ATRP initiator and immobized on the porous silica gel. The polymer chains of poly(2-methyl-3-butyn-2-...

A novel chiral restricted access material was synthesized via a combination of atom transfer radical polymerization (ATRP) and click chemistry. Poly(2-methyl-3-butyn-2-ol methacrylate) (pMBMA) was grafted onto porous silica gel by a surface-initiated ATRP in order to synthesize an inner layer for β-cyclodextrin (β-CD) immobilization. The azide-modi...

The development of new separation materials to meet growing demands has always been a major concern of separation science and technology. Controlled/living radical polymerization (CRP) is a technique with many advantages for polymer synthesis. Polymers with low polydispersities and desirable architectures can be prepared using this technique. The a...

A novel restricted access-molecularly imprinted material (RA-MIP) with selectivity for sulfonamides was synthesized using initiator-transfer agent-terminator (iniferter) method, a "living"/controlled radical polymerization technique. The material was prepared by grafting two layers with different functions on the silica support. To perform a "graft...

A new restricted access chiral stationary phase was designed and synthesized via atom transfer radical polymerization (ATRP). Surface modified bi-functional silica with chiral selector in the inner layer and hydrophilic external layer was prepared by taking advantage of the controlled/living property of the ATRP method. ATRP initiator bound porous...