Hristina VasilevaLondon School of Hygiene and Tropical Medicine | LSHTM
Hristina Vasileva
MSc Medical Microbiology
About
8
Publications
2,658
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77
Citations
Citations since 2017
Introduction
Hristina Vasileva currently works and undertakes a part-time PhD at the Clinical Research Department and the Department of Infectious Biology, at the faculty of Infectious Tropical Diseases, LSHTM. Hristina does research in Molecular Biology, Microbiology and Genetics, working on human host response to malaria infection or exposure. Her PhD project is focused in understanding the understudied hypervariable surface proteins on P. falciparum and their role in malaria immunity and exposure.
Additional affiliations
October 2017 - October 2019
Publications
Publications (8)
Ivermectin, used to control several neglected tropical diseases (NTDs), may also reduce malaria transmission. Mass drug administration (MDA) for malaria control therefore might have off-target impacts on NTDs.
Methods
In the Gambia, nested in a trial of ivermectin MDA, cross-sectional surveys measuring ectoparasites and soil-transmitted helminths...
Background
The prevalence of sexually transmitted infections (STIs) in sub-Saharan Africa is poorly described. We aimed to determine the prevalence of five curable STIs (Chlamydia trachomatis [CT], Neisseria gonorrhoeae [NG], Trichomonas vaginalis [TV], Mycoplasma genitalium [MG], Treponema pallidum [TP]) in a sample of Gambian women from the gener...
Distribution of long-lasting insecticide-treated nets (LLINs), passive detection and treatment with artemisinin-based combination therapy (ACT), and intermittent preventive treatment in pregnancy (IPTp) are the mainstay malaria control measures of Guinea-Bissau’s national control programme. This study aimed to estimate the prevalence of Plasmodium...
Background
The presence of Chlamydia trachomatis (Ct) DNA at non-ocular sites suggests that these sites may represent plausible routes of Ct transmission in trachoma. However, qPCR cannot discriminate between DNA from viable and non-viable bacteria. Here we use a propodium monoazide based viability PCR to investigate how long Ct remains viable at n...
Objectives
Complications from sexually transmitted infections (STIs) can result in severe morbidity and mortality. To date, no STI population studies have been conducted on the Bijagos Islands, Guinea Bissau. Our objective was to estimate the prevalence of and identify risk factors for Chlamydia trachomatis (Ct), Neisseria gonorrhoea (Ng), Mycoplas...
Background: Antibody responses have been used to characterise transmission and exposure history in malaria-endemic settings for over a decade. Such studies have typically been conducted on well-standardised enzyme-linked immunosorbent assays (ELISAs). However, recently developed quantitative suspension array technologies (qSAT) are now capable of h...
Background: Antibody responses have been used to characterise transmission and exposure history in malaria-endemic settings for over a decade. Such studies have typically been conducted on well-standardised enzyme-linked immunosorbent assays (ELISAs). However, recently developed quantitative suspension array technologies (qSAT) are now capable of h...
Background
Clinical signs of active (inflammatory) trachoma are found in many children in the Solomon Islands, but the majority of these individuals have no serological evidence of previous infection with Chlamydia trachomatis. In Temotu and Rennell and Bellona provinces, ocular infections with C. trachomatis were seldom detected among children wit...
Questions
Questions (2)
Hi,
I have been trying to optimize a viability PCR using propidium monoazide (PMA) chemical. The idea is heat killing bacterial samples treating them with PMA and comparing the qRT-PCR results to non heat killed bacterial samples. PMA is supposed to bind to DNA and would inhibit the PCR of the bacteria. Also it is expected that PMA is non-membrane permeable, thus it will bind only to dead and/or membrane disturbed cells.
In reality one would expect less bacterial detection (if any) when the samples were killed compared to non-killed one. However I have been getting exactly the opposite results for almost a month now. I changed the PMA stock, tested different concentration, also tested on different life bacteria concentrations, time of PMA exposure, human error (made a more experienced colleague to perform the experiment) and I have been getting the same results.
Does anyone had problems in working with vPCR before? And if you have suggestions on why I am getting those results and what else I can do to find out where things are getting wrong, if will be very helpful.
Thank you.
I have done a transcriptome analysis using microarray gene chips HTA 2.0. My study has a comparative part with another study, which have been using U133+2 chips so I could only compare the end results and interpretations of the 2 studies.
I would like to be able to use the raw data from both study and normalize and filter them together for more reliable results.
Any idea how I can do so?
Projects
Projects (2)
To determine the exposure and transmission patterns of various neglected tropical diseases (NTDs), such as lymphatic filariasis, soil transmitted helminths, yaws etc. To investigate the serological responses against Malaria in an asymptomatic population. To investigate the serological profile against Trachoma in a population declared Trachoma free.
Investigating means of transmission of ocular Chlamydia trachomatis strains in Ethiopia.
