Houssam Attoui

Houssam Attoui
French National Institute for Agriculture, Food, and Environment (INRAE) | INRAE · Department of Animal Health (Virology)

PharmD, MSc, PhD, HDR

About

276
Publications
25,703
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7,291
Citations
Additional affiliations
December 2015 - present
French National Institute for Agriculture, Food, and Environment (INRAE)
Position
  • Director of research/Research Leader
June 2006 - December 2015
The Pirbright Institute
Position
  • Senior Scientist/Research Leader
June 1998 - December 2006
Etablissement Français du Sang (EFS)
Position
  • Senior Scientist/Research Leader

Publications

Publications (276)
Article
Full-text available
Tick-borne viruses are responsible for various symptoms in humans and animals, ranging from simple fever to neurological disorders or haemorrhagic fevers. The Kemerovo virus (KEMV) is a tick-borne orbivirus, and it has been suspected to be responsible for human encephalitis cases in Russia and central Europe. It has been isolated from Ixodes persul...
Article
Full-text available
Up to 170 tick-borne viruses (TBVs) have been identified to date. However, there is a paucity of information regarding TBVs and their interaction with respective vectors, limiting the development of new effective and urgently needed control methods. To overcome this gap of knowledge, it is essential to reproduce transmission cycles under controlled...
Article
Full-text available
Up to 170 tick-borne viruses (TBVs) have been identified to date. However, there is a paucity of information regarding TBVs and their interaction with respective vectors, limiting the development of new effective and urgently needed control methods. To overcome this gap of knowledge, it is essential to reproduce transmission cycles under controlled...
Article
Full-text available
Bluetongue virus serotypes 1 to 24 are transmitted primarily by infected Culicoides midges, in which they also replicate. However, “atypical” BTV serotypes (BTV-25, -26, -27 and -28) have recently been identified that do not infect and replicate in adult Culicoides, or a Culicoides derived cell line (KC cells). These atypical viruses are transmitte...
Article
Full-text available
Bluetongue (BT) is a severe and economically important disease of ruminants that is widely distributed around the world, caused by the bluetongue virus (BTV). More than 28 different BTV serotypes have been identified in serum neutralisation tests (SNT), which, along with geographic variants (topotypes) within each serotype, reflect differences in B...
Article
Full-text available
Statin derivatives can inhibit the replication of a range of viruses, including hepatitis C virus (HCV, Hepacivirus), dengue virus (Flavivirus), African swine fever virus (Asfarviridae) and poliovirus (Picornaviridae). We assess the antiviral effect of fluvastatin in cells infected with orbiviruses (bluetongue virus (BTV) and Great Island virus (GI...
Article
Although tick-borne infectious diseases threaten human and animal health worldwide, with constantly increasing incidence, little knowledge is available regarding vector-pathogen interactions and pathogen transmission. In vivo laboratory study of these subjects using live, intact ticks is expensive, labor-intensive, and challenging from the points o...
Article
Full-text available
Ticks and tick-borne diseases (TBDs) represent a burden for human and animal health worldwide. Currently, vaccines constitute the safest and most effective approach to control ticks and TBDs. Subolesin (SUB) has been identified as a vaccine antigen for the control of tick infestations and pathogen infection and transmission. The characterization of...
Article
Full-text available
Background Louping ill virus (LIV) and tick-borne encephalitis virus (TBEV) are tick-borne flaviviruses that are both transmitted by the major European tick, Ixodes ricinus. Despite the importance of I. ricinus as an arthropod vector, its capacity to acquire and subsequently transmit viruses, known as vector competence, is poorly understood. At the...
Preprint
Full-text available
Background Louping ill virus (LIV) and tick-borne encephalitis virus (TBEV) are tick-borne flaviviruses that are both transmitted by the major European tick, Ixodes ricinus . Despite the importance of I. ricinus as an arthropod vector, its capacity to acquire and subsequently transmit viruses, known as vector competence, is poorly understood. At th...
Chapter
Animal “arboviruses” include viruses that are transmitted between their vertebrate hosts by arthropod vectors. They replicate in both their haematophagous arthropod vectors and vertebrate hosts. Vectors become infected after feeding on infected hosts. After replication and dissemination to the vector’s salivary glands, the virus can be transmitted...
Article
Full-text available
Animal arboviruses replicate in their invertebrate vectors and vertebrate hosts. They use several strategies to ensure replication/transmission. Their high mutation rates and propensity to generate recombinants and/or genome segment reassortments help them adapt to new hosts/emerge in new geographical areas. Studying arbovirus genetic variability h...
Article
Full-text available
Regulatory factors controlling tick salivary glands (SGs) are direct upstream neural signaling pathways arising from the tick’s central nervous system. Here we investigated the cholinergic signaling pathway in the SG of two hard tick species. We reconstructed the organization of the cholinergic gene locus, and then used in situ hybridization to loc...
Article
Full-text available
Regulatory factors controlling tick salivary glands (SGs) are direct upstream neural signaling pathways arising from the tick’s central nervous system. Here we investigated the cholinergic signaling pathway in the SG of two hard tick species. We reconstructed the organization of the cholinergic gene locus, and then used in situ hybridization to loc...
Article
Full-text available
Culicoides biting midges (Diptera: Ceratopogonidae) transmit arboviruses of veterinary or medical importance, including bluetongue virus (BTV) and Schmallenberg virus, as well as causing severe irritation to livestock and humans. Arthropod cell lines are essential laboratory research tools for the isolation and propagation of vector-borne pathogens...
Chapter
The family Reoviridae contains 15 genera with the genus Orbivirus being the largest of the currently classified ones. It contains Bluetongue virus (the Orbivirus type species), 27 virus species with bluetongue virus as type species and (at least) 8 currently unclassified ‘orbiviruses’. With a few exceptions, the orbiviruses infect, replicate in and...
Article
Full-text available
Bluetongue is a severe, economically important disease of ruminants that is widely distributed in tropical and temperate regions around the world. It is associated with major production losses, restrictions of animal movements and trade, as well as costs associated with developing and implementing effective surveillance and control measures. Mammal...
Article
Full-text available
Mosquito-borne viruses are the cause of some of the greatest burdens to human health worldwide, particularly in tropical regions where both human populations and mosquito numbers are abundant. Due to a combination of anthropogenic change, including the effects on global climate and wildlife migration there is strong evidence that temperate regions...
Article
Full-text available
Birnaviridae is a family of viruses with bi-segmented dsRNA genomes totalling about 6 kbp forming icosahedral, non-enveloped virions. The family includes four genera, members of three of which (Aquabirnavirus, Avibirnavirus and Blosnavirus) infect vertebrates (excluding mammals), whereas members of the fourth genus (Entomobirnavirus) infect insects...
Article
Full-text available
Picobirnaviridae is a family of viruses with bi-segmented (rarely unsegmented) dsRNA genomes comprising about 4.4 kbp in total, with small, non-enveloped spherical virions. The family includes one genus (Picobirnavirus) grouping three genetic clusters with high sequence variability, two defined by viruses infecting vertebrates and a third with viru...
Article
Bluetongue virus (BTV) hitherto consisted of 26 recognized serotypes, of which all except BTV-26 are primarily transmitted by certain species of Culicoides biting midges. Three variants of an additional 27th bluetongue virus serotype (BTV-27v01-v03) were recently detected in asymptomatic goats in Corsica, France, 2014-2015. Molecular characterizati...
Chapter
Bluetongue virus (BTV) is the type species of genus Orbivirus within family Reoviridae. Bluetongue virus is transmitted between its ruminant hosts by the bite of Culicoides spp. midges. Severe BT cases are characterized by symptoms including hemorrhagic fever, particularly in sheep, loss of productivity, and death. To date, 27 BTV serotypes have be...
Book
Chapter Seven Guillaume Belbis et Stephan Zientara sont co-premiers auteurs.
Article
Full-text available
While ticks have been known to harbor and transmit pathogenic arboviruses for over 80 years, the application of high-throughput sequencing technologies has revealed that ticks also appear to harbor a diverse range of endogenous tick-only viruses belonging to many different families. Almost nothing is known about these viruses; indeed, it is unclear...
Article
Full-text available
We announce here the complete genome sequence (coding and noncoding) of the bluetongue virus (BTV) serotype 8, isolated from a ram in Allier department, France, 2015. Sequence analysis confirms the reemergence of the BTV-8 strain that previously circulated in France until 2009 and other European countries until 2010.
Article
Full-text available
Bluetongue virus (BTV) can infect most ruminant species and is usually transmitted by adult, vector-competent biting midges (Culicoides spp.). Infection with BTV can cause severe clinical signs and can be fatal, particularly in naïve sheep and some deer species. Although 24 distinct BTV serotypes were recognized for several decades, additional 'typ...
Chapter
Viruses of the genus Coltivirus are members of family Reoviridae. Their polysegmented genome consists of 12 linear segments of dsRNA. They are tick-transmitted viruses that can infect humans. Coltivirus isolations have been described from the northwestern part of the USA, Canada, and Europe. The viruses identified in the USA are members of the spec...
Article
Since 1983, cases of diseased donkeys and horses with symptoms similar to those produced by alphaviruses were identified in two departments in northern Peru; however serological testing ruled out the presence of those viruses and attempts to isolate an agent were also unproductive. In 1997, also in northern Peru, two new orbiviruses were discovered...
Article
Full-text available
Many novel emerging orbiviruses have been isolated inthe past 15years. Important viruses include Peruvian horse sickness virus (PHSV) and Yunnan orbivirus (YUOV), pathogens of equids which were originally isolated almost simultaneously from 1997 to 1999 in the People's Republic of China, Australia and Peru. YUOV has also been isolated from cattle,...
Article
Full-text available
Since 1998 there have been significant changes in the global distribution of bluetongue virus (BTV). Ten previously exotic BTV serotypes have been detected in Europe, causing severe disease outbreaks in naïve ruminant populations. Previously exotic BTV serotypes were also identified in the USA, Israel, Australia and India. BTV is transmitted by bit...
Chapter
Coltiviruses and seadornaviruses are members of the family Reoviridae, which encompasses alongside the genera Coltivirus and Seadornavirus, 13 other genera of viruses that can infect mammals, birds, aquatic animals, reptiles, insects, plants, fungi and protists. Coltiviruses are transmitted by hard ticks of the family Ixodidae, whereas seadornaviru...
Chapter
Bluetongue virus (BTV) is the type species of the genus Orbivirus within the family Reoviridae (the largest double-stranded ribonucleic acid (dsRNA) virus family). BTV is an arthropod-borne pathogen that is transmitted by the bites of its vector, the Culicoides biting midges, which transmit the virus between its ruminant hosts. The virus can infect...
Article
Full-text available
The International Committee for Taxonomy of Viruses (ICTV) recognizes four species of tick-borne orbiviruses (TBOs): Chenuda virus, Chobar Gorge virus, Wad Medani virus and Great Island virus (genus Orbivirus, family Reoviridae). Nucleotide (nt) and amino acid (aa) sequence comparisons provide a basis for orbivirus detection and classification, how...
Chapter
The orbiviruses are ten-segmented double-stranded RNA viruses that comprise the largest genus within the family Reoviridae, which is itself one of the largest and most diverse families of the RNA viruses. The orbiviruses are arthropod-borne viruses (arboviruses) that are transmitted between vertebrate hosts via the bite of hematophagous arthropods,...
Article
Full-text available
Viruses belonging to the species Wallal virus and Warrego virus of the genus Orbivirus were identified as causative agents of blindness in marsupials in Australia during 1994/5. Recent comparisons of nucleotide (nt) and amino acid (aa) sequences have provided a basis for the grouping and classification of orbivirus isolates. However, full-genome se...
Article
Full-text available
BTV-4 structural proteins VP2 (as two domains: VP2D1 and VP2D2), VP5 (lacking the first 100 amino acids: VP5Δ1-100) and full-length VP7, expressed in bacteria as soluble glutathione S-transferase (GST) fusion-proteins, were used to immunise Balb/c and α/β interferon receptor knock-out (IFNAR(-/-)) mice. Neutralising antibody (NAbs) titres (expresse...
Data
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Article
Full-text available
The complete genomes of Orungo virus (ORUV), Lebombo virus (LEBV) and Changuinola virus (CGLV) were sequenced, confirming that they each encode 11 distinct proteins (VP1-VP7 and NS1-NS4). Phylogenetic analyses of cell-attachment protein 'outer-capsid protein 1' (OC1), show that orbiviruses fall into three large groups, identified as: VP2(OC1), in w...
Article
Full-text available
The species Corriparta virus (CORV), within the genus Orbivirus, family Reoviridae, currently contains six virus strains: corriparta virus MRM1 (CORV-MRM1); CS0109; V654; V370; Acado virus and Jacareacanga virus. However, lack of neutralization assays, or reference genome sequence data has prevented further analysis of their intra-serogroup/species...
Article
Full-text available
Ticks transmit a wide range of viral, bacterial and protozoan pathogens, many of which can establish persistent infections of lifelong duration in the vector tick and in some cases are transmitted transovarially to the next generation. In addition many ixodid and argasid tick cell lines and, by inference the parent ticks from which they were derive...
Article
Full-text available
Background The recent unprecedented emergence of arboviruses transmitted by Culicoides biting midges in northern Europe has necessitated the development of techniques to differentiate competent vector species. At present these techniques are entirely reliant upon interpretation of semi-quantitative RT-PCR (sqPCR) data in the form of Cq values used...
Data
Full-text available
OpenBUGS code for implementing the two-component mixture model. (PDF)
Data
Marginal posterior densities for parameters in a two-component mixture model, (1), for (A,C,E,G) C. sonorensis and (B,D,F,H) C. nubeculosus. (A,B) Probability of developing a transmissible infection. (C,D) Mean and (E,F) standard deviation of the Cq values for Culicoides with a transmissible (solid line) or a sub-transmissible (dashed line) infecti...
Data
Comparison of the observed (bars) and expected (posterior mean (circles) and 95% prediction intervals (error bars)) Cq values for (A) C. sonorensis and (B) C. nubeculosus infected by feeding on SBV-infected blood via a membrane. (TIF)
Data
Comparison of posterior predictions of the mixture model, (1), based on data for (A,B) C. sonorensis or (C,D) C. nubeculosus processed as whole insects and the results for dissected individuals. (A,C) Predicted number of Culicoides with a transmissible infection following membrane feeding. The bars indicate the relative frequency and the arrow the...
Article
Full-text available
To the Editor: After unexpected emergence of bluetongue virus serotype 8 in northern Europe in 2006 (1), another arbovirus, Schmallenberg virus (SBV), which is transmitted by Culicoides spp. biting midges, emerged in Europe in 2011 and caused disease outbreaks among ruminants (2). Nonspecific clinical signs such as fever, decreased milk production,...
Article
Full-text available
Liao ning virus (LNV) is related to Banna virus, a known human-pathogen present in south-east Asia. Both viruses belong to the genus Seadornavirus, family Reoviridae. LNV causes lethal haemorrhage in experimentally infected mice. Twenty seven isolates of LNV were made from mosquitoes collected in different locations within the Xinjiang province of...
Article
Full-text available
Eubenangee virus has previously been identified as the cause of Tammar sudden death syndrome (TSDS). Eubenangee virus (EUBV), Tilligery virus (TILV), Pata virus (PATAV) and Ngoupe virus (NGOV) are currently all classified within the Eubenangee virus species of the genus Orbivirus, family Reoviridae. Full genome sequencing confirmed that EUBV and TI...
Data
Nuclear localization signals (NLS) in NS4 of Eubenangee, Tilligerry and Pata viruses. (DOCX)
Article
This paper reports a concatemeric RNA in a strain of epizootic haemorrhagic disease virus (EHDV) serotype 5. Sequencing showed that the concatemeric RNA contains two identical full-length copies of genome segment 9, arranged in series, which has apparently replaced the monomeric form of the segment. In vitro translation demonstrated that the concat...
Article
Full-text available
Bluetongue virus is the "type" species of the genus Orbivirus, family Reoviridae. Twenty four distinct bluetongue virus (BTV) serotypes have been recognized for decades, any of which is thought to be capable of causing "bluetongue" (BT), an insect-borne disease of ruminants. However, two further BTV serotypes, BTV-25 (Toggenburg orbivirus, from Swi...
Article
Full-text available
The genus Orbivirus includes both insect and tick-borne viruses. The orbivirus genome, composed of 10 segments of dsRNA, encodes 7 structural proteins (VP1-VP7) and 3 non-structural proteins (NS1-NS3). An open reading frame (ORF) that spans almost the entire length of genome segment-9 (Seg-9) encodes VP6 (the viral helicase). However, bioinformatic...
Data
Nucleotide accession numbers for sequences used in phylogenetic analysis. (DOC)
Data
Percent identity of UMAV and SLOV Polymerase (Pol) with other orbiviruses. (DOC)
Data
Identity levels in the outer-core protein VP7(T13) and gene of UMAV, compared to other orbiviruses. (DOC)