Horia Banciu

Horia Banciu
Babeş-Bolyai University | UBB · Department of Molecular Biology and Biotechnology

Professor

About

120
Publications
23,275
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,326
Citations
Introduction
My main research interests are focused on diversity and physiology of halophilic microorganisms, and ecology of saline and hypersaline aquatic systems.

Publications

Publications (120)
Article
Full-text available
Members of candidate Asgardarchaeota superphylum appear to share numerous eukaryotic-like attributes thus being broadly explored for their relevance to eukaryogenesis. On the contrast, the ecological roles of Asgard archaea remains understudied. Asgard archaea have been frequently associated to low-oxygen aquatic sedimentary environments worldwide...
Poster
Full-text available
Here we aimed at evaluating the diversity, abundance, and organic substrate preferences of microbial communities associated with sediments and water from a pristine karst cave located in Romania. Methods: Culture-independent techniques were employed to evaluate bacterial diversity, for which total environmental DNA (eDNA) was extracted using the Qu...
Article
During our initial phylogenetic study of the monocot genus Erythronium (Liliaceae), we observed peculiar eudicot‐type internal transcribed spacer (ITS) sequences in a dataset derived from genomic DNA of Erythronium dens‐canis. This raised the possibility horizontal transfer of a eudicot alien ribosomal DNA (rDNA) into the Erythronium genome. In thi...
Preprint
Full-text available
The occurrence of horizontal gene transfer (HGT) in Eukarya is increasingly gaining recognition. Nuclear-to-nuclear jump of DNA between plant species at high phylogenetic distance and devoid of intimate association (e.g., parasitism) is still scarcely reported. Within eukaryotes, components of ribosomal DNA (rDNA) multigene family have been found t...
Poster
Full-text available
Abstract: Movile Cave (Romania) is a chemoautotrophic-based subterranean environment that hosts a diverse invertebrate community, apparently isolated from the Earth’s surface. A sulfidic thermal aquifer provides the energy for the primary producers of the ecosystem. To identify the microbial players of the ecosystem and understand their ecological...
Article
Full-text available
The genetic signature of modern Europeans is the cumulated result of millennia of discrete small-scale exchanges between multiple distinct population groups that performed a repeated cycle of movement, settlement, and interactions with each other. In this study we aimed to highlight one such minute genetic cycle in a sea of genetic interactions by...
Chapter
The organic‐rich sediments (or “sapropels”) are complex organo‐mineral and microoxic/anoxic milieus underlying stagnant aquatic basins with high input of organic matter in addition to persistent salinity and density gradients. Sapropels of brackish, marine and hypersaline aquatic systems were found to harbor one of the most diverse microbiomes repo...
Article
Full-text available
Introduction: Karstic springs are used worldwide by rural communities as sources of fresh water for humans and livestock. In Romania, one-third of the population has no direct access to a public water supply. The present study is part of a country-wide project to develop simple, quick and cheap methods for seasonal environmental and microbiological...
Preprint
Full-text available
(1) Introduction: Karstic springs are used worldwide by rural communities as sources of fresh water for human use and livestock survival. In Romania, 1/3 of the population has no direct access to the public water supply. The present study is part of a country-wide project on developing simple, quick and cheap methods for seasonal environmental and...
Article
Full-text available
Asgardarchaeota are considered to be the closest relatives to modern eukaryotes. These enigmatic microbes have been mainly studied using metagenome-assembled genomes (MAGs). Only very recently, a first member of Lokiarchaeia was isolated and characterized in detail; it featured a striking morphology with long, branching protrusions. In order to vis...
Article
Full-text available
Schizorhodopsins (SzRs), a rhodopsin family first identified in Asgard archaea, the archaeal group closest to eukaryotes, are present at a phylogenetically intermediate position between typical microbial rhodopsins and heliorhodopsins. However, the biological function and molecular properties of SzRs have not been reported. Here, SzRs from Asgardar...
Article
Full-text available
Ursu Lake is located in the Middle Miocene salt deposit of Central Romania. It is stratified, and the water column has three distinct water masses: an upper, freshwater‐to‐moderately saline stratum (0–3 m), an intermediate stratum exhibiting a steep halocline (3–3.5 m), and a lower, hypersaline stratum (4 m and below) that is euxinic (i.e. anoxic a...
Article
Full-text available
In the past 100 years, a decreasing rainfall trend has been recorded on Romanian territory, a trend that continues today. Therefore, realistic estimation of the groundwater resources is crucial, especially for the rural communities lacking the economic power to use alternative sources of drinking water. The groundwater sources used by rural communi...
Article
Full-text available
In June 2019, the 12th International Conference on Halophilic Microorganisms - Halophiles 2019, was held in Cluj-Napoca, Romania. This thematic issue of FEMS Microbiology Letters contains papers based on lectures and posters presented at the conference. We here provide a short overview of past research on hypersaline environments in Romania and the...
Article
Adaptive strategies responsible for heavy metal tolerance were explored in the extremely halophilic archaeon Halomicrobium mukohataei DSM 12286. The tested strain was seemingly able to overcome silver-induced oxidative stress (assessed by malondialdehyde quantification, catalase assay and total antioxidant capacity measurement) mainly through non-e...
Article
Full-text available
Recent advances in phylogenomic analyses and increased genomic sampling of uncultured prokaryotic lineages have brought compelling evidence in support of the emergence of eukaryotes from within the archaeal domain of life (eocyte hypothesis)1,2. The discovery of Asgardarchaeota and its supposed position at the base of the eukaryotic tree of life3,4...
Conference Paper
Full-text available
Saline aquatic systems include inland and marine-derived lakes, solar salterns, or temporary inland saline ponds. Despite their high salinity, these ecosystems are inhabited by diverse microbial communities driving full biogeochemical cycling of main elements. To date, numerous salt-loving (or ‘halophilic’) environmental strains with metabolic trai...
Conference Paper
Full-text available
The origin of the eukaryotic cell and its shared evolutionary history with Archaea are among the hottest topics in modern biology. Recent improvements in culture-independent genomics and phylogenomic analyses provided compelling evidence in support of the emergence of eukaryotes from within the Archaea. An important step towards revealing the ident...
Preprint
Full-text available
Metagenome-assembled genomes (MAGs) of Asgardarchaeota are starting to be recovered from a variety of habitats, broadening their environmental distribution and providing access to the genetic makeup of this archaeal lineage. Despite their singular phylogenetic position at the base of the eukaryotic tree of life, the morphology of these bewildering...
Article
Full-text available
Air microbial pollution in touristic areas poses a risk for both the integrity of an ecosystem and human health. Microbiological monitoring together with environmental parameters monitoring allows for the assessment of the impacts and formulation of sound management decisions to protect humans and ecosystems. Four show caves from the Carpathian Mou...
Preprint
Full-text available
Recent advances in phylogenomic analyses and increased genomic sampling of uncultured prokaryotic lineages brought compelling evidence in support of the emergence of eukaryotes from within the Archaea domain of life. The discovery of Asgardaeota archaea and their recognition as the closest extant relative of eukaryotes fuelled the revival of a deca...
Article
Full-text available
Aim This study aimed at unprecedented physical and chemical evaluation of the ‘green plastics’ polyhydroxyalkanoates (PHAs), in an extremely halotolerant Halomonas elongata strain 2FF under high salt concentration. Methods and results The investigated bacterial strain was isolated from the surface water of the hypersaline Fără Fund Lake. The 16S r...
Article
Full-text available
NaCl-saturated brines such as saltern crystallizer ponds, inland salt-lakes, deep-sea brines, and liquids-of-deliquescence on halite are commonly regarded as a paradigm for the limit-of-life on Earth. There are, however, other habitats that are thermodynamically more extreme. Typically, NaCl-saturated environments contain all domains-of-life and pe...
Conference Paper
Full-text available
Organic-rich sediments (or ‘sapropels’) are complex organo-mineral matrices that form at the O2-depleted bottom of stagnant water basins. Besides geochemical transformations, slow rate microbially-driven processes underpin the early diagenesis of these sediments. The fossil sapropels found in various marine or lacustrine environments are known as p...
Poster
Full-text available
Preliminary data on invertebrate diversity of 19 brackish, moderately saline and hypersaline lakes from Transylvania. First results on zooplankton community.
Article
Full-text available
In the present work, we review the current knowledge on genesis, limnology and biodiversity of salt lakes distributed around the inner contour of Eastern Carpathian arc (Transylvanian Basin, Central Romania). Transylvanian salt lakes formed on ancient halite (NaCl) deposits following natural processes or quarrying activities. Most of these lakes ar...
Article
Full-text available
Present-day terrestrial analogue sites are crucial ground truth proxies for studying life in geochemical conditions close to those assumed to be present on early Earth or inferred to exist on other celestial bodies (e.g. Mars, Europa). Although hypersaline sapropels are border-of-life habitats with moderate occurrence, their microbiological and phy...
Article
Full-text available
In this study, we aimed at developing a rapid and non-destructive method to predict the genus-level taxonomic affiliation of phenotypically similar halophilic archaea of Halobacteria Class. For this purpose, representatives of three widespread and frequently isolated haloarchaeal genera (Halobacterium sp., Haloferax sp. and Halorubrum sp.) were inv...
Article
Full-text available
In this study, we investigated the biomineralization potential and diversity of the epilithic bacterial communities dwelling on the limestone statue of Saint Donatus, the oldest public monument of Cluj-Napoca city (Transylvania region, NW Romania). Their spatial distribution together with phylogenetic and metabolic diversity, as well as their capac...
Article
Background: Colorado potato beetle (CPB) has become the biggest enemy of cultivated potato worldwide. One of the most effective sources of resistance to CPB is Solanum chacoense, an accession with a high leptine glycoalkaloid content. The aim of our study was to assay the repellence and toxicity of S. chacoense, its somatic hybrids (SHs) and their...
Article
Full-text available
Heavy metals are dense chemicals with dual biological role as micronutrients and intoxicants. A few hypersaline environmental systems are naturally enriched with heavy metals, while most metal-contaminated sites are a consequence of human activities. Numerous halotolerant and moderately halophilic Bacteria possess metal-tolerance, whereas a few arc...
Article
Dating from the Middle Miocene, the massive halite deposits lying beneath the Transylvanian Basin (Central Romania) have been valuable mineral resources quarried for millennia. Among the numerous hypersaline pit lakes resulted from this mining, Brâncoveanu Lake is unique by its extreme salinity. Assessment of physicochemical variables, water chemis...
Article
Full-text available
Four Erythronium species have been traditionally recognised within Eurasia based on their disjunct distributions and the slight morphological divergence between them: E. dens-canis, E. caucasicum, E. sibiricum and E. japonicum. The range of E. sibiricum includes adjacent parts of southern Siberia, Kazakhstan, China and Mongolia in the Altai-Sayan m...
Article
Full-text available
Hypersaline meromictic lakes are extreme environments in which water stratification is associated with powerful physicochemical gradients and high salt concentrations. Furthermore, their physical stability coupled with vertical water column partitioning makes them important research model systems in microbial niche differentiation and biogeochemica...
Article
Full-text available
Modern mineral deposits play an important role in evolutionary studies by providing clues to the formation of ancient lithified microbial communities. Here we report the presence of microbialite-forming microbial mats in different microenvironments at 32ºC, 49ºC and 65ºC around the geothermal spring from an abandoned oil drill in Ciocaia, Romania....
Article
Full-text available
Perennially stratified salt lakes situated in the Transylvanian Basin (Central Romania) were surveyed for the diversity of culturable halophilic archaea (Fam. Halobacteriaceae). The physical and chemical characteristics of the waters indicated that all the investigated lakes were meromictic and neutral hypersaline. Samples collected from upper, int...
Article
Full-text available
Little attention has been paid so far to the genetic legacy of the oceanic-continental gradient across Europe. Due to this gradient, steppe regions become more extensive and mesic environments become more scattered towards the East. A well-suited system to study the impact of this gradient on lineage differentiation is the temperate mesophilic plan...
Article
Full-text available
Saline, meromictic lakes with significant depth are usually formed as a result of salt mining activity. Ocnei Lake is one of the largest Transylvanian (Central Romania) neutral, hypersaline lake of man-made origin. We aimed to survey the seasonal dynamics of archaeal diversity in the water column of Ocnei Lake by employing microbiological methods a...
Data
Isolation source: saline Ocnei Lake, Romania. Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Isolation source: saline Ocnei Lake, Romania. Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Isolation source: saline Ocnei Lake, Romania. Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Isolation source: saline Ocnei Lake, Romania. Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Isolation source: saline Ocnei Lake, Romania. Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Isolation source: saline Ocnei Lake, Romania. Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Isolation source: saline Ocnei Lake, Romania. Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Isolation source: saline Ocnei Lake, Romania. Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Isolation source: saline Ocnei Lake, Romania. Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Isolation source: saline Ocnei Lake, Romania. Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Data
Sequences were screened for chimeras by the submitter using Bellerophon. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##