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Publications (83)
The bioactive potential of Jeju-Baek-Seohyang (JBS), scientifically known as Daphne jejudoensis M. Kim, was extensively investigated. The antioxidant activity of JBS was relatively lower compared to ascorbic acid in the DPPH and ABTS+ assays. Despite this, JBS exhibited significant anti-inflammatory effects in lipopolysaccharide-stimulated RAW 264....
BACKGROUND/OBJECTIVES
Rosa hybrida has been demonstrated to exert biological effects on several cell types. This study investigated the efficacy of the edible ethanol extract of R. hybrida (EERH) against human colorectal carcinoma cell line (HCT116) cells.
MATERIALS/METHODS
HCT116 cells were cultured with different concentrations of EERH (0, 400,...
Scope
This study evaluates the potential of bioconverted garlic ferments (BGFs) to stimulate the intestinal immune system and modulate cecal microbiota composition.
Methods and results
In vitro, BGF significantly enhances Peyer's patch (PP)‐mediated bone marrow cell proliferation and increases the production of interferon‐gamma (IFN‐γ), granulocyt...
This study evaluated the immunostimulatory potential of garlic fermented with Bacillus subtilis (Aglio) and identified the underlying mechanisms using in vitro and in vivo models.
The aim of this study was to investigate the anti‐inflammatory effects of polysaccharides extracted from crabapples (Malus prunifolia; MP) in vitro and in vivo. Crabapples were subjected to hot water extraction to obtain MP‐HW, which was further separated into low (MP‐L) and high molecular weight (MW) (crude polysaccharide; MP‐CP) fractions by etha...
Previous studies demonstrated the interrelationship between antioxidant activity and solvent extracts prepared according to cultivars and plant parts of Centella asiatica (CA) cultivated in Korea. In this study, the macrophage‐stimulating activity of solvent extracts was assayed, and we analysed the characteristics of the active fractions of these...
Purpose:
The aim of this study was to investigate the efficacy of ethanol extracts of Cornus alba (ECA) against benign prostatic hyperplasia (BPH) in vitro and in vivo.
Materials and methods:
The prostate stromal cells (WPMY-1) and epithelial cells (RWPE-1) were used to examine the action mechanism of ECA in BPH in vitro. ECA efficacy was evalua...
Krill oil (KO) exhibits various biological activities, such as anti-inflammatory and antitumor effects. However, the inhibitory effects of benign prostatic hyperplasia (BPH) in vitro and in vivo have not yet been studied. This study investigated the anti-BPH effects of KO extracted by an enzymatic hydrolysis method. KO treatment inhibited the proli...
Introduction: Recently, nanotechnology has emerged as a potential technique for skin generation, which has several treatment advantages, such as decreased drug cytotoxicity and enhanced skin penetration. Boehmeria tricuspis (BT) belongs to the Urticaceae family and is rich in phenolic and flavonoid compounds. In this study, we biosynthesized gold n...
The present study aimed to investigate the effect of oral administration of snail-derived mucin extract (SM) on ameliorating constipation symptoms of loperamide-induced constipated rats (n = 6). The analytical results indicated that SM mainly contains a glucan-rich snail mucin heteropolysaccharide with high molecular weights (108.5-267.9 kDa), comp...
Interests in the development and exploration of industrial applications of medicinal mushrooms as postbiotics have lately increased. We recently reported the potential use of Phellinus linteus mycelial-containing whole culture extract (PLME) prepared by submerged cultivation as a postbiotic that promotes immune system activation. Here, we aimed to...
Inflammatory skin diseases (ISD) cause very severe itchy skin and dryness which is now a days an important issue which has to be taken care. Nanotechnology plays a main role in manufacturing cosmetic ingredients at a nanoscale size. Among different nanoparticles, gold (Au) is one of the non-toxic materials synthesized organically or inorganically....
Ethnopharmacological relevance
The meadowsweet family (genus Filipendula) includes about 30 species, which have been traditionally used in folk medicine to treat various inflammatory diseases. Particularily, F. palmata (Pall.) Maxim. (Siberian meadowsweet) were traditionally and widely used as an ethnic herb in the Oroqen application.
Aim of the s...
Medicinal mushrooms are an important natural resource promoting health benefits. Herein, Phellinus linteus mycelia were prepared under submerged cultivation, the mycelium-containing culture broth was extracted as a whole to obtain the postbiotic materials (PLME), and its effect on the immune system was evaluated in normal C3H/HeN mice. Oral adminis...
Nanotechnology-applied materials and related therapeutics have gained attention for treating inflammatory skin diseases. The beach rose (Rosa rugosa), belonging to the family Rosaceae, is a perennial, deciduous woody shrub endemic to northeastern Asia. In this study, R. rugosa-based gold nanoparticles (RR-AuNPs) were biologically synthesized under...
Background
Compound K (CK) is the minor ginsenoside present in fermented Panax ginseng extract. Despite the pharmacological efficacy of CK, its industrial use has been restricted due to its low water solubility and poor permeability. To overcome this defect, our study was to synthesize gold nanoparticles from CK (CK-AuNPs) to investigate their pote...
Many countries are endeavoring to strengthen the competitiveness of their biological resources by exploring and developing wild endemic plants. This study examined the effects of Filipendula glaberrima Nakai (FG) on the antioxidant and anti-inflammatory activity using an in vitro system. The bioactive components were also examined using chromatogra...
In this study, we evaluated the effect of oral administration of galacto-oligosaccharide (GOS), famous biological molecules that are comprised of galactose and lactose, on ovalbumin (OVA)-induced allergic dermatitis. OVA-induced mice were divided into the OVA-administered group (OVA-C), promethazine hydrochloride-administered group (PC), and 100 an...
In order to increase the bioavailability of mountain ginseng (MG), gold nanoparticles (MG-AuNPs) were biologically synthesized from MG extract, and an oil-in-water (O/W) nanoemulsion (SMG-AuNEs) was prepared from MG-AuNPs and a phytochemical silydianin. The physical stability of SMG-AuNEs were monitored and optimized in terms of particle size, pH v...
This study was conducted to investigate the effect of the paraprobiotics, lactic acid bacteria lysates (LAB-P) prepared from Lactiplantibacillus plantarum K8, on obesity and obesity-induced inflammatory responses in high-fat diet (HFD)-fed mice. LAB-P (100 mg/kg) significantly decreased the HFD-induced increase in weight by approximately 20% compar...
Background
Despite great advances in the field of immunotherapy, there is still a need for novel and effective immunostimulants to overcome challenges, such as instability and autoinflammatory toxicity, associated with conventional immunostimulants. Nanotechnology provides the possibility to overcome these challenges. The well-known classical Chine...
Paraprobiotics are non-viable cells or cell fractions that have beneficial effects on the host; they may help in overcoming the limitations of probiotics and expanding their benefits. Here, we aimed to investigate their effects on lipid accumulation and the underlying mechanisms in 3T3-L1 preadipocytes. A novel paraprobiotic, LAB-P, prepared from t...
Background: In our previous study, Hibiscus syriacus leaf tissue was successfully cultivated in an optimized callus culture system, and subsequently extracted with 70% ethanol to prepare H. syriacus callus extract (HCE). The previous study suggested that the callus culture is useful method for obtaining the anti-inflammatory ingredients from H. syr...
Centella asiatica L. Urban (CA) is a valuable medicinal herb that contains various bioactive secondary metabolites. In the present study, the harvested CA was divided into whole plant and leaf parts, and were heated-air-dried or freeze-dried. The dried CA was extracted under various extraction conditions to obtain 16 extracts, and their antioxidant...
BACKGROUND
To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO). Hot‐water extracts (DOOS‐HW, 7.5DO‐HW and 9DO‐HW) were prepared from...
BACKGROUND
Persimmon (Diospyros kaki) is a familiar and widespread fruit, cultivated worldwide. To date, physiological and chemical changes in fermented persimmon fruit and its active compounds have been rarely investigated. Moreover, comparative studies on the pharmacological activities of fermented persimmon fruit‐derived compounds have not been...
To investigate the industrial availability of liquid fermentation (PL-ferment) by Phellinus linteus mycelium as a postbiotics for the inhibition of inflammation, PL-ferment was fractionated into culture supernatant (CS), hot-water extract (HW) from PL-ferment, EtOH-precipitate (CP) fractionated from HW, and the dialysate (DCP) of CP. Compared to th...
Animal placentae can be used as health-promoting food ingredients with various therapeutic efficacies, but their use is limited by their unpleasant odor and taste. This study aimed to investigate the possibility of deodorization of sheep placenta via yeast fermentation. A yeast strain was successfully isolated and identified as a novel Brettanomyce...
(1) Background: Nanotechnology is being widely applied for anticancer strategies with few side effects. Nanoparticles (NPs) prepared from natural extracts are promising candidates for cancer treatment because of their unique physicochemical characteristics. This study aimed to prepare gold nanoparticles (AuNPs) from Phyllanthus emblica fruit extrac...
In recent times, the development of functionalized nanoparticle methodology for biomedical applications has become a major challenge. In the present study, we prepared a novel gold nanoparticle (AuNP), named Curto-Cumin AuNP (CC-AuNP), using the biosynthetic process involving Nigella sativa (black cumin) seed extract and membrane vesicles isolated...
Persimmon (Diospyros kaki), a familiar and widespread fruit worldwide, is known to exhibit several physiological effects because of the presence of pharmacologically active compounds called phytochemicals. However, its high-molecular-weight compounds, particularly polysaccharides, have not been extensively studied. In this study, D. kaki extract (D...
Most of the studies on common evening primrose (Oenothera biennis L.) are focused on its oils (isolated from seed, root, and stem tissues). We aimed to investigate the effect of the oral administration of OBS-E on the improvement of skin function in skin-damaged hairless mice exposed to excessive ultraviolet B (UVB) radiation owing to the prelimina...
Although Ginsenosides are a major physiological component of red ginseng (RG), some isolated ginsenosides are known to have cytotoxic activity. This study was intended to reduce cytotoxicity and promote anti-inflammatory activity of ginsenosides by mixing the crude ginsenosides (RG-CG) and polysaccharides (RG-CP). RG-CG-CM, fractionated from RG-CG,...
The present study aimed to investigate the effect of Oenothera biennis sprout extract (OBS-E) on skin-function improvement in an in vitro system and to identify its pharmaceutically active components. OBS-E showed antioxidant ability in radical scavenging and reducing power assays, significantly inhibited matrix metalloproteinases-1 and -2, and inc...
Toward the standardization of common evening primrose (Oenothera biennis) sprout extract (OBS-E), we aimed to obtain indicator compounds and use a validated method. HPLC-UVD allowed simultaneous quantification of the indicator compounds quercetin-3-O-glucuronide and ellagic acid. The method was validated in terms of specificity, linearity, precisio...
Toward the standardization of O. biennis sprout extract (OBS-E), we aimed to obtain indicator compounds, using a validated method. HPLC-UVD allowed simultaneous quantification of indicator compounds quercetin 3-glucuronide and ellagic acid. The method was validated in terms of specificity, linearity, precision, accuracy, and limit of detection/limi...
Paraprobiotics, which are inactivated microbial cells or cell fractions that can confer beneficial effects upon uses, have garnered attention. In this study, we investigated the paraprobiotic effect of Kimchi-derived Lactobacillus plantarum K8 on skin moisturizing activity in human keratinocyte. Four species of lactic acid bacteria were prepared in...
Lemon myrtle leaves were extracted with ethanol at different temperatures (25, 50, and 80 °C) and times (2, 4, 6, and 10 h) to examine the effect of extraction conditions on total polyphenol contents (TPC), total flavonoid contents (TFC), their antioxidant, anti-inflammatory activities, and amount of phenolic compounds. Under optimal extraction con...
Various mixtures were prepared depending on the mixing ratio of Scutellaria baicalensis hot water extract (SB-HW), and Chrysanthemum morifolium ethanol extract (CM-E) and their anti-inflammatory activity were compared. Among them, SB-HW (80 μg/mL)/CM-E (120 μg/mL) or SB-HW (40 μg/mL)/CM-E (160 μg/mL) significantly inhibited LPS-stimulated NO and IL...
Excessive exposure to ultraviolet B (UVB) radiation causes skin aging (a process known as photoaging). This study examined whether subchronic (14-week) oral administration of a novel hydrolyzed chicken sternal cartilage extract—comprising naturally-occurring primarily hydrolyzed type II collagen peptides, chondroitin sulfate and hyaluronic acid, ex...
In this study, we prepared palatinose–sucrose (PS) mixtures from sucrose by enzymatic bioconversion to improve the low sweetness of palatinose and to develop sweeteners that can lower blood sugar levels. We hypothesized that PS mixtures containing 30% or 50% palatinose might demonstrate improvement of hyperglycemia. The physiological changes in C57...
Encapsulated curcumin (ENCC) was prepared from a commercial curcuminoids complex and was evaluated for its intestinal permeability and hepatoprotective effects. Intestinal permeability was evaluated using a Caco-2 intestinal cell monolayer system and the non-everted gut sac method. The hepatoprotective effect was evaluated in experimental rats admi...
Polysaccharide with the enhanced immunostimulatory activities including intestinal immune system modulation was fractionated from Korean red ginseng (KRG) and its characteristics were investigated in the present experiment. When the water extracts were digested with α–amylase and amyloglucosidase and precipitated by ethanol to enhance immunostimula...
Platycodon grandiflorum seeds (PGSE) and P. grandiflorum root extracts (PGRE) were prepared and their effect on glucose uptake were evaluated in C2C12 myotubes. PGSE had significantly higher total phenolic and flavonoid contents than PGRE (P < 0.05), whereas crude saponin contents did not differ significantly between extracts. IC50 values of PGSE a...
The aim of this study was to identify sleep-promoting substance from Polygonatum sibiricum rhizome extract (PSE) with the regulation of sleep architecture. PSE showed a decrease in sleep latency time and an increase in the sleeping time. In the electroencephalography analysis of rats, PSE (150 mg/kg) showed an increase of non-rapid eye movement by...
This study was conducted to convert palatinose from sugar by biotransformation, and to suggest novel methods for characterizing palatinose. When 30 brix solution of sucrose was incubated with 1% α-glycosyltransferase for 15 h at 30ºC, conversion of sucrose into the palatinose was more effective than under other conditions. In this study, 0.5% α-gly...
Barley has nutritional benefits due to its high dietary fiber content; therefore, the intake of whole barley grains is recommended. However, barley is often consumed in the fermented form because of the improved texture and digestibility. The present study was designed to elucidate the intracellular signaling pathway for macrophage activation by th...
In this study, we developed a novel organic galactooligosaccharide (GOS) by using β-galactosidase from organic lactose to perform transgalactosylation, and the quality of the novel organic GOS was compared to that of commercial GOS. A new β-galactosidase isolated from Bacillus species converted 80% of lactose composed of galactose and glucose into...
Six bacterial strains isolated from various Korean fermented foods were cultured in cellobiose-containing medium to investigate their potential for producing new kind of oligosaccharides. After bacterial culture in a liquid medium, each culture medium was concentrated and analyzed. TLC analysis revealed that only one strain (Bacillus subtilis SS-76...
Four polysaccharide fractions were prepared from barley leaves and then in vitro intestinal immunostimulatory activity was compared. Among fractions, high molecular weight fraction prepared by enzyme extraction, namely BLE-P, showed the potent in activating bone marrow cell proliferation through Peyer’s patch (PP), and in stimulating cytokine produ...
To obtain the immunomodulating polysaccharide from chaga mushroom (Inonotus obliquus sclerotia, IO), crude polysac- charide fractions (IO-M-CP and IO-CP, respectively) prepared from hot-water extract (IO-W) of I. obliquus by EtOH precipitation after MeOH reflux or not. After IO-W was re-dissolved in water followed by EtOH addition in the case witho...
In this study, diverse intestinal immunostimulatory activities were demonstrated for polysaccharides (KBV-CP) isolated from Korean brown rice vinegar. Monosaccharide composition analysis indicated that KBV-CP was composed mainly of neutral sugar units, primarily glucose and mannose. In vitro, KBV-CP significantly augmented the productions of immuno...
To enhance the physiological activities of roasted coffee (RC), 30 kinds of green coffee beans (GCB) with different cultivating areas and varieties were fermented with Monascus ruber mycelium (MR) by solid-state culture. After the dried MR-fermented GCB was subjected medium roasting, each RC was extracted with hot-water. Among the hot-water extract...
Korean fresh ginseng was cultured with Hericium erinaceum mycelia (HE) in solid-state culture (SSC) to enhance the immunomodulation activity. Hot-water extracts (FG-HE-HW) of H. erinaceum-fermented ginseng (FG-HE) fractionated into a crude polysaccharide (FG-HE-CP) showed higher mitogenic, macrophage stimulation, and intestinal immune system modula...
The in vitro and in vivo effects of crude polysaccharide isolated from Korean persimmon vinegar (KPV-0) on the intestinal immunostimulation were examined. KPV-0 was not cytotoxic to intestinal epithelial Caco-2 cells and could be transported through a Caco-2 cell monolayer in an in vitro coculture system. For and in vitro experiment, KPV-0 treatmen...
Obesity is the leading metabolic disease in industrialized countries and is closely associated with coronary heart disease, hypertension, diabetes, and cancer. The objective of this study was to evaluate the anti-adipogenic effects of two roasted coffee beans, Vietnam robusta (VR) and Ethiopia Mocha Sidamo G2 (ES), as well as fermented coffee beans...
We investigated the anti-obesity and anti-hyperlipidemic effects of extracts of two roasted coffee beans (Vietnam robusta and Ethiopia mocha sidamo G2) and fermented coffee beans with Monascus rubber mycelium (MR) by solid-state culture. C57B/L6 mice were divided into seven groups: normal diet (ND) group, high fat diet (HFD) group, and HFD groups w...
In previous work, we fermented coffee beans using solid-state culture with various fungal mycelia to enhance the physiological activity of the coffee. The coffee fermented with Monascus sp. showed a higher physiological activity than non-fermented coffee or other coffees fermented with mushroom mycelium. The aim of this study was to characterize th...
Abstract Fresh ginseng was fermented with Ganoderma lucidum mycelium (GL) in solid-state culture to enhance its immunomodulatory activity. When crude polysaccharide (FG-GL-CP) was fractionated from lyophilized fermented ginseng (FG-GL), FG-GL-CP showed significantly higher mitogenic, macrophage stimulating, and intestinal immune system modulating a...
Green coffee beans (CB, Indonesian Mandheling) were fermented with three kinds of mushrooms (Phellinus linteus, PL; Hericium erinaceum, HE; Ganoderma lucidum, GL) or two kinds of mycelia from molds (Monascus purpureus, MP; Monascus ruber, MR) using solid-state culture to enhance physiological activity. After the roasting of fermented green coffee b...
Much interest has been generated for biotechnological methods in the production of polysaccharides that can be applied within the food, pharmaceutical, cosmetic, and other industries. The majority of polysaccharides with various physiological activities are frequently derived from fungi, especially mushrooms (Yu et al. 2005; Yoon et al. 2008). Some...
To investigate the pharmacological activity of chaga mushroom (Inonotus obliquus) on extraction conditions, chaga was extracted using water (reflux at , decoction over , pressure at ) or ethanol (reflux at 50, 70, or ). When water extract was further fractionated into crude polysaccharide (IO-CP), yields of IO-CP (4.8~16.8%) were higher than those...
After Ganoderma lucidum was cultured in mushroom complete medium (MCM) supplemented with ginseng extract (GE), crude polysaccharide (GL-GE-CP) was fractionated from mycelium. Among GL-GE-CP from mycelium in MCM supplemented with 5, 10, and 15% GE (v/v ratio of MCM to GE), GL-GE-15-CP (15% GE) most significantly enhanced macrophage stimulation and i...
After mulberry (Morus alba) leaves were fermented with Hericium erinaceum mycelium by solid-state culture to enhance physiological activity, fermented mulberry leaves (MA-HE) was extracted by hot-water (MA-HEHW) and ethanol (MA-HE-E). MA-HE-HW showed enhanced mitogenic and intestinal immune system modulating activities (1.41 and 1.52 fold of saline...
Crude polysaccharide (CP) was fractionated from the submerged culture (containing both mycelia and culture broth, SC) with Phellinus linteus (PL) in mushroom complete medium (MCM) supplemented with ginseng extract (65°Bx, GE) to enhance the immune activity. PL-GE-15-CP from SC cultivated in MCM supplemented with GE-15% (v/v, a ratio of MCM volume t...
After bearded tooth mushroom (Hericium erinaceum) mycelia were cultivated in mushroom complete medium (MCM) supplemented with ginseng extract (GE, 65°Bx) to enhance immunostimulation
and anti-metastasis, the submerged culture containing culture broth and mycelia was lyophilized (SC). Hot-water extract from
SC cultivated in MCM supplemented with GE-...
Crude polysaccharide (WG-PL-CP) was fractionated from fermented ginseng with Phellinus linteus in solid culture to enhance the immunostimulation of ginseng. WG-PL-CP produce three active polysaccharide-rich fractions (WG-PL-CP-II, III, and IV) on DEAE-Sepharose CL-6B (Cl- form). WG-PL-CP-III displayed higher mitogenic activity (1.98- fold of the sa...
To find the new use of Korean ginseng and mushroom, crude polysaccharides were prepared from submerged cultures of Hericium erinaceum in the medium supplemented with Korean ginseng extracts. When we fractionated crude polysaccharides (HE-GE-CP-1, 3, and 5) from hot-water extracts of submerged cultures of H. erinaceum with ginseng extracts (1%, 3%,...
For the utilization of Korean ginseng (Panax ginseng C.A. Meyer) in the functional drink, we prepared the fermented Korean ginseng with mushroom mycelia (Ganoderma lucidum; WG-GL, Hericium erinaceum; WG-HE and Phellinus linteus; WG-PL) by solid culture. A proximate analysis showed that the fermented Korean ginseng contained significantly more crude...
