Hongwen Huang

Chinese Academy of Sciences | CAS · Lushan Botanical Garden

High temperatures have a significant impact on plant growth and metabolism. In recent years, the fruit industry has faced a serious threat due to high-temperature stress on fruit plants caused by global warming. In the present study, we explored the molecular regulatory mechanisms that contribute to high-temperature tolerance in kiwifruit. A total...

Background The base editors can introduce point mutations accurately without causing double-stranded DNA breaks or requiring donor DNA templates. Previously, cytosine base editors (CBEs) containing different deaminases are reported for precise and accurate base editing in plants. However, the knowledge of CBEs in polyploid plants is inadequate and...

Background: Human-guided crop domestication has lasted for more than 10,000 years. In terms of the domestication and breeding of vegetables, cellulose content in edible tissues is one of the most important traits. Primulina eburnea is a recently developed calcium-rich vegetable with a high soluble and bioavailable calcium content in its leaves. Ho...

Introduction Flower color is an ideal trait for studying the molecular basis for phenotypic variations in natural populations of species. Epimedium (Berberidaceae) species exhibit a wide range of flower colors resulting from the varied accumulation of anthocyanins and other pigments in their spur-like petals and petaloid sepals. Methods In this wo...

The basal eudicot Epimedium (Barrenwort) exhibits innovative floral morphology in the form of petal spurs filled with nectar and petaloid sepals. The B-class MADS-box genes APETALAS3 (AP3) and PISTILLA (PI) determine sepal and petal identity while the C-class gene AGAMOUS (AG) determines carpel and stamen identity in Arabidopsis. Complex histories...

The STAY-GREEN (SGR) proteins play an important role in chlorophyll (Chl) degradation and are closely related to plant photosynthesis. However, the availability of inadequate studies on SGR motivated us to conduct a comprehensive study on the identification and functional dissection of SGR superfamily members in kiwifruit. Here, we identified five...

Background: The base editors can introduce point mutations accurately without causing double-stranded DNA breaks or requiring donor DNA templates. Previously, cytosine base editors (CBEs) containing different deaminases are reported for precise and accurate base editing in plants. However, the knowledge of CBEs in polyploid plants is inadequate and...

The current kiwifruit industry is mainly based on the cultivars derived from the species Actinidia chinensis (Ac) which may bring risks such as canker disease. Introgression of desired traits from wild relatives is an important method for improving kiwifruit cultivars. Actinidia eriantha (Ae) is a particularly important taxon used for hybridization...

Akebia species, belonging to Lardizabalaceae, are widespread from subtropical to temperate environments of China, Japan, and Korea. All known Akebia species have medicinal and dietary value and have been widely cultivated as a new fruit crop in many areas of China. However, compared with other crop species, the breeding improvement and commercial c...

Genus Actinidia, Kiwifruit

The Genus Actinidia Or THE GENUS ACTINIDIA, A World Monograph Forward Preface Acknowledgment INTRODUCTION CHAPTER ONE SYSTEMATICS AND GENETIC VARIATION OF ACTINIDIA 1 Taxonomic Revision 1.1 Actinidia 1.2 Clematoclethra 1.3 Saurauia 2 Taxa Treatments 2.1 Varieties in A. arguta (Sieb. & Zucc.) Planch. ex Miq. 2.2 Varieties in A. callosa Lindley 2...

The exploration and use of wild plant resources goes back to our rooted history of human civilization over about 20,000 years ago, before Ancient Mesopotamia in the Valley of the Tigris and Euphrates where barley, lentil and wheat were first domesticated [...]

Light-harvesting chlorophyll a/b-binding (LHC) protein is a superfamily that plays a vital role in photosynthesis. However, the reported knowledge of LHCs in kiwifruit is inadequate and poorly understood. In this study, we identified 42 and 45 LHC genes in Actinidia chinensis (Ac) and A. eriantha (Ae) genomes. Phylogenetic analysis showed that the...

Elaeagnus L. is found in wild or grown as ornamental plants and is increasingly regarded as underutilized berry shrubs by breeders. This genus has cosmopolitan distribution with various species widely distributed in China, Europe, the United States, and Canada. Interspecific hybrids, which have been reported several times, have attracted intense in...

Akebia trifoliata is a high-value medicinal and edible fruit crop in China, and it has begun to be widely cultivated as a new fruit crop in many areas of China. Its fruits crack longitudinally when fully ripe and should be harvested before fruit cracking. Physicochemical characteristics and nutritional composition of the ripening process are prereq...

Key message Domestication traits particularly fruit size and plant architecture and flowering are critical in transforming a progenitor’s wild stature into a super improved plant. The latest advancements in the CRISPR system, as well as its rapid adoption, are speeding up plant breeding. Abstract Solanaceae has a varied range of important crops, w...

A neutronic experiment has been performed using DT neutrons on a blanket mockup of the hybrid tritium breeding blanket (HTBB) at CAEP. As a backup blanket configuration for CFETR, HTBB design which uses uranium alloy, water coolant and ceramic breeder pebble bed has been proposed to obtain high tritium breeding ratio. The experiment has provided va...

Background Goji ( Lycium spp., 2n = 24) is a fruit bearing woody plant popular as a superfood for extensive medicinal and nutritional advantages. Fruit size associated attributes are important for evaluating small-fruited goji berry and plant architecture. The domestication traits are regulated quantitatively in crop plants but few studies have att...

Lists of my books and chapters

Background: Goji (Lycium spp., 2n = 24) is a perennial woody plant bearing functional properties. Fruit size associated attributes are important for evaluating small-fruited goji berry and plant architecture. The domestication traits are regulated quantitatively in crop plants but fewer studies have attempted on genomic regions corresponding to the...

Background: Goji (Lycium spp., 2n = 24) is a fruit bearing woody plant popular as a superfood for extensive medicinal and nutritional advantages. Fruit size associated attributes are important for evaluating small-fruited goji berry and plant architecture. The domestication traits are regulated quantitatively in crop plants but few studies have att...

Background: Goji (Lycium spp., 2n = 24) is a fruit bearing woody plant popular as a superfood for extensive medicinal and nutritional advantages. Fruit size associated attributes are important for evaluating small-fruited goji berry and plant architecture. The domestication traits are regulated quantitatively in crop plants but few studies have att...

Stauntonia obovatifoliola Hayata subsp. urophylla is perennial, evergreen vine producing large edible fruits commonly known as “Jiuyuehuang” (September yellow) in China. In fact, rich nutritional value and favorite taste of S. obovatifoliola Hayata subsp. urophylla give it new potential as a new edible fruit crop. Investigation on genetic diversity...

A simple and effective protocol was established for recurrent somatic embryogenesis and plant regeneration in Akebia trifoliata (Thunb.) Koidz. Somatic embryos directly formed from the root zone of the immature zygotic embryos cultured on MS medium devoid of plant growth regulator (PGR). The induction frequency of immature zygotic embryos was 52.5%...

Background The Chinese chestnut (Castanea mollissima) is widely cultivated in China for nut production. This plant also plays an important ecological role in afforestation and ecosystem services. To facilitate and expand the use of C. mollissima for breeding and its genetic improvement, we report here the whole-genome sequence of C. mollissima. Fi...

Angiosperm mitochondrial genomes (mitogenomes) are notable for their extreme diversity in both size and structure. However, our current understanding of this diversity is limited, and the underlying mechanism contributing to this diversity remains unclear. Here, we completely assembled and compared the mitogenomes of three kiwifruit (Actinidia) spe...

Diversity in structure and organization is one of the main features of angiosperm mitochondrial genomes (mitogenomes). The ultra-long reads of Oxford Nanopore Technology (ONT) provide an opportunity to obtain a complete mitogenome and investigate the structural variation in unprecedented detail. In this study, we compared mitogenome assembly method...

Akebia trifoliata is perennial and deciduous vines, its fruit, seed, stem and root have been used in Chinese herbalism for at least 2000 years. As a high-value medicinal, the A. trifoliata should be exploited and domestication is underway as edible fruit crop. In the present study, a wild seedlings population and a three-generation selected populat...

Premise of the Study Euchresta horsfieldii (Fabaceae) is a rare and endangered medicinal plant in Indonesia with restricted distribution. Single‐copy nuclear DNA (scnDNA) markers were developed for this species to facilitate further investigation of genetic diversity and population structure. Methods and Results We performed RNA‐Seq and de novo as...

Figure S1 Overall structures of vectors used in the CRISPR/Cas or PTG/Cas9 systems. Figure S2 PCR validation and Sanger sequencing of PCR products amplified from paired‐sgRNA/Cas9 vectors. Figure S3 Validation of transgene‐positive cells in G418‐resistant callus lines using Cas9‐specific primers SP‐DL/SP‐R. Figure S4 Relative expression of sgRNA...

Akebia trifoliata belongs to the Lardizabalaceae family of flowering plants, has tremendous potential as a new fruit crop for further domestication and commercialization in China. However, the selection of A. trifoliata is extremely limited due to lack of information about basic inheritance of fruit traits. In this study, 11 key fruit characters, i...

A new general nuclide inventory analysis code named GNIAC has been developed under the motivation of developing a modern and general-purpose tool to simulate nuclide transmutation process for nuclear applications. GNIAC applies both the Partial Fraction Decomposition (PFD) and Incomplete Partial Fraction (IPF) forms of Chebyshev Rational Approximat...

Background: Most published genome sequences are drafts, and most are dominated by computational gene prediction. Draft genomes typically incorporate considerable sequence data that are not assigned to chromosomes, and predicted genes without quality confidence measures. The current Actinidia chinensis (kiwifruit) 'Hongyang' draft genome has 164 Mb...

The advance trajectory of scientific research in the world botanical gardens over past 500 years is not only a microcosm of the whole history of biological sciences, but also reveal the unremitting pursuit of mankind exploration and utilization of plant resources for development of economy and society. From the 16th-17th centuries, botanical garden...

Interactions between commensal microbes and invading pathogens are understudied, despite their likely effects on pathogen population structure and infection processes. We describe the population structure and genetic diversity of a broad range of co-occurring Pseudomonas syringae isolated from infected and uninfected kiwifruit during an outbreak of...

Interactions between commensal microbes and invading pathogens are understudied, despite their likely effects on pathogen population structure and infection processes. We describe the population structure and genetic diversity of a broad range of co-occurring Pseudomonas syringae isolated from infected and uninfected kiwifruit during an outbreak of...

Kiwifruit is an important fruit crop; however, technologies for its functional genomic and molecular improvement are limited. The clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system has been successfully applied to genetic improvement in many crops, but its editing capability is variable depend...

In radiation shielding problem as well as nuclear reactor analysis applications, Monte Carlo (MC) method is considered as the most useful tool for its obvious advantage to deal with heterogeneous and complex system model. However, for the deep-penetration problem in shielding calculation, analog MC method is inefficient since particles have little...

The role of living collections-based research and discovery has been a prominent feature throughout the history of evolution and advance of botanical science: such research is the core and soul of the botanical gardens. Currently, there are c. 162 Chinese botanical gardens, harboring c. 20,000 species in China. As an example of initiatives to utili...

Analysis of the John Bradby Blake plant illustrations in the library of the Oak Spring Garden Foundation identifies a total of 138 species belonging to over 100 genera and 62 families. Especially well-represented are useful plants of all kinds, including a high proportion of plants that are used in Chinese traditional medicine.

A preliminary design of fusion–fission hybrid energy reactor (FFHER) has been proposed by Institute of Nuclear Physics and Chemistry based on current fusion science and well-developed fission technology. In FFHER, shield blocks provide nuclear shielding and thermal shielding for internal and external blanket components. The hybrid of fusion core an...

Monte Carlo (MC) method is extensively employed in shielding calculations with the advantages of high fidelity geometry modeling, complex radiation source description and continuous-energy cross sections. In global response problem, the detailed response estimates are required for the entire phase space. However, in deep-penetration shielding simul...

The modern botanical gardens that originated in mid-16th century with the rise of natural sciences and gardening arts during the late European renaissance, reached its heyday during the 18th century when Western colonizers explored, studied, collected, and spread plants all over the world. The 500-year history of botanical gardens is full of human...

An outbreak of kiwifruit bacterial canker disease caused by Pseudomonas syringae pv. actinidiae (Psa) beginning in 2008 caused disaster to the kiwifruit industry. However the mechanisms of interaction between kiwifruit and Psa are unknown. Long noncoding RNAs (lncRNAs) are known to regulate many biological processes, but comprehensive repertoires o...

Reticulate speciation caused by interspecific hybridization is now recognized as an important mechanism in the creation of biological diversity. However, depicting the patterns of phylogenetic networks for lineages that have undergone interspecific gene flow is challenging. Here we sequenced 25 taxa representing natural diversity in the genus Actin...

Complex mechanics and packing instability can be induced by loading operation on ceramic breeder pebble bed for its discrete nature. A numerical approach using discrete element method (DEM) is applied to study the mechanical performance of the ceramic breeder pebble bed under quasi-static and cyclic loads. A preloaded specimen can be made with serv...

Research studies have recently focused on circle RNAs (circRNAs) in relation to their regulatory functions in animals. However, the systematic identification of circRNAs in plants, especially non-model plants, is limited. In addition, raw report on the prediction of the potential role of circRNAs in plant response to pathogen invasion is currently...

Recurring epidemics of kiwifruit (Actinidia spp.) bleeding canker disease are caused by Pseudomonas syringae pv. actinidiae (Psa). In order to strengthen understanding of population structure, phylogeography and evolutionary dynamics, we isolated Pseudomonas from cultivated and wild kiwifruit across six provinces in China. Based on the analysis of...