Hong Zhan

Hong Zhan
The Discovery Building · Department of Virology

PhD

About

14
Publications
4,209
Reads
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341
Citations
Citations since 2016
6 Research Items
289 Citations
201620172018201920202021202201020304050
201620172018201920202021202201020304050
201620172018201920202021202201020304050
201620172018201920202021202201020304050
Additional affiliations
May 2018 - present
The Discovery Building
Position
  • Fellow
December 2014 - April 2018
University of Wisconsin–Madison
Position
  • PostDoc Position
February 2014 - May 2014
University of Southern California
Position
  • Visitor scholar
Description
  • In vivo single molecule tracking voltage-dependent calcium channels in live C. elegans
Education
September 2010 - October 2014
Sorbonne Université
Field of study
  • Neurobiology and Biophysics
September 2008 - August 2010
Université de Technologie de Compiègne
Field of study
  • Biotechnology
September 2005 - October 2010
Shanghai University of Engineering Science
Field of study
  • Bio-engineering

Publications

Publications (14)
Article
Full-text available
The active zone (AZ) of chemical synapses is a specialized area of the presynaptic bouton in which vesicles fuse with the plasma membrane and release neurotransmitters. Efficient signaling requires synaptic vesicles (SVs) to be recruited, primed, and retained at the AZ, in close proximity to voltage-dependent calcium channels that are activated dur...
Article
Because most neurons receive thousands of synaptic inputs, the neuronal membrane is a mosaic of specialized microdomains where neurotransmitter receptors cluster in register with the corresponding presynaptic neurotransmitter release sites. In many cases the coordinated differentiation of presynaptic and postsynaptic domains implicates trans-synapt...
Article
Full-text available
The strength of synaptic connections varies significantly and is a key determinant of communication within neural circuits. Mechanistic insight into presynaptic factors that establish and modulate neurotransmitter release properties is crucial to understanding synapse strength, circuit function, and neural plasticity. We previously identified Droso...
Article
Full-text available
Single-molecule (SM) fluorescence microscopy allows the imaging of biomolecules in cultured cells with a precision of a few nanometres but has yet to be implemented in living adult animals. Here we used split-GFP (green fluorescent protein) fusions and complementation-activated light microscopy (CALM) for subresolution imaging of individual membran...
Article
Full-text available
Significance Positive-strand RNA [(+)RNA] viruses constitute the largest genetic class of viruses and include many high-impact pathogens, such as SARS–CoV-2 (COVID-19 pandemic coronavirus), MERS CoV, Zika, chikungunya, dengue, and hepatitis C viruses. (+)RNA virus genome replication invariably occurs on virus-induced, membrane-bound organelles call...
Article
Full-text available
Positive-strand RNA virus RNA genome replication occurs in membrane-associated RNA replication complexes (RCs). Nodavirus RCs are outer mitochondrial membrane invaginations whose necked openings to the cytosol are “crowned” by a 12-fold symmetrical proteinaceous ring that functions as the main engine of RNA replication. Similar protein crowns recen...
Article
Full-text available
The nodavirus flock house virus recently provided a well-characterized model for the first cryo-electron microscope tomography of membrane-bound, positive-strand RNA ((+)RNA) virus genome replication complexes (RCs). The resulting first views of RC organization and complementary biochemical results showed that the viral RNA replication vesicle is t...
Article
Precise Protein Localization in Dissected Drosophila Larvae by Correlative Light and Transmission Electron Microscopy - Volume 24 Supplement - Hong Zhan, Elle Kielar-Grevstad, Kate O'Connor-Giles
Article
Full-text available
We combined cryo-preservation of intact Drosophila larvae and electron tomography with comprehensive segmentation of key features to reconstruct the complete ultrastructure of a model glutamatergic synapse in a near-to-native state. Presynaptically, we detail a complex network of filaments that connects and organizes synaptic vesicles. We link the...
Article
Full-text available
Synapses are the fundamental functional units of neural circuits, and their dysregulation has been implicated in diverse neurological disorders. At presynaptic terminals, neurotransmitter-filled synaptic vesicles are released in response to calcium influx through voltage-gated calcium channels activated by the arrival of an action potential. Decade...
Article
We propose a structured illumination microscopy method to combine super resolution and optical sectioning in three-dimensional (3D) samples that allows the use of two-dimensional (2D) data processing. Indeed, obtaining super-resolution images of thick samples is a difficult task if low spatial frequencies are present in the in-focus section of the...
Article
Several human diseases, including hypokalemic periodic paralysis and Timothy syndrome, are caused by mutations in voltage-gated calcium channels. The effects of these mutations are not always well understood, partially because of difficulties in expressing these channels in heterologous systems. The use of C. elegans could be an alternative approac...
Article
Full-text available
Single-molecule (SM) fluorescence microscopy allows the imaging of biomolecules in cultured cells with a precision of a few nanometres but has yet to be implemented in living adult animals. Here we used split-GFP (green fluorescent protein) fusions and complementation-activated light microscopy (CALM) for subresolution imaging of individual membran...

Questions

Questions (5)
Question
Dear all,
I recently resolved a protein atomic structure by CryoEM. And intriguingly, the protein's structure shows similarity to a group of metatransferase (MTase) that on the PDB database, however, the when I looked purely sequence alignment to these MTase proteins, the conservation is pretty poor. I would like to know, whether there is an algorithm that can generate Phylogenetic metrics using structural alignment?
Many thanks!
Best,
Hong
Question
Dear all,
I am doing a High pressure freezing on S2 cell culture. I recently got nice picture. I do not have ice damaging in nucleus and I can see well double layer lipid membrane. However, there is one feature puzzled me as I showed in this attached pic, where I segmented this vesicle in white line. Is there anyone know what kind of potential organelles could be? My first through is smooth ER... but it is way too big as my experience... lysome? The lipid droplette is in the same pic too, and it is big white vesicle...
Thanks in advance for any inputs!
Best,
Hong
Question
Is there any easy way to do a 2D Gaussian fitting of image (array) in R? 
Question
As in the title...a membrane associated protein fused with GFP (target protein is expressed in nervous system). I guess signal peptide is not functioning as normal.. homozygous of GFP tagged protein is lethal for flies (stop development before hatch). But in embryo, GFP expression seems like in nervous system. Have to further to see whether they go to the membrane. Anyone has some comments on that? 
Question
Is it any literature about optogenetic (channelrhodopsin/halorhodopsin) and SPT in cultured cell or tissues?

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Projects

Projects (3)
Project
By using Cryo-electron tomography and sub-tomogram averaging, I am investigating the molecular mechanism of viral RNA replication of (+)-strand RNA virus at atomic resolution.
Archived project
To decipher molecular mechanism of presynaptic cytomatrix that regulates synaptic transmission