Holger Christiansen

Holger Christiansen
University of Leipzig · Department of Pediatric Oncology, Hematology and Blood Coagulation

Univ.-Prof. Dr. med.

About

144
Publications
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4,689
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Additional affiliations
December 2008 - present
University of Leipzig
October 1996 - November 2008
Philipps University of Marburg
May 1984 - September 1996
Justus-Liebig-Universität Gießen

Publications

Publications (144)
Article
Full-text available
Nasopharyngeal carcinoma (NPC) in children and young adults has been treated within two consecutive prospective trials in Germany, the NPC-91 and the NPC-2003 study of the German Society of Pediatric Oncology and Hematology (GPOH). In these studies, multimodal treatment with induction chemotherapy, followed by radio (chemo)therapy and interferon-be...
Article
Full-text available
Objective: Recent evidence suggests that illness perceptions in paediatric patients and their parents may differ, with parents holding more negative views compared to their children. Little is known about illness perceptions of very young patients and their parents. This study investigates illness perceptions in paediatric cancer patients aged 4-1...
Article
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Background: To identify variables predicting outcome in neuroblastoma patients assigned to the high-risk group solely by the presence of MYCN oncogene amplification (MNA). Methods: Clinical characteristics, genomic information, and outcome of 190 patients solely assigned to high-risk neuroblastoma by MNA were analyzed and compared to 205 patient...
Article
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Aim In 2015, the revised International Pediatric Non-Hodgkin Lymphoma Staging System was published. It mentions [¹⁸F]-FDG-PET/MRI as the latest method to perform whole-body imaging. However, supporting data are pending. Our aim was to investigate the performance of whole-body [¹⁸F]-FDG-PET/MRI in pediatric non-Hodgkin lymphoma patients by using a l...
Article
We recently identified Galectin-1 (Gal-1), a β-galactoside-binding lectin, as a novel immune regulator in neuroblastoma (NB). Here, we characterized the tolerogenic function of Gal-1 within the CD8+ T cell compartment and further evaluated its relevance as an antigen for effective DNA vaccination against NB in a mouse model. NB cells with Gal-1 kno...
Article
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Introduction The survival of children with stage 4(M) neuroblastoma without MYCN amplification and below the age of 18 months is considered better than the still dismal outcome of older high‐risk neuroblastoma patients. This study analyzes the impact of clinical and molecular characteristics on the long‐term outcome. Patients and methods Clinical...
Chapter
Im Kindesalter sind primäre Tumoren des Respirationstrakts sehr selten. Die meisten Neoplasien des Lungenparenchyms sind Metastasen. In die Lunge metastasierende Tumoren bei Kindern sind: der Wilms-Tumor, das Osteosarkom, Ewing-Sarkom, Rhabdomyosarkom und Hepatoblastom.
Article
Background Recent research shows that parents of children suffer from fear of progression (FoP), the fear of further disease progression. It is most possible that children also develop FoP, which could impair treatment and psychological health. The aim of this study is to adapt the adult’s version of the Fear of Progression Questionnaire – Short Fo...
Article
We conducted a clinical trial and report the long-term outcome of 773 children with acute lymphoblastic leukemia upon risk-adapted therapy accrued in trial CoALL 07-03 (from the Cooperative Study Group for Childhood Acute Lymphoblastic Leukemia). In a 2-step stratification, patients were allocated to receive either low- or high-risk treatment, base...
Article
Background HD is a B-lineage lymphoma characterized, depending on subtype, by a prominent inflammatory infiltrate and fibrosis. Clinically, inflammatory symptoms like fever, weight loss and night sweats (B-symptoms) and increased blood biomarkers of inflammation, including ESR and CRP, are characteristic of more advanced disease. The clinical trial...
Article
This study investigated the association of individual and dyadic coping strategies with fear of progression (FoP) in mothers and fathers of children with hematologic cancer. Parental couples (N = 44) whose children had been diagnosed with hematologic cancer were recruited at a university hospital and a rehabilitation clinic in Germany between 03/20...
Article
Belastende medizinische Maßnahmen wie bei einer Krebserkrankung führen bei Kindern und Jugendlichen zunehmend zu psychischen und psychosozialen Langzeitfolgen. Eine individuell angepasste psychologische Vorbereitung kann dabei helfen, den Patienten zu stabilisieren und Angst zu reduzieren.
Article
Background Potentially life-threatening pediatric illnesses as well as life-limiting disabilities and their treatment imply long-term psychosocial consequences for the whole family. Objective From the perspective of healthy siblings in adolescence and adulthood, the study explores psychosocial effects of (ongoing) illness experience or loss experi...
Article
Zusammenfassung Regelmäßige körperliche Aktivität ist eine wichtige Voraussetzung für die Gesundheit und die Entwicklung von Kindern und Jugendlichen. Insbesondere während aber auch nach einer Krebserkrankung sind Kinder und Jugendliche jedoch in sehr geringem Umfang körperlich aktiv und weisen eine stark ausgeprägte körperliche Inaktivität auf. Di...
Article
Anthracyclines are integral components of antileukemic treatment. Apart from cardiotoxicity, myelosuppression and infectious complications have been described for doxorubicin (DOX) and daunorubicin (DNR) as predominant side effects, but little is known about their differential toxicities. To address the question whether DNR is associated with a low...
Article
Background: Fear of Progression (FoP) is a commonly reported psychological strain in parents of children with cancer. This expert survey investigates how professionals in pediatric oncology estimate the burden and consequences of FoP in parents and how they assess and treat parental FoP. Method: N=77 professionals in pediatric oncology (members an...
Article
Objective Psychometric properties of the Fear of Progression Questionnaire – Short Form (FoP-Q-SF) were shown to be good in samples of adult cancer patients and their partners, but have so far not been investigated in parents of children with cancer. This study therefore aimed to examine psychometric properties of the previously adapted parent vers...
Article
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We determined the indication, outcome, and risk factors of single and multiple hematopoietic stem cell transplantation(s) (HSCT) in children and adolescents mostly with advanced disease. Forty-one out of 483 patients (8.5 %; median age 9 years) diagnosed at the University of Leipzig with hematological and oncological diseases required HSCT from 199...
Article
Heme oxygenase (HO)-1 catalyzes the degradation of cytotoxic heme into biliverdin and blocks anti-tumor immune responses, thus protecting cancer against host defense. Whether this scenario also applies to neuroblastoma (NB), the most common extra-cranial solid childhood tumor, is not known. Here, we demonstrate for the first time a prognostic relev...
Article
Objectives To assess knowledge deficits of patients/parents and prevention strategies. Methods After receiving ethics approval, we performed a controlled, quasi-randomised, prospective intervention study. We enrolled patients/parents involved in managing oral medicines in three groups: control (routine care only), handbook intervention and pharmace...
Article
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In pediatric patients, brain tumors have been estimated to be the cause for seizures in only 0.2–0.3 % of cases, whereas seizures occurred in about 13 % of pediatric brain tumor patients at presentation. This survey was conducted to analyze EEG findings in pediatric tumor patients over the past 14 years to evaluate the diagnostic value of preoperat...
Article
Full-text available
Background: Fear of Progression (FoP), the fear of further disease progression, is one of the most common psychological strains of chronically ill patients and can also be found in healthy partners of cancer patients. Parents of children with cancer are also at risk of developing distinct fears that may persist after medical treatment. This study a...
Article
Full-text available
Pediatric tumors of the central nervous system composed of oligoid tumor cells showing diffuse leptomeningeal spread without a primary mass lesion seem to represent a novel tumor entity. The terms “diffuse leptomeningeal glioneural tumor” or—preferably—“disseminated oligodendroglial-like leptomeningeal tumor of childhood” (DOGLT) were proposed. Fou...
Chapter
Im Kindesalter sind primäre Tumoren des Respirationstrakts sehr selten. Die meisten Neoplasien des Lungenparenchyms sind Metastasen. In die Lunge metastasierende Tumoren bei Kindern sind: der Wilms-Tumor, das Osteosarkom, Ewing-Sarkom, Rhabdomyosarkom und Hepatoblastom.
Article
Full-text available
Die PET/MRT ist die neueste und innovativste Entwicklung auf dem Gebiet der bildgebenden Diagnostik. Die wesentlich geringere Strahlenexposition im Vergleich zur PET/CT stellt gerade fur kinderonkologische Fragestellungen einen ganz entscheidenden Vorteil dar. Die gleichzeitige Abbildung von Metabolismus, verschiedenen funktionellen Parametern und...
Chapter
Im Kindesalter sind primäre Tumoren des Respirationstrakts sehr selten. Die meisten Neoplasien des Lungenparenchyms sind Metastasen. In die Lunge metastasierende Tumoren bei Kindern sind: der Wilms-Tumor, das Osteosarkom, Ewing-Sarkom, Rhabdomyosarkom und Hepatoblastom. Die primären intrathorakalen Neubildungen befinden sich am häufigsten im Medias...
Article
Full-text available
An integrated PET/MRI scanner has been used in selected cases of pediatric brain tumor patients to obtain additional metabolic information about lesions for preoperative biopsy planning and navigation. Four patients, age 9-16 years, received PET/MRI scans employing [(11)C]methionine positron emission tomography (PET) and contrast-enhanced 3D-MR seq...
Article
Full-text available
Background. Cancer in childhood is rare, but nevertheless one of the most frequent causes of disease related death. Initial symptoms are often unspecific, frequently leading to a delay of cancer diagnosis. As a timely diagnosis can be crucial for the clinical outcome, our aim is to point out when unspecific symptoms should be considered suspect of...
Article
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Primary metastatic diffuse intrinsic pontine glioma (DIPG) is relatively rare and associated with a dismal prognosis. Combining craniospinal irradiation (CSI) with concurrent temozolomide and nimotuzumab therapy may slightly improve tumor control and overall survival. However, little is known about the feasibility and toxicity of this treatment app...
Article
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Introduction: Whereas in the adult population 5-Aminolevulinic acid (5-ALA) fluorescence guidance has been widely accepted for improving the extent of tumor resection, the application in children remains an off-label use. Even though most pediatric study protocols require a complete resection for improving outcome parameters, only few pediatric pa...
Article
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The amplification of putative oncogenes is a common finding within the genome of various cancer types. Identification and further targeting of specific junction sites within the sequence of genomic amplicons (amplicon fusion sites, AFS) by PCR (AFS-PCR) is suitable for quantification of minimal residual disease (MRD). This approach has recently bee...
Data
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Figure S2. PCR efficiencies tested by a 1:10 dilution series of DNA isolated from bone marrow at time of initial diagnosis. (A) Ct-values, melting curve and calculated efficiency of the INHBB-PCR. (B) Ct-values, melting curve and calculated efficiency of the AFS-PCR. PCR efficiencies were calculated by the BioRad IQ5-Software (Version 2.1.97.1001).
Data
Table S1. Amplified and deleted chromosomal regions and the genes covered by these aberrations in the investigated case of ALL identified by whole genome array (Affymetrix Cytogenetics Whole-Genome 2.7M Array).
Data
Table S2. Comparison of the calculated MRD values estimated by FACS analysis, IgH/TCR-qPCR and AFS-PCR. (a) Blast count in bone marrow samples from initial diagnosis, day 15 (d15) and day 29 (d29) after the beginning of therapy. (b) Blast count in bone marrow samples from initial diagnosis and recurrent disease at days 513 (d513) and 533 (d533).
Data
Full-text available
Figure S1. Ct-Values (left column) and melting curves (right column) of the INHBB and the AFS-PCR fragments. (A) First row: INHBB-PCR and second row: AFS-PCR for detection of MRD values on day 513 (blue) and day 533 (red) including a AFS-negative control DNA (human placenta) (purple). (B) First row: INHBB-PCR and second row: AFS-PCR for detection o...
Article
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Zusammenfassung Im Kindesalter stellen Hirntumoren nach den Leukämien die zweitgrößte Gruppe maligner Erkrankungen dar. Die ersten Symptome sind meist unspezifisch und sehr heterogen, abhängig von der Tumorlokalisation und vom Alter des Patienten. Psychische Veränderungen finden sich nicht nur am häufigsten überhaupt bei Patienten mit einem Hirntum...
Article
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In the last decades the chances of surviving childhood cancer have increased. Nowadays psychological and psychosocial long term side effects become more spotlighted. Especially the posttraumatic stress disorder is focused at the moment as a possible side effect of childhood cancer. Cancer as a life-threatening illness is unpredictable and associate...
Article
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Reliable diagnostic strategies for individuals with cancer demand practical methods for highly sensitive and specific detection of tumor cells. Amplification of genomic regions that include putative oncogenes is common in tumor cells of various types. Genomic array platforms offer the opportunity to identify and precisely map amplified genomic regi...
Article
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Hochsensitive Verfahren zum direkten Nachweis von Tumorzellen und tumorzellspezifischen Veränderungen auf Genom-(DNA) oder Genexpressions(mRNA)-Ebene halten zunehmend Einzug in die klinische Praxis. Aktuell sind zytologische und molekulargenetische, insbesondere PCR-basierte Methoden, wichtige diagnostische Bausteine im Rahmen der Untersuchung auf...
Article
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To evaluate the impact of a predefined gene expression-based classifier for clinical risk estimation and cytotoxic treatment decision making in neuroblastoma patients. Gene expression profiles of 440 internationally collected neuroblastoma specimens were investigated by microarray analysis, 125 of which were examined prospectively. Patients were cl...
Article
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Zusammenfassung Zu den in der pädiatrischen Onkologie wesentlichen höhergradigen gliomatösen Tumoren zählen das anaplastische Astrozytom, das maligne Hirnstammgliom sowie das Glioblastom. Trotz derzeitiger multimodaler Therapie, bestehend aus Tumorresektion, Bestrahlung und zytostatischer Therapie, ist die Prognose der betroffenen Kinder schlecht....
Chapter
Das Neuroblastom ist eines der interessantesten Malignome im Kindesalter und stellt sowohl für den Kliniker als auch für den Forscher eine Herausforderung dar. Das Spektrum der klinischen Verläufe reicht von spontaner Regression ohne zytostatische Therapie über Ausdifferenzierung in situ bis zur Progression trotz maximaler Therapieintervention.
Article
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MYCN amplification is an important therapy-stratifying marker in neuroblastoma. Fluorescence in situ hybridization with signal detection on the single-cell level allows a critical judgement of MYCN intratumoral heterogeneity. The MYCN status was investigated by fluorescence in situ hybridization at diagnosis and relapse. Heterogeneity was defined a...
Article
MicroRNAs constitute a family of small RNA species that regulate translation and stability of mRNA. This additional layer of epigenetic regulation has escaped discovery until recently, and introduces another level between mRNA expression profiling and proteomics. Since microRNAs are involved in regulating most, if not all cellular processes, their...
Article
In human neuroblastoma, amplification of the MYCN gene predicts poor prognosis and resistance to therapy. In a shRNA screen of genes that are highly expressed in MYCN-amplified tumors, we have identified AURKA as a gene that is required for the growth of MYCN-amplified neuroblastoma cells but largely dispensable for cells lacking amplified MYCN. Au...
Article
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Schlüsselwörter Appendixkarzinoid, neuroendokrine Tumo-ren, MEN 1, Appendektomie, Karzinoidsyn-drom, Chromogranin A (CgA), 5-Hydroxyin-dolessigsäure (5 HIAA), DOTATOC-PET Zusammenfassung Das Appendixkarzinoid (AC) ist der häufigste neuroendokrine Tumor und wird als Zufallsbe-fund bei 0,3 % aller Appendektomien gefun-den. Die Prognose ist sehr gut,...
Article
Zusammenfassung Das Appendixkarzinoid (AC) ist der häufigste neuroendokrine Tumor und wird als Zufallsbefund bei 0,3 % aller Appendektomien gefunden. Die Prognose ist sehr gut, die 5-JahresÜberlebensrate beträgt 99 %. Das AC kann im Rahmen von MEN 1 (Wermer-Syndrom) auftreten. Bei einer Größe des AC unter 2 cm treten äußerst selten Metastasen auf....
Article
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In response to stimuli that activate p53, cells can undergo either apoptosis or cell cycle arrest, depending on the precise pattern of p53 target genes that is activated. We show here that Zbtb4, a transcriptional repressor protein, associates with the Sin3/histone deacetylase co-repressor and represses expression of P21CIP1 as part of a heterodime...
Article
MYCN amplification is a common feature of aggressive tumour biology in neuroblastoma. The MYCN transcription factor has been demonstrated to induce or repress expression of numerous genes. MicroRNAs (miRNA) are a recently discovered class of short RNAs that repress translation and promote mRNA degradation by sequence-specific interaction with mRNA....
Conference Paper
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N-Myc is an oncogenic transcription factor that has a key role in driving cell proliferation in neuronal cells. In neuronal progenitors, N-Myc is degraded in a cell cycle and growth factor-dependent manner. Specifically, degradation of N-Myc requires sequential phosphorylation of serine 62 by cyclin B/Cdk1 complexes and of threonine 58 by Gsk3. Pho...
Conference Paper
Full-text available
There are several different algorithms published for the identification of differentially expressed genes in DNA microarray experiments. Such algorithms produce ordered lists of genes. To compare the performance of these algorithms established measurements from Information Retrieval are proposed. A benchmark data set with known properties is genera...
Chapter
Im Kindesalter sind primäre Tumoren des Respirationstraktes sehr selten. Die meisten Neoplasien des Lungenparenchyms sind Metastasen. In die Lunge metastasierende Tumoren bei Kindern sind: der Wilms-Tumor, das Osteosarkom, Ewing-Sarkom, Rhabdomyosarkom und Hepatoblastom.
Article
Full-text available
Fifteen percent to 20% of human neuroblastomas show amplification of the MYCN oncogene physiologically located at chromosome 2p24-25, indicating an aggressive subtype of human neuroblastoma with a poor clinical outcome. Recent findings revealed that the structure of the amplicon differs interindividually and that coamplification of genes in telomer...
Article
Full-text available
Inactivation of caspase-8 because of aberrant gene methylation has been associated with amplification of the MYCN oncogene and aggressive disease in neuroblastoma, suggesting that caspase-8 may function as tumor suppressor. However, the prognostic effect of caspase-8 in neuroblastoma has remained obscure. Therefore, we investigated caspase-8 expres...
Article
Full-text available
The oncogene BMI1 encodes a polycomb group transcription factor that is required for embryonic development and self-renewal of stem cells. Despite these important functions little is known about the regulation of BMI1 expression. A cDNA microarray based search for target genes of E2F-1 in neuroblastoma cells expressing a 4-OHT-regulated E2F-1-ER fu...
Conference Paper
Amplification of N-MYC is one of the most important genetic aberrations in neuroblastoma and strongly correlates with poor outcome. Previous microarray analysis of 94 primary human neuroblastomas identified over 100 genes deregulated in N-MYC amplified tumors. However, the contribution of these genes to tumorigenesis remained largely unclear. In o...
Article
Full-text available
Neuroblastoma is a common childhood tumor comprising cases with rapid disease progression as well as spontaneous regression. Although numerous prognostic factors have been identified, risk evaluation in individual patients remains difficult. To define a reliable prognostic predictor and gene signatures characteristic of biological subgroups, we per...