Hiroshi Funaki

Hiroshi Funaki
Ehime University · United Graduate School of Agricultural Sciences

Master of Agriculture


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I am currently studing at the Department of Agriculture, Kochi University. My research interest is marine toxic dinoflagellate Gambierdiscus, isolation, abundance survey using qPCR, growth experiments for temperature, salinity and light intensity, toxicity test using mouse bioassay, etc. Now I am interested in Bioinformatics, Microbiology and Marine Biology. Our most recent publication is 'The effects of light intensity on the growth of Japanese Gambierdiscus spp. (Dinophyceae)', I cooperated these growth experiments and mesured vertical light intensity at sampling site. I also have interesting to get post doctoral job at abroad after finished my Ph D.


Publications (6)
The genus Gambierdiscus is a marine benthic/epiphytic dinoflagellate that has been investigated worldwide as the causative agent of ciguatera poisoning (CP). In Japan, CP occurs mainly in the subtropical region and sporadically in the temperate region. To understand the mechanism of CP outbreaks in the coastal regions, identifying the species of Ga...
The genus Gambierdiscus is a marine benthic/epiphytic dinoflagellate considered the causative agent of ciguatera poisoning (CP). Clarifying the geographical distribution of this genus to understand the potential risk of CP is important. Many studies have focused only on the species/phylotype composition of Gambierdiscus in shallow waters, but no st...
Macroalgal samples were collected from coastal waters in subboreal to subtropical zones in Japan (< 3–30 m depths) and 32 clonal strains of non-motile dinoflagellate-like protists were established. Molecular phylogenetic analyses of the LSU rDNA D1/D2, SSU rDNA, ITS region, and concatenated SSU rDNA + LSU rDNA D1/D2 sequences revealed that the stra...
Diarrhetic shellfish poisoning (DSP) is caused by the consumption of shellfish contaminated by diarrhetic shellfish toxins (DSTs) such as okadaic acid (OA) and dinophysistoxins (DTXs) produced by some species of dinoflagellates. To prevent the occurrence of human intoxication cases, inspection of DSTs (OA and DTXs) in shellfish is important. An ins...
In the present study, the abundance of Prorocentrum and the molecular phylogeny, distribution, and DST production of P. lima complex and P. caipirignum in Japan were investigated. First, the cell densities of Prorocentrum were assessed from the temperate to subtropical zones in Japan between 2014 and 2018. The cell density in the subtropical zone [...
Marine toxic dinoflagellates of the genus Gambierdiscus are the causative agents of ciguatera fish poisoning (CFP), a form of seafood poisoning that is widespread in tropical, subtropical and temperate regions worldwide. The distributions of Gambierdiscus australes, Gambierdiscus scabrosus and two phylotypes of Gambierdiscus spp. type 2 and type 3...


Cited By


Project (1)
Microalgae have dominated almost all living habitat on the planet, and their contribution as primary producers. Some of the members of the microalgae are known to produce toxins, which play a negative role in the health and environment. In the present projects, I focus on the community assemblages at aquaculture farms, with special emphasis on the HAB species. Diversity assessment is conducted using metabarcoding approach. The main aim is to understand the bloom dynamics of red-tide causative algae. Vertical distribution of the red-tide causative algal cysts in cores are assessed to interpret the anthropogenic event and changes in the community structure with respect to climate change. Furthermore, quantitative PCR is used to enumerate a dominant target species and then estimate their abundance in each sample or cores.