Hinnerk Schulz-Hildebrandt

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• PhD
• Instructor in Dermatology at Harvard Medical School

Intravital microscopy (IVM) offers the opportunity to visualize static and dynamic changes of tissue on a cellular level. It is a valuable tool in research and may considerably improve clinical diagnosis. In contrast to confocal and non-linear microscopy, optical coherence tomography (OCT) with microscopic resolution (mOCT) provides intrinsically c...

Purpose: Corneal neovascularization, in particular lymphangiogenesis, is a limiting factor in corneal transplant survival. Novel treatment approaches focus on (selective) inhibition and regression of lymphatic vessels. Imaging clinically invisible corneal lymphatic vessels is a prerequisite for these strategies. Using a murine model, this study inv...

While navigation and interventional guidance are typically based on image data, the images do not necessarily reflect mechanical tissue properties. Optical coherence elastography (OCE) presents a modality with high sensitivity and very high spatial and temporal resolution. However, OCE has a limited field of view of only 2–5 mm depth. We present a...

Sensorineural hearing loss (SNHL) is caused by damage to the mechanosensory hair cells and auditory neurons of the cochlea. The development of imaging tools that can directly visualize or provide functional information about a patient’s cochlear cells is critical to identify the pathobiological defect and determine the cells’ receptiveness to emerg...

Mucus stasis is a pathologic hallmark of muco-obstructive diseases, including cystic fibrosis (CF). Mucins, the principal component of mucus, are extensively modified with hydroxyl (O)-linked glycans, which are largely terminated by sialic acid. Sialic acid is a negatively charged monosaccharide and contributes to the biochemical/biophysical proper...

Mucus stasis is a pathologic hallmark of muco-obstructive diseases, including cystic fibrosis (CF). Mucins, the principal component of mucus, are extensively modified with hydroxyl (O)-linked glycans, which are largely terminated by sialic acid. Sialic acid is a negatively charged monosaccharide and contributes to the biochemical/biophysical proper...

Accurate assessment of corneal curvatures using frequency domain optical coherence tomography (OCT) with galvanometer scanners remains challenging due to the well-known scan field distortion. This paper presents an algorithm and software for correcting the distortion using only two simple measurements in which a readily available standard sphere is...

Circumferential scanning in endoscopic imaging is crucial across various disciplines, and optical coherence tomography (OCT) is often the preferred choice due to its high-speed, high-resolution, and micron-scale imaging capabilities. Moreover, real-time and high-speed 3D endoscopy is a pivotal technology for medical screening and precise surgical g...

Hypothesis: Otitis media (OM) can be successfully visualized and diagnosed by dynamic microscopic optical coherence tomography (dmOCT). Background: OM is one of the most common infectious diseases and, according to the WHO, one of the leading health problems with high mortality in developing countries. Despite intensive research, the only definitiv...

Asthma is one of the most common chronic diseases. Mucus overproduction is consistently linked to asthma morbidity and mortality. Despite the knowledge of the importance of mucus, little data exists on how mucus is transported in asthma and the immediate effects of therapeutic intervention. We therefore used microscopic optical coherence tomography...

Purpose: Microscopic optical coherence tomography (mOCT) has an imaging resolution of 1 µm in all voxel dimensions, but individual epithelial cells are difficult to resolve due to lack of scattering contrast. Adding dynamic contrast processing to mOCT (dmOCT) results in color images that enable visualization of individual cells and possibly give i...

In the imaging of airway tissue, optical coherence tomography (OCT) provides cross-sectional images of tissue structures, shows cilia movement and mucus secretion, but does not provide sufficient contrast to differentiate individual cells. By using fast sequences of microscopic resolution OCT (mOCT) images, OCT can use small signal fluctuations to...

Imaging airway tissue, optical coherence tomography (OCT) provides cross-sectional images of tissue structures, shows cilia movement and mucus secretion, but does not provide sufficient contrast to differentiate individual cells. By using fast sequences of microscopic resolution OCT (mOCT) images, OCT can picture small signal fluctuations (dynamic)...

Purpose The purpose of this study was to evaluate the feasibility and accuracy of a radiation-free implantation of a thoracic aortic stent-graft employing fiberoptic and electromagnetic tracking in an anthropomorphic phantom. Materials and Methods An anthropomorphic phantom was manufactured based on computed tomography angiography (CTA) data from...

Purpose To assess the efficacy of an instrument-integrated OCT-based (iiOCT) distance sensor during robotic vitreo-retinal surgery using the Preceyes surgical system (PSS). Design Single center, interventional study. Participants Patients requiring vitreo-retinal surgery. Methods Five patients were enrolled. Standard pre-operative OCT (pOCT) ima...

Volumetric imaging of dynamic processes with microscopic resolution holds a huge potential in biomedical research and clinical diagnosis. Using supercontinuum light sources and high numerical aperture (NA) objectives, optical coherence tomography (OCT) achieves microscopic resolution and is well suited for imaging cellular and subcellular structure...

Background In endovascular aortic repair (EVAR) procedures, medical instruments are currently navigated with a two-dimensional imaging based guidance requiring X-rays and contrast agent. Methods Novel approaches for obtaining the three-dimensional instrument positions are introduced. First, a method based on fiber optical shape sensing, one electr...

In endovascular aneurysm repair (EVAR) procedures, the stent graft navigation and implantation is currently performed under a two-dimensional (2D) imaging based guidance requiring X-rays and contrast agent. In this work, a novel 3D stent graft guidance approach based on tracking systems is introduced. A calibration method and the visualization of t...

During endovascular aneurysm repair (EVAR) procedures, medical instruments are guided with two-dimensional (2D) fluoroscopy and conventional digital subtraction angiography. However, this guidance requires X-ray exposure and contrast agent administration, and the depth information is missing. To overcome these drawbacks, a three-dimensional (3D) gu...

Caries, the world's most common chronic disease, remains a major cause of invasive restorative dental treatment. To take advantage of the diagnostic potential of optical coherence tomography (OCT) in contemporary dental prevention and treatment, an intraorally applicable spectral-domain OCT probe has been developed based on an OCT hand-held scanner...

While optical coherence tomography (OCT) provides a resolution down to 1 µm, it has difficulties in visualizing cellular structures due to a lack of scattering contrast. By evaluating signal fluctuations, a significant contrast enhancement was demonstrated using time-domain full-field OCT (FF-OCT), which makes cellular and subcellular structures vi...

Purpose: To evaluate a new non-ablative and adjustable procedure for laser ablative refractive corneal surgery in hyperopia using the injection of a biocompatible liquid filler material into a stromal pocket. Methods: A total of 120 stromal pockets were created using a clinical femtosecond laser system in 96 rabbit corneoscleral discs and 24 who...

Purpose: During endovascular aneurysm repair (EVAR) procedures, medical instruments are guided with two-dimensional (2D) fluoroscopy and conventional digital subtraction angiography. However, this requires X-ray exposure and contrast agent is used, and the depth information is missing. To overcome these drawbacks, a three-dimensional (3D) guidance...

Two‐photon microscopy (2PM) has brought unique insight into the mechanisms underlying immune system dynamics and function since it enables monitoring of cellular motility and communication in complex systems within their genuine environment—the living organism. However, use of 2PM in clinical settings is limited. In contrast, optical coherence tomo...

Currently, fluoroscopy and conventional digital subtraction angiography are used for imaging guidance in endovascular aortic repair (EVAR) procedures. Drawbacks of these image modalities are X-ray exposure, the usage of contrast agents and the lack of depth information. To overcome these disadvantages, a catheter prototype containing a multicore fi...

While optical coherence tomography (OCT) provides a resolution down to 1 micrometer it has difficulties to visualize cellular structures due to a lack of scattering contrast. By evaluating signal fluctuations, a significant contrast enhancement was demonstrated using time-domain full-field OCT (FF-OCT), which makes cellular and subcellular structur...

In endovascular aortic repair (EVAR) procedures fluoroscopy and conventional digital subtraction angiography are currently used to guide the medical instruments inside the patient. Drawbacks of these methods are X-ray exposure and the usage of contrast agents. Moreover, the fluoroscopy provides only a 2D view, which makes the guidance more difficul...

Airway mucus obstruction is a hallmark of chronic lung diseases such as cystic fibrosis, asthma and COPD, and the development of more effective mucus mobilizing therapies remains an important unmet need for patients with these muco-obstructive lung diseases. However, methods for sensitive visualization and quantitative assessment of immediate effec...

Background/objectives: Standard optical coherence tomography angiography (OCTA) has been limited to imaging blood vessels actively undergoing perfusion, providing a temporary picture of surface microvasculature. Capillary perfusion in the skin is dynamic and changes in response to the surrounding tissue's respiratory, nutritional, and thermoregula...

Purpose Endovascular aortic repair procedures are currently conducted with 2D fluoroscopy imaging. Tracking systems based on fiber Bragg gratings are an emerging technology for the navigation of minimally invasive instruments which can reduce the X-ray exposure and the used contrast agent. Shape sensing of flexible structures is challenging and inc...

In order to fully exploit the diagnostic potential of optical coherence tomography (OCT) in contemporary restorative dentistry, an intraorally applicable OCT probe has been constructed. The probe was connected to a commercially available OCT system. The handling of the probe and the quality of the OCT images were optimized and evaluated on human ex...

Purpose: Endovascular aortic repair procedures are currently conducted with 2D fluoroscopy imaging. Tracking systems based on fiber Bragg gratings are an emerging technology for the navigation of minimal-invasive instruments which can reduce the x-ray exposure and the used contrast agent. Shape sensing of flexible structures is challenging and incl...

Optical coherence tomography angiography (OCTA) provides in-vivo images of microvascular perfusion in high resolution. For its application to basic and clinical research, an automatic and robust quantification of the capillary architecture is mandatory. Only this makes it possible to reliably analyze large amounts of image data, to establish biomar...

Non-invasive imaging to qualify and quantify inflammatory processes on the ocular surface may enable early diagnosis as well as optimized therapy. Aim of this study was to test the hypothesis that functionalized nanoparticles and optical coherence tomography (OCT) can visualize inflammation of ocular surface tissues. In order to detect the particle...

We present a forward-viewing fiber scanning endoscope (FSE) for high-speed volumetric optical coherence tomography (OCT). The reduction in size of the probe was achieved by substituting the focusing optics by an all-fiber-based imaging system which consists of a combination of scanning single-mode fibers, a glass spacer, made from a step-index mult...

Purpose: Retinal vein cannulation is an experimental procedure during which a clot-dissolving drug is injected into an obstructed retinal vein. However, due to the fragility and minute size of retinal veins, such procedure is considered too risky to perform manually. With the aid of surgical robots, key limiting factors such as: unwanted eye rotati...

Purpose: Retinal vein cannulation is an experimental procedure during which a clot-dissolving drug is injected into an obstructed retinal vein. However, due to the fragility and minute size of retinal veins, such procedure is considered too risky to perform manually. With the aid of surgical robots, key limiting factors such as: unwanted eye rotat...

Vitreoretinal surgery concerns a set of particularly demanding minimal invasive micro-surgical interventions at the retina. Micro-surgeons are targeting sub-millimeter-sized structures here. Tiny vessels or wafer-thin membranes are to be cannulated or need to be peeled off. The greatest care is to be displayed not to damage these fragile structures...

Den Schwerpunkt dieses Artikels bildet die Vermittlung praktischen Wissens rund um die optische Koharenztomografie (OCT). Wahrend in Teil 11 die physikalischen Grundlagen vorgestellt wurden, werden hier praktische Aspekte fur den Anwender erlautert, unabhangig vom verwendeten Geratetyp oder Hersteller. Grundsatzliche Parameter und Einstellmoglichke...

Die optische Koharenztomografie (OCT) ist ein in vielen Bereichen der Augenheilkunde etabliertes Bildgebungsverfahren. Im 1. Teil zum Thema OCT werden technisch-physikalische Grundlagen zum Verstandnis vermittelt. Die prinzipielle Arbeitsweise der OCT wird erlautert. Die zugrundeliegende Licht-Gewebe-Wechselwirkung sowie die OCT-Messtechnik werden...

Mucus transport is essential to remove inhaled particles and pathogens from the lung. Impaired removal of mucus often results in worsening of lung diseases. To understand the mechanisms of mucus transport and to monitor the impact of therapeutic strategies, it is essential to visualize airways and mucus in living animals without disturbing transpor...

Having a noninvasive technique to assess the human airway wall with subcellular resolution directly in patients would be of great benefit for the assessment of airway diseases. We therefore developed an endoscopic optical coherence microscope (eOCM) and tested its performance in human subjects. Images were taken with a custom-built eOCM device cons...

High resolution imaging of human airways holds promise to visualize pathologic morphology and function with subcellular resolution. We have shown that endoscopic optical coherence microscopy (eOCM) is able to provide cross-sectional images with micrometer resolution. Our eOCT device is based on a supercontinuum light source, a custom made 400nm spe...

A microscope-based optical coherence tomography (OCT) device was used to assess the microanatomy of the tympanic membrane in patients with chronic myringitis. A prospective study was designed for this purpose. OCT measurements of the tympanic membrane were done on 11 patients with myringitis with a microscope-based spectral domain OCT system. The i...

Die optische Koharenz-Tomografie ist ein vielversprechendes Verfahren fur die Atemwegsdiagnostik. Allerdings fehlt ihr die zellulare Auflosung. Um eine zellulare Auflosung zu erreichen, haben wir konfokale Mikroskopie und optische Koharenz-Tomografie in einem optischen Koharenz-Mikroskop (OCM) vereint. Fur ex vivo OCM wurde die Trachea der Maus in...