
Hien Van Doan- Ph.D.
- Professor (Associate) at Chiang Mai University
Hien Van Doan
- Ph.D.
- Professor (Associate) at Chiang Mai University
Associate Professor at Chiang Mai University
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327
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Introduction
Current institution
Publications
Publications (327)
The present study investigates the effects of combined or singular administration of low molecular weight sodium alginate (LWMSA) and Lactobacillus plantarum on innate immune response, disease resistance and growth performance of Nile tilapia (Oreochromis niloticus). Three hundred and twenty fish were supplied and randomly stocked in sixteen glass...
This study investigates the effect of Sargassum ilicifolium algae fermentation using Lactobacillus plantarum and Saccharomyces cerevisiae probiotics on growth, immunity, gene expression, and digestive and antioxidant enzymes before and after challenge with Streptococcus iniae in trout. Trout (50.69 ± 1.33 g) were fed with different percentages of S...
Background: Microalgae and macroalgae play significant roles in aquaculture, offering various benefits that
contribute to sustainable practices in the industry.
Methods: The aim of this research was to investigate the impact of feeding Lates calcarifer a combination of
Sargassum ilicifolium and Spirulina platensis on growth, immune responses, and t...
Novel and natural alternative feed ingredients are essential for sustainable aquaculture sector. For a period of 60 days, this study examined the effects of incorporating Hollyhock (Alcea rosea) powder into the diet on gene expressions, growth parameters, serum biochemical and innate immune parameters, as well as antioxidant capacity in zebrafish (...
Aquaculture is playing a pivotal role in meeting the growing global request for seafood products. One of the important aspects of aquaculture management is using of high-quality ingredients to improve the performance of fish and crustaceans. One of the main priorities of nutrition scientists over the past decades is to partially or totally replace...
Selenium plays a crucial role in vital physiological processes for fish, by involved in many processes of cellular metabolism and increase the growth performance and health status of fish. Nano-selenium (nano-Se) has been shown to promote fish growth more than other traditional inorganic Se forms. Additionally, increased the capacity to absorb vari...
Rice is not a horticultural crop, thus the idea that indoor cultivation cannot compete with outside production in desert and non-urban areas with shortages of water and challenging cultivation circumstances. The study intends to offer critical crops to poor communities through small-scale aquaponics systems that can adapt to climate change, despite...
Recently, aquaculture has some limitations, such as the limited availability of land, water, feed ingredients, and biosecurity concerns. Managing economic activity has become mandatory to confront global climate change. In the last few decades, fish meal recognized the bestsource of protein for aquafeeds; though, the shortage of fish meal supply an...
Antarctic krill meal (KM) (Euphausia superba) as a substitute for fishmeal in aquatic animal diets is gaining popularity worldwide. A quantitative approach investigating the efficacy of using this protein on the production performance of aquatic animals remains widely limited. Here, we employed a meta-analysis to quantify the overall effects (Hedge...
This study evaluates the impact of mango peel-derived pectin and Bacillus iranensis on the growth performance, immune response, histology, gene expression, and ammonia resistance of Nile tilapia (Oreochromis niloticus). A total of 320 fingerlings (average weight 30.5 ± 0.014 g) were divided into four groups and fed different diets for eight weeks:...
Common Carp (Cyprinus carpio) exposure to water-soluble fraction of crude oil (WSF): Evaluation of protective effects of dietary Rosmarinus officinalis leaf on water quality, growth performance, oxidative stress, stress hormones and heat shock proteins
The study, herein, investigated the effects of the inclusion of longan peel (LP) powder in the diet of Nile Tilapia (Oreochromis niloticus), focusing on comparative evaluations of growth performance, immunity, and immune-antioxidant related gene expressions. For this purpose, a total of 300 healthy fish (average initial weight: 13.70 ± 0.06 g) were...
With the intensification of aquaculture to meet the rising demands of fish and shellfish, disease outbreaks during the larval and adult stages are a major challenge faced by aqua culturists. As the prophylactic use of vaccines and antibiotics has several limitations, research is now focused on sustainable alternatives to vaccines and antibiotics, e...
This study explored the effects of mango seed (MS) powder supplementation on the growth, immune response, gene expression, and intestinal morphology of Nile tilapia (Oreochromis niloticus) over an 8-week period. A total of 300 Nile tilapia fingerlings (average weight of 15.29 ± 0.05 g) were divided into five treatment groups and fed either a basal...
The aquaculture industry is increasingly pursuing sustainable and effective alternatives to traditional fishmeal and fish oil-based feeds due to their economic and ecological drawbacks. Red yeast (Sporidiobolus pararoseus) has demonstrated potential as a functional feed additive due to its bioactive compounds, which can boost growth and immunity in...
This study evaluated the effects of dietary supplementation with different doses (0, 10, 20, 40, and 80 g kg-1) of powdered avocado seed (AS) on the growth performance, immunological response, and gene expression of Nile tilapia (Oreochromis niloticus) reared in a biofloc system over 8 weeks. A total of 300 Nile tilapia fingerlings (average weight...
This study explores the effects of dietary supplementation with passion fruit peel pectin (Passiflora edulis) and red yeast cell walls (Sporidiobolus pararoseus) on growth performance, immunity, intestinal morphology, gene expression, and gut microbiota of Nile tilapia (Oreochromis niloticus). Nile tilapia with an initial body weight of approximate...
This study provides a comprehensive summary of the findings regarding the application and diagnostic efficacy of droplet digital PCR (ddPCR) in detecting viral and bacterial pathogens in aquaculture. Utilizing a systematic search of four databases up to 6 November 2023, we identified studies where ddPCR was deployed for pathogen detection in aquacu...
glucan exerts a positive impact and consider as a functional feed additives to enhance fish growth, immunity, control disease outbreaks and pathogen infections by increasing functional, immune and decreasing harmful responses. The present study aimed to determine the most effective dietary level of beta-glucan (βG) to improve Nile tilapia Oreochrom...
The current work investigates the prospective applications of Artificial Intelligence (AI) in the aquaculture industry. AI depends on collecting, validating, and analyzing data from several aspects using sensor readings, and feeding data sheets. AI is an essential tool that can monitor fish behavior and increase the resilience and quality of seafoo...
Organic acid salts can modulate fish growth performance and antioxidant/immune systems, thus they can be used as feed additives for intensive fish culture. Hence, this study investigated the effects of dietary sodium butyrate (NaB) supplementation on growth, antioxidant, immunological and stress indicators in common carp, Cyprinus carpio, reared at...
Probiotics are helpful bacteria that safeguard host animals from harmful pathogens. In fish farming, the primary aim of using probiotics is to preserve or reestablish a balance between pathogenic microorganisms and the native bacteria that constitute the intestinal and skin microbial communities of fish. This study investigates the effects of host-...
In the current study, the effects of dietary fulvic acid supplementation at levels of 0.5, 1 and 2% were examined in white-leg shrimp, Litopenaeus vannamei. A significant increase in the weight of the shrimp was observed in the group treated with 2% fulvic acid in comparison to the control group. This may have been associated with an increased dige...
This study examines the use of dragon fruit peel (DFP) powder as a dietary supplement on growth performance, immune responses, and gene expression of Nile tilapia, Oreochromis niloticus, cultured within biofloc systems. A total of 300 Nile tilapia fingerlings (14.64 ± 0.09 g) were subjected to five dietary treatments, with DFP added to a basal diet...
Treatment of skin wounds with diverse pathological characteristics presents significant challenges due to the limited specific and efficacy of current wound healing approaches. Microneedle (MN) patches incorporating bioactive and stimulus materials have emerged as a promising strategy to overcome these limitations and integrating bioactive material...
This study aimed to conduct a systematic review and meta-analysis evaluating the inclusion of β-glucan in aquaculture animal diet and its impact on their health outcomes. Relevant studies were identified from Scopus and Web of Science databases. A total of 82 primary studies published between 1996 and 2024 were reviewed, of which 70 were included i...
Fucoidan (Fuc) is a sulfated polysaccharide derived from brown algae and has various biological activities such as immune modulator, growth enhancer, antioxidant and bactericidal. However, there is no information about the effect of fucoidan on ornamental fish. This study investigated the effect of fucoidan from brown algae Sargassum ilicifolium on...
A makiang seed powder (MSP) feeding trial was performed on Nile tilapia (Oreochromis niloticus) for eight weeks to evaluate the effects on growth performance, immune response, and gene expression. Three hundred fingerlings (13.20 ± 0.04 g) were fed five diets with various amounts of MSP at 0, 5, 10, 20 and 40 g kg−1. After four and eight weeks of t...
Chitosan (CH) shows great potential as an immunostimulatory feed additive in aquaculture. This study evaluates the effects of varying dietary CH levels on the growth, immunity, intestinal morphology, and antioxidant status of Nile tilapia (Oreochromis niloticus) reared in a biofloc system. Tilapia fingerlings (mean weight 13.54 ± 0.05 g) were fed d...
The role of sugarcane bagasse (SCB) as a cheap carbohydrate (CHO) source was evaluated in L. vannamei larvae on the zootechnical indices and the countenance of growth, immune and antioxident genes in the liver and muscle tissues. The experiment includes two treatments, the control which feeds a commercial diet and the SCB treatment. It continued fo...
The emergence of antibiotic-resistant bacteria (ARBs) and genes (ARGs) in aquaculture underscores the urgent need for alternative veterinary strategies to combat antimicrobial resistance (AMR). These measures are vital to reduce the likelihood of entering a post-antibiotic era. Identifying environmentally friendly biotechnological solutions to prev...
Herbs and their by-products are important traditional medicines and food supplements; they provide numerous beneficial effects for animals. Consequently, probiotics are living cell organisms , nontoxic, and friendly microbes. Probiotics have numerous beneficial activities such as inhibition of pathogens, enhancement of the immune system, growth, di...
Aquaculture is a crucial and rapidly expanding industry in global food production. Fisheries are, also an essential socio-economic activity, providing abundant resources and remarkable prospects. However, due to the deteriorating ecological environment, aquatic animals are often exposed to traumatic conditions and are susceptible to bacterial infec...
The effects of long-term dietary supplementation with sandalwood (Santalum album L.) essential oil (SEO) was investigated on hemato-biochemical biomarkers, immune status, antioxidant capacity, and resistance against Staphylococcus aureus in Nile tilapia (Oreochromis niloticus). Five groups (with four replicates) of O. niloticus (12.60 ± 0.20 g) wer...
Insect meal has shown promise as a potentially sustainable source of nutrients for aquafeeds, offering an alternative to expensive and ecologically undesirable ingredients, in the context of population explosion and climate change. Despite this promising outlook, its effects on fish growth and immune responses remain to be thoroughly investigated....
This study is designed to evaluate the effects of dietary red macroalgae (Galaxaura oblongata) on growth performance, serum, and skin mucus immunological and antioxidant responses in rainbow trout (Oncorhynchus mykiss). For this, rainbow trout were fed diets containing different levels of G. oblongata (0 (ctrl), 0.5 (G1), and 1 (G2) %) for 8 weeks....
Azolla (Azolla pinnata), an aquatic fern, has high amounts of crude protein and essential amino acids and thus could be an economical and plentiful alternative feed source for poultry. In this study we investigated how partial replacement of pelleted concentrate diet with fresh azolla affected growth performance, carcass quality, and oxidative stre...
The aquafeed industry faces many challenges, including reducing the cost of feed by reducing the quantity of fish meal and soybean meal and researching to replace them with other ingredients lower in cost. furthermore, new low-cost feeds must improve growth and feed utilization while also ensuring fish health. During the past few decades, researche...
Considering environmental sustainability and vulnerability to the effects of climate change on fish production, sustainable and adaptable aquaculture systems are necessary”. Biofloc technology (BFT) and recirculating aquaculture systems (RAS) are eco-friendly, water efficient, highly productive intensive farming systems, which are not associated wi...
With the increase in the human population, the need for protein-rich food is increasing. To cope with the increased demand for fish and fish-related products, aquaculture is shifted towards semi-intensive and intensive culture practices. These cultivation practices exacerbate disease outbreaks. Farmers use extensive antibiotics to manage disease ou...
Probiotics are live beneficial bacteria introduced into the gastrointestinal tract through food or water, promoting good health by enhancing the internal microbial balance. Probiotic microbes produce bacteriocins, siderophores, lysozymes, proteases, and hydrogen peroxides, inhibiting the growth of harmful pathogens. Such beneficial bacteria also pr...
The aim of this study was to assess the effects of dietary nutmeg (Myristica fragrans (MF)) on growth, innate immunity parameters of serum and mucus, antioxidant defence, and expression of immune genes in common carp (Cyprinus carpio). Three hundred fish (3.79 ± 0.02 g) in four groups were distributed in twelve glass aquariums with a capacity of 15...
Colorectal cancer (CRC) is the second leading cause of cancer-related death, mostly due to metastatic disease and the fact that many patients already show signs of metastasis at the time of first diagnosis. Current CRC therapies negatively impact patients’ quality of life and have little to no effect on combating the tumor once the dissemination ha...
An 8-week husbandry trial was carried out toexamine the impacts of graded levels of dietary fulvic acid (FA) on growth, stress, and disease resistance of Asian seabass (Lates calcarifer, 38.2 g) juveniles. Five levels of FA were included in a basal diet (470 g kg-1 crude protein, 150 g kg-1 crude lipid) to prepare experimental diets, including: 0 (...
The widespread use of produced metal oxide nanoparticles (NPs) has increased major concerns about their impact on human as well as aquatic animal health. The present study shows that exposure to different concentrations of zinc oxide (ZnO) NPs led to high accumulations of Zn ions in the metabolic organs of fish (liver and gills), resulting in sever...
The aquaculture industry is expanding to meet the daily requirements of humanity from high-quality seafood. In this regard, intensive aquaculture systems are suggested, resulting in high production but being challenged with immunosuppression and disease invaders. Antibiotics were used for a long time to protect and treat aquatic animals; however, c...
Recently, nano feed supplement research has great attention to improving healthy aquatic production and improving the aquatic environment. With the aims of the present study, chemical and green synthesized nanoparticles are characterized by various instrumentation analyses, namely UV-Vis spectrophotometry (UV-Vis), X-ray diffraction (XRD), Fourier...
An 8-week feeding trial was carried out to evaluate the effects of grapevine (Vitis vinifera) leaf extract (GLE) on the growth, oxidative enzymatic activities, immunity, and expression of antioxidant genes in zebrafish (Danio rerio). Three hundred and sixty zebrafish were supplied and fed with different levels of GLE: 0, 0.5, 1, and 2 g kg−1. The d...
Application of dietary feed additives is an excellent approach to improve fish health status under different stressful condition. The current study investigated the roles of different level of dietary gallic acid (GA) on growth performances, biochemical indices, digestive enzymes activity, antioxidant status and immunological responses in common ca...
Probiotics are live beneficial Micro-organism introduced into the gastrointestinal tract through food or water, probiotics confer several benefits and play important roles in improving growth performances, disease resistance, immunity, health status, gut microbiome, and water quality. The present study examined the effects of water additive probiot...
Brassicaceae familyare traditional, medicinal and nutritional value plants. Brassicaceae family produced valuable components to provide different types of beneficial effects for humans and animals such as anti-oxidant, antimicrobial, anticancer, anti-scurvy, anti-inflammatory, anti-hyperglycemic, anti-tumor, anti-diabetes, anti-stomach diseases, an...
The current research investigated the effects of pot marigold (Calendula officinalis) powder on growth, biochemical parameters, digestive enzymes, serum and mucus immune responses, antioxidant defense, and resistance of rainbow trout (Oncorhynchus mykiss) against Yersinia ruckeri. Fish (No. 450, 15.06±0.11 g; mean±SE) were randomly distributed to f...
Simple Summary
In recent decades, trade of ornamental fish has significantly increased. A rise in demand was observed, especially from top importing countries that contributed majorly to the growth of the market. The destructive fishing methods caused a severe impairment of natural and environmental resources. Thus, there is a need to improve ornam...
The current study is designed to assay the efficacy of chlorogenic acid (ChA) in the diet on growth performance, digestive enzyme activity, serum immunological, biochemical, and antioxidant variables, and mucosal immune response as well as disease resistance of rainbow trout (Oncorhynchus mykiss) juveniles. Rainbow trout juveniles received diets su...
This study investigated the effects of savory (Satureja hortensis), dill (Anethum graveolens), and mooseer (Allium hirtifolium) essential oils on growth, digestive, immunity, and antioxidant parameters, as well as resistance to Aeromonas hydrophila in juvenile common carp (Cyprinus carpio). Experiments were performed on 630 fish with an average wei...
Protein deficiency is a major difficulty for human needs in the past few decades, while different types of aquatic species are rich in high quality protein. Therefore, aquaculture is considered as the main developing food production sector globally. Bacterial infections are the main problem for aquaculture, and their outbreaks have a great impact o...
This study was done to evaluate the effect of different quercetin levels on growth performance, immune responses, antioxidant status, serum biochemical factors, and high-temperature stress responses in common carp (Cyprinus carpio). A total number of 216 common carp with an average weight of 27.21 ± 53 g were divided into 12 tanks ( four treatments...
Pineapple is a commodity and economic fruit with a high market potential worldwide. Almost 60 % of the fresh pineapple, such as peels, pulp, crowns and leaves, are agricultural waste. It is noteworthy that the waste has a high concentration of crude fibre, proteins, ascorbic acid, sugars and moisture content. The pineapple waste utilisation in anim...
The present study evaluated nutritional composition, stable isotopic signature, apparent digestibility coefficient (ADCs), and relative contribution of seven insect meals, namely black soldier fly (Hermetia illucens) (BSF), common housefly (Musca domestica) (CHF), yellow mealworm (Tenebrio molitor) (YMW), lesser mealworm (Alphitobius diaperinus) (L...
Million tonnes of agricultural waste are generated annually worldwide. Agricultural wastes possess similar profiles to the main products but are lower in quality. Managing these agricultural wastes is costly and requires strict regulation to minimise environmental stress. Thus, these by-products could be repurposed for industrial use, such as alter...
The current study was designed to examine the impacts of dietary mannan-oligosaccharides (MOS) on growth, hemato-biochemical changes, digestive-antioxidant enzyme activity, immune response, and disease resistance of milkfish (Chanos chanos) fed diets contained MOS i.e. 1g, 2g, and 3g MOS. The growth parameters were significantly influence in milkfi...
The effects of dietary glycine supplementation, 0 (control), 5 (5 GL), and 10 (10 GL) g/kg, have been investigated on growth performance, hematological parameters, erythrocyte antioxidant capacity, humoral and mucosal immunity in common carp, Cyprinus carpio. After eight weeks feeding, the 5 GL treatment exhibited significant improvement in growth...
Carp is an important aquaculture species globally, and the production is expected to increase with the growing market demands. Despite that, disease outbreaks remain a major challenge, impeding the development of sustainable carp farming. Moreover, the application of antibiotics, a common prophylactic agent, can adversely impact public health and t...
The identification and development of a new plant-based feed ingredient as an alternative protein source to FM have gained the interest of the aquafeed industrial players. Therefore, this study aimed to investigate the physical, biochemical, and bacteriological properties of dietary FWM and the impacts on the growth and reproductive performances of...
Vaccines prepared from formalin-killed Streptococcus agalactiae were administered to Nile tilapia (Oreochromis niloticus) via three different routes: immersion in a water-based vaccine, injection with an oil-based vaccine, and as a water-based oral vaccine. All vaccination treatments increased lysozyme and peroxidase activity in skin mucus of Nile...
Colon cancer is one of the leading causes of death worldwide. In recent years, cannabinoids have been extensively studied for their potential anticancer effects and symptom management. Several in vitro studies reported anandamide’s (AEA) ability to block cancer cell proliferation and migration, but evidence from in vivo studies is still lacking. Th...
This study aimed to investigate the effects of mango peel powder (MGPP) on growth, innate immunity, and immune-antioxidant related gene expression of Nile tilapia reared under biofloc system. Three hundred Nile tilapia (average weight 14.78 ± 0.05 g) were distributed into 15 fiber tanks (300 L per tank) assigned to five treatments in triplication....
Medicinal plants are powerful antioxidants which can improve well-being and suppress oxidative stress caused by environmental toxins in aquatic animals. In this regard, the present research was designed to show the potential effects of psyllium (Plantago ovata) seed extract (PSE) on the growth, and immune responses of common carp Cyprinus carpio ex...
This study investigated the influences of dietary supplementation of Lactobacillus plantarum (LP) on the digestive efficiency, growth performance, immunological response, and disease resistance of black sharkminnow (Labeo chrysophekadion) challenged with Aeromonas hydrophila (A. hydrophila). The fish (2.2 ± 0.14 g) were fed different doses of LP su...
The study revealed the potential of fermented soybean meal (FSBM) as a fish meal (FM) re-placement in African catfish (Clarias gariepinus) feed formulation. Five isonitrogenous diets (32% crude protein) were prepared with five different levels of FSBM as FM replacement, namely 0% FSBM (T1), 40% FSBM (T2), 50% FSBM (T3), 60% FSBM (T4) and 70% (T5)....
The aquaculture industry is geared toward intensification and successfully meets half of the world's demand for fish protein. The intensive farming system exposes the animal to the risk of disease outbreaks, which has economic consequences. Antibiotics are commonly used for the health management of aquaculture species. However, this has several dra...
The present study aimed to investigate the effects of the herbaceous plant, Dracocephalum kotschyi, on the immune response, antioxidant enzymes activity, growth performance, and related genes expression in zebrafish (Danio rerio). An in vivo trial was carried out by feeding zebrafish (initial weight of 0.47 ± 0.04 g) with different levels of powder...
Aeromonas hydrophila is a ubiquitous bacterium with various hosts that causes mass mortality in farm-raised fish species and significant economic losses. The current antibiotic treatment is ineffective in controlling this bacterium infection in aquaculture species. Therefore, an evaluation of potential phytobiotics is needed to find an alternative...
Mycotoxin-contaminated feeds may negatively affect broiler chickens' health; hence, a sustainable approach to achieve mycotoxin elimination is necessary. This study aimed to evaluate the efficacy of red yeast (Sporidiobolus pararoseus; RY) as a novel mycotoxin binder in broilers. A total of 1440 one-week-old male broiler chicks were randomly assign...
Streptococcosis, particularly that caused by S. iniae and S. agalactiae, is a major re-emerging bacterial disease seriously affecting the global sustainability of aquaculture development. Despite a wide spread of the disease in aquaculture, few studies have been directed at assessing the in vitro antagonistic activity and in vivo efficacy of medici...
This study evaluated the effects of dietary supplementation of thyme (Thymus vulgaris) essential oil (TVO) on growth performance, digestive enzymes, biochemical parameters, hematological indices, liver enzymes, and pathogen resistance in common carp (Cyprinus carpio). Triplicate groups of fish (15.36 ± 0.10 g) were fed daily with diets supplemented...
Bioactive immunostimulants could be derived from different sources like plants, animals, microbes, algae, yeast, etc. Bioactive immunostimulants are the most significant role to enhance aquatic production, as well as the cost of this method, which is effective, non-toxic, and environment-friendly. These immunostimulants are supportive to increase t...
This study aimed to assess the impacts of dietary supplementation with passionfruit (Passiflora edulis) peel powder (PSPP) on the growth, immune response, and expression of immune and antioxidant-related genes in Nile tilapia (Oreochromis niloticus) maintained in a biofloc system. Fish were fed basal diets supplemented with different doses of PSPP...
The purpose of this study was to evaluate the effects of red yeast (Sporidiobolus pararoseus) produced from crude glycerol, as a by-product of the biodiesel production process, on the growth, innate immunity, expression of immune-related gene, and resistance of Nile tilapia against challenge with Streptococcus agalactiae. Fish were fed diets suppli...
This study examined research and development of the commercially important marine lobster, in terms of descriptive metadata and scientometric analysis. We consider a collection of over 9,578 related articles to assess literature patterns and research development trends related to marine lobsters as a topic. Web of Science Core Collection was used t...
Huge amounts of non-edible by-products could be generated from fruit industrial processes. They consist mainly of peels together with low amounts of pulp and seeds. These by-products pose an environmental hazard due to soil, air, and water pollution. Moreover, treating these by-products is very expensive and under strict governmental regulations. N...
The present study was designed to investigate the effect of Spirulina platensis (SP) supplemented diets on growth performance, digestive enzymes, hepatic antioxidants and innate immunity biomarkers in juvenile grass carp (Ctenopharyngodon idella). Two hundred and forty grass carp juveniles (average weight 4.81 ± 0.13 g) were divided into four treat...
The present trial investigates the effects of soil-derived Streptomyces chartreusis KU324443 as a probiotic on growth performance, mucosal and serum immune parameters, and immune and antioxidant-associated gene expression in common carp (Cyprinus carpio). In a two-month experiment, carps were fed with varying levels of S. chartreusis KU324443 (0 (c...
This study investigated the effects of dietary supplementation with anthocyanin extracted from black rice bran (AR) on the growth rate, immunological response, and expression of immune and antioxidant genes in Nile tilapia raised in an indoor biofloc system. A total of 300 Nile tilapia fingerlings (15.14 ± 0.032 g) were maintained in 150 L tanks an...
The current perspective is a pioneer to assess the efficacy of Salvia officinalis leave powder (SOLP) on growth, intestinal enzymes, physiological and antioxidant status, immunological response, and gene expression of Common carp (Cyprinus carpio). We also looked into fish resistance after being challenged with Aeromonas sobria, a pathogenic zoonot...
The aquafeed industry continues to expand in response to the rapidly growing aquaculture sector. However, the identification of alternative protein sources in aquatic animal diets to replace conventional sources due to cost and sustainability issues remains a major challenge. Recently, insects have shown tremendous results as potential replacers of...
Individual and combined efficacy of chitooligosaccharides (COS) and alginic acid (AA) at 1 g, 2 g, and 3 g per kg diet was assessed on growth and disease resistance in silver carp (Hypophthalmichthys molitrix) against Edwardsiella ictaluri. Growth parameters including specific growth rate (SGR), weight gain (WG), and feed conversion rate (FCR) were...
The present trial conducted to examine the impact of dietary red seaweeds, Sargassum dentifolium polysaccharides supplementation on growth induces, hematological and histological indicators of the hybrid red tilapia Oreochromis mossambicus × Oreochromis niloticus fingerlings. A total of 240 hybrid red tilapia fingerlings with an average body weight...
Repeat breeding is a substantial problem in heifer and cow breeding leading to greater infertility for female dairy herds. The aim of present study was to investigate the impact of corpus luteum (CL) presence and category and the first gonadotropin-releasing hormone (GnRH) administration concurrent with exogenous progesterone (P4) treatment on the...
The application of low-cost feed additives as an immunostimulant for different species of fish is an emerging research area in aquaculture. The present study aimed at incorporating a byproduct from coffee processing, coffee silverskin, as an immunostimulant in Nile Tilapia. A total of 225 Tilapia fingerlings (each weighing 23.04±0.07) were fed on d...
Growing demands stimulate the intensification of production and create the need for practices that are both economically viable and environmentally sustainable. As European Union banned the use of antibiotics in production in 2003, several alternative treatments have been suggested, including probiotics. The first probiotic study in aquaculture was...
The present study conducted to evaluate the impact of dietary C: N: P ratios on Nile tilapia, growth induces in biofloc system and formation of water biotic communities. Five isonitrogenous, 30% crude protein and isocaloric 23.35 MJ/kg gross energy were formulated. Diets were equal but with variation of mono ammonium phosphate, NH₄H₂PO₄ levels to o...
The present study was aimed at determining the effects of taurine and/or methionine addition to a plant-based diet on growth performance and immunological and antioxidant responses of Persian sturgeon, Acipenser persicus. Four plant-based diets were formulated, including control (C), control+1 g/kg taurine (T), control+2 g/kg methionine (M), and co...
Questions
Questions (3)
Dear all
I am going to use these solutions for IgM activity. However, I could not find the formula for them. So I wonder that if it would be possible for you to help me figure these.
1. Low salt buffer (LSB, 20 mM Tris–HCl, 380 mM NaCl and 0.05% Tween 20, pH 7.3)
2. High salt buffer (HSB, 20 mM Tris–HCl, 500 mM NaCl and 0.1% Tween 20, pH 7.7)
3. Citric acid/sodium acetate buffer
Yours faithfully
I am going to use Real-time PCR for determination of probiotics (or total bacteria) in the gastrointestinal tract of Tilapia. So I wonder that if it would be possible for anyone to recommend the best primer (or method) for this identification.
Thank you.
I am going to add the probiotic in fish diet with the new technique (encapsulate or micro-encapsulate). So could you please give me the protocol for these technique.
Thanks