Hidehiko Sano

Hokkaido University | Hokudai · Graduate School of Dental Medicine

This study evaluated the effect of a 0.5% chloramine T solution on a chemical-cured universal adhesive by comparing the light-cured, one-step, self-etch adhesive for the bonding performance, mechanical properties, and resin–dentin interfacial characteristics. Caries-free human molars were randomly assigned into eight groups based on the bonding sys...

Objectives: To determine the effect of an extra hydrophobic resin layer (EHL) on the bond strength and durability of three different pH one-step universal adhesives (UAs) utilized in self-etch (SE) mode, and to explore whether UAs can be used as a primer in two-step bonding system. Methods: Three different pH UAs were utilized: G-Premio Bond (GP...

The aim of direct pulp capping (DPC) is to promote pulp healing and mineralized tissue barrier formation by placing a dental biomaterial directly over the exposed pulp. Successful application of this approach avoids the need for further and more extensive treatment. In order to ensure a complete pulp healing with the placement of restorative materi...

Several desensitizers routinely used clinically for dentin hypersensitivity are expected to inhibit demineralization. This study aimed to evaluate the effectiveness of sealing materials in inhibiting demineralization and increasing fluorine (F) uptake by acid-treated root surfaces. Five noncarious extracted human teeth were used to produce specimen...

Currently, dental adhesives can be divided into two systems; a smear layer-removal approach with etch-and-rinse adhesives or a smear layer-modified approach with self-etching adhesives. After phosphoric acid etching, the smear layer is completely removed. More attention is, however, required when using self-etching adhesives. The smear layer is par...

Background/purpose The most widely utilized irrigation solution in endodontic therapy is sodium hypochlorite (NaOCl). The purpose of this study was to assess the effects of NaOCl on the bond strength of four universal adhesives and one two-step self-etch adhesive to pulp chamber dentin. Materials and methods One hundred sixteen extracted sound hum...

The purpose of this study was to evaluate the microtensile bond strength (µTBS) and Knoop hardness number (KHN) of a novel experimental light-cured resin cement (HL). Eighteen flat dentin surfaces of human molars were polished using #600 SiC paper and bonded to CAD/CAM resin blocks with the respective resin cements and composites: HL, Panavia V5 (P...

This study aims to assess the color stability, water sorption, and solubility of 11 resin composites as commercially available dental products. Twenty samples (10 mm in diameter and 2 mm in thickness) of each material were fabricated using a customized silicone mold, followed by immersion in each of curry, coffee, wine, and distilled water for 28 d...

This study aimed to evaluate the shear bond strength (SBS) of four bonding agents used to bond metal brackets to zirconia under different storage conditions. Four bonding agents were used [FLC: (Fuji ORTHO LC), XT: (TransbondTM XT), RUC-SBU: (Rely XTM Ultimate Clicker Adhesive Resin Cement+Single Bond Universal), and RUC-GBU: (Rely XTM Ultimate Cli...

Teledentistry is an online dental care service that allows patients and dentists to meet in real time, safely, without being at the same location. During the COVID-19 pandemic, real-time videoconferencing has gained popularity in the field of teledentistry, with numerous benefits for both patients and dentists. Online consultations can minimize cos...

Cetylpyridinium chloride (CPC), a quaternary ammonium compound, which is present in mouthwash, is effective against bacteria, fungi, and enveloped viruses. This study was conducted to explore the antiviral effect of CPC on SARS-CoV-2. There are few reports on the effect of CPC against wild-type SARS-CoV-2 at low concentrations such as 0.001%–0.005%...

Objective To evaluate the effect of a time delay before the light activation of resin composite on the microtensile bond strength (µTBS) of self-etch adhesives under dynamic simulated pulpal pressure. Materials and methods One hundred twenty crown segments were prepared from human third molars. Dentin surfaces were prepared with coarse diamond bur...

Purpose: To evaluate whether varying thicknesses of universal adhesives utilizing the additional coating strategy would affect their microtensile bond strength (µTBS) to dentin, hardness, and elastic modulus (mechanical properties). Materials and methods: Ninety-nine human maxillary premolars were cut to expose coronal dentin, ground with regula...

Objectives: To evaluate the survival probability of a recently marketed universal adhesive bonded to dentin in both self-etch (SE) and, etch-and-rinse (ER) mode, compared to its predecessor adhesive based on conventional (C-μTBST) and, double-sided microtensile bond strength test (D-μTBST) and, adhesive thickness. Methods: Occlusal dentin of 12 sou...

Background: This study aimed to evaluate the color matching, light transmittance, and reflectance characteristics of the novel flowable resin composite OCF-001 (OCF). Methods: Fifty-four resin composite molds were made with simulated class I cavities of A2, A3, and A4 shades by filling the rubber mold interspace with Estelite Sigma Quick (ESQ),...

Experimental adhesive, universal adhesive, HEMA concentration, microtensile bond strength

Purpose: To morphologically evaluate the interface between a conventional glass-ionomer cement (GIC) and dentin one day after placement, as well as the changes at the interface after one year of aging/functioning in monkey teeth. Materials and methods: On the buccal surfaces of seven intact teeth in each of two monkeys, shallow class V cavities...

Despite recent advances in bonding restorations, which are the basis of restorative dentistry, secondary caries are still able to form. Previously, a novel fluoride-containing zinc and copper (ZCF) nanocomposite was introduced to prevent the formation of caries due to its antibacterial activity. In this study, we studied the impact of ZCF nanoparti...

Enamel and dentine bonding can be accomplished by either removing the smear layer before applying the adhesive (etch-and-rinse approach, E) or by using an adhesive that can dissolve the smear layer and penetrate across it to achieve bonding to the hard dental tissue underneath (self-etch approach, S) 1. Some clinical evidence suggests that in the h...

This study evaluated the antibacterial activity of colloidal platinum nanoparticles (CPNs) toward Streptococcus mutans (S. mutans) viability. S. mutans 109c was treated with water and three CPN solutions at 37°C for 24 h (i.e., control, PAA-Pt, C-Pt, C-CyD-Pt). Dilution series (10-1-10-5) were prepared using brain heart infusion (BHI) broth for all...

Background/purpose Glass-ionomer cement (GIC) is bioactive and independent. Bioactivity, which is a big trend in restorative dentistry. When they actively stimulate with microbiological species besides their primary function of restoring tooth structure then restorative materials should called “bioactive” materials. The purpose of this study was to...

Objectives: This study evaluated the effects of different smear layer preparations on the dentin permeability and microtensile bond strength (µTBS) of 2 self-etching adhesives (Clearfil SE Bond [CSE] and Clearfil Tri-S Bond Universal [CTS]) under dynamic pulpal pressure. Materials and methods: Human third molars were cut into crown segments. The...

This study aimed to evaluate the pulpal responses of monkey’s pulp after direct pulp capping (DPC) with the novel mineral trioxide aggregate containing phosphorylated pullulan-based material (MTAPPL). Seventy-two teeth were randomly divided into four groups: MTAPPL; Nex-Cem MTA (NX); TheraCal LC (TH); and Dycal (DY). Histopathological changes in th...

Objective: To evaluate adhesives' enamel bonding performance utilising the traditional microtensile bond strength test (µTBST) and a new double-sided microtensile bond strength test (DµTBST) to assess the suitability of the latter. Methods: A 'tug-of-war' direct encounter design was employed to compare the enamel bond strengths of two universal...

Purpose: This study evaluated whether active application of self-etching adhesives would influence their microtensile bond strength (µTBS) to dentin cut with burs of different grit sizes. Materials and methods: Eighty-four human premolars were divided into 12 groups according to 1) two dentin surface preparations with either superfine- or regul...

Aim To evaluate the dental pulp responses to a novel mineral trioxide aggregate containing phosphorylated pullulan (MTAPPL) in rats after direct pulp capping. Methods Ninety-six cavities were prepared in maxillary first molars of 56 male Wister rats. The dental pulps were intentionally exposed and randomly divided into four groups according to the...

This study evaluated an experimental two-step self-etch adhesive (BZF-29, BZF) by comparing it with a reference two-step self-etch adhesive (Clearfil Megabond 2, MB) and a universal adhesive (G-Premio Bond, GP) for microtensile bond strength (μTBS) and resin-dentin interfacial characteristics. Twenty-four human third molars were used for the μTBS t...

The complex, dynamic, and hydrated microstructures of human dentin serve as the major determinant for the restorative performance of biomaterials. This study aimed to evaluate the mechanical properties of human dentin under different hydration conditions. The occlusal dentin of five third molars was exposed and cut into 1 mm2 dentin slabs. The slab...

Objective: To evaluate the shade matching ability of a novel supra-nano filled esthetic resin composite employing structural color technology using simplified simulated clinical cavities. Filler morphology and light transmittance characteristics were also evaluated. Materials and Methods: One-hundred and twenty frames of resin composite were built...

This study evaluated the effects of remaining dentin thickness (RDT), different smear layers, and aging on the microtensile bond strength (μTBS) of universal adhesives to dentin when applied in self-etch mode. Ninety-six human third molars were randomly allocated to 12 groups (n=8) based on adhesives: ClearfilTM SE Bond 2 (SE, control), ClearfilTM...

Keywords: Microtensile bond strength test; Adhesion; Caries-affected dentin; Sclerotic dentin; Minimal intervention; Resin composite. Summary: Microtensile bond strength (TBS) test was introduced in 1994. Since then, it has been utilized profoundly across many bond strength testing laboratories, making it currently one of the most standard and vers...

Objective To evaluate the shade matching ability of a novel supra‐nano filled esthetic resin composite employing structural color technology using simplified simulated clinical cavities. Filler morphology and light transmittance characteristics were also evaluated. Materials and Methods One‐hundred and twenty frames of resin composite were built i...

Objective The purpose of this study was to determine the bond stability and the change in interfacial ultra-structure of a conventional glass-ionomer cement bonded to dentin, with and without pre-treatment using a polyalkenoic acid conditioner. Methods The occlusal dentin surfaces of six teeth were ground flat. Glass-ionomer cement was bonded to t...

This study aimed to evaluate the bonding performance of a new one-step self-etching adhesive system containing a novel hydrophilic amide monomer. Clearfil Universal Bond Quick (CUB) and Clearfil Megabond 2 (CMB) were used as the one-step and two-step adhesive systems, respectively. Flat dentin surfaces of human premolars were exposed using #600 SiC...

The purpose of this study was to evaluate the air-blowing temperature and water storage time on the micro-tensile bond strength (μTBS) of five universal adhesive systems to dentin. The bond strength with two different air-blowing temperatures (60±2ºC and 23±2ºC) was measured after water storage at 37ºC for 24 h and 100 days respectively. The fractu...

Using transmission electron microscopy (TEM), this study investigated the characteristics of smear layers after preparation of dentin surfaces using different methods. In addition, this study analyzed, using scanning electron microscopy (SEM), the interactions of smear layers with the various acidic agents used subsequently to treat the dentin surf...

In this study, we investigated water-dispersible surface modification for size- and shape-controlled fullerene nanoparticles (C 60 P) based on a condensation reaction with di-amino alkane. This modification provided for water dispersibility of C 60 P and the capability for secondary modification as well. The resultant C 60 P particles have several...

Purpose: To evaluate the effects of double application of resin cement primers to dentin prepared with different diamond burs on the cement-dentin bond strengths. Materials and methods: Sixty flat dentin surfaces were prepared and divided into 12 groups (n = 5) according to three experimental factors: 1. resin cements: RelyX Ultimate (RU), Multi...

The removal or modification of smear layers that cover the dentin is critical to allow the penetration of adhesive molecules and to ensure a strong bond between resin and dentin. Aiming to establish a model for clinically-relevant dentin-bond testing, we evaluated the effects of smear layers created by abrasives having similar coarseness (180-grit...

This study aimed to evaluate the ability of chewing gum containing sodium metaphosphate (SMP) to remove coffee stains from enamel in situ. This was a double-blind (subjects, evaluators), parallel-group, crossover, randomized clinical trial with 30 healthy adult volunteers. Each participant held an appliance with a hydroxyapatite (HA) pellet on the...

Glass-ionomer cement is primarily used for its ability to prevent caries due to sustained release of fluoride. In recent years, it has been proven as a carrier of various kinds of ions other than fluoride, and attention is paid to its property as a bioactive material. However, the release of ions from the material is not stable, and therefore, the...

Dental materials that are antimicrobial and acid-resistant can inhibit bacterial colonization and demineralization, thereby preventing caries. Zinc and copper are well-known for their antibacterial effect, as is nanostructured ZnO–CuO composite. Minerals such as fluorine and calcium, can remineralize and demineralize teeth. Therefore, we developed...

Objectives To evaluate the effects of air-blowing time and storage time on microtensile bond strength (μTBS) of universal adhesives to dentin. Materials and methods Ninety flat dentin surfaces from extracted human third molars were bonded with three universal adhesives (Clearfil Universal Bond-CU; G-Premio Bond-GP; Scotchbond Universal Adhesives-S...

This study evaluated the effects of dehydration on the mechanical properties of adhesive resins and dentin, and on the microtensile bond strength (µTBS) of adhesives. Third molars were randomly bonded with Clearfil Mega Bond (MB) or Clearfil SE Bond 2 (SE). After water-storage (37°C; 24 h), µTBS was obtained in ‘wet’ (tested after 5 min of removal...

Purpose: To evaluate the effects of double application of self-etch adhesives on their microtensile bond strength (μTBS) to dentin, as well as the hardness (H) of resin-dentin interfacial structures. Materials and Methods: Midcoronal dentin surfaces (n = 45) were polished with 180-grit SiC paper and randomly allocated to 9 groups based on three adh...

Purpose: To evaluate the effects of double application of self-etch adhesives on their microtensile bond strength (μTBS) to dentin, as well as the hardness (H) of resin-dentin interfacial structures. Materials and methods: Midcoronal dentin surfaces (n = 45) were polished with 180-grit SiC paper and randomly allocated to 9 groups based on three...

Purpose: To evaluate the potential of an additional application of two novel hydrophobic experimental adhesive resins with or without bioactive zinc fluoride glass to promote the bond strength of a one-step self-etch universal adhesive. Materials and methods: Three self-etch universal adhesives, G-Premio Bond (GPB), Scotchbond Universal (SBU) an...

In this study, we investigated in cytocompativility and controlled release behavior of a model drug from nanoporous silica microparticles (NPSM). When Mouse osteoblastic cells (MC3T3-E1) were exposed to NPMSs, they indicated excellent cell viability. From NPSM contained in dental glass ionomer cement (NPMS-GIC), they can release cationic model drug...

The purpose of this study was to evaluate the effect of surface roughness and smear layer on the micro-shear bond strength (µSBS) of two self-etching adhesives, Clearfil SE Bond (SE) and Clearfil Tri-S Bond (S3). Flat dentin surfaces were prepared with SiC papers (600-, 180- and 120-grit) and diamond burs (extra fine, medium and coarse). They were...

Tooth-bound (KOH-insoluble) fluoride contributes to caries prevention because of its low acid solubility and long retention within tooth structure. The purpose of this study was to evaluate tooth-bound fluoride in dentin, taken up from fluoride-containing luting materials, using an in-air [Formula: see text]PIXE/PIGE system. The dentin surface of t...

Purpose: To determine the microtensile bond strength (μTBS) of two 2-step self-etch adhesives (SEAs) to bur-cut and uncut enamel. Materials and methods: The buccal and lingual enamel surfaces of 15 teeth were ground flat ("bur-cut" enamel), while the enamel surface of another set of 15 teeth was solely prophylactically cleaned ("uncut" enamel)....

The purpose of this study was to investigate the effect of remaining dentin thickness (RDT) and long term water storage on dentin bond strength in-vitro. Twenty-seven third molars were randomly divided into 3 groups: Clearfil Bond SE ONE (SE1, Kuraray Noritake Dental, Okayama, Japan), G-Bond plus (GB, GC, Tokyo, Japan) and Clearfil Mega Bond (MB, K...

Objective: This study evaluated the effect of shortened application time on long-term bond strength with universal adhesives. Methods and materials: Three universal adhesives were used: Clearfil Universal Bond (CU, Kuraray Noritake Dental Inc, Tokyo, Japan), Scotchbond Universal Adhesive (SB, 3M ESPE, St Paul, MN, USA) or G-Premio Bond (GP, GC C...

In this study, we investigated the controlled release behavior of a model drug from nanoporous silica microparticles (NPSM) contained in dental glass ionomer cement (GIC). NPSM released model drug molecules gradually in water for 2 weeks when they were contained in GIC. In contrast, GIC-only specimens released the molecule rapidly. To estimate the...

HuR is an RNA-binding protein of the embryonic lethal abnormal vision (ELAV) family, which binds to the AU-rich element (ARE) in the 3'-untranslated region (UTR) of certain mRNAs and is involved in the nucleo-cytoplasmic export and stabilization of ARE-mRNAs. The cytoplasmic relocalization of ARE-mRNAs with several proteins such as HuR and pp32 inc...

To assess the chronological participation of sclerostin and FGF23 in bone metabolism, this study tracked the immunolocalization of sclerostin and FGF23 in the metaphyses of murine long bones from embryonic day 18 (E18) through 1 day after birth, 1 week, 2 weeks, 4 weeks, 8 weeks, and 20 weeks of age. We have selected two regions in the metaphyseal...

Objectives: To evaluate the influence of different air-blowing durations on the micro-tensile bond strength (μTBS) of five current one-step adhesive systems to dentin. Methods: One hundred and five caries-free human molars and five current one-step adhesive systems were used: ABU (All Bond Universal, Bisco, Inc.), CUB (CLEARFIL™ Universal Bond,...

Objective: Bioactive glasses and surface pre-reacted glass-ionomer (sPRG) filler possess cariostatic properties owing to ion release. Many studies investigated potential cariostatic effects; few studies evaluated the surface stability and the structural changes their surfaces undergo in acidic conditions. Methods: The surface resistance against...

Severe periodontal diseases cause root exposure, and there is no established method for the protection of exposed root surfaces. PRG Barrier Coat is a coating material with bioactive prereacted glass ionomer filler (hereafter, filler). However, the material can be detached in some clinical situations. We evaluated the effects of PRG Barrier Coat re...

Purpose: To evaluate a new root coating material containing surface pre-reacted glass-ionomer (S-PRG) filler for remineralization of demineralized dentin. Methods: The dentin was exposed on root surfaces of human third molars and demineralized by immersion in demineralization solution for 4 days. The demineralized dentin surface was divided into...

The purpose of this study was to compare the micro-tensile bond strength (µTBS) of three resin core composites to dentin and to examine the bonded interface of the composites. One experimental TDK-03(TD) and, two commercial, DC core Automix One (DC) and Unifil core EM(UN) were used. Flat dentin surfaces of human molars were exposed using #600 SiC p...

In this study, we investigated the behaviors and cytocompatibility response of human cervical carcinoma (HeLa) cells expose to nano-sized particles. Cultivated cells exposed to titanium oxide and indium oxide nanoparticles remained highly viable. In the presence of copper oxide (CuO); however, the cells became seriously inflamed. To understand the...

The purpose of this study was to evaluate the μTBS (microtensile bond strength) of currently available self-etching adhesives with an experimental self-etch adhesive in normal and caries-affected dentin, using a portable hardness measuring device, in order to standardize dentin Knoop hardness. Normal (ND) and caries-affected dentin (CAD) were obtai...

We assessed the biocompatibility of nano-sized ceramic particles with several cells types. Though these particles have less than 100 nm in diameter, they act as submicron-sized particles in saline by aggregation that was estimated using laser diffraction particle size analysis (LDS). they act as submicro-sized particles in saline by aggregation bas...

The structures and electronic states of alkyl-radical-functionalized C20 fullerenes (denoted by C20–R) have been investigated using density functional theory (DFT). The different alkyl radicals investigated were methyl, ethyl, propyl, and butyl radicals. The DFT calculation indicated that the alkyl radical binds to the carbon atom of C20 in the on-...

Purpose: With the recent advent of many 1-step adhesive systems, clinicians’preferences have also been shifting to these systems in the clinics, although these adhesives are reported to produce lower bond strength compared to 2- or 3-step adhesives. Most of the 1-step adhesives are universal adhesives usable either in a self-etching mode or in a to...

Purpose: Fluoride varnishes are commonly used for the prevention of caries in Western countries. The purpose of this study was to observe the remaining materials after removing the materials being used, and to evaluate the effect of fluoride varnish on inhibition of demineralization and uptake of fluorine on the root dentin surface using an automat...

The aim of this study was to evaluate the buffering capacity of glass-ionomer material in vitro. The null hypothesis tested was that there is no effect of cured glass-ionomer pit-and-fissure sealant (PFS) pastes on the environmental acidity as well as the tooth substrate. For each material, a cured PFS disk and a section of human enamel were simult...

To determine the bond stability and the change in interfacial ultrastructure of a conventional glassionomer cement bonded to dentin, with and without pretreatment using a polyalkenoic acid conditioner. The occlusal dentin surfaces of 10 teeth were ground flat. Glass-ionomer cement was bonded to the surfaces either with or without polyalkenoic acid...