Hesham Salem

• PhD
• Head of Faculty at Deraya University

The simultaneous quantification of elexacaftor, tezacaftor, and ivacaftor in their laboratory-prepared ternary mixture, dosage form, and spiked human plasma has been made possible by the development and validation of a straightforward, accurate, environmentally friendly, and selective thin-layer chromatography (TLC) approach. Using ethyl acetate‒n-...

Food colorant Erythrosine B (EB) is an antiviral xanthene dye with a wide range of uses as a colorant in cosmetics and medications. Its availability, affordability, quick labeling, and high sensitivity make it an excellent choice for spectrofluorometric and spectrophotometric examination of amine-based medications. Two quick and accurate spectropho...

Trifluridine and tipiracil can now be determined simultaneously thanks to a novel, quick, sensitive, selective, environmentally friendly, and extremely sensitive approach. To determine the medications under study, the first derivative synchronous spectrofluorimetric technique was used. Trifluridine and tipiracil's first derivative amplitudes have b...

The proposed study introduces a rapid, sensitive, and simple synchronous spectrofluorimetric technique for simultaneous quantification of relebactam, cilastatin, and imipenem in marketed pharmaceutical forms and biological fluids. Using synchronous fluorescence spectroscopy at Δ λ = 110 nm, cilastatin was detected at 360 nm. Fourier Self-Deconvolut...

Using spectroscopy, two quick and sensitive analytical methods based on eosin Y ion pairing were developed and assessed in order to determine capivasertib and futibatinib with high selectivity and sensitivity. The quenching impact of futibatinib or capivasertib on the eosin Y's fluorescence at a pH 3.8 and 3.3 for futibatinib and capivasertib, resp...

Topical tapinarof is used to treat plaque psoriasis (a skin disease in which red and scaly patches form are appeared on some areas of the body). The goal of the current research is to establish a facile and rapid fluorimetric technique for tapinarof analysis. The approach relied on the reaction between the drug and zinc ion through metal complexati...

Pemetrexed is a chemotherapeutic medicine, under the trade name Alimta. Malignant pleural mesothelioma patients. Its application in lung cancer has been studied. Here in, a second spectrofluorimetric method was advanced for quantifying of Pemetrexed in pharmaceutical formulation and spiked human plasma. That method depends on fluorescence derivatiz...

The goal of the current research was to establish a quick and practical fluorometric technique for trifluridine analysis. The approach relied on the drug's complex formation with the zinc ion to produce a high‐fluorescence product. The fluorescence was further enhanced by adding sodium dodecyl sulfate, and it was observed at 450 nm following excita...

A spectrofluorimetric approach that is sensitive, simple, validated, and cost-effective has been proposed for the estimation of amlodipine (AML) and perindopril (PER) in their bulk powders, pharmaceutical formulations, and spiked human plasma. The recommended approach utilized the quantitative quenching effect of the two cited drugs on the fluoresc...

Darolutamide is an oral nonsteroidal androgen receptor antagonist used to delay the process of prostate cancer to metastatic disease and to increase the quality of life for people with advanced prostate cancer. Here, a second spectrofluorimetric method was advanced for quantifying Darolutamide in pharmaceutical formulation and spiked human plasma....

Oral phosphodiesterase inhibitors have emerged as a game changer for the treatment of erectile dysfunction (ED) since attaining FDA approval for its first member, sildenafil, in 1998. Topical penile therapy could be a viable replacement for oral medication that would transform the treatment of ED for many decades to come. This innovative idea could...

An inexpensive, simple, sensitive and validated approach is developed for estimation of fingolimod through production of colored charge transfer complexes of fingolimod with different electron acceptor reagents, including a reaction of fingolimod as n-donor with 7,7,8,8-tetracyanoquinodimethane, tetrachloro 1,4-benzoquinone and tetracyanoethylene a...

Bevacizumab (BVC) is commonly co-administered with 5-fluorouracil (FLO) as a highly successful treatment for colon cancer. In this study, two spectrofluorimetric techniques were combined to provide an approach for concurrent quantification of BVC and FLO that is very sensitive, quick, easy and accurate. The inclusion of Tween-80 micellar system inc...

A recent antineoplastic medication is pemetrexed, this medicine is now being developed and produced on a large scale, thus approaches for quality control are urgently needed. Spectrofluorimetric guidelines for the simple estimation of pemetrexed were validated. Pemetrexed's assay was depends on observations of its native fluorescence at 275/450 nm...

A simple, new, green, and sensitive approach was established and validated for assay of the recently approved antineoplastic medication; darolutamide (DAR) in its authentic form, pharmaceutical formulation, and biological fluids fluorimetrically. This experiment relied on the native fluorescence of the cited drug and detects the ideal solvent utili...

A spectrofluorimetric approach, that is sensitive, simple, validated, and cost-effective has been proposed for the estimation of amlodipine (AML) and perindopril (PER) in their bulk powders, pharmaceutical formulations and spiked human plasma. The recommended approach utilized the quantitative quenching impact of the two cited drugs on the fluoresc...

Finasteride and tadalafil are combined in a pharmaceutical capsules called Entadfi™, that has received FAD approval. It was indicated for the management of male benign prostatic hyperplasia-related urinary tract issues. In the current study, finasteride and tadalafil concentrations in raw form, laboratory prepared mixtures, pharmaceutical preparati...

A new, simple hight performance thin layer chromatography (HPTLC)-Spectrodensitometric strategy was created and approved for the synchronous estimation of four antibacterial specialists: ceftazidime (CEF), tazobactam (TAZ), tobramycin (TOB) and sulbactam (SUL). The four compounds were separated on TLC aluminum plates covered with silica gel 60 F254...

Pharmaceutical product quality control (QC) needs for quick, sensitive, and economical procedures in order to deliver high throughput at low cost, which is the key factor taken into account by such economic facilities. To lessen the risky effects of research laboratories, researchers must take into account the ecological impacts. Anti-inflammatory,...

For quantitation of amlodipine (AML) and perindopril (PER) in their authentic, pharmaceutical formulations and spiked human plasma, a simple, sensitive, validated and inexpensive spectrofluorimetric method has been developed. The proposed method is developed to be based on quantitative quenching effect of two antihypertensive drugs on Eosin Y's nat...

A new, simple, green and highly sensitive method has been established for quantification of recently approved antineoplastic drug Sotorasib (SOTO) in its bulk, tablets and biological fluids. The method depends on measuring the intrinsic fluorescence of the studied drug at wavelengths 350/410 nm at the optimal conditions. Validation of this method w...

A recent antineoplastic medication is pemetrexed, this medicine is now being developed and produced on a large scale, thus approaches for quality control are urgently needed. Spectrofluorimetric guidelines for the simple estimation of pemetrexed were validated. Pemetrexed's assay was depends on observations of its native fluorescence at 275/450 nm...

Bevacizumab (BVC) is commonly co-administered with 5-fluorouracil (FLO) as a highly successful treatment for colon cancer. In this study, two spectrofluorimetric techniques were combined to provide an approach for concurrent quantification of BVC and FLO that is very sensitive, quick, easy and accurate. The inclusion of Tween-80 micellar system inc...

An inexpensive, simple, sensitive and validated approach is developed for estimation of fingolimod through production of colored charge transfer complexes of fingolimod with different electron acceptor reagents, including a reaction of fingolimod as n-donor with 7,7,8,8-tetracyanoquinodimethane, tetrachloro 1,4-benzoquinone and tetracyanoethylene a...

A simple, new, green, and sensitive approach was established and validated for assay of the recently approved antineoplastic medication; darolutamide (DAR) in its authentic form, pharmaceutical formulation, and biological fluids fluorimetrically. This experiment relied on the native fluorescence of the cited drug and detects the ideal solvent utili...

The combination of olanzapine and samidorphan has just been authorised for the treatment of schizophrenia. The current study created a very accurate, sensitive and selective spectroscopic technique based on the first derivative of synchronous fluorescence for determining olanzapine and samidorphan in their pharmaceutical prescriptions without prior...

Oral, quick response, and on demand, also known as a spontaneous oral treatment for erectile dysfunction, is highly needed by both patients and physicians. Vardenafil is selective (fewer side effects) and more effective in difficult-to-treat conditions than sildenafil. This study aims at fostering the dual objectives of using biomolecules such as a...

Approved, straightforward, fast and delicate spectrofluorimetric strategy has been developed for the estimation of tepotinib (TEPO), sotorasib (SOTO) and darolutamide (DARO) as a new antineoplastic drugs. Spectrofluorimetric strategy was based on quantitative fluorescence quenching of MER at 538 nm after being excited at 350 nm by the addition of c...

Efficient topical drug delivery for phosphodiesterase inhibitors (PDE inhibitors) as a potential alternative to the oral therapy could revolutionize the treatment strategy of erectile dysfunction for decades to come. This work aims at investigating three selected members of PDE 5 inhibitors for potential topical penile therapy through studying pref...

Efficient topical drug delivery for phosphodiesterase inhibitors (PDE inhibitors) as a potential alternative to the oral therapy could revolutionize the treatment strategy of erectile dysfunction for decades to come. This work aims at investigating three selected members of PDE 5 inhibitors for potential topical penile therapy through studying pref...

A dependable, sensitive, basic and cheap spectrofluorimetric approach has been created for test of sulfur-containing drug; ixabepilone in bulk powder, vials and human plasma. The approach depends on the quenching effect of ixabepilone on the fluorescence intensity of acetoxymercuric fluorescene (AMF) reagent at λem of 530 nm and λex of 500 nm. Para...

Beta 2 agonists are well known for their use in the treatment of asthma and COPD however in the last few years new indications of beta 2 agonist appeared like reduction of local fats and treatment of preterm labour which required the formulation of new dosage forms and administration strategies. The new developments require accurate, economic and f...

A spectrofluorimetric approach has been developed and validated for determination of sulfur-containing drug; ixabepilone in raw powder, vials and human plasma. This approach studies the quenching effect of IXA on the fluorescence intensity of acetoxymercuric fluorescein (AMF) reagent at λ em of 530 nm and λ ex of 500 nm. All the parameters that can...

In this think about, assurance of lopinavir and ritonavir down to organic concentration level has been carried out. The assurance is based on expanding the selectivity of the spectrofluorimetric procedure by combining both subordinate and synchronous spectrofluorimetric approaches, which allow effective estimation of lopinavir at 248.8 nm and riton...

Approved, basic and taken a toll successful spectroscopic strategies (spectrophotometric and spectrofluorimetric) were created for the measure of seven cephalosporins to be specific; cefpiramide (I), cefuroxime (II), cefoxitin (III), ceftazidime (IV), cefpirome (V), ceftabiprole (VI) and ceftriaxone (VII). The most guideline of these strategies is...

A comparative study for the validation and advancement of two analytical approaches applied for the simultaneous determination of Mometasone Furoate (MF), Miconazole Nitrate (MIC) and Gentamicin (GM) formulated in Momenta® cream. The first approach was TLC-spectrodensitometric method, which was advanced by separating the three components on TLC alu...

For the treatment of internal and external hemorrhoids, policresulen (POL) and cinchocaine hydrochloride (CIN) are used in combination. Using a new, simple, fast, and economical first-derivative synchronous fluorescence spectroscopic process, both drugs were simultaneously determined and validated. At Δλ 60 nm and with a scanning rate of 600 nm/min...

Saxagliptin is an orally active, highly potent, selective and competitive dipeptidyl peptidase (DPP)-4 inhibitor used in the treatment of type 2 diabetes mellitus at doses of 2.5 or 5 mg once daily. The purpose of this study was to evaluate the bioequivalence and to investigate the pharmacokinetic properties of two formulations containing 5 mg saxa...

A exceedingly touchy resonance Rayleigh scattering (RRS) strategy for the assurance of nilotinib (NILO) was introduced. In the pH 3.4 acetate buffer solution, NILO reacted with erythrosine B to produce an ion-association complex, which increased the RRS intensity of the studied system. The enhanced RRS intensity (ΔI) was linearly proportional to th...

A comparative study for the validation and advancement of two analytical approaches applied for the simultaneous determination of Mometasone Furoate (MF), Miconazole Nitrate (MIC) and Gentamicin (GM) formulated in Momenta ® cream. The first approach was TLC-spectrodensitometric method, which was advanced by separating the three components on TLC al...

A comparative study for the validation of two analytical approaches applied for the simultaneous determination of betamethasone valerate (BV), tolnaftate (TF), gentamicin (GM), and clioquinol (CQ) formulated in mixderm ® cream. The first approach was TLC-spectrodensitometric method, which was advanced by separating the four components on TLC alumin...

A comparative study for the validation of two analytical approaches applied for the simultaneous determination of betamethasone valerate (BV), tolnaftate (TF), gentamicin (GM), and clioquinol (CQ) formulated in mixderm ® cream. The first approach was TLC-spectrodensitometric method, which was advanced by separating the four components on TLC alumin...

This work discuss a simple, rapid, accurate, precise, sensitive, validated and effective cost spectrofluorometric method. The technique was applied for the analysis of fingolimod hydrochloride (FIN) in pure form, capsules, human plasma and urine samples. Formation of binary complex between the suggested amino group of (FIN) with Eosin Y (EOY) is th...

Objective To formulate and assess bucco-adhesive films of propranolol hydrochloride for pediatric use. Methods Different films were formulated adopting mucin, polyvinyl alcohol, chitosan and carbopol. A drug/polymer compatibility study was conducted adopting differential scanning calorimetry and Fourier transform infrared spectroscopy. The prepare...

A validated straightforward and sensitive spectrofluorimetric procedure was developed to assay trifluoperazine hydrochloride, promethazine hydrochloride, and perazine maleate. The procedure was dependent on oxidation of the investigated phenothiazines using a known excess of ammonium cerium sulfate as oxidizing agent and overseeing the fluorescence...

The size of the objects that are constituted within an alleged nano metric measurement of 1 to 100 nm is generally the main feature of nanoscience and nanotechnology. The system of unharness may be an essential method of acknowledging contained drug delivery systems in the formation of nano drug. Nanoparticles may surpass the crucial troubles of tr...

Two simple, rapid and novel sensitive fluorimetric and spectrophotometric methods were investigated for the assay of sildenafil citrate (SIL), dapoxetine (DAP), vardenafil (VAD) and tadalafil (TAD) using gold nanoparticles (Au NPs). On the spectrofluorimetric method, gold nanoparticles were used as a fluorescence probe. The addition of drugs to Au-...

The size of the objects that are constituted within an alleged nano metric measurement of 1 to 100 nm is generally the main feature of nanoscience and nanotechnology. The system of unharness may be an essential method of acknowledging contained drug delivery systems in the formation of nano drug. Nanoparticles may surpass the crucial troubles of tr...

Simple, sensitive and reliable spectro-chemometric, Chemometric and thin layer chromatography (TLC)-densitometric methods were developed for simultaneous determination of binary mixture of timolol (TIM) and travoprost (TRA) without prior separation in their bulk drugs and dosage forms. The speectro-chemometric methods used are derivative spectropho...

Simple, accurate, selective and validated stability indicating UV spectrophotometric univariate and multivariate methods were developed for determination of Aripiprazole in presence of its alkaline and oxidative degradation products. The developed univariate methods; derivative ratio spectra-zero crossing, successive derivatives of ratio spectra an...

Simple, sensitive and reliable spectro-chemometric and high performance liquid chromatography (HPLC) methods were developed for simultaneous determination of binary mixture of etilefrine hydrochloride and chlorpheniramine maleate without prior separation in their bulk drugs and dosage forms. The spectro-chemometric methods used are, derivative spec...

The aim of this work is to evaluate simple, sensitive, effective and validated procedures for the determination of cefotaxime, cefoperazone, ceftazidime and cefadroxil. In this study, the methods based on the ability of the cited drugs to reduce Ag+ ions to silver nanoparticles (Ag-NPs) in the presence of Polyvinyl Pyrrolidone (PVP) as a stabilizin...

TLC-spectrodensitometric method was developed and validated for the simultaneous determination of policresulen (POL) and cinchocaine hydrochloride (CIN). The drugs were separated on TLC aluminum plates coated with silica gel 60 F 254 , using chloroform : methanol : ammonia (9.5:0.6:1.0, v/v/v) as a mobile phase and Dragendorff reagent as spot detec...

The present work was conducted to evaluate a simple effective technique for spectrophotometric determination of two flavonoids namely diosmin (1) and rutin (2) in the isolated fractions obtained from Persicaria salicifolia, authentic and pharmaceutical dosage forms, the method is based on the reduction of Ag+ cations to silver nanoparticles (Ag-NPs...

Two simple and sensitive spectrophotometric and spectrofluorimetric methods for the determination of terbutaline sulfate, fenoterol hydrobromide, etilefrine hydrochloride, isoxsuprine hydrochloride, ethamsylate, doxycycline hyclate have been developed. Both methods were based on the oxidation of the cited drugs with cerium (IV) in acid medium. The...

TLC-spectrodensitometric method was developed and validated for the simultaneous determination of policresulen (POL) and cinchocainehydrochloride (CIN). The method is being separated on TLC aluminum plates coated with silica gel 60 F 254 , using chloroform : methanol : ammonia (9.5:0.6:1.0, v/v/v)as a mobile phase and Dragendorff reagent as spot de...

A simple, selective and sensitive kinetic spectrophotometric method was described for estimation of four phenolic sympathomimetic drugs namely; terbutaline sulfate, fenoterol hydrobromide, isoxsuprine hydrochloride and etilefrine hydrochloride. This method is depended on the oxidation of the phenolic drugs with Folin-Ciocalteu reagent in presence o...

Two simple and sensitive spectrophotometric and spectrofluorimetric methods for the determination of terbutaline sulfate, fenoterol hydrobromide, etilefrine hydrochloride, isoxsuprine hydrochloride, ethamsylate, doxycycline hyclate have been developed. Both methods were based on the oxidation of the cited drugs with cerium (IV) in acid medium. The...

Two simple and sensitive spectrophotometric and spectrofluorimetric methods for the determination of terbutaline sulfate, fenoterol hydrobromide, etilefrine hydrochloride, isoxsuprine hydrochloride, ethamsylate, doxycycline hyclate have been developed. Both methods were based on the oxidation of the cited drugs with cerium (IV) in acid medium. The...

A sensitive and selective high performance liquid chromatographic method was developed and validated for the simultaneous quantification of Aripiprazole and co-administered Clonazepam in spiked human plasma. Aripiprazole, Clonazepam and the internal standard Citalopram were extracted from plasma and analyzed on a reversed-phase C18 column using a m...

Two sensitive and selective polyvinyl chloride (PVC) matrix membrane sensors were developed and investigated for determination of the cationic drug Quetiapine Fumarate (QTF) in pure form, in plasma and in presence of its two related compounds namely Quetiapine N-oxide and Des-ethanol Quetiapine. The two sensors (Ι and ΙΙ) were developed using sodiu...

Four sensitive and precise spectrophotometric methods were developed and validated for the simultaneous determination of metronidazole (MTR) and diiodohydroxyquinoline (DIQ) in theirmixture and in their pharmaceutical formulations. Among the methods adopted were, second-derivative (2D), third-derivative (3D), derivative ratio spectroscopy (1DD) and...

This work presents a comparative study on the development and validation of two analytical techniques applied for the simultaneous determination of hydrocortisone acetate (HCA), fusidic acid (FSA), methyl paraben (MPB), and propyl paraben (PPB) formulated as a topical cream. The first technique was thin-layer chromatography (TLC)-densitometric meth...

Sodium cromoglicate (SCG), antihistaminic agent, and tetryzoline hydrochloride (TZH), a sympathomimetic agent, are formulated together as an ophthalmic preparation. An ultra-performance liquid chromatographic method with UV detection (UPLC-UV) was developed and validated for the quantitative determination of SCG and TZH in rabbit aqueous humor. Due...

This paper presents a comparative study between three sensors developed to determine Citalopram Hydrobromide (CT) in the presence of its alkaline hydrolysis and oxidation induced degradation products using different ion association complexes. Sensor 1 was fabricated using phosphomolybdic acid, Sensor 2 used phosphotungestic acid and sensor 3 used t...

Simple, accurate, precise, sensitive, and validated high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC)-densitometric methods were developed for the simultaneous determination of carbinoxamine (CAR), pholcodine (PHL), and ephedrine (EPH) in antitussive syrup. In method A, reversed-phase (RP)-HPLC analysis was performed...

Novel spectrophotometric methods were applied for the determination of the minor component tetryzoline HCl (TZH) in its ternary mixture with ofloxacin (OFX) and prednisolone acetate (PA) in the ratio of (1:5:7.5), and in its binary mixture with sodium cromoglicate (SCG) in the ratio of (1:80). The novel spectrophotometric methods determined the min...

Simple, sensitive, selective, and precise stability-indicating thin-layer chromatography (TLC)-densitometric and reversed-phase high-performance liquid chromatography (RP-HPLC) methods were developed and validated for the determination of citicoline sodium (CT) in the presence of its alkaline degradation products (CT Deg.) and in pharmaceutical ora...

Five, accurate, precise, and sensitive univariate and multivariate spectrophotometric methods were developed for the simultaneous determination of a ternary mixture containing Trimethoprim (TMP), Sulphamethoxazole (SMZ) and Oxytetracycline (OTC) in waste water samples collected from different cites either production wastewater or livestock wastewat...

Membrane selective electrodes were used to determine tetryzoline hydrochloride (TZH) in pure form, pharmaceutical preparations and in biological fluids. The membrane selective electrodes include construction of water insoluble ion-association complexes. The TZH ion exchangers were formed using tetraphenyl borate (TZH-TPB), phosphomolybdic acid (TZH...

The construction and electrochemical response characteristics of three selective electrodes were investigated using precipitation based technique with phosphotungstate and phosphomolybdate; respectively upon using polyvinyl chloride (PVC) matrix and dioctyl phthalate (DOP) as a plasticizer. The resultant membrane sensors were tinidazole phosphotung...

The construction and electrochemical response characteristics of six Ciprofloxacin-selective electrodes were investigated using precipitation based technique with sodium tetraphenyl borate (TPB), phosphomolybdate (PMA) and phosphotungstate (PTA); respectively upon using polyvinyl chloride (PVC) matrix and dibutyl phthalate (DBP) as a plasticizer. T...

High-performance liquid chromatography (HPLC), ultra-performance liquid chromatography (UPLC) and rapid resolution liquid chromatographic (RRLC) methods have been developed and validated for the separation and quantitation of both or either of two ternary mixtures present in ophthalmic solutions. The first mixture contains chloramphenicol, dexameth...

Two methods were described for the simultaneous determination of ciprofloxacin HCl (CIP) and moxifloxacin HCl (MOX) in their binary mixture present in industrial wastewater. A solid-phase extraction procedure (SPE) based on retention on HLB OASIS cartridges and elution with a mixture of methanol-water in acidic medium was preformed, and then both f...

Objectives The solid-state interactions of fused mixtures nimesulide (ND) with polyethylene glycol (PEG) 4000, urea or mannitol were studied through constructing thaw-melt phase equilibrium diagrams. Methods The solid-state characteristics were investigated using differential scanning calorimetry (DSC) and X-ray diffraction (XRD). Various types of...

This work represents the application of the isosbestic points present in different absorption spectra. Three novel spectrophotometric methods were developed, the first method is the absorption subtraction method (AS) utilizing the isosbestic point in zero-order absorption spectra; the second method is the amplitude modulation method (AM) utilizing...

Simple, accurate, sensitive, selective and validated UV spectrophotometric univariate and multivariate methods were developed for determination of Buflomedil (BFM) in presence of BFM degradation products (BFM Deg). The univariate methods includes mean centering of ratio spectra (MCR), ratio difference spectrophotometric method (RDSM) and H-point st...

A validated simple and sensitive spectrofluorimetric method was developed for the determination of chlorpromazine hydrochloride, promethazine hydrochloride, trifluperazine hydrochloride, thioridazine hydrochloride, perazine maleate and oxomemazine. The method was based on condensation of malonic acid/acetic anhydride (MAA) under the catalytic effec...