Hesham Elhariry

• Dr. rer. nat.
• Professor (Full) at Ain Shams University

Aeromonas hydrophila is the most well known of the six species of Aeromonas, which has been linked to two groups of human diseases: septicemia and gastroenteritis. Reference strain ATCC 7966 and biofilm strains TUB19, TUB20, and TUB21 were investigated for their ability to form biofilm in vitro (after 48 h on polystyrene surface) and on the surface...

Onion bulbs can become contaminated with various molds during the storage period, the most important causal agents being black aspergilli (Aspergillus section Nigri). Taxonomic studies have revealed that this group of Aspergillus contains many species that cannot be reliably identified using standard morphological methods. Therefore, it is necessar...

Drinking water distribution networks are known to harbor microbial biofilms. The aim of the present work is to (i) identify the culturable bacteria presented in the drinking-water distribution network, (ii) investigate the ability of isolated bacteria to form biofilm under some environmental stress conditions and some liminating or removing treatme...

Six different blends of lactic acid bacterial cultures (Lactobacillus plantarum or Pediococcus cerevisiae) combined with Saccharomyces cerevisiae were used as co-culture for preparing sourdoughs. These prefermented sourdoughs were applied for producing pan breads. Acidification power, leavening ability, and microbial counts were conducted at differ...

Oral bacterial pathogens, including Bacillus species, form biofilms that enhance antibiotic resistance, promote bacterial adherence, and maintain structural integrity. The ability of bacteria to form biofilms is directly linked to several oral diseases, including gingivitis, dental caries, periodontitis, periapical periodontitis, and peri-implantit...

The urgent need to address the growing problem of antimicrobial resistance in multidrug-resistant bacteria requires the development of pioneering approaches to treatment. The present study aims to evaluate the antimicrobial potential of the essential oils (EOs) of Moringa oleifera (moringa), Cinnamomum verum (cinnamon), and Nigella sativa (black se...

The increasing demand for natural alternatives to synthetic fungicides has prompted research into natural products like essential oils for postharvest disease management. This study investigated the antifungal, antioxidant, cytotoxic, and genotoxic potential of essential oil mixtures derived from oregano, rosemary, and mint against Penicillium digi...

Valorization of poultry waste is a significant challenge addressed in this study, which aimed to produce cost-effective and sustainable peptones from poultry waste. The isolation process yielded the highly potent proteolytic B.subtilis isolate P6, identified through 16S rRNA gene sequencing to share 94% similarity with the B.subtilis strain KEMET02...

Nanoparticles have gained worldwide attention as a new alternative to chemical control agents due to their special physiochemical properties. The current study focused on the environmentally friendly synthesis of silver nanoparticles (AgNPs) using Lycopersicon esculentum peel. In addition to studying the intrinsic cytotoxic effectiveness of Le-AgNP...

Polyphenolics have been predicted to effectively develop antimicrobial agents for the food industry as food additives and promote human health. This study aims to synthesize pomegranate peel extract (PPE) with silver nanoparticles (AgNPs) against eight foodborne pathogens. Multispectroscopic analysis of UV–vis spectroscopy, Zeta potential, Fourier...

Mosquito control in Egypt depends on applying chemical synthetic pesticides that impact negatively on human health and the environment as well as the development of antibiotic and chemical resistance. This study aims to control the 3rd and 4th instars of Culex pipiens larvae using four bacterial strains. According to Phenotypic and molecular identi...

The emergence of antimicrobial resistance in foodborne bacterial pathogens has raised significant concerns in the food industry. This study explores the antimicrobial potential of biosynthesized silver nanoparticles (AgNPs) derived from Agaricus bisporus (Mushroom) against foodborne bacterial pathogens. The biosynthesized AgNPs were characterized u...

According to their special physio-chemical properties, Nanoparticles have gained worldwide attention as a new bio-alternative for chemical control agents. This investigation aims to the eco-friendly synthesis of nanosilver particles from tomato peel extract(TPE-AgNPs) and evaluates their characteristics and inhibitory activities against pathogenic...

Mosquito control in Egypt depends on applying chemical synthetic pesticides that impact negatively on human health and the environment as well as the development of antibiotic and chemical resistance. This study aims to control the 3rd and 4th instars of Culex pipines larvae using four bacterial strains. According to Phenotypic and molecular identi...

Despite their role in controlling pathogens, the misuse of antibiotics leads to the antibiotic resistance phenomenon. The biosynthesis process of silver nanoparticles from plant extracts has earned wide regard worldwide because of its eco-friendly characteristics. The current study aims to biosynthesize and characterize silver nanoparticles from po...

According to their special physio-chemical properties, Nanoparticles have gained worldwide attention as a new bio-alternative for chemical control agents. This investigation aims to the eco-friendly synthesis of nanosilver particles from tomato peel extract (TPE-AgNPs) and evaluates their characteristics and inhibitory activities against pathogenic...

Biosynthesized silver nanoparticles (AgNPs) attracted broad interest in the food sector due to their excellent antimicrobial activities as a new alternative to harmful chemical disinfectants. This work aims to study characterization parameters, antimicrobial and toxicological characteristics of the biosynthesized silver nanoparticles from Agaricus...

The Hepatic disease all over the world had a more attention towards the prevention methods; planned diet can effective and protective. Therefore, this study was aimed to examine the antioxidant and hepatoprotective role of the dried savory leaves (1 and 2%) and its dried hydroethanolic extract (25 and 50 ppm) against carbon tetrachloride (CCl 4) in...

Background and purpose: Staphylococcus sp. are pathogenic bacteria widely investigated for its high incidence in clinical environments and its ability to form strong biofilms. The biofilm-related infections can resist several antimicrobial agents; therefore, the therapy of these infections may face difficulties. The aim of this study is to identify...

Fungal and mycotoxins contamination of food and poultry feeds can occur at each step along the chain from grain production, storage, and processing. A total of 200 samples comprising of mixed poultry feedstuffs (n = 100) and their ingredients (n = 100) were collected from Riyadh, Alhassa, Qassium, and Jeddah cities in Saudi Arabia. These samples we...

Acrylamide (ACR) is produced during food processing and has been shown to cause health problems. Selenium, especially in its nanoscale, is an important trace element in human nutrition. Chitosan‐stabilized selenium nanoparticles (Ch‐SeNPs) stability and its protective potential against ACR‐induced injury in rats were evaluated. Ch‐SeNPs displayed h...

Diabetic neuropathy is one of the complications of diabetes. This study investigated the possibility of reducing neuropathy of STZ-induced diabetic rats by Terminalia muelleri extract (TE) and comparing the effect of the extract with the therapeutic effect of pioglitazone (PG) drug. The experimental animals were divided into non-diabetic (normal co...

Dihydrofolate (dihydrofolic acid, vitamin B9) is one of the principle reduced forms of folates, which is converted to tetrahydrofolate by dihydrofolate reductase (DHFR). The aim of the present study was to overproduce total folate by inducing accumulation of dihydrofolate (DHF) due to inhibition of DHFR in a thermosensitive mutant (TS) of Lactobaci...

The present study aims at biosynthesizing, characterizing and evaluating the biogenic silver nanoparticles (AgNPs) as antimicrobial and antibiofilm against Kocuria rosea and Kocuria rhizophila. Cellfree supernatant of Proteus mirabilis culture was used for biosynthesizing AgNPs, which confirmed by visualizing color change and X-ray diffraction. Tra...

Alternaria species produce several mycotoxins that are of particular health concern. The natural occurrence of three Alternaria toxins; alternariol (AOH), alternariol methyl ether (AME), and tenuazonic acid (TA) in pomegranate fruit was considered. A. alternata and A. tenuissima were identified by analysis of partial sequence of ITS-region. All stu...

Biofabricated metal nanoparticles is under exploration due to wide biomedical applications and research interest in nanotechnology. Biosynthesis of silver and gold nanoparticles was achieved by reduction of silver nitrate (AgNO3) and chloroauric acid (HAuCl4), respectively, using ginger rhizome (Zingiber officinale) extract. The biosynthesized silv...

Lactobacillus has a well known positive effect on human health. Therefore, this study aimed at applying the antimicrobial and antibiofilm effects of L. pentosus, L. plantarum and L. pentosus HG against 2 common pathogens, Bacillus cereus (food born pathogen) and Pseudomonas aeruginosa (plant pathogen). All lactic acid bacterial strains were identif...

Lactobacillus has a well known positive effect on human health. Therefore, this study aimed at applying the antimicrobial and antibiofilm effects of L. pentosus, L. plantarum and L. pentosus HG against 2 common pathogens, Bacillus cereus (food born pathogen) and Pseudomonas aeruginosa (plant pathogen). All lactic acid bacterial strains were identif...

Fungal endophytes were isolated from twigs of Juniperusprocera (Cupressaceae) collected from Taif region (Saudi Arabia). Twenty-six different taxa were recovered. The overall foliar colonization rate was 36%. A total of 144 isolates were obtained and identified into six distinct operational taxonomic units based on the sequencing of the internal tr...

The present work aimed to determine the bioactive compounds in two rosemary water extracts (RWE1 and RWE2) and to assess their antimicrobial, anti-adhesive and antibiofilm potentials against the food-related Bacillus and Pseudomonas species at concentrations; 4, 8, 12, 16 and 20 mg mL−1. Phenolic compounds and isoflavones in the RWEs were determine...

Recently, new strains of Fasciola demonstrated drug resistance, which increased the need for new drugs or improvement of the present drugs. Nanotechnology is expected to open some new opportunities to fight and prevent diseases using an atomic scale tailoring of materials. The ability to uncover the structure and function of biosystems at the nanos...

The study included collection of water samples (150 samples) from 10 different branches belonging to each water network in Jeddah City (Saudi Arabia). Samples included treated water (30 samples) and tap water in some hospitals (30 samples from cold water tap and 30 samples from hot water tap) and private houses (60 samples). According to results of...

Little is known about the bacterial communities associated with the rose plants inhabiting dry desert ecosystems. The aim of this study was to isolate and characterize endophytic bacteria from different organs of rose plant. Endophytic bacteria were observed in healthy roots, stems, leaves, and flowers of rose plant, with a significantly higher den...

Thirty soil-isolates of Streptomyces were analyzed to determine their antagonism against plant-pathogenic fungi including Fusarium oxysporum, Pythium aristosporum, Colletotrichum gossypii, and Rhizoctonia solani. Seven isolates showed antifungal activity against one or more strain of the tested fungi. Based on the 16S rDNA sequence analysis, these...

This study aimed to determine the mycological profile of the retail date fruits distributed in different markets at Taif, Saudi Arabia. The presence of aflatoxins and ochratoxin A was also measured. Twenty-two fungal species belonging to 12 genera were isolated from 50 different date samples. Aspergillus flavus, A. niger, Penicillium chrysogenum, a...

This work aimed at isolation and identification of patulin producing fungi and to follow the presence of patulin during apple juice processing. Among 34 Penicillium isolates, eight isolates (five from healthy appeared apples and 12 from rot spotted apples) were considered as patulin producers using thin-layer chromatography. These isolates were cla...

A broad range of fungi were isolated from different geographic regions and substrates and identified according to traditional and modern methods. A total of 120 different isolates were assigned to the phyla, Basidiomycota with 8 isolates, Ascomycota with 75 isolates, and “Zygomycota” with 37 isolates. Although morphological characters were able to...

The effect of some food-related and environmental stress conditions (glucose or NaCl, nutrient starvation, heating, UV, agitation and temperature) on the ability of biofilm formation on polystyrene surface by vegetative cells of four Bacillus species was studied. The final concentration of glucose or NaCl in TSB was adjusted within the range releva...

Twelve isolates of Pythium species (P. aphanidermatum, P. deliense, P. ultimum var. ultimum and P. ultimum var. sporangiiferum) from different hosts were compared from morphological, pathological and molecular viewpoints. Minimum, optimum and maximum temperatures of P. aphanidermatum and P. deliense were similar while those of P. ultimum var. ultim...

The thermosensitive phenotype of strain CH31, a derivative of Corynebacterium ammoniagenes ATCC 6872, was allocated by cloning, sequencing, and genetic complementation to a single C→T exchange in the nrdE (nucleotide reduction) gene at nucleotide 1301. Protein modeling indicates the impaired surface hydrophobicity of NrdE(Ts) due to the S434F trans...

Corynebacterium ammoniagenes strain CH31 is thermosensitive due to a mutation in nucleotide reduction (nrd ts). The strain was examined for nucleotide overproduction upon shifting the culture temperature to a range of elevated temperatures. No overproduction of NAD+ was detected in the control maintained at 27°C whereas NAD+ was accumulated extrace...

Flavour enhancers are compounds with no intrinsic flavour of their own, but when are added in low concentrations to appropiate foods improve the palatability of the food distinctly. In addition to the four basic tastes sweet, bitter, salty and sour a fifth taste, ‘umami’, has been recognized in traditional seasonings of the Japanese cuisine with mo...

Cell division of the wild type strain Corynebacterium (formerly Brevibacterium) ammoniagenes ATCC 6872 which requires 1 microM Mn2+ for balanced growth was inhibited by addition of 20 mM hydroxyurea (HU) or 10 mM p-methoxyphenol (MP) to a Mn2+-supplemented fermentation medium at an appropriate time. Scanning electron microscopy (SEM) showed a restr...

Hannover, University, Diss., 2004. Computerdatei im Fernzugriff.