Hervé Dubouchaud

Hervé Dubouchaud
Université Grenoble Alpes · Department of Sport Sciences and Laboratory of Fundamental and Applied Bioenergetics - INSERM 1055

PhD

About

87
Publications
9,425
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Introduction
Professor at the Department of Sport Sciences and Deputy Director of the Laboratory of Fundamental and Applied Bioenergetics - INSERM 1055, Université Grenoble Alpes.
Additional affiliations
February 2021 - August 2021
French National Centre for Scientific Research
Position
  • Researcher
August 2018 - July 2019
The University of Edinburgh
Position
  • Researcher
June 1997 - October 1999
University of California, Berkeley
Position
  • PostDoc Position
Description
  • Exercise Physiology Laboratory

Publications

Publications (87)
Article
Full-text available
The perinatal exposome can modify offspring metabolism and health later in life. Within this concept, maternal exercise during gestation has been reported modifying offspring glucose sensing and homeostasis, while the impact of such exercise during lactation is little-known. We thus aimed at evaluating short- and long-term effects of it on offsprin...
Article
Full-text available
Rationale: Intermittent hypoxia (IH) is one of the main features of sleep-disordered breathing (SDB). Recent findings indicate that hypoxia inducible factor-1 (HIF-1) promotes cardiomyocytes apoptosis during chronic IH, but the mechanisms involved remain to be elucidated. Here, we hypothesize that IH-induced ER stress is associated with mitochondr...
Article
Full-text available
It remains unclear whether sickle cell trait (SCT) should be considered a risk factor during intense physical activity. By triggering the polymerization-sickling-vaso-occlusion cascade, lactate accumulation-associated acidosis in response to high-intensity exercise is believed to be one of the causes of complications. However, our understanding of...
Article
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AMP-activated protein kinase (AMPK) is a key regulator of energy homeostasis under conditions of energy stress. Though heart is one of the most energy requiring organs and depends on a perfect match of energy supply with high and fluctuating energy demand to maintain its contractile performance, the role of AMPK in this organ is still not entirely...
Article
The purpose of this study was to determine whether magnesium L-lactate is responsible for having a beneficial effect on the myocardium and the skeletal muscles and how this substrate acts at the molecular level. Twenty seven young male Wistar rats were supplied with a magnesium L-lactate (L) solution, a magnesium chloride (M) solution and/or water...
Article
Introduction et but de l’étude Dans une étude précédente, nous avons montré qu’en présence d’un cancer et d’une chimiothérapie (dans une situation de tumeur extra-hépatique), il y avait une perturbation du métabolisme mitochondrial hépatique. Par ailleurs, la cachexie est caractérisée par une perturbation du métabolisme énergétique [1]. Notre objec...
Article
This study aimed to test if the non-oxidative energy supply (estimated by the accumulated oxygen deficit) is associated with an index of muscle lactate accumulation during exercise, muscle monocarboxylate transporter content and the lactate removal ability during recovery in well-trained rowers. Seventeen rowers completed a 3-min all-out exercise o...
Preprint
Full-text available
Mitochondrial respiratory states and rates MitoEAGLE Task Group
Article
It is now well established that the intrauterine life environment is of major importance for health during later life. Endurance training during pregnancy is associated with positive metabolic adjustments and beneficial effects on the balance between pro and antioxidant (redox state) in the offspring. Our hypothesis is that these changes could rely...
Article
Full-text available
There is substantial evidence that Reactive Oxygen Species (ROS) play a major part in cell functioning. Although their harmfulness through oxidative stress is well documented, their role in signaling and sensing as an oxidative signal still needs to be investigated. In most cells, the mitochondrial Electron Transport Chain (ETC) is the primary sour...
Article
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Pulmonary hypertension is a co-morbidity, which strongly participates in morbi-mortality in patients with chronic obstructive pulmonary disease (COPD). Recent findings showed that bromodomain-containing proteins, in charge of reading histone acetylation, could be involved in pulmonary arterial hypertension. Our aim was to study the effect of I-BET1...
Article
Accumulating evidence indicates that the MDM2 oncoprotein promotes tumorigenesis beyond its canonical negative effects on the p53 tumor suppressor, but these p53-independent functions remain poorly understood. Here, we show that a fraction of endogenous MDM2 is actively imported in mitochondria to control respiration and mitochondrial dynamics inde...
Article
Key points: Maternal training during gestation enhances offspring body composition and energy substrates handling in early adulthood. Offspring nutrition also plays a role as some beneficial effects of maternal training during gestation disappear after consumption of a high-fat diet. Abstract: Maternal exercise during gestation has been reported...
Article
Full-text available
Some of the Chronic Obstructive Pulmonary Disease (COPD) patients engaged in exercise-based muscle rehabilitation programs are unresponsive. To unravel the respective role of chronic hypoxia and pulmonary inflammation on soleus muscle hypertrophic capacities, we challenged male Wistar rats to repeated lipopolysaccharide instillations, associated or...
Article
Full-text available
Chronic intermittent hypoxia (IH) associated with obstructive sleep apnea (OSA) is a major risk factor for cardiovascular and metabolic diseases (insulin resistance: IR). Autophagy is involved in the pathophysiology of IR and high intensity training (HIT) has recently emerged as a potential therapy. We aimed to confirm IH-induced IR in a tissue-dep...
Article
Full-text available
Background: Exercise and citrulline (CIT) are both regulators of muscle protein metabolism. However, the combination of both has been under-studied yet may have synergistic effects on muscle metabolism and performance. Methods: Three-month-old healthy male rats were randomly assigned to be fed ad libitum for 4 weeks with either a citrulline-enriche...
Article
Key points: Silent mating type information regulation 2 homologue 1 (SIRT1) activity and content increased significantly in overload-induced hypertrophy. SIRT1-mediated signalling through Akt, the endothelial nitric oxide synthase mediated pathway, regulates anabolic process in the hypertrophy of skeletal muscle. The regulation of catabolic signal...
Conference Paper
Introduction et but de l’etude La BPCO associe un etat inflammatoire a des episodes d’hypoxemies ayant de fortes repercussions sur le muscle. Or, la masse musculaire et la survie sont fortement correlees dans la BPCO, conduisant a integrer la rehabilitation a l’effort dans la prise en charge. Cependant, 27 % des patients ne retirent aucun benefice...
Conference Paper
Introduction et but de l’étude Il est aujourd’hui reconnu que l’environnement maternel lors de la gestation affecte non seulement le fœtus mais également la santé future de l’individu [1]. Cependant, très peu d’études ont exploré l’effet de l’exercice maternel pendant la gestation sur l’homéostasie du glucose de la descendance [2]. Le but de notre...
Article
Only few studies explored the effects of maternal exercise during gestation on adult offspring metabolism. We set out to test whether maternal controlled submaximal exercise maintained troughout all gestationnal periods induces persistant metabolic changes in the offspring. We used a model of 15 week-old nulliparous female Wistar rats who exercised...
Article
Full-text available
The purpose of this study was to test if the lactate exchange (γ1) and removal (γ2) abilities during recovery following short all-out supramaximal exercise correlate with the muscle content of MCT1 and MCT4, the two isoforms of the monocarboxylate transporters family involved in lactate and H+ co-transport in skeletal muscle. Eighteen lightweight r...
Article
Introduction et but de l’étude La citrulline et l’exercice sont connus pour être des régulateurs positifs de l’homéostasie musculaire. Cependant, il n’existe à ce jour aucune donnée sur un possible effet synergique. Nous proposons d’évaluer l’effet combiné de la citrulline et de l’exercice sur le métabolisme protéique musculaire et la performance e...
Article
Regular physical activity (PA), along with diet and medication, is one of the three cornerstones of type 2 diabetes therapy. PA decreases insulin resistance through improvements of circulation, lipid oxidation, and decreases in lipotoxicity, low grade inflammation, and oxidative stress associated with ectopic fat deposits and may regulate autophagy...
Article
Full-text available
The Spontaneous Physical Activity (SPA) can be responsible for variations of a lot of physiological parameters at the molecular, cellular, tissue, and systemic levels. It is increasingly recognized that the good understanding of a large part of laboratory experimentations, implies to weight results by this SPA in order to reduce the variability, an...
Data
Figure S2. Representative western‐blots presented in Table 4.
Data
Figure S1. Liver histology : Histological changes in the ZDF rat liver tissues were evaluated using H&E staining.
Article
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The aim of this study was to characterize the early alterations of the liver mitochondrial function in ZDF (fa/fa) rats that develop diabetes compared to that of their lean counterparts ZDF (fa/+). Liver mitochondrial function was examined in 11- and 14-week-old ZDF (fa/fa) and ZDF lean (fa/+) rats. Oxygen consumption, H2O2 release, calcium retenti...
Article
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Decline in skeletal muscle mass and function starts during adulthood. Among causes, modifications of the mitochondrial function could be of major importance. Polyunsaturated fatty (omega-3) acids have been shown to play a role in intracellular functions. We hypothesize that docosahexaenoic acid (DHA) supplementation could improve muscle mitochondri...
Article
Full-text available
Compared to the lean Lou/C rat, the Wistar rat develops increased body weight and abdominal adiposity (IAA). The aim of this study was to compare the functional response to cardiac ischemia/reperfusion of 3-month old Wistar rats with age-matched Lou/C rats, and to explain the differences with regards to mitochondrial hydrogen-peroxide release (mH2O...
Article
Presentation Method: Oral or Poster presentation Please indicate your professional occupation: Other The presenting author fulfills the above conditions and wants to apply for a travel award: Yes I confirm that the presenting author is under the age of 35: Yes Rationale: The impact of maternal environment during fetal life on offspring future healt...
Article
Full-text available
Background There has been accumulating evidence associating diabetes mellitus and cardiovascular dysfunctions. However, most of the studies are focused on the late stages of diabetes and on the function of large arteries. This study aimed at characterizing the effects of the early phase of diabetes mellitus on the cardiac and vascular function with...
Article
Full-text available
To assess the effects of regular physical activity on muscle functional characteristics of carriers of sickle cell trait (SCT), 39 untrained (U) and trained (T) hemoglobin (Hb)AA (CON) and SCT subjects (U-CON, n = 12; U-SCT, n = 8; T-CON, n = 10; and T-SCT, n = 9) performed a graded exercise and a time to exhaustion (T(ex)) test, and were subjected...
Article
Alpha-thalassaemia is known to reduce intra-erythrocyte HbS (sickle haemoglobin) concentration in sickle cell trait (SCT) subjects. Because HbS was shown to increase oxidative stress, the purpose of this study was to assess the effects of the coexistence of α-thalassaemia and SCT on oxidative stress markers and nitric oxide (NO) metabolism after an...
Article
Full-text available
The aim of this study was to analyze the effects of exercise training on oxidative stress in sickle cell trait carriers. Plasma levels of oxidative stress [advanced oxidation protein products (AOPP), protein carbonyl, malondialdehyde (MDA), and nitrotyrosine], antioxidant markers [catalase, glutathione peroxidase (GPX), and superoxide dismutase (SO...
Article
The comparative studies of regulation of oxidative phosphorylation as a function of mitochondrial organization in healthy and cancerous cardiac muscle cells have revealed that the mechanisms of regulation of the energy metabolism depend on the cellular organization (Biochim Biophys Acta, 2010, 1797: 678-97).The aim of this study is to evaluate mito...
Article
Cariporide, an Na/H exchanger inhibitor, is a drug with cardioprotective properties. However, chronic treatment with cariporide may modify the protein phenotype of the cardiomyocytes. Disruption of the equilibrium between a cariporide-modified phenotype and the supply of cariporide could be deleterious. The aim of this study was to test the effects...
Article
A high-fat diet affects liver metabolism, leading to steatosis, a complex disorder related to insulin resistance and mitochondrial alterations. Steatosis is still poorly understood since diverse effects have been reported, depending on the different experimental models used. We hereby report the effects of an 8 week high-fat diet on liver energy me...
Article
With a steadily increasing prevalence, insulin resistance (IR) is a major public health issue. This syndrome is defined as a set of metabolic dysfunctions associated with, or contributing to, a range of serious health problems. These disorders include type 2 diabetes, metabolic syndrome, obesity, and non-alcoholic steatohepatitis (NASH). According...
Article
Full-text available
Aging compromises restoration of the cardiac mechanical function during reperfusion. We hypothesized that this was due to an ampler release of mitochondrial reactive oxygen species (ROS). This study aimed at characterising ex vivo the mitochondrial ROS release during reperfusion in isolated perfused hearts of middle-aged rats. Causes and consequenc...
Article
With a steadily increasing prevalence, insulin resistance (IR) is a major public health issue. This syndrome is defined as a set of metabolic dysfunctions associated with, or contributing to, a range of serious health problems. These disorders include type 2 diabetes, metabolic syndrome, obesity, and non-alcoholic steatohepatitis (NASH). According...
Article
The lactate: its kinetics, metabolism, relationship with performance and controversies The sum of works devoted to the study of lactate, its kinetics during exercise (and/or recovery), its involvement (or not) in muscle fatigue, and its usefulness for training management is impressive. However, the lactate remains the subject of numerous debates, c...
Article
Reçu le 1 er mars 2010 – Accepté le 31 mars 2010 Résumé. La somme de travaux dévolusdévolusà l'´ etude du lactate, de sa cinétique pendant l'exercice (et/ou la récupération), de son implication (ou non) dans la fatigue et de son utilité pour le contrôle de l'entraˆınemententraˆınement est extraordinaire. Pourtant, le lactate reste au centre de nomb...
Article
Full-text available
Besides its well recognized role in lipid and carbohydrate metabolisms, glycerol is involved in the regulation of cellular energy homeostasis via glycerol-3-phosphate, a key metabolite in the translocation of reducing power across the mitochondrial inner membrane with mitochondrial glycerol-3-phosphate dehydrogenase. Here, we report a high rate of...
Article
Full-text available
The first objective was to evaluate the influence of caloric intake on liver mitochondrial properties. The second objective was aimed at determining the impact of increasing fat intake on these properties. Lou/C rats, displaying an inborn low caloric intake and resistant to diet-induced obesity, were compared to Wistar rats fed either ad libitum or...