Hervé Colinet

Université de Rennes 1 | UR1 · Ecosystèmes, Biodiversité, Evolution - UMR CNRS 6553 ECOBIO

Cold acclimation may enhance low temperature flight ability, and salt loading can alter an insects’ cold tolerance by affecting their ability to maintain ion balance in the cold. Presently however, it remains unclear if dietary salt impacts thermal acclimation of flight ability in insects. Here, we examined the effect of a combination of dietary sa...

This chapter describes the main principles of the sterile insect technique (SIT), a pest control method that seeks to gradually reduce insect reproduction by releasing sterilized males in large numbers. We highlight the potential and limitations of the use of SIT and explore various future research avenues. SIT is generally one important component...

The invasive pest Drosophila suzukii is a fruit fly native to Asia that infests a wide variety of fruits. Wild plant species are major reservoirs for D. suzukii populations but their infestation rates vary greatly among geographical areas. We aimed at disentangling the relative roles of macroclimatic, landscape and local factors in the rate of D. s...

Laboratory investigations on terrestrial model-species, typically of temperate origin, have demonstrated that terrestrial ectotherms can cope with daily temperature variations through rapid hardening responses. However, few studies have investigated this ability and its physiological basis in the field. Especially in polar regions, where the tempor...

The spotted wing drosophila (SWD), Drosophila suzukii, is a major invasive fruit pest. There is strong consensus that low temperature is among the main drivers of SWD population distribution, and the invasion success of SWD is also linked to its thermal plasticity. Most studies on ectotherm cold tolerance focus on exposure to a single stressful tem...

Drosophila suzukii is an invasive fruit pest in Europe and America. Females lay eggs into mature fruit that larvae consume causing important losses. Sterile insect technique (SIT) is under development to control this pest. The efficiency of this technique relies on insect quality. However, during the process from mass production to field release, s...

The spotted wing drosophila, Drosophila suzukii , is an invasive pest in Europe and North America. Access to resources may be challenging in late fall, winter and early spring and flies may suffer from food deprivation along with cold stress in these periods. Whereas a plethora of studies have been performed on the overwintering capacity of D. suzu...

Temperate species, contrary to their tropical counterparts, are exposed not only to thermally variable environments with low temperatures but also to long winters. Different selective pressures may have driven divergent physiological adaptations in closely related species with different biogeographic origins. To survive unfavourable winter conditio...

Temperature inducible phenotypic plasticity is a major player in plant responses to climate warming. Functional responses and their role in determining thermal plasticity of plants remain poorly understood. Our objective was to compare trait responses of six populations of Ludwigia peploides resulting from seed from Oceanic climate and from Mediter...

Drosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described from the family Drosophilidae. This deficiency limits our opportunity to use natural host-pathogen combinations in experimental studies, and may bias our understanding of the Drosophila virome. Here we report fourteen DNA...

The control of insect pests often relies on the recurrent application of insecticides. This is the case for the lesser mealworm, Alphitobius diaperinus, an invasive beetle infesting poultry farms. There is evidence that A. diaperinus can develop resistance to several insecticides. Evaluation of such resistance has never been conducted in France, de...

Most species encounter large variations in abiotic conditions along their distribution range. The physiological responses of most terrestrial ectotherms (such as insects and spiders) to clinal gradients of climate, and in particular gradients of temperature, can be the product of both phenotypic plasticity and local adaptation. This study aimed to...

Primary consumers in freshwater ecosystems, such as the zooplankton organism Daphnia magna, are highly affected by cyanobacteria, both as they may use it as a food source but also by cyanobacterial metabolites present in the water. Here, we investigate the impacts of cyanobacterial metabolites focussing on the environmental realistic scenario of th...

Climate change increases average temperatures and the occurrence of extreme weather events, in turn accentuating the risk of organism exposure to temperature stress. When thermal conditions become stressful, the sensitivity of insects toward insecticides can be exacerbated. Likewise, exposure of insects to insecticides can subsequently influence th...

Most species encounter large variations in abiotic conditions along their distribution range. Climate, and in particular temperature, varies along clinal gradients, which determines phenotypic plasticity, local adaptations and associated physiological responses of most terrestrial ectotherms, such as insects and spiders. This study aimed to determi...

Drosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described in association with the Drosophilidae. This has limited the opportunity to use natural host-pathogen combinations in experimental studies, and may have biased our understanding of the Drosophila virome. Here we describe f...

Insects are the largest group of animals. They are capable of surviving in virtually all environments from arid deserts to the freezing permafrost of polar regions. This success is due to their great capacity to tolerate a range of environmental stresses, such as low temperatures. Cold/freezing stress affects many physiological processes in insects...

Larval crowding represents a complex stressful situation arising from inter-individual competition for time- and space-limited resources. The foraging of a large number of individuals may alter chemical and bacterial composition of food, and in turn affect individual's traits. Here we used Drosophila melanogaster to explore these assumptions. First...

Nutrient composition of dietary supply is deeply affecting insects by modulating their physiological processes, resulting in different phenotypes. However, diet could also affect gut microbiota composition, abundance, and roles, with indirect consequences for the host. In this study, we reared Drosophila melanogaster flies on five different diets;...

Cold tolerance is considered an important factor determining geographic distribution of insects. We've previously shown that despite tropical origin, cockroach Gromphadorinha coquereliana is capable of surviving exposures to cold. However, freezing tolerance of this species had not yet been examined. Low temperature is known to alter membranes inte...

Background: Insects have the capacity to adjust their physiological mechanisms during their lifetime to promote cold tolerance and cope with sublethal thermal conditions, a phenomenon referred to as thermal acclimation. The spotted wing drosophila, Drosophila suzukii, is an invasive fruit pest that, like many other species, enhances its thermotole...

Chronic cold exposure is detrimental to chill susceptible insects that may accumulate chill injuries. To cope with deleterious effects of cold temperature, insects employ a variety of physiological strategies and metabolic adjustments, such as production of cryoprotectants, or remodeling of cellular membranes. Cold tolerance is a key element determ...

Drosophila suzukii, an invasive species recently introduced in Europe, lays eggs in thin-skinned fruits and causes huge financial losses to fruit growers. One potential way to control this pest is the sterile insect technique (SIT) which demands a large stock of reproductive females to produce millions of sterile males to be released on demand. Unf...

Drosophila suzukii is an invasive pest causing severe damages to a large panel of cultivated crops. To facilitate its biocontrol with strategies such as sterile or incompatible insect techniques, D. suzukii must be mass‐produced and then stored and transported under low temperature. Prolonged cold exposure induces chill injuries that can be mitigat...

The invasive fruit fly pest, Drosophila suzukii, is a chill susceptible species, yet it is capable of overwintering in rather cold climates, such as North America and North Europe, probably thanks to a high cold tolerance plasticity. Little is known about the mechanisms underlying cold tolerance acquisition in D. suzukii. In this study, we compared...

Insect life is directly depending on environment temperature. Low temperatures can alter cellular homeostasis resulting in chill damages and in most extreme cases, lethality. To counteract these deleterious effects, insects can use acclimation-related physiological adjustments. The spotted wing drosophila (SWD) Drosophila suzukii is an invasive pes...

It is now acknowledged that bacteria from gut microbiota deeply interact with their host by altering many physiological traits. Such interplay is likely to consequently affect stress tolerance. Here, we compared cold and heat tolerance of Drosophila melanogaster flies with undisrupted (control (Co)) versus disrupted gut microbiota (dechorionated eg...

Insects exposed to low temperature often have high mortality or exhibit sublethal effects. A growing number of recent studies have shown beneficial effects of exposing insects to recurrent brief warm pulses during low-temperature stress (fluctuating thermal regime, FTR). The physiological underpinnings of the beneficial effects of FTR on cold survi...

The microbiota is increasingly being recognized as having important impacts on many host biological processes. However, evidence of its effects on animal communication and breeding strategy is lacking. In this three-factorial study, we show that females were more willing to mate with related males, with relatedness likely being assessed through the...

Drosophila suzukii , a vinegar fly originated from Southeast Asia, has recently invaded western countries, and it has been recognized as an important threat of a wide variety of several commercial soft fruits. This review summarizes the current information about the biology and dispersal ofD. suzukiiand discusses the current status and prospects of...

Crowding is a complex stress that can affect organisms' physiology, especially through decreased food quality and accessibility. Here, we evaluated the effect of larval density on several biological traits of Drosophila melanogaster An increasing gradient, from 1 to 1000 eggs per milliliter of food, was used to characterize life-history traits vari...

Hallmarks of aging and age-related mortality (actuarial senescence) have received major focus in the published literature, while fewer studies have investigated functional senescence. Age-related decline in the performance of organism is particularly important for organisms exposed to environmental stress, as the range of conditions allowing physio...

The regulatory mechanisms involved in the acquisition of thermal tolerance are unknown in insects. Reversible phosphorylation is a widespread post-translational modification that can rapidly alter proteins function(s). Here, we conducted a large-scale comparative screening of phosphorylation networks in adult Drosophila flies that were cold-acclima...

The spotted wing Drosophila, Drosophila suzukii, is a new pest in Europe and America which causes severe damages, mostly to stone fruit crops. Temperature and humidity are among the most important abiotic factors governing insect development and fitness. In many situations, temperature can become stressful thus compromising survival. The ability to...

Effect plots from GLM on the experiment with pupae exposed to low temperatures under two relative humidity levels The plots show the conditional coefficients (?marginal effects?) of all variables included in the model as well as effects resulting from the interaction terms. The variables are cold exposure temperature, duration, RH and all the inter...

Effect plots from GLM on the experiment with pupae exposed to high temperatures under two relative humidity levels The plots show the conditional coefficients (?marginal effects?) of all variables included in the model as well as effects resulting from the interaction terms. The variables are heat exposure temperature, duration, RH and all the inte...

Temperatures and respective exposure durations used for adult?s cold tolerance assays

Thermal tolerance landscapes for low and high temperatures under two relative humidity (RH) levels Cold tolerance landscapes under low (A) and high (B) relative humidity, and heat tolerance landscapes under low (C) and high (D) relative humidity. Points corresponds to observed values, and surfaces corresponds to GLMs predictions (Binomial GLM, link...

Temperatures and respective exposure durations used for pupae?s cold tolerance assays

Temperatures and respective exposure durations used for pupae?s thermal stress assays under high or low relative humidity

Effect plots from GLM on the experiment with adults exposed to low temperatures The plots show the conditional coefficients (?marginal effects?) of all variables included in the model as well as effects resulting from the interaction terms. The variables are cold exposure temperature, duration, sex (Male vs Female) and all the interactions. The sta...

Effect plots from GLM on the experiment with adults exposed to high temperatures The plots show the conditional coefficients (?marginal effects?) of all variables included in the model as well as effects resulting from the interaction terms. The variables are heat exposure temperature, duration, sex (Male vs Female) and all the interactions. The st...

Effect plots from GLM on the experiment with pupae exposed to high temperatures The plots show the conditional coefficients (?marginal effects?) of all variables included in the model as well as effects resulting from the interaction terms. The variables are heat exposure temperature, duration and all the interactions. The statistical outputs (from...

Temperatures and respective exposure durations used for pupae?s heat tolerance assays

Thermal tolerance landscapes for low and high temperatures Males (A), females (B) and pupal (C) cold tolerance landscapes, and Males (D), females (E) and pupal (F) heat tolerance landscapes. Points are observed values, and surfaces correspond to GLMs predictions (Binomial GLM, link = logit).

Effect plots from GLM on the experiment with pupae exposed to low temperatures The plots show the conditional coefficients (?marginal effects?) of all variables included in the model as well as effects resulting from the interaction terms. The variables are cold exposure temperature, duration, and all the interactions. The statistical outputs (from...

Temperatures and respective exposure durations used for adult?s heat tolerance assays

The spotted wing Drosophila, Drosophila suzukii , is a new pest in Europe and America which causes severe damages to stone fruits crops. Temperature and humidity are among the most important abiotic factors governing insect life. In many situations, temperature can become stressful thus compromising fitness and survival. The ability to cope with th...

The spotted wing Drosophila, Drosophila suzukii , is a new pest in Europe and America which causes severe damages to stone fruits crops. Temperature and humidity are among the most important abiotic factors governing insect life. In many situations, temperature can become stressful thus compromising fitness and survival. The ability to cope with th...

Environmental stress generally disturbs cellular homeostasis. Researchers have hypothesized that chilling injury is linked to a shortage of ATP. However, previous studies conducted on insects exposed to nonfreezing low temperatures presented conflicting results. In this study, we investigated the mitochondrial bioenergetics of Drosophila melanogast...

Temperature directly affects survival, development and reproduction in insects and thereby it is a key environmental driver for geographic distribution and population dynamics. This study aims at testing the survival of Drosophila melanogaster under constant low temperatures (CLTs) (2, 3, 4, and 5°C) vs. fluctuating thermal regimes (FTRs). In the la...

The cryopreservation techniques proposed for embryos of the fruit fly Drosophila melanogaster are not yet ready for practical use. Alternative methods for long-term storage of D. melanogaster strains, although urgently needed, do not exist. Herein, we describe a narrow interval of low temperatures under which the larvae of D. melanogaster can be st...

When exposed to constant low temperatures (CLTs), insects often suffer from cumulative physiological injuries that can severely compromise their fitness and survival. Yet, mortality can be considerably lowered when the cold stress period is interrupted by periodic warm interruption(s), referred to as fluctuating thermal regimes, FTRs. In this study...

Temperature is a structuring parameter of ecosystems that affects almost all the aspects of biology. This is particularly true for ectotherms that cannot regulate their inner body temperature and are thus subjected to the thermal regime of their environment. Yet the temperature fluctuates naturally at different timescales and insects are able to fa...

Stress tolerance generally declines with age as a result of functional senescence. Age-dependent alteration of stress tolerance can also occur in early adult life. In Drosophila melanogaster, evidence of such a decline in young adults has only been reported for thermotolerance. It is not known whether early adult life entails a general stress toler...

Tél : + 33 2 23 23 66 27; Fax : + 33 2 23 23 50 26 RÉSUMÉ.— Exposition à des conditions desséchantes et tolérance croisée à des stress thermiques chez le petit ténébrion Alphitobius diaperinus (Coleoptera : Tenebrionidae).— Le développement et la prolifération rapides des espèces exotiques dans de nouvelles régions peuvent être liés à leur importan...

All climate change scenarios predict an increase in both global temperature means and the magnitude of seasonal and diel temperature variation. The nonlinear relationship between temperature and biological processes means that fluctuating temperatures lead to physiological, life history, and ecological consequences for ectothermic insects that dive...

The impact of nutritional factors on insect's life-history traits such as reproduction and lifespan has been excessively examined; however, nutritional determinant of insect's thermal tolerance has not received a lot of attention. Dietary live yeast represents a prominent source of proteins and amino acids for laboratory-reared drosophilids. In thi...

The twospotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae) is an important agricultural pest. Population dynamics and pest outbreaks highly depend on the overwintering success of the mite specimens; therefore, it is necessary to assess winter survival dynamics of this pest. Seasonal changes in supercooling point (SCP) and acute cold...