Herbert Braselmann

Herbert Braselmann
Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) | HZM · Research Unit Radiation Cytogenetics

Master of Science

About

181
Publications
11,945
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5,075
Citations
Citations since 2016
43 Research Items
1557 Citations
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2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250

Publications

Publications (181)
Article
Full-text available
Background: We present a functional gene association network of the CLIP2 gene, generated by de-novo reconstruction from transcriptomic microarray data. CLIP2 was previously identified as a potential marker for radiation induced papillary thyroid carcinoma (PTC) of young patients in the aftermath of the Chernobyl reactor accident. Considering the...
Article
Full-text available
Background: Prognostic models based on high-dimensional omics data generated from clinical patient samples, such as tumor tissues or biopsies, are increasingly used for prognosis of radio-therapeutic success. The model development process requires two independent discovery and validation data sets. Each of them may contain samples collected in a s...
Article
Purpose: We herein report on changes in gene expression after radiation exposure to iodine-131 from the Chernobyl accident in the Ukrainian-American thyroid cohort and to external gamma ray or internal plutonium exposure in the Mayak Production Association radiation workers. Materials and methods: Taking advantage of access to tissue samples from t...
Article
Full-text available
Carcinoma cells undergo epithelial-mesenchymal transition (EMT); however, contributions of EMT heterogeneity to disease progression remain a matter of debate. Here, we addressed the EMT status of ex vivo cultured circulating and disseminated tumor cells (CTCs/DTCs) in a syngeneic mouse model of metastatic breast cancer (MBC). Epithelial-type CTCs w...
Article
Purpose: HPV-negative head and neck squamous cell carcinoma (HNSCC) associates with unfavorable prognosis while independent prognostic markers remain to be defined. Experimental design: We retrospectively performed miRNA expression profiling. Patients were operated for locally advanced HPV-negative HNSCC and had received radiochemotherapy in eig...
Article
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Because of the increasing application of ionizing radiation in medicine, quantitative data on effects of low-dose radiation are needed to optimize radiation protection, particularly with respect to cataract development. Using mice as mammalian animal model, we applied a single dose of 0, 0.063, 0.125 and 0.5 Gy at 10 weeks of age, determined lens o...
Article
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Breast cancer is the second leading cause of cancer death among women worldwide and besides life‐style, age and genetic risk factors, exposure to ionising radiation is known to increase the risk for breast cancer. Further, DNA copy number alterations (CNAs), which can result from radiation‐induced double‐strand breaks, are frequently occurring in b...
Article
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Activation‐induced cytidine deaminase (AID) is required for the immunoglobulin diversification processes of somatic hypermutation, gene conversion and class‐switch recombination. The targeting of AID's deamination activity is thought to be a combination of cis‐ and trans‐acting elements, but has not been fully elucidated. Deletion analysis of putat...
Article
Ionising radiation is a well-recognised risk factor for the development of breast cancer, however, it is unknown whether radiation-specific molecular oncogenic mechanisms exist. We investigated post-Chernobyl breast cancers from radiation-exposed female clean-up workers and non-exposed controls for molecular changes. Radiation-associated alteration...
Chapter
In integrative radiation systems biology, relationships between variables generated from different molecular levels are investigated. Two approaches to detect correlations in subsets of bivariate continuous data are discussed. The approaches are based on two-component finite Gaussian mixture models and on parametric bootstrap of the null-hypothesis...
Article
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Gene expression time-course experiments allow to study the dynamics of transcriptomic changes in cells exposed to different stimuli. However, most approaches for the reconstruction of gene association networks (GANs) do not propose prior-selection approaches tailored to time-course transcriptome data. Here, we present a workflow for the identificat...
Data
Significantly enriched senescence associated pathways with corresponding differentially expressed genes. Table presents the names of significantly enriched (FDR<0.05) senescence associated pathways with corresponding differentially expressed genes for all treatment conditions. (XLSX)
Data
Reconstructed gene association networks. All obtained gene association networks are provided as R-objects of type igraph. (RDATA)
Data
Lists of differentially expressed genes. Table includes differentially expressed genes identified by spline regression and BETR methods. Additionally, a list of overlapping differentially expressed genes between both methods is included. (XLSX)
Data
Lists of significantly enriched pathways using differentially expressed genes identified by spline regression method. Four lists of significantly enriched pathways correspond to each used treatment condition. Lists include total numbers of known genes in the pathways, numbers of differentially expressed genes that belong to a single pathway (matche...
Data
Lists of 5% of most important genes identified by centrality measures. Lists of 5% highest ranked genes from the reconstructed gene association networks using spline regression and BETR methods. Overlap represents common most important genes identified in networks from compared methods. (XLSX)
Data
Lists of pathways after mapping of 5% highest ranked genes from the reconstructed gene association networks. Lists include names of pathways together with names of mapped most important genes. (XLSX)
Article
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Background Due to lack of a targeted therapy for the triple-negative breast cancer (TNBC) patients, it is important to explore this aggressive breast cancer type in more detail and to establish novel therapeutic approaches. TNBC is defined negative for the protein expression of oestrogen receptor (ER), progesterone receptor (PR) and human epidermal...
Article
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PC4 is an abundant single-strand DNA binding protein that has been implicated in transcription and DNA repair. Here, we show that PC4 is involved in the cellular DNA damage response. To elucidate the role, we used the DT40 chicken B cell model, which produces clustered DNA lesions at Ig loci via the action of activation-induced deaminase. Our resul...
Article
The triple-negative breast cancer (TNBC) is a very aggressive tumor type often occurring in young women and is associated with a bad prognosis for the patients. TNBC lacks established targets for breast cancer therapy, such as the estrogen receptor (ER), progesterone receptor (PR) and the human epidermal growth factor receptor 2 (HER2). Therefore,...
Article
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Histone demethylases have recently gained interest as potential targets in cancer treatment and several histone demethylases have been implicated in the DNA damage response. We investigated the effects of siRNA-mediated depletion of histone demethylase Jarid1A (KDM5A, RBP2), which demethylates transcription activating tri- and dimethylated lysine 4...
Data
Depletion of Jarid1A does not lead to strong induction of p21. Expression of p21 after depletion of Jarid1A in HeLa, MCF-7 and U2OS cells, 72 h after transfection with scr or Jarid1A siRNA. A representative sample and its quantitative evaluation are shown. Cont = untransfected control. (TIF)
Data
Depletion of Jarid1A does not affect anti-correlation of γH2AX and H3K4me3 or active RNA Pol II, respectively, after ion irradiation. HeLa cell transfected with scr or Jarid1A A1+A3 siRNAs were subject to ion microirradiation with single carbon ions applied in line patterns (lateral distance between single ion hits 1 μm, distance between “lines” 5...
Data
Early formation of γH2AX foci is not affected by depletion of Jarid1A. 72 h after transfection with scr or Jarid1A A1+A3 siRNA, HeLa cells were irradiated in a small angle configuration with 55 MeV carbon ions and fixed after 5 min (A) and 2 min (B), or irradiated with 5 Gy X-rays and fixed after 15 min (C). Indirect immunofluorescence was performe...
Data
Depletion of Jarid1A enhances radiosensitivity. Colony formation experiment with Hela cells transfected with JaridA1 siRNAs A1 or A3 or a combination thereof, as well as untransfected controls and cells transfected with scr siRNA. Cells were irradiated 72 h after siRNA transfection with 0 Gy, 2 Gy, 5 Gy or 10 Gy X-rays. Cells were incubated for 10...
Data
Background and residual 53BP1 and Rad51 foci are not affected by depletion of Jarid1A. Mean background frequency and number of residual 53BP1 and Rad51 foci (+/- SEM) were determined in at least 20 cells after 5 Gy X-irradiation. Cells were fixed before irradiation or at 24 h and 48 h after irradiation and indirect immunofluorescence was performed....
Data
Efficient down-regulation of Jarid1A is associated with a global increase of H3K4me3. (A) Decreased levels of Jarid1A in whole cell protein extracts of HeLa, MCF-7 and U2OS cells after siRNA transfection. Western blot images show levels of Jarid1A in untransfected cells (cont), in cells transfected with scrambled siRNA (scr) and in cells transfecte...
Data
MNase accessibility is not affected by Jarid1A depletion. Analysis of chromatin accessibility by MNase digestion of isolated nuclei. After different incubation periods with 0.5 u MNase, comparable amounts of partially digested DNA were loaded onto an agarose gel. The emerging ladder of mono- and oligonucleosomes is comparable in both samples indica...
Data
Comparison of relative band intensities corresponding to Jarid1A and Jarid1B proteins. Band intensities were normalized with respect to Tubulin loading control. Intensities in HeLa cells were set as 100%. All bands to be compared were on the same blot. Data are from 2 Western Blots. (DOCX)
Article
Full-text available
Colony formation assay is the gold standard to determine cell reproductive death after treatment with ionizing radiation, applied for different cell lines or in combination with other treatment modalities. Associated linear-quadratic cell survival curves can be calculated with different methods. For easy code exchange and methodological standardisa...
Article
Methods: We evaluated uPAR and its interaction partners (uPAR-interactome) by immunohistochemistry (IHC) in tissue microarrays (TMA) prepared from ~300 TNBC tumor tissue specimens and in the TNBC model cell lines BT549 and MDA-MB231. Protein expression of 25 different factors constituting the uPAR-interactome was evaluated by IHC; several of the u...
Article
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One of the major consequences of the 1986 Chernobyl reactor accident was a dramatic increase in papillary thyroid carcinoma (PTC) incidence, predominantly in patients exposed to the radioiodine fallout at young age. The present study is the first on genomic copy number alterations (CNAs) of PTCs of the Ukrainian American cohort (UkrAm) generated by...
Article
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The prediction of therapy response in head and neck squamous cell cancer (HNSCC) requires biomarkers, which are also a prerequisite for personalised therapy concepts. The current study aimed to identify therapy-responsive microRNAs (miRNAs) in the circulation that can serve as minimally invasive prognostic markers for HNSCC patients undergoing radi...
Article
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Background: MiR-221/-222 are frequently overexpressed in breast cancer and are associated with increased malignancy. The specific modification of microRNAs (miRNAs) expression could be a promising strategy in breast cancer therapy, leading to the suppression of tumourigenic processes in tumour cells. Methods: MiR-221/-222 expressions were analysed...
Article
Introduction: The expression of miR-221 and -222 is frequently altered in invasive breast cancer and in breast cancer cell lines. We aimed to determine the prognostic and/or therapeutic impact of miR-221/222 expression and its association with tumor progression and metastasizing processes. Experimental procedures: We investigated the expression of...
Article
Chemotherapeutic drugs kill cancer cells, but it is unclear why this happens in responding patients but not in non-responders. Proteomic profiles of patients with oesophageal adenocarcinoma may be helpful in predicting response and selecting more effective treatment strategies. In this study, pre-therapeutic oesophageal adenocarcinoma biopsies were...
Article
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The possibility of a large-scale acute radiation exposure necessitates the development of new methods that could provide rapid individual dose estimates with high sample throughput. The focus of the study was an intercomparison of laboratories' dose-assessment performances using gene expression assays. Lithium-heparinized whole blood from one healt...
Article
The focus of the study is an intercomparison of laboratories' dose-assessment performances using the γ-H2AX foci assay as a diagnostic triage tool for rapid individual radiation dose assessment. Homogenously X-irradiated (240 kVp, 1 Gy/min) blood samples for establishing calibration data (0.25-4 Gy) as well as blinded test samples (0.1-6.4 Gy) were...
Article
The study design and obtained results represent an intercomparison of various laboratories performing dose assessment using the dicentric chromosome analysis (DCA) as a diagnostic triage tool for individual radiation dose assessment. Homogenously X-irradiated (240 kVp, 1 Gy/min) blood samples for establishing calibration data (0.25-5 Gy) as well as...
Article
The focus of the study is an intercomparison of laboratories' dose-assessment performances using the cytokinesis-block micronucleus (CBMN) assay as a diagnostic triage tool for individual radiation dose assessment. Homogenously X-irradiated (240 kVp, 1 Gy/min) blood samples for establishing calibration data (0.25-5 Gy) as well as blind samples (0.1...
Article
Rapid biodosimetry tools are required to assist with triage in the case of a large-scale radiation incident. Here, we aimed to determine the dose-assessment accuracy of the well-established dicentric chromosome assay (DCA) and cytokinesis-block micronucleus assay (CBMN) in comparison to the emerging γ-H2AX foci and gene expression assays for triage...
Article
Full-text available
Background CRIP1 (cysteine-rich intestinal protein 1) has been found in several tumor types, its prognostic impact and its role in cellular processes, particularly in breast cancer, are still unclear. Methods To elucidate the prognostic impact of CRIP1, we analyzed tissues from 113 primary invasive ductal breast carcinomas using immunohistochemist...
Article
Full-text available
Breast cancer is the most common malignancy in women of the Western world. One prominent feature of breast cancer is the co- and overexpression of human epidermal growth factor receptor 2 (HER2) and protein tyrosine kinase 6 (PTK6). According to the current clinical cancer therapy guidelines, HER2-overexpressing tumors are routinely treated with tr...
Article
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Background There is evidence that the extent of the G2/M arrest following irradiation is correlated with tumour cell survival and hence therapeutic success. We studied the regulation of cellular response to radiation treatment by miR-21-mediated modulation of cell cycle progression in breast cancer cells and analysed miR-21 expression in breast can...