Henrike Rebl

Rostock University Medical Faculty, Rostock, Germany · Cell Biology

Skin cancer is one of the most common malignancies worldwide. Cold atmospheric pressure Plasma (CAP) is increasingly successful in skin cancer therapy, but further research is needed to understand its selective effects on cancer cells at the molecular level. In this study, A431 (squamous cell carcinoma) and HaCaT (non-malignant) cells cultured unde...

Metals play a crucial role in the human body, especially as ions in metalloproteins. Essential metals, such as calcium, iron, and zinc are crucial for various physiological functions, but their interactions within biological networks are complex and not fully understood. Mesenchymal stem/stromal cells (MSCs) are essential for tissue regeneration du...

This protocol shows how stained and especially unstained cells can be tracked in ImageJ without much effort. The aim was to establish a reproducible setup for electrical stimulation and to perform live tracking of the stimulation with a confocal laser microscope. The study also aimed to develop a methodology for a comprehensive and standardised ass...

Background Electrical stimulation is used for enhanced bone fracture healing. Electrochemical processes occur during the electrical stimulation at the electrodes and influence cellular reactions. Our approach aimed to distinguish between electrochemical and electric field effects on osteoblast-like MG-63 cells. We applied 20 Hz biphasic pulses via...

NF-kB inhibitors (IkB) control signalling of the family of NF-kB which plays a major role in immune and stress responses. In the NCBI database, six nfkbia, twonfkbie, two nfkbid, two nfkbiz and two bcl3 genes in rainbow trout wereidentified. Salmonid fishcontain three paralogous nfkbia genes, twoof whichshareahighdegreeof sequence similarity, where...

The sensitivity to cold plasma is specific to tumor cells while leaving normal tissue cells unaffected. This is the desired challenge in cancer therapy. Therefore, the focus of this work was a comparative study concerning the plasma sensitivity of dermal tumor cells (A-431) versus non-tumorigenic dermal cells (HaCaT) regarding their adhesion capaci...

Citation: Buchwalder, S.; Hersberger, M.; Rebl, H.; Seemann, S.; Kram, W.; Hogg, A.; Tvedt, L.G.W.; Clausen, I.; Burger, J. An Evaluation of Parylene Thin Films to Prevent Encrustation for a Urinary Bladder Pressure MEMS Sensor System. Polymers 2023, 15, 3559. https://doi.org/10.3390/ polym15173559 Academic Editors: Panagiotis Barmpalexis and Konst...

Introduction: Skin cancer is often fatal, which motivates new therapy avenues. Recent advances in cancer treatment are indicative of the importance of combination treatments in oncology. Previous studies have identified small molecule-based therapies and redox-based technologies, including photodynamic therapy or medical gas plasma, as promising c...

NF-κB signalling is largely controlled by the family of ‘inhibitors of NF-κB’ (IκB). The relevant databases indicate that the genome of rainbow trout contains multiple gene copies coding for iκbα (nfkbia), iκbε (nfkbie), iκbδ (nkfbid), iκbζ (nfkbiz), and bcl3, but it lacks iκbβ (nfkbib) and iκbη (ankrd42). Strikingly, three nfkbia paralogs are appa...

In this study, a cell line of the fish species Coregonus maraena was produced for the first time. C. maraena is an endangered species, and studies indicate that this fish species will be affected by further population declines due to climate change. This cell line, designated CMAfin1, has been maintained in Leibovitz L‐15 supplemented with 10% feta...

The development of new biomaterials and medical devices has become a growing field of interdisciplinary research. The medical devices for tissue and cell treatments are being constructed for the application in regenerative medicine. There are many different approaches to improve cellular functions and it is known that physical stimuli affect cell p...

The insertion of a ureteral stent provides acute care by restoring urine flow and alleviating urinary retention or dysfunction. The problems of encrustation, bacterial colonization and biofilm formation become increasingly important when ureteral stents are left in place for a longer period of time. One way to reduce encrustation and bacterial adhe...

Due to the increasing number of human skin cancers and the limited effectiveness of therapies, research into innovative therapeutic approaches is of enormous clinical interest. In recent years, the use of cold atmospheric pressure plasma has become increasingly important as anti-cancer therapy. The combination of plasma with small molecules offers...

Four ‘protein inhibitors of activated STAT’ (PIAS) control STAT-dependent and NF-κB-dependent immune signalling in humans. The genome of rainbow trout (Oncorhynchus mykiss) contains eight pias genes, which encode at least 14 different pias transcripts that are differentially expressed in a tissue- and cell-specific manner. Pias1a2 was the most stro...

There are still numerous difficulties in the successful farming of pikeperch in the anthropogenic environment of various aquaculture systems, especially during early developmental steps in the hatchery. To investigate the physiological processes involved on the molecular level, we determined the basal expression patterns of 21 genes involved in str...

The present study provides the fundamental results for the treatment of marine organisms with cold atmospheric pressure plasma. In farmed fish, skin lesions may occur as a result of intensive fish farming. Cold atmospheric plasma offers promising medical potential in wound healing processes. Since the underlying plasma-mediated mechanisms at the ph...

Two structurally similar NF-κB-inhibitor-interacting Ras-like proteins (NKIRAS) regulate the activity of the transcription factor NF-κB and thereby control several early immune mechanisms in mammals. We identified the orthologous sequences of NKIRAS1 and NKIRAS2 from the rainbow trout Oncorhynchus mykiss. The level of sequence identity was similarl...

Encrustations of ureteral stents are one of the biggest problems with urological implants. Crystalline biofilms can occur alone or in combination with bacterial biofilms. To identify which surface parameters provide guidance for the development of novel stent materials, we used an in vitro encrustation system. Synthetic urine with increasing pH to...

Ureteric stents have become an indispensable tool in the armamentarium of every urologist. However, they carry their own morbidity resulting mostly from infectious or abacterial fouling and biofilm formation, and/or urothelial hyperplastic reaction. All of these may interact and lead to clinical complications. Many different stent designs and coati...

Recently, the potential use of cold atmospheric pressure plasma (CAP) in cancer treatment has gained increasing interest. Especially the enhanced selective killing of tumor cells compared to normal cells has prompted researchers to elucidate the molecular mechanisms for the efficacy of CAP in cancer treatment. This review summarizes the current und...

Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translate...

Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translate...

The interleukin-1-receptor-associated kinase 3 (IRAK3) is known in mammals as a negative feedback regulator of NF-κB-mediated innate-immune mechanisms. Our RNA-seq experiments revealed a prototypic 1920-nt sequence encoding irak3 from rainbow trout (Oncorhynchus mykiss), as well as 20 variants that vary in length and nucleotide composition. Based o...

Promising investigations on the selective killing of tumor cells were reported using cold atmospheric pressure plasma (CAP) in vitro. Pyruvate, a supplement in cell culture media, could protect cells against the action of reactive oxygen species. Here, we provide an overview of the in vitro studies concerning the selective killing of tumor cells by...

Biomaterials should be bioactive in stimulating the surrounding tissue to accelerate the ingrowth of permanent implants. Chemical and topographical features of the biomaterial surface affect cell physiology at the interface. A frequently asked question is whether the chemistry or the topography dominates the cell-material interaction. Recently, we...

Bacterial infection on aquaculture facilities are a major problem for productivity and quality of fish production. One of the most common and important pathogens is Aeromonas salmonicida. This Gram-negative bacterium is the causative agent of the disease furunculosis, which had a devastating impact on salmonid aquaculture up until the late 1980's....

Surface biofunctionalization is a common strategy to improve the material-tissue interface of inert implant surfaces. In this context we coated alumina-toughened zirconia (ATZ) ceramics after titanium plasma spraying with two different porous calcium phosphate layers and subsequently functionalized the obtained surfaces either with an RGD containin...

One of the most popular cell lines in osteogenesis studies is the human osteoblastic line MG‐63. For cell biological investigation, it is important that the cells remain stable in their phenotype over several passages in cell culture. MG‐63 cells can be used to provide fundamental insights into cell‐material interaction. The aim of this study is to...

Background Biomaterial modifications—chemical and topographical—are of particular importance for the integration of materials in biosystems. Cells are known to sense these biomaterial characteristics, but it has remained unclear which physiological processes bio modifications trigger. Hence, the question arises of whether the dynamic of intracellul...

The topographical and chemical surface features of biomaterials are sensed by the cells, affecting their physiology at the interface. On titanium we recently discovered an attempted caveolae-mediated phagocytosis of the sharp-edged microstructures by osteoblasts. This active, energy-consuming process resulted in decreased osteoblastic cell function...

Topographical and chemical features of biomaterial surfaces affect the cell physiology at the interface and are promising tools for the improvement of implants. The dominance of the surface topography on cell behavior is often accentuated. Striated surfaces induce an alignment of cells and their intracellular adhesion-mediated components. Recently,...

A crucial factor for ingrowth of permanent implants in the bone is the rapid cellular acceptance. The topographical features often follow mechanical aspects for implant stability. But several of these implants fail due to insufficient cell adhesion. Cells are able to perceive the physico-chemical properties of their surrounding and to pass these si...

Two ‘trout C-polysaccharide-binding proteins,’ TCBP1 and -2, with relevance to early inflammatory events have been discovered in the last century. The present study characterises the respective cDNA sequences from rainbow trout (Oncorhynchus mykiss), including multiple TCBP1 transcript variants. These variants are generated either by the use of alt...

The mammalian interleukin 1 receptor-like 1 receptor (IL1RL1), commonly known as ST2, is thought to downregulate TLR signalling by sequestering the signalling adapter MYD88 (myeloid differentiation primary response protein 88). ST2 sequences are known in several fish species, but none of them have functionally been examined. We characterised ST2 fr...

Excellent osseointegration of permanent implants is crucial for the long lasting success of the implantation. To improve the osseointegrative potential, bio-inert titanium alloy surfaces (Ti6Al4V) are modified by plasma chemical oxidation (PCO®) of the titanium-oxide layer to a non-stoichiometric, amorphous calcium phosphate layer. The native titan...

The design of a titanium implant surface should ideally support its later application in clinical use. Temporarily used implants have to fulfil requirements different from permanent implants: they should ensure the mechanical stabilization of the bone stock but in trauma surgery they should not be integrated into the bone because they will be remov...

Introduction Ureteral catheters for ureteral stenting belong to the most commonly used urological implants. They are used for temporary as well as long-term stenting of ureteral obstruction. Common complications are biofilm formation, encrustation and cellular adherence which promote urinary tract infections and cause cellular proliferation in the...

Metallic implants, especially made from titanium and its alloys, are state of the art in orthopedic and trauma surgery. Temporarily used implants such as intra-medullary nails, screws or external fixators for fractures support the mechanical stabilization of the bone consolidation but should not integrate into the bone because of their removal afte...

Bone diseases such as osteoporosis, osteoarthritis and rheumatoid arthritis, impinge on the performance of orthopaedic implants by impairing bone regeneration. For this reason, the development of effective surface modifications supporting the ingrowth of implants in morbid bone tissue is essential. Our study is designed to elucidate if cells with r...

Einleitung Harnleiterkatheter zur Schienung des Ureters gehören zu den am häufigsten eingesetzten urologischen Implantaten. Sie werden sowohl zur temporären Schienung als auch zur längerfristigen Überbrückung von Stenosen eingesetzt. Komplikationen der Ureterschienung bestehen aus Inkrustationen und zellulärer Adhärenz, welche Harnwegsinfektionen...

The long-term stability and γ-sterilisability of bioactive layers is the precondition for the application of implants. Thus, ageing processes of a microwave deposited, plasma polymerised allylamine nanofilm (PPAAm) with positively charged amino groups were evaluated concerning physico-chemical characteristics and cell adhesion capacity over the cou...

Research in plasma medicine includes a major interest in understanding gas plasma-cell interactions. The immediate application of gas plasma in vitro inhibits cell attachment, vitality and cell-cell contacts via the liquid. Interestingly, in our novel experiments described here we found that the liquid-mediated plasma effect is long-lasting after s...

Poly(L-lactide-co-D/L-lactide)-based fiber meshes resembling structural features of the native extracellular matrix have been prepared by electrospinning. Subsequent coating of the electrospun fibers with an ultrathin plasma-polymerized allylamine (PPAAm) layer after appropriate preactivation with continuous O2/Ar plasma changed the hydrophobic nat...

Titanium implant surfaces should ideally be designed to support the subsequent clinical application. Therefore temporarily used implants have to fulfill both the mechanical stabilization of the bone stock and furthermore in trauma surgery the disintegration into the bone because the implant should be removed after fracture healing. The anti-adhesiv...

Cell-biomaterial interactions are strongly affected by topographical and chemical surface characteristics. We found out earlier that geometric titanium (Ti) pillar structures in the micrometer range induce the cells to rearrange their actin cytoskeleton in short fibers solely on the top of the pillars. As a result, cell physiology was hampered conc...

Poly(L-lactide-co-D/L-lactide)-based fiber meshes resembling structural features of the native extracellular matrix have been prepared by electrospinning. Subsequent coating of the electrospun fibers with an ultrathin plasma polymerized allylamine (PPAAm) layer changed the hydrophobic nature of the polylactide surface into a hydrophilic polymer net...

Introduction: Temporary implants are of great importance in the orthopeadic field (pins, screws) as well as in the inner medicine (catheters). The common approach is to polish metal implants or to modify catheters with antibiotics to minimize protein or bacterial adhesion. However there are little approaches to clearly prevent cell adhesion by gra...

Plasma polymer deposition is the method of choice for the finishing of metallic implant materials like titanium with nitrogen-containing bioactive coatings. The deposited cell-adhesive plasma polymer films have to possess special properties such as homogeneity, film stability on air as well as in different media, sufficient density of functional gr...

Ti6Al4V foams were fabricated by the spark plasma sintering (SPS) with post-heat treatment using a blend of Ti6Al4V and sodium chloride powders. The microstructure and properties of the foams were investigated by scanning electron microscopy, X-ray micro-CT, Synchrotron X-ray, compression test and cell experiments. Results showed that the Ti6Al4V f...

Copper (Cu) could serve as antibacterial coating for Ti6Al4V implants. An additional cell-adhesive layer might compensate Cu cytotoxicity. This study aimed at in vitro and in vivo evaluation of low-temperature plasma treatment of Ti6Al4V plates with Ti/Cu magnetron sputtering (Ti6Al4V-Ti/Cu), plasma-polymerized ethylenediamine (Ti6Al4V-PPEDA), or b...

The antibacterial effect of thin titanium-copper (Ti-Cu) films combined with sufficient growth of human osteoblastic cells is reported in the paper. Thin Ti-Cu films were prepared by three different plasma-assisted magnetron sputtering methods: direct current magnetron sputtering (dc-MS), dual magnetron sputtering (dual-MS) as well as dual high pow...

Topographical and chemical modifications of biomaterial surfaces both influence tissue physiology, but unfortunately little knowledge exists as to their combined effect. There are many indications that rough surfaces positively influence osteoblast behavior. Having determined previously that a positively charged, smooth titanium surface boosts oste...

The application of antimicrobial surfaces to titanium alloy (Ti) implants would be beneficial to prevent implant-associated infections of joint endoprostheses and osteosyntheses. Copper (Cu) could be advantageously applied for this purpose, since it exhibits a well-known antimicrobial activity and is a trace element in the human body, i.e. it is no...

Differently deposited amino functionalized surfaces were compared regarding their physico-chemical surface properties in connection with cell biological response. The nitrogen containing coatings were prepared on polished titanium alloy substrates by microwave or radio frequency discharges using allylamine and ethylenediamine as precursors, respect...

Electrospinning is a versatile technique to generate tissue engineering matrices possessing structural features similar to the extracellular matrix. Biodegradable polylactides are well suited for processing by this technique, but their innate hydrophobicity impairs initial protein adsorption and cell adhesion. In this work, therefore, electrospun p...

Many functional details of the piscine Toll-like receptor (TLR) signal-mediated activation of immune defense are still elusive. We used an established reconstitution system of mammalian TLR signaling to examine if this system would allow for pathogen-dependent promoter activation of the serum amyloid A (SAA)-encoding gene from rainbow trout (Oncorh...