Henrik H J Persson

• Stanford University

Significance Quantitative detection of protein biomarkers over a wide concentration range from minute amounts of blood is essential for clinical diagnostics. Proximity ligation assay combines antibody–oligo conjugates, enzymatic ligation, and PCR amplification into a sensitive method for quantitative protein detection from small volumes. This repor...

DNA sequencing by imaging in an electron microscope is an approach that holds promise to deliver long reads with low error rates and without the need for amplification. Earlier work using transmission electron microscopes, which use high electron energies on the order of 100 keV, has shown that low contrast and radiation damage necessitates the use...

Peak-fitted high-resolution N 1s XPS spectra for single-stranded homopolymeric 20mers. The data for all samples are fit with one peak for single-bonded nitrogen at 400.6 eV. In addition the Adenine, Cytosine and Guanine samples are fit with a second peak at 399.5 eV corresponding to double-bonded nitrogen. (DOCX)

High-resolution P 2p XPS spectra for single-stranded homopolymeric 20mers. All spectra have been peakshifted to 133.6 eV and scaled to the same height. The obtained peakshift and scaling factor were used for normalization of all other elements throughout each sample. (DOCX)

Monochromatic, aberration-corrected, dual-beam low energy electron microscopy (MAD-LEEM) is a novel technique that is directed towards imaging nanostructures and surfaces with sub-nanometer resolution. The technique combines a monochromator, a mirror aberration corrector, an energy filter, and dual beam illumination in a single instrument. The mono...

of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.

Monochromatic, aberration-corrected, dual-beam low energy electron microscopy (MAD-LEEM) is a novel imaging technique aimed at high resolution imaging of macromolecules, nanoparticles, and surfaces. MAD-LEEM combines three innovative electron-optical concepts in a single tool: a monochromator, a mirror aberration corrector, and dual electron beam i...

Techniques to detect and quantify DNA and RNA molecules in biological samples have had a central role in genomics research. Over the past decade, several techniques have been developed to improve detection performance and reduce the cost of genetic analysis. In particular, significant advances in label-free methods have been reported. Yet detection...

The analysis of biomolecular interactions is key in the drug development process. Label-free biosensor methods provide information on binding, kinetics, concentration, and the affinity of an interaction. These techniques provide real-time monitoring of interactions between an immobilized ligand (such as a receptor) to an analyte in solution without...

The present study reports on the retention of conformational flexibility of a model allosteric protein upon immobilization on self-assembled monolayers (SAMs) on gold. Organothiolated SAMs of different compositions were utilized for adsorptive and covalent attachment of bovine liver glutamate dehydrogenase (GDH), a well-characterized allosteric enz...

Rapid, sequence-specific DNA detection is essential for applications in medical diagnostics and genetic screening. Electrical biosensors that use immobilized nucleic acids are especially promising in these applications because of their potential for miniaturization and automation. Current DNA detection methods based on sequencing by synthesis rely...

Magnetic MFe2O4 (M = Fe, Co, or Mn) nanoparticles with uniform diameters in the 4-20 nm range and with excellent material properties, reported previously, can be rendered soluble in water or aqueous buffers using a combination of alkylphosphonate surfactants and other surfactants such as ethoxylated fatty alcohols or phospholipids. Surfactant-modif...

With a view to the miniaturization of ion-selective electrodes (ISEs), thin (10−20 μm) polymer membranes are directly contacted to Au covered with a redox-active, lipophilic self-assembled monolayer (SAM). Several homogeneous and mixed monolayers are characterized by reflection−absorption infrared spectroscopy, ellipsometry, scanning tunneling micr...

Self-assembled monolayers (SAMs) of 11-mercapto-1-undecanol (TOH) and mixed monolayers of TOH and dodecanethiol (TCH3) were transformed to isocyanate-bearing layers by reaction of the hydroxyl groups in the monolayers and mixed layers with 1,4-phenylene diisocyanate (PDI). PDI reacts with only one of its isocyanate groups, yielding a carbamate (ure...

This study investigates the importance of implant surface topography on soft tissue response. The tissue response in the rat abdominal wall to discs of low density polyethylene with smooth to coarse surfaces was evaluated after one, six or 12 weeks. Capsule thickness and immunohistochemical quantification of monocytes-macrophages were used as measu...