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Publication (1)
Providing the right stimulatory conditions resulting in efficient tissue promoting microenvironment in vitro and in vivo is one of the ultimate goals in tissue development for regenerative medicine. It has been shown that in addition to molecular signals (e.g. growth factors) physical cues are also required for generation of functional cell constru...
Questions
Questions (5)
Hello,
I'm building a new patch-clamp rig and wanted to know if anyone can compare the two of these.
I'm interested in the user interface, stability, and overall experience.
Many thanks
Hemi
I'm using heavily doped p-type silicon (boron), and i was wondering if there is a dopant redistribution at the surface of the etched substrate.
I was able to find this for wet thermal growth of SiO2, but not for etching silicon.
Any ideas will be much appreciated.
Thanks
Hemi
Hi all,
I'm using a vibratome to cut heart slices, and I want to do calcium imaging of the slices. I know for sure that the tissue is alive, as it is obviously contacting upon stimulation (without BDM).
However, I can't get any calcium waves from it. I stained the slices with fluo -4 or Rhode 2 (5 uM, with pluronic), and also tried to load the dye using layendorff perfused heart and then cut it to slices.
Love imaging didn't give any flurescence changes.
Will be grateful for any suggestions.
Thanks a lot
Hemi
I'm using an isolated heart model by perfusing the heart with oxygenated Hepes buffered Tyrode's Solution (the heart is contracting and looks healthy), and then I use vibratome to cut 350-500 um slices of rat heart (cutting in ice cold HBSS).
Then i transfer them to a warm oxygenated Hepes buffered Tyrode's Solution.
Should the slices contract spontaneously? or do i need to provide electrical stimulation?
I'm trying to see expression of Cn-43 in rat cardiomyocytes, using flow cytometry.
i was also hoping to see expression in the cardiac fibroblasts that are also in the co-culture.
Did anyone try to do this? what was the antibody, and the staining protocol?
I tried it, but couldn't see nothing. staining or cardiac troponin was week, and for vimentin (fibroblasts) pretty strong.
I used trypsin (is that OK?) to harvest the cells, and a conjugated antibody for human Cn-43 (should have 98% homology to rat's).
Thanks a lot
Hemi
