Helle Stendahl Andersen

Helle Stendahl Andersen
Danish Technological Institute · Laboratory for Chemistry and Microbiology

M. Sc

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An oxygen-limited fed-batch technique (OLFB) was compared to traditional methanol-limited fed-batch technique (MLFB) for the production of recombinant Thai Rosewood β-glucosidase with Pichia pastoris. The degree of energy limitation, expressed as the relative rate of respiration (q O/q O,max), was kept similar in both the types of processes. Due to...

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Question
I would like to hear if there is anyone who has experience with EN 13697 (standard for surface disinfection). I have noticed that if we make a suspension test for a disinfectant (e.g. EN 1276) where it is no problem to demonstrate a log 5 reduction: When we use the same product for EN 13697 the bacteria all the sudden survive. Does anyone have an explanation for that? Especially P.aeruginosa is a problem - sometimes it seems like it is capable of using the disinfectant as a nutrient. Is that possible?

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