Hélder A Santos

Hélder A Santos
University of Groningen | RUG · Department of Biomedical Engineering

D.Sc. (Chem. Eng.)

About

471
Publications
86,387
Reads
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16,072
Citations
Citations since 2016
333 Research Items
13995 Citations
201620172018201920202021202205001,0001,5002,0002,5003,000
201620172018201920202021202205001,0001,5002,0002,5003,000
201620172018201920202021202205001,0001,5002,0002,5003,000
201620172018201920202021202205001,0001,5002,0002,5003,000
Introduction
Dr. Hélder A. Santos (http://www.helsinki.fi/~hsantos) is a physical chemist specialized in electrochemistry and Pharmaceutical Nanotechnology. In 2007, he obtained his DSc in Chem. Eng. from the Helsinki University of Technology. Currently, he is a Full Professor in Pharmaceutical Nanotechnology, Head of the Nanomedicine and Biomedical Engineering Lab, and Director of the Doctoral Programme in Drug Research, at the Faculty of Pharmacy, Univ. Helsinki.
Additional affiliations
April 2009 - December 2020
University of Helsinki
Position
  • Professor (Full)

Publications

Publications (471)
Article
Full-text available
The use of amphiphilic block copolymers to generate colloidal delivery systems for hydrophobic drugs has been the subject of extensive research, with several formulations reaching the clinical development stages. However, to generate particles of uniform size and morphology, with high encapsulation efficiency, yield and batch-to-batch reproducibili...
Article
Full-text available
Tumor Therapy A pH‐responsive cluster metal–organic framework nanoparticle is developed by Wenguo Cui, Baiyong Shen, Hélder A. Santos, and co‐workers in article number 2203915, based on acid‐induced conversion from hydrophilicity to hydrophobicity, leading to aggregated nanoparticles. These nanoparticles inhibit tumor growth and reactivate the tumo...
Article
Full-text available
Redox‐responsive silica drug delivery systems are synthesized by aeco‐friendly diatomite source to achieve on‐demand release of peptide nucleic acid (PNA) in tumor reducing microenvironment, aiming to inhibit the immune checkpoint programmed cell death 1 receptor/programmed cell death receptor ligand 1 (PD‐1/PD‐L1) in cancer cells. The nanoparticle...
Article
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Porous silicon (PSi) nanoparticles have been applied in various fields, such as catalysis, imaging, and biomedical applications, because of their large specific surface area, easily modifiable surface chemistry, biocompatibility, and biodegradability. For biomedical applications, it is important to precisely control the surface modification of PSi-...
Article
Full-text available
As a result of the deficient tumor‐specific antigens, potential off‐target effect, and influence of protein corona, the metal‐organic framework nanoparticles have inadequate accumulation in the tumor tissues, limiting the therapeutic effects. In this work, a pH‐responsive linker (L) is prepared by covalently modifying oleylamine (OA) with 3‐(bromom...
Cover Page
Full-text available
Cardiac Repair In article number 2204666, Qiang Zhao, Xiaofeng Ye, Xianming Deng, Hélder A. Santos, and co-workers introduce a microfluidics-assisted single step, green synthesis method for producing targeting ligands free heart homing nanoparticles in a tailored manner. The developed microfluidic platform facilitates an economically feasible and...
Article
Cardiac Repair In article number 2204666, Qiang Zhao, Xiaofeng Ye, Xianming Deng, Hélder A. Santos, and co‐workers introduce a microfluidics‐assisted single step, green synthesis method for producing targeting ligands free heart homing nanoparticles in a tailored manner. The developed microfluidic platform facilitates an economically feasible and s...
Article
Full-text available
Many drugs with anticancer potential fail in their translation to the clinics due to problems related to pharmacokinetics. LEM2 is a new dual inhibitor of MDM2/mutp53-TAp73 interactions with interesting in vitro anticancer activity, which opens new hopes as an unconventional anticancer therapeutic strategy against cancers lacking p53 or with impair...
Article
Micrometer‐sized hydrogels are crosslinked three‐dimensional network matrices with high‐water contents and dimensions ranging from several to hundreds of micrometers. Due to their excellent biocompatibility and capability to mimic physiological microenvironments in vivo, micrometer‐sized hydrogels have attracted much attention in the biomedical eng...
Article
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Macrophage targeting and researches centered on immunological responses have received interest thanks to studies unveiling the significant role of macrophages in inflammatory diseases and cancer. In this regard, we have selected four types of nanoparticles (NPs), including acetalated dextran-based nano-carrier functionalized with atrial natriuretic...
Article
In this work, we describe the synthesis of magnetic nanoparticles composed of a maghemite core (MNP) and three different coatings (dextran, D-MNP; carboxymethyldextran, CMD-MNP; and dimercaptosuccinic acid, DMSA-MNP). Their interactions with red blood cells, plasma proteins, and macrophages were also assessed. CMD-MNP was selected for its good bios...
Article
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Nanoparticle (NP)‐based intravenous administration represents the most convenient cardiac targeting delivery routine, yet, there are still therapeutic issues due to the lack of targeting efficiency and specificity. Active targeting methods using functionalization of ligands onto the NPs’ surface may be limited by trivial modification procedures and...
Article
Full-text available
In late 2019, coronavirus disease 2019 (COVID-19) was caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Spike protein is one of the surface proteins of SARS-CoV-2 that is essential for its infectious function. Therefore, it received lots of attention for the preparation of antiviral drugs, vaccines, and diagnostic tools. In th...
Article
Full-text available
Artificial intelligence (AI) methods are explosively considered in the design and optimization of drug discovery and delivery systems. Herein, machine learning methods are used for optimizing the production of curcumin (CUR)-loaded nanofibers. The required data are mined through the literature survey and two categories, including material- and mach...
Article
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Fibrotic diseases remain a substantial health burden with few therapeutic approaches. A hallmark of fibrosis is the aberrant activation and accumulation of myofibroblasts, which is caused by excessive profibrotic cytokines. Conventional anticytokine therapies fail to undergo clinical trials, as simply blocking a single or several antifibrotic cytok...
Article
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Biopolymeric patches show enormous potential for the regeneration of infarcted myocardium tissues. However, most of them usually lack appropriate mechanical performance, stability in water, and important functionalities; for instance, antioxidant activity. Protein nanofibrils, such as lysozyme nanofibrils (LNFs), are biocompatible nanostructures wi...
Article
Full-text available
Traditional bolus vaccines often fail to sustain robust adaptive immune responses, typically requiring multiple booster shots for optimal efficacy. Additionally, these provide few opportunities to control the resulting subclasses of antibodies produced, which can mediate effector functions relevant to distinct disease settings. Here, it is found th...
Article
A surface adsorption strategy is developed to enable the engineering of microcomposites featured with ultrahigh loading capacity and precise ratiometric control of co-encapsulated peptides. In this strategy, peptide molecules (insulin, exenatide, and bivalirudin) are formulated into nanoparticles and their surface is decorated with carrier polymers...
Article
The cytokine storm caused by SARS-CoV-2 infection threatens the condition of patients, even leading to death. In a recent issue of Matter, Prof. Wenguo Cui and co-workers have prepared lung-sweeper inhaled hydrogel microspheres for intratracheal neutralization of COVID-19 and cytokine storm calming, which could be applied for antiviral tissue regen...
Article
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Cardiovascular and cerebrovascular diseases (CCVDs) describe abnormal vascular system conditions affecting the brain and heart. Among these, ischemic heart disease and ischemic stroke are the leading causes of death worldwide, resulting in 16% and 11% of deaths globally. Although several therapeutic approaches are presented over the years, the cont...
Article
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Drug delivery systems with high content of drug can minimize excipients administration, reduce side effects, improve therapeutic efficacy and/or promote patient compliance. However, engineering such systems is extremely challenging, as their loading capacity is inherently limited by the compatibility between drug molecules and carrier materials. To...
Article
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Thrombosis and infections are the two major complications associated with extracorporeal circuits and indwelling medical devices, leading to significant mortality in clinic. To address this issue, here, we report a biomimetic surface engineering strategy by the integration of mussel-inspired adhesive peptide, with bio-orthogonal click chemistry, to...
Article
Full-text available
Here, an alternative strategy of choosing photothermal and weak-immunostimulatory porous silicon@Au nanocomposites as particulate cores to prepare a biomimetic nanovaccine, is reported to improve its biosafety and immunotherapeutic efficacy for solid tumors. A quantitative analysis method is used to calculate the loading amount of cancer cell membr...
Cover Page
Full-text available
A cancer cell membrane@porous silicon nanoparticles@Au nanovaccine is developed by Zhenyang Luo, Hélder A. Santos, Bing Xia, and co-workers in article number 2108012, based on weak-immunostimulatory porous silicon@Au nanoparticulate cores and multi-antigenic cancer cell membranes. These exogenous nanovaccines boost antitumor immunity, preventing th...
Article
Full-text available
Oral insulin delivery could change the life of millions of diabetic patients as an effective, safe, easy-to-use, and affordable alternative to insulin injections, known by an inherently thwarted patient compliance. Here, we designed a multistage nanoparticle (NP) system capable of circumventing the biological barriers that lead to poor drug absorpt...
Article
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The development of cell membrane-modified biomimetic nanoparticles has extensively increased during the past years due to their exceptional biocompatibility, evasion form immune system, and targeting ability. Known as a cutting-edge area of research in nanomedicine, such novel nanoplatforms can mimic different functions of the primary cells, while...
Article
Nanovaccines A cancer cell membrane@porous silicon nanoparticles@Au nanovaccine is developed by Zhenyang Luo, Hélder A. Santos, Bing Xia, and co‐workers in article number 2108012, based on weak‐immunostimulatory porous silicon@Au nanoparticulate cores and multi‐antigenic cancer cell membranes. These exogenous nanovaccines boost antitumor immunity,...
Article
Full-text available
Myocardial infarction results in a massive loss of cardiomyocytes (CMs). Unfortunately, current therapies are unsuccessful in replacing lost CMs, and thus, there is an urgent need for innovative approaches. Here, a nanosystem based on spermine-acetalated dextran (AcDXSp) and encapsulating two drug compounds able to stimulate in vitro CMs proliferat...
Article
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Diabetes mellitus (DM) refers to a group of metabolic disorders that are characterized by hyperglycemia. Oral subcutaneously administered antidiabetic drugs such as insulin, glipalamide, and metformin can temporarily balance blood sugar levels, however, long-term administration of these therapies is associated with undesirable side effects on the k...
Article
Neutrophils are the most abundant innate immune cells in human circulation; however, their derived exosomes have been rarely studied for tumor treatment. Here, we reported that exosomes from neutrophils (N-Ex) induce tumor cell apoptosis by delivering cytotoxic proteins and activating caspase signaling pathway. In addition, we decorated N-Ex with s...
Article
Full-text available
The natural healing capacity of the tendon tissue is limited due to the hypovascular and cellular nature of this tissue. So far, several conventional approaches have been tested for tendon repair to accelerate the healing process, but all these approaches have their own advantages and limitations. Regenerative medicine and tissue engineering are in...
Article
Solid lipid nanoparticles (SLNs) can combine the advantages of different colloidal carriers and prevent some of their disadvantages. The production of nanoparticles by means of microfluidics represents a successful platform for industrial scale-up of nanoparticle manufacture in a reproducible way. The realisation of a microfluidic technique to obta...
Book
Porous Silicon for Biomedical Applications, Second Edition, provides an updated guide to the diverse range of biomedical applications of porous silicon, from biosensing and imaging to tissue engineering and cancer therapy. Across biomedical disciplines, there is an ongoing search for biomaterials that are biocompatible, modifiable, structurally so...
Article
Full-text available
Polyoxometalates are an emerging class of molecular clusters, with well-defined structures and chemical compositions that are produced through simple, low-cost, and highly reproducible methods. In particular, the wheel-shaped cluster {Mo154} is a promising photothermal agent due to its intervalence charge transfer transitions. However, its toxicity...
Cover Page
Full-text available
Photothermal Materials In article number 2007761, Shiqi Wang, Hélder Santos, and co-workers introduce a polyoxometalate {Mo154}-incorporating, dual-crosslinked dynamic hydrogel (Mo154Gel) formed by Schiff base and electrostatic interactions. Mo154Gel shows exceptional photothermal conversion, and pH- and near-infrared-laser-triggered drug release....
Article
Nanomedicines represent innovative and promising alternative technologies to improve the therapeutic effects of different drugs for cancer ablation. Targeting M2-like tumor-associated macrophages (TAMs) has emerged as a favorable therapeutic approach to fight against cancer through the modulation of the tumor microenvironment. However, the immunomo...
Article
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Immune cells play a crucial regulatory role in inflammatory phase and proliferative phase during skin healing. How to programmatically activate sequential immune responses is the key for scarless skin regeneration. In this study, an “Inner-Outer” IL-10-loaded electrospun fiber with cascade release behavior was constructed. During the inflammatory p...
Article
Nanostructures based on DNA self-assembly present an innovative way to address the increasing needs for target-specific delivery of therapeutic molecules. Currently, most of the chemotherapeutics being used in clinical practice have undesired and exceedingly high off-target toxicity. This is a challenge in particular for small molecules, and hence,...
Technical Report
Full-text available
Research on microneedle patches for vaccine delivery has grown in popularity in recent years, due to their exceptional compliance and low invasiveness.
Chapter
During the last decades, different nanosystems have been developed to deliver therapeutic agents and improve their solubility and pharmacokinetic properties, reducing their systemic side effects. To achieve this, nanomedicines need to be rationally designed and tailored to reach a specific site of action, modulate their biodistribution, and circumv...
Chapter
In recent years, the emergence of the energy crisis, pollution, waste management, and the shortage of raw materials has become a major concern globally. Lignocellulosic materials have been examined as naturally alternative resources obtained from bioenergy crops and agricultural residues attempting for the replacement of petroleum-derived products...
Article
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Although great promise has been achieved with nanomedicines in cancer therapy, limitations are still encountered, such as short retention time in the tumor. Herein, a nanosystem that can modulate the particle size in situ by near‐infrared (NIR) light is self‐assembled by cross‐linking the surface‐modified poly(lactic‐co‐glycolic acid) from the up‐c...
Cover Page
Full-text available
In article number 2101262, Gang Chen, Hélder A. Santos, Wenguo Cui, and co-workers construct a light-controlled nanosystem with in situ modulated particle size by near-infrared irradiation with a synergistic effect using photochemotherapy for tumor suppression. The nanosystem at the tumor leads to rapid shedding of polye thylene glycol by near-infr...
Preprint
Full-text available
In late 2019, coronavirus disease 2019 (COVID-19) caused severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Spike protein is one of the surface proteins of SARS-CoV-2 that is essential for its infectious function. Therefore, it received lots of attention for the preparation of antiviral drugs, vaccines, and diagnostic tools. Herein, we u...
Article
Full-text available
Selenium (Se) is an essential element to human health that can be obtained in nature through several sources. In the human body, it is incorporated into selenocysteine, an amino acid used to synthesize several selenoproteins, which have an active center usually dependent on the presence of Se. Although Se shows several beneficial properties in huma...
Article
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Communication between biological components is critical for homeostasis maintenance among the convergence of complicated bio-signals. For therapeutic nanoparticles (NPs), the general lack of effective communication mechanisms with the external cellular environment causes loss of homeostasis, resulting in deprived autonomy, severe macrophage-mediate...
Article
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Ribonucleic acid interference (RNAi) is an innovative treatment strategy for a myriad of indications. Non-viral synthetic nanoparticles (NPs) have drawn extensive attention as vectors for RNAi due to their potential advantages, including improved safety, high delivery efficiency and economic feasibility. However, the complex natural process of RNAi...
Article
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Metastatic melanoma can be difficult to detect until at the advanced state that decreases the survival rate of patients. Several FDA-approved BRAF inhibitors have been used for treatment of metastatic melanoma, but overall therapeutic efficacy has been limited. Lutetium-177 (177Lu) enables simultaneous tracking of tracer accumulation with single-ph...
Article
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Gene therapy is identified as a powerful strategy to overcome the limitations of traditional therapeutics to achieve satisfactory effects. However, various challenges related to the dosage form, delivery method, and, especially, application value, hampered the clinical transition of gene therapy. Here, aiming to regulate the cartilage inflammation...
Article
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Correction for 'Acetalated dextran based nano- and microparticles: synthesis, fabrication, and therapeutic applications' by Shiqi Wang et al., Chem. Commun., 2021, DOI: 10.1039/d1cc00811k.
Article
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Electrospun organic/inorganic hybrid scaffolds have been appealing in tissue regeneration owing to the integrated physiochemical and biological performances. However, the conventional electrospun scaffolds with non-woven structures usually failed to enable deep cell infiltration due to the densely stacked layers among the fibers. Herein, through se...
Cover Page
Full-text available
Acetalated dextran (Ac-DEX) is a pH-responsive dextran derivative polymer. Ac-DEX and its functional derivatives have been formulated as nano- or microparticles for drug delivery applications in the biomedical field.