Heidi Goenaga Infante

Heidi Goenaga Infante
LGC · Mass Spectrometry - Chemical Measurement & Calibration

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148
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Publications

Publications (148)
Article
Molybdenum is an essential dietary trace element required for several critical enzyme systems. High intake is associated with toxicity in ruminants and animal studies. The proposed therapeutic use of molybdenum based drugs poses a potential risk for accumulation through chronic administration of therapeutic doses of this element. The current experi...
Article
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Porous hollow silica particles possess promising applications in many fields, ranging from drug delivery to catalysis. From the synthesis perspective, the most challenging parameters are the monodispersity of the size distribution and the thickness and porosity of the shell of the particles. This paper demonstrates a facile two-pot approach to prep...
Article
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We describe the outcome of a large international interlaboratory study of the measurement of particle number concentration of colloidal nanoparticles, project 10 of the technical working area 34, "Nanoparticle Populations" of the Versailles Project on Advanced Materials and Standards (VAMAS). A total of 50 laboratories delivered results for the num...
Article
Main text The CCQM Isotope Ratio Working Group (IRWG) determined that an additional key comparison of carbon isotope delta measurements was required to capture the progress of this field. Vials containing 0.25 mg of vanillin were prepared at NRC, and evaluated for bottle-to-bottle homogeneity prior to distribution to the eight participating institu...
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Background Safe and rational development of nanomaterials for clinical translation requires the assessment of potential biocompatibility. Autophagy, a critical homeostatic pathway intrinsically linked to cellular health and inflammation, has been shown to be affected by nanomaterials. It is, therefore, important to be able to assess possible intera...
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This paper describes Food-MetNet, a coordinated preparatory initiative to establish the European Metrology Network on Food Safety (EMN-FS). Food-MetNet aims to establish a long-term ongoing dialogue between the metrology community and relevant stakeholders, in particular, European Union Reference Laboratories (EURLs), National Reference Laboratorie...
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Rationale: Preparation of in-house reference materials (RMs) is an important aspect of light element stable isotope analysis. While some relevant information is available, there is, as yet no clear set of guidelines available covering all aspects of in-house RM production and characterization. Methods: To address this need, the experience of pro...
Article
Species-specific isotope dilution mass spectrometry (SS-IDMS) has been the calibration method of choice for high accuracy speciation analysis because it can correct for detector sensitivity drifts, matrix effects, and analyte loss during sample preparation and analysis. However, in many cases SS-IDMS calibration is either not applicable (e.g. for m...
Article
Exchangeable copper (CuEXC), mainly comprised copper (Cu) bound to albumin, has been proposed as a specific marker of Cu overload in Wilson’s disease (WD). To the author’s knowledge, there are no methods capable of determining reliably CuEXC to meet the requirements and challenges faced by a clinical trial. The present work describes a novel specia...
Article
Recommendations are given concerning the terminology of concepts and methods used in spectroscopy in analytical chemistry, covering nuclear magnetic resonance spectroscopy, atomic spectroscopy, and vibrational spectroscopy.
Article
A method based on asymmetric flow field-flow fractionation (AF4) coupled to ultraviolet-visible (UV-vis) spectroscopy and inductively coupled plasma mass spectrometry (ICP-MS) has been developed for silver nanoparticles (Ag NPs) detection and quantification in bivalve molluscs. Samples were pre-treated using a conventional enzymatic (pancreatin and...
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Toxicity of methylmercury (MeHg) to wildlife and humans results from its binding to cysteine residues of proteins, forming MeHg-cysteinate (MeHgCys) complexes that hinder biological functions. MeHgCys complexes can be detoxified in vivo, yet how this occurs is unknown. We report that MeHgCys complexes are transformed into selenocysteinate [Hg(Sec)4...
Article
There is evidence that iron (Fe) and selenium (Se) are dysregulated in neurodegenerative diseases (ND). Disease-specific impacts on concentration distributions are anticipated for Fe (the most abundant transition metal), and...
Article
Toxicological studies concerning nanomaterials in complex biological matrices usually require a carefully designed workflow that involves handling, transportation and preparation of a large number of samples without affecting the nanoparticle...
Chapter
Field-flow fractionation (FFF) in combination with elemental and sizing detectors has emerged as a tool of great potential for the characterisation of inorganic nanomaterials in complex samples, mainly in the fields of environment, consumer products and bioanalysis, over the past 10 years. Given the complexity of the combination analyte/matrix, the...
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We demonstrate a novel way to introduce a so-called Hg reference gas, produced by a gas generator, into an elemental detector, such as ICP-MS, and to directly measure gaseous oxidized mercury (GOM as HgCl2) at a wide range of concentrations, covering 4 orders of magnitude. The approach was tested using two types of generators based on the dilution...
Article
Online normalisation to isotope ratio of admixed internal standards is an important technique in isotopic analysis by multicollector ICP-MS (MC-ICP-MS). It is widely used for minimising drift in measurements of δ-values and calibrating isotope amount ratios for medium and heavy mass elements. However, its potential has been underexplored for light...
Article
This work represents the first systematic speciation study of selenium (Se) in plasma from subjects participating in a pilot study for a cancer prevention trial (PRECISE). This involved supplementation of elderly British and Danish individuals with selenised yeast for 6 months and 5 years, respectively, at 100, 200, and 300 μg Se/day or placebo. Sp...
Article
This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal
Article
A Study on the Analysis of Particle Size Distribution for Bimodal Model Nanoparticles by Electron Microscopy - Christoph Hörenz, Olivier Tache, Dorota Bartczack, Susana Nunez, Isabel Abad Alvaro, Heidi Goenaga-Infante, Vasile-Dan Hodoroaba
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Methodology for the accurate determination of number concentration of inorganic nanoparticles (NP) by single particle inductively coupled plasma mass specrometry (spICP-MS) using the novel dynamic mass flow (DMF) approach is systematically described. Using this method the determination of transport efficiency (TE) is achieved without the need for a...
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Determination of the purity of a substance traceable to the International System of Units (SI) is important for the production of reference materials affording traceability in quantitative measurements. Post-column isotope dilution using liquid chromatography-chemical oxidation-isotope ratio mass spectrometry (ID-LC-CO-IRMS) has previously been sug...
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Rationale Analytical methods that can identify the source and fate of mercury and organomecury compounds are likely to be useful tools to investigate mercury in the environment. Carbon isotope ratio analysis of methylmercury (MeHg) together with mercury isotope ratios may offer a robust tool to study environmental cycling of organomercury compounds...
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The industrial exploitation of high value nanoparticles is in need of robust measurement methods to increase the control over product manufacturing and to implement quality assurance. InNanoPart, a European metrology project responded to these needs by developing methods for the measurement of particle size, concentration, agglomeration, surface ch...
Article
Measuring the number concentration of colloidal nanoparticles is critical for assessing reproducibility, compliance with regulation and performing risk assessments of nanoparticle-enabled products. For nanomedicines, their number concentration directly relates to their dose. However, the lack of relevant reference materials and established traceabl...
Article
This work demonstrates for the first time the potential of monolithic chromatography coupled to double species-specific IDA ICPMS for the detection, and accurate quantification of adducts of plasma/serum proteins with...
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Mesenchymal Stromal Cells (MSCs) have become a promising candidate for autologous cell therapy for a number of human pathologies, most notably in the treatment of auto-immune diseases. However, to achieve effective clinical dosing, relatively large numbers of these cells are required, making extensive in vitro expansion paramount. This poses an inc...
Article
Lead isotope amount ratios are commonly used in diverse fields such as archaeometry, geochemistry and forensic science. Currently, five reference materials with certified lead isotope amount ratios are available, namely NIST SRM 981, 982 and 983, GBW‐04442 and NMIJ 3681‐a. Only NIST SRM 981 and NMIJ 3681‐a have approximately natural isotopic compos...
Article
Exposure to titanium (Ti), via the ingestion of pigment grade Ti dioxide (TiO2), is commonplace for Westernised populations. It may also occur as a consequence of metal ion leaching in...
Article
Species-specific Hg isotope ratio data has increasingly become an important tool in understanding biogeochemistry of mercury.
Article
Fractionation data for cadmium in tobacco products, as obtained by sequential leaching of cadmium species with ICP-MS/MS analysis, and separately by X-ray absorption near edge structure (XANES) are presented here for the first time. The total amount of cadmium found in 3R4F cigarette cut tobacco was 1526 ± 42 μg kg−1, of which 5% was found in the s...
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Forensic application of carbon isotope ratio measurements of honey and honey protein to investigate the degree of adulteration with high fructose corn syrup or other C4 plant sugars is well established. These measurements must use methods that exhibit suitable performance criteria, particularly with regard to measurement uncertainty and traceabilit...
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Background: Genetic testing of tumor tissue and circulating cell-free DNA for somatic variants guides patient treatment of many cancers. Such measurements will be fundamental in the future support of precision medicine. However, there are currently no primary reference measurement procedures available for nucleic acid quantification that would sup...
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The factors that affect the accuracy and precision of differential centrifugal sedimentation (DCS) for the analysis of nanoparticle concentration are described. Particles are separated by their sedimentation rate and detected using light absorption. In principle, the relative concentration of particles in different populations can be found, but the...
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Monitoring physicochemical characteristics of nanoparticles following internalisation by cells is a vital step in understanding their biological impact and toxicity. Here, the feasibility of methodology utilising gentle enzymatic lysis of cells containing internalised particles and direct analysis of the lysates for particles size, agglomeration st...
Article
For the first time, an international comparison was conducted on the determination of the purity of a high purity element. Participants were free to choose any analytical approach appropriate for their institute's applications and services. The material tested was a high purity zinc, which had earlier been assessed for homogeneity and previously us...
Article
Elements in human serum serve as important biomarkers and their levels reflect the well-being of an individual. Electrolytes such as sodium (Na) and chloride (Cl) are crucial in maintaining the normal distribution of water, osmotic pressure and electrical neutrality in the body. Trace element such as copper (Cu) plays a part in many oxidation-reduc...
Article
Variations in the stable isotopic composition of carbon are commonly expressed as δ(¹³C) values, and are used in diverse fields, including biology, environmental sciences, food and drug authentication and forensic applications. Progress in these fields requires accurate and comparable stable carbon isotope ratio measurements between laboratories an...
Presentation
Regulation concerning the commercial use of nanomaterials (NM) state that their presence should now be clearly indicated on the label of food and cosmetic products marketed in the European Union (EU). EU Regulation is based on the definition of NM1, describing it as a material with 50% (1-50% in specific cases) or more particles with one or more ex...
Article
Long-lived high abundance radionuclides are of increasing interest with regard to decommissioning of nuclear sites and longer term nuclear waste storage and disposal. In many cases, no routine technique is available for their measurement in nuclear waste and low-level (ng kg⁻¹) environmental samples. Recent advances in ICP-MS technology offer attra...
Article
Iodine deficiency is present in certain groups of the UK population, notably in pregnant women; this is of concern as iodine is required for fetal brain development. UK milk is rich in iodine and is the principal dietary iodine source. UK sales of milk-alternative drinks are increasing but data are lacking on their iodine content. As consumers may...
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Platinum-DNA adducts, and in particular Pt-GG, has been identified as the major cytotoxic species during chemotherapy treatment with platinum containing drugs. This paper reports for the first time a strategy based on the use of double species-specific isotope dilution analysis (IDA) for the quantification of carboplatin-GG adducts formed by exposi...
Article
As there can be small but measureable differences in isotope ratios between different sources of the same element/compound/material, isotope ratio measurements are applied in a number of different fields including archaeology, environmental science, geochemistry, forensic science and ecology. Isotope ratios for the light elements (H, C, N, O and S)...
Article
CCQM-K124 was organized by the Inorganic Analysis Working Group (IAWG) of CCQM to assess and document the capabilities of the national metrology institutes (NMIs) or the designated institutes (DIs) to measure the mass fractions of trace elements (As, B, Cd, Ca, Cr, Hg and Mo) and hexavalent chromium (Cr(VI)) in drinking water. The National Metrolog...
Article
CCQM-K125 was organized by the Inorganic Analysis Working Group (IAWG) of CCQM to assess and document the capabilities of the national metrology institutes (NMIs) or the designated institutes (DIs) to measure the mass fractions of trace elements (K, Cu and I) in infant formula. Government Laboratory, Hong Kong SAR (GLHK) acted as the coordinating l...
Article
A novel strategy is reported for the quantitative analysis of the Fe spatial distribution in biological tissue using laser ablation with ICP-MS and on-line double isotope dilution analysis (LA-ICP-IDMS). The proposed on-line IDMS method involves post-ablation introduction of an isotopically enriched 57Fe spike solution using a total consumption neb...
Article
Improved methodology for high accuracy Mo isotope amount ratio measurements by multi-collector inductively coupled plasma mass spectrometry (MC-ICPMS) using normalisation to an internal standard is presented. It is based on the use of the regression model to correct for instrumental mass discrimination with NIST Standard Reference Material 987, cer...
Chapter
This chapter discusses the advances and the challenges of quality control in speciation measurements with focus on the role of isotope dilution quantification in the provision of reference values for the characterization of reference materials and for validation of more routinely speciation measurements. It explains the synergetic use of elemental...
Article
In this work, we present the first methodical approach to the preparation, molecular characterization and use of a novel type of full-length selenoprotein standard as exemplified for the human plasma selenoproteins, glutathione peroxidase 3 (GPx3) and selenoprotein P (SEPP1), both in non-labeled and stable isotope-labeled forms. Their production wa...
Article
A species-specific isotope dilution analysis (SS IDA) method was developed for the first time for the determination of selenoprotein P (SEPP1) in human plasma/serum at the protein level by double affinity HPLC-ICP-MS. In this regard, a standard and a spike of SEPP1 were produced by cell-free E. coli protein synthesis, where Se (ICP tag) was introdu...
Article
Guest Editors, Heidi Goenaga-Infante and Gunda Koellensperger, introduce the 2016 speciation themed issue.
Article
Plain and aminated silica nanoparticles dispersed in purified water, in 50 mM Tris-HCl buffer and in cell culture medium were measured using dynamic light scattering (DLS), centrifugal liquid sedimentation (CLS), small-angle X-ray scattering (SAXS), and particle tracking analysis (PTA). The test samples were measured by all methods immediately afte...
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A novel strategy for the absolute quantification of selenium (Se) included in Selenoprotein P (SEPP1), an important bio-marker for human nutrition and disease, including diabetes and cancer, is presented here for the first time. It is based on the use of species-specific double isotope dilution mass spectrometry (SSIDA) in combination with HPLC-ICP...
Chapter
This chapter provides an overview of the state of the art of currently available techniques capable of performing number-based measurements on nanomaterial suspensions. Single-particle inductively coupled plasma mass spectrometry (spICP-MS) is a recently emerged method, which leverages on traditional ICP-MS measurement principle. One of the key adv...
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Polybrominated diphenylethers (PBDE), used as flame retardants, are named as priority substances in the Directive 2000/60/EC of the European parliament and of the council establishing a framework for Community action in the field of water policy. An annual average environmental quality standard (EQS) for inland surface waters of 0.0005µg/L (0.0002µ...
Article
A total of eleventh NMIs participated in CCQM-K107. The sample is frozen human serum prepared from blood obtained from the Royal Surrey County Hospital. It HSA been stored in 2 mL plastic screw-cap Nalgene cryovial, each containing 1.1 mL serum, at -70�C � 10�C in the dark. The serum sample HSA been tested for homogeneity with 0.5g subsamples...
Article
div class="title">Iodine concentration of milk-alternative drinks available in the UK - Volume 75 Issue OCE3 - S.C. Bath, S. Hill, H. Goenaga-Infante, S. Elghul, M.P. Rayman
Article
Speciation data for chromium in tobacco products, as obtained by complementary HPLC-ICP-MS and synchrotron-based X-ray Absorption Near-Edge Structure spectroscopy (XANES), are presented for the first time. Non-denaturing extraction conditions were investigated to avoid Cr species redox inter-conversion before analysis of extracts using HPLC-ICP-MS....
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This paper describes the production and characteristics of the nanoparticle test materials prepared for common use in the collaborative research project NanoChOp (Chemical and optical characterization of nanomaterials in biological systems), in casu suspensions of silica nanoparticles and CdSe/CdS/ZnS quantum dots (QDs). This paper is the first to...
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The surface charge density of nanoparticles plays an important role in the way they interact with biological systems. The ability to measure the surface charge density of nanoparticles in biological media is therefore of importance in understanding the magnitude of such interactions. There are a number of methods which may be used to assess surface...
Article
We propose for the first time methodology for the determination of number-based concentration of silica (SiO2) nanoparticles (NP) in biological serum using nanoparticle tracking analysis (NTA) as on-line detector for asymmetric flow field-flow fractionation (AF4). The degree of selectivity offered by AF4 was found necessary to determine reliably nu...
Article
A new method was developed for the preparation of highly monodisperse isotopically enriched Si-29 silica nanoparticles (29Si-silica NPs) with the purpose of using them as spikes for isotope dilution mass spectrometry (IDMS) quantification of silica NPs with natural isotopic distribution. Si-29 tetraethyl orthosilicate (29Si-TEOS), the silica precur...