Hayato Maeda

• Doctor of Philosophy
• Professor (Associate) at Hirosaki University

Lactococcus lactis subsp. lactis (L. lactis) is a common species of lactic acid bacteria (LAB). There is increasing evidence that probiotic L. lactis ameliorates experimental colitis in mice, whereas few studies have revealed the effects of the paraprobiotics, i.e., inactivated forms of bacteria. L. lactis L8 strain is a newly identified plant-deri...

Phytoestrogens are estrogen-like compounds found in various plants. Menopausal women experience various menopausal symptoms due to reduced estrogen secretion. Ingesting phytoestrogens can alleviate these symptoms. Blackcurrant (Ribes nigrum L., Grossulariaceae) is rich in phytochemicals, including anthocyanins. Blackcurrant extract and anthocyanins...

Apple pomace is the residue left after apples are squeezed. The majority of pomace produced worldwide is produced by the apple manufacturing industry, however, most of the pomace produced by the industry is discarded. Apple pomace contains functional ingredients, such as polyphenols and triterpenoids, and exerts several beneficial effects on human...

Phytoestrogens are estrogen-like molecules found in various plants. Menopausal women experience various menopausal symptoms due to reduced estrogen secretion. The ingestion of phytoestrogens is known to alleviate these symptoms. Blackcurrant ( Ribes nigrum L.) is a fruit rich in phytochemicals, including anthocyanins. Blackcurrant extract (BCE) and...

Ishimozuku ( Sphaerotrichia firma ) is one species of edible brown algae in Japan. The lipids contain rich of fucoxanthin and n-3 unsaturated fatty acids. This study analyzed dietary Ishimozuku lipid components and the effects on fatty liver and intestinal metabolite component production in obese model mice. Fatty acid composition and fucoxanthin c...

Alzheimer’s disease (AD) is a neurodegenerative disease that involves progressive cognitive decline accompanied by synaptic degeneration and impaired neurotransmission. Recent studies revealed that apple pomace, a waste byproduct of the apple processing industry, has beneficial health properties, but its potential to prevent and treat AD has not be...

The present study evaluated the eating quality of uncooked white rice using Raman spectroscopy, operating with a 532 nm excitation beam, in conjunction with multivariate calibration. This analysis was performed to establish correlations between features of the spectra and the results of conventional sensory tests with the aim of establishing calibr...

Background/aim: Fucoxanthin (Fx), a dietary marine xanthophyll, exerts potent anticancer effects in various colorectal cancer (CRC) animal models. However, therapeutic effects of Fx in human cancer tissues remain unclear. A patient-derived xenograft (PDX) mouse model transplanted with cancer tissues from patients is widely accepted as the best pre...

The effects of extraction techniques on the antioxidant and antibacterial activity of stevia (Stevia rebaudiana Bertoni) leaf extracts were assessed. The stevia leaf extracts contained appreciable amounts of total phenolic contents (7.8-21.63 mg GAE/g of dry extract), and total flavonoid contents (31.37-70.41 mg QE/g of dry extract). The EC 50 valu...

Pancreatic cancer (PC) is one of the most fatal cancers, and there is an urgent need to develop new anticancer agents with fewer side effects for the treatment of this condition. A patient-derived xenograft (PDX) mouse model transplanted with cancer tissue from patients is widely accepted as the best preclinical model for evaluating the anticancer...

Reportedly, a relationship exists between intestinal microflora and obesity-related lifestyle diseases. Blautia spp. a major intestinal microbiota, accounts for 3–11% of human intestinal microflora. Epidemiological reports have described that people with more visceral fat have less Blautia hansenii in their intestinal tract irrespective of age or g...

Paraphaeolactones A1, A2, B1, and B2 (1-4, respectively), known arthropsadiol D (5), massariphenone (6) and its positional isomer 7, and massarilactones E (8) and G (9) were isolated from the culture broth of Paraphaeosphaeria sp. KT4192. Although the structural resemblance between 1 and 2 implies that these comprised a diastereomeric pair at the C...

Tomato products containing lycopene can improve the quality of meat products during storage. However, few studies have compared the effects of different tomato products, including tomato paste (PAS), tomato powder (POW), and strained tomato, on meat quality. Herein, under two storage conditions (5 days at 37 °C under fluorescent light and 15 days a...

Fucoxanthinol (FxOH), the main metabolite of the marine carotenoid fucoxanthin, exerts anti-cancer effects. However, fragmentary information is available on the growth-inhibiting effects of FxOH on breast cancer (BC). We investigated the growth-inhibiting effects of FxOH on human BC cells (MCF-7 and MDA-MB-231 cells), and the underlying mechanisms,...

Alzheimer's disease (AD) is associated with a progressive worsening in cognitive function, which is often accompanied by emotional disturbance. Recent studies revealed that anthocyanin‐rich blackcurrant extract (BCE) can impart health benefits, but it is not known whether BCE is useful in the prevention and/or treatment of AD. Here, we examined the...

Background/aim: Fucoxanthinol (FxOH), a marine carotenoid, induces apoptosis and anoikis in human colorectal cancer (CRC) DLD-1 cells via the down-regulation of chloride intracellular channel 4 (CLIC4) expression, a key molecule for apoptosis. However, whether FxOH is susceptible to CLIC4 expression and its regulatory mechanisms in human CRC cells...

Fucoxanthin (Fx) is a critical pigment required for photosynthesis in brown algae and microalgae. Fx is also a dietary marine carotenoid that with potent anticancer activity in vitro and in vivo. Some popular light meals for increased satiety, such as biscuits, cereals, and crackers, are frequently fortified with micronutrients for human health ben...

GCN1 is an evolutionarily-conserved ribosome-binding protein that mediates the amino acid starvation response as well as the ribotoxic stress response. We previously demonstrated that Gcn1 mutant mice lacking the GCN2-binding domain suffer from growth retardation and postnatal lethality via GCN2-independent mechanisms, while Gcn1-null mice die earl...

Fucoxanthin (Fx) is a marine carotenoid with anti-inflammatory and anti-cancer properties in various animal models of carcinogenesis. However, there is currently no information on the effects of Fx in animal models of pancreatic cancer. We investigated the chemopreventive effects of Fx in C57BL/6J mice that received allogenic and orthotopic transpl...

Polyphenols are bioactive compounds found naturally in fruits and vegetables; they are widely used in disease prevention and health maintenance. Polyphenol-rich blackcurrant extract (BCE) exerts beneficial effects on vascular health in menopausal model animals. However, the vasculoprotective effects in diabetes mellitus (DM) and atherosclerotic vas...

Fucoxanthin (Fx) has shown potential cancer chemopreventive functions in a carcinogenic murine azoxymethane/dextran sodium sulfate (AOM/DSS) model. However, the molecular mechanisms based on transcriptome profiles in vivo remain poorly understood. We investigated Fx-dependent alterations of the transcriptome with cancer-associated proteins in color...

We propose a method for predicting the concentration of a functional ingredient, procyanidin, in apple using Raman spectroscopy in combination with multivariate calibration analysis. A regression model was constructed by partial least-squares (PLS) regression using the collected Raman spectra and the procyanidin concentrations measured by high-perf...

Colorectal cancer (CRC), which ranks among the top 10 most prevalent cancers, can obtain a good outcome with appropriate surgery and/or chemotherapy. However, the global numbers of both new cancer cases and death from CRC are expected to increase up to 2030. Diet-induced lifestyle modification is suggested to be effective in reducing the risk of hu...

Background/aim: Fucoxanthinol (FxOH) is a marine carotenoid metabolite with potent anti-cancer activity. However, little is known about the efficacy of FxOH in pancreatic cancer. In the present study, we investigated the inhibitory effect of FxOH on six types of cells cloned from N-nitrosobis(2-oxopropyl)amine (BOP)-induced hamster pancreatic canc...

Paprika Capsicum annuum L. (Solanaceae) contains various carotenoids such as capsanthin, capsorubin, cryptocapsin cucurbitaxanthin A, β-cryptoxanthin, capsanthin epoxide, zeaxanthin, and β-carotene. Especially, capsanthin and capsorubin are characteristic carotenoid in paprika. They show strong antioxidative effect. Furthermore, these carotenoids s...

Background/aim: Fucoxanthinol (FxOH), a predominant metabolite from fucoxanthin (Fx), can exert potential anti-cancer effects in various cancers. However, limited data are available on the effect of FxOH or Fx on pancreatic cancer. The present study investigated the effect of FxOH on a cell line derived from pancreatic cancer tissue developed in P...

Background/aim: A xanthophyll of fucoxanthin (Fx) is a potential chemopreventive agent. Familial adenomatous polyposis (FAP) is an inherited disease that is associated with a high risk of developing colorectal cancer. However, it remains unclear whether Fx can modify colorectal tumorigenesis in ApcMin/+ mice, a model mouse for human FAP. Material...

Fucoxanthin and its metabolite fucoxanthinol (FxOH), highly polar xanthophylls, exert strong anticancer effects against many cancer cell types. However, the effects of Fx and FxOH on pancreatic cancer, a high mortality cancer, remain unclear. We herein investigated whether FxOH induces apoptosis in human pancreatic cancer cells. FxOH (5.0 μmol/L) s...

Estrogen exerts cardioprotective effects in menopausal women. Phytoestrogens are plant-derived substances exhibiting estrogenic activity that could beneficially affect vascular health. We previously demonstrated that blackcurrant (Ribes nigrum L.) extract (BCE) treatment exerted beneficial effects on vascular health via phytoestrogenic activity in...

Background: Ethnobotanical survey in the rural villages in Nepal revealed that the fruits of Rhus chinensis Mill. have been using for the treatment of diarrhea and dysentery. Objective: To evaluate antimicrobial and antioxidant effects, and identification of chemical constituents in the fruits of R. chinensis. Materials and Methods: Phytochemical s...

Background/aim: Fucoxanthin (Fx) is a potent anticancer carotenoid, demonstrated by mouse cancer models. We recently showed the decrease of salivary glycine could represent an attenuation of tumor microenvironment (TME) formation in an azoxymethane/dextran sodium sulfate (AOM/DSS) colon cancer mouse model. However, it remains unclear whether the s...

This study examined the effects of dietary Alaska pollock (Theragra chalcogramma) protein (APP) on nonalcoholic fatty liver disease (NAFLD) and gut microbiota in leptin-deficient ob/ob mice. Mice were fed with high-fat diet containing either casein or APP for six weeks. Then, lipid accumulation and mRNA expression levels in the liver and the compos...

The Maillard reaction intermediates and related phytochemicals in garlic (Allium sativum L.), which was heated for various lengths of time, using X-band (9 GHz) electron paramagnetic resonance (EPR) and high performance liquid chromatography (HPLC) were investigated. Non-spin-trap and non-destructive EPR detected the total reaction intermediates (r...

Fucoxanthin (Fx), a marine carotenoid found in edible brown algae, is well known for having anti-cancer properties. The gut microbiota has been demonstrated as a hallmark for colorectal cancer (CRC) progression in both humans and rodents. However, it remains unclear whether the gut microbiota is associated with the anti-cancer effect of Fx. We inve...

Fucoxanthin is a marine xanthophyll found in edible brown algae, and a metabolite, fucoxanthinol (FxOH), possesses a potent apoptosis inducing effect in many cancer cells. Chloride intracellular channel 4 (CLIC4) is a member of the CLIC family that plays an important role in cancer development and apoptosis. However, the role of CLIC4 in FxOH-induc...

Estrogen is involved in lipid metabolism. Menopausal women with low estrogen secretion usually gain weight and develop steatosis associated with abnormal lipid metabolism. A previous study showed that blackcurrant (Ribes nigrum L.) extract (BCE) had phytoestrogen activity. In this study, we examined whether BCE improved lipid metabolism abnormaliti...

The purpose of this study was to confirm inosine monophosphate (IMP) generation and to clarify the decomposition pathway of adenosine monophosphate (AMP) by investigating the properties of AMP, IMP, and adenosine (AdR) decomposition enzymes in Japanese scallop (Patinopecten yessoensis). The results showed that IMP accumulated due to AMP decompositi...

Seiridium sp. KT3957 produces a secondary metabolite giving identical 1H and 13C NMR spectra to those of seiricardine B (2), a bicyclic sesquiterpene monoalcohol isolated from Seiridium cupressi. An independent spectral analysis suggested that the isolated compound contains one extra H2O unit compared with the original molecular formula, which was...

Seiridiasteriscane A (1), pestalotiopsins L-N (2–4), and 2-O-deacetylpestalotiopsin A (5) were isolated along with known pestalotiopsins A (7), B (6), and fuscoatrol A (8) from Seiridium sp. KT3957. Their relative structures were elucidated by extensive NMR analysis and verified using DFT (density functional theory) chemical shift calculations. The...

Anthocyanins possess high antioxidant activity and are the major group of polyphenols in blackcurrant, a regional specialty in Aomori prefecture. In this study, we investigated the effect of black currant extracts on cognitive and emotional abnormalities in the senescence-accelerated mouse prone 8 (SAMP8). Four month-old SAMP8 mice were fed a basal...

Anthocyanins possess high antioxidant activity and are the major group of polyphenols in blackcurrant, a regional specialty in Aomori prefecture. In this study, we investigated the effect of black currant extracts on cognitive and emotional abnormalities in the senescence-accelerated mouse prone 8 (SAMP8). Four month-old SAMP8 mice were fed a basal...

Fucoxanthin (Fx), abundantly contained in edible brown algae, is a carotenoid with strong anti-cancer potential. Anoikis is an anchor-dependent apoptosis particularly related to integrin signaling, and a target for cancer preventive strategies. We recently demonstrated that Fx prevented colon cancer in azoxymethane-dextrane sodium sulfate (AOM/DSS)...

Fucoxanthin (Fx) is a major xanthophyll in Undaria pinnatifida (wakame) and is one of the potent carotenoids against cancer development. However, the cancer preventive function of high fucoxanthin (Fx-high) wakame itself has not been well investigated. Therefore, we cultivated Fx-high wakame and investigated the effect of this alga on a AOM/DSS car...

Background: Blackcurrant anthocyanin (BCA) is expected to repair endothelial dysfunction, but it remains unclear whether beneficial effects are present in young healthy persons. This study examines whether supplements containing blackcurrant anthocyanin improve endothelial function and peripheral temperature in young smokers. Methods: Young, health...

Arundifungin (1) has been reported as a potent antifungal agent against Candida and Aspergillus spp; however, only its planar structure has been disclosed. This paper describes the assignment of the relative and absolute configuration of 1, which includes (a) determination of the relative configuration of the ABCD polycyclic ring moiety on the basi...

To examine the role of RNA silencing in plant defenses against viroids, a Dicer-like 2 and 4 (DCL2&4)–double knockdown transgenic tomato plant line, 72E, was created. The expression of endogenous SlDCL2s and SlDCL4 in line 72E decreased to about a half that of the empty cassette line, EC. When challenged with potato spindle tuber viroid (PSTVd), li...

Six novel neomacrophorin and four novel premacrophorin congeners were isolated from the fungus Trichoderma sp. 1212-03. Their planar structures and relative configurations were determined by conventional NMR and mass spectrometry techniques. The electronic circular dichroism (ECD)spectral comparisons with known neomacrophorins were effective for el...

Phytoestrogens are plant-derived chemicals that are found in many foods and have estrogenic activity. We previously showed that blackcurrant extract (BCE) and anthocyanins have phytoestrogenic activity mediated via estrogen receptors (ERs), and anthocyanins may improve vascular function. BCE contains high levels of anthocyanins, but their health-pr...

Background/ Aim: A high polar xanthophyll of Fucoxanthin (Fx) is abundantly contained in edible brown algae, and it has chemopreventive effects in mouse cancer models, however, the underlying mechanisms of these effects are not well understood. Thus, we aimed to investigate the effects of Fx on the tumor microenvironment in cancer model mice. Mate...

Fucoxanthin (Fx) and its biotransformed fucoxanthinol (FxOH) present strong anti-cancer effects in vitro and in vivo, however, the underlying mechanisms are not well known. We recently demonstrated that FxOH could induce anoikis-like cells in human colorectal cancer (CRC) cells. Thus, we developed molecular hallmarks for anoikis in vitro, and to co...

The novel trichothecene 12-deoxytrichodermin (3) was isolated from the fungus Trichoderma sp. 1212-03, and included with other known natural trichothecenes in a structure-activity relationship investigation against a human colon cancer cell line (COLO201) and filamentous fungus Cochliobolus miyabeanus. This revealed that the 12-epoxide functionalit...

Fucoxanthin and its major metabolite, fucoxanthinol, have potent anticancer properties in carcinogenic model mice and against cancer cells. Evidence has accumulated regarding the diagnostic potential of biological metabolites as invasive and noninvasive obtainable approaches. We recently demonstrated that glycine was an effective predictor of the s...

To examine the role of RNA silencing in defense against viroid, a Dicer-like 2 and 4 (DCL2&4)—double knockdown transgenic tomato line 72E was created. The expression of endogenous DCL2 and DCL4 in line 72E decreased to about a half of the empty cassette line EC. When challenged with potato spindle tuber viroid (PSTVd), 72E allowed significantly hig...

Main conclusion: In soybean, occurrence of, or tolerance to, seed cracking under low temperatures may be related to the presence or absence, respectively, of proanthocyanidin accumulation in the seed coat dorsal region. Soybean seeds sometimes undergo cracking during low temperatures in summer. In this study, we focused on the occurrence and toler...

Fucoxanthin is a specific carotenoid in brown seaweeds with remarkable biological properties. Ishimozuku (Sphaerotrichia divaricata), an edible brown alga from northern Japan, has morphology that is almost identical to that of Okinawa-mozuku (Cladosiphon okamuranus) harvested off Okinawa, Japan. However, because of Ishimozuku’s lower availability c...

Fucoxanthin and its major metabolite, fucoxanthinol, have potent anti-cancer properties in carcinogenic model mice and against cancer cells. Evidence has accumulated regarding the diagnostic potential of biological metabolites as invasive and non-invasive obtainable approaches. We recently demonstrated that glycine was an effective predictor of the...

Cyclohelminthols Y1-Y4 (1-4) were isolated from the culture broth of Helminthosporium velutinum yone96. These compounds are diastereomers to each other featuring 3-azabicyclo[3.1.0]hexane-6-spirocyclopentane linked with a cyclopentanespirocyclopropane framework. Their planar structures were established via the comparison of their spectra with the s...

Fucoxanthinol (FxOH) is a strong anticancer metabolite of fucoxanthin that accumulates in abundance in edible brown algae and promises human health benefits. FxOH has been shown to suppress tumorigenicity and sphere formation in human colorectal cancer stem cell (CCSC)-like spheroids (colonospheres, Csps). In the present study, we aimed to clarify...

Blackcurrants (Ribes nigrum L.) contain high levels of anthocyanin polyphenols, which have beneficial effects on health, owing to their antioxidant and anticarcinogenic properties. Phytoestrogens are plant-derived substances with estrogenic activity, which could have beneficial effects on the skin. Estradiol secretion decreases during menopause, re...

Background/aim: Fucoxanthinol (FxOH), a metabolite of fucoxanthin, is known to inhibit tumorigenicity of human colorectal cancer stem cells (CCSCs) and their sphere formation. Hypoxic conditions and hypoxia-inducible factors (HIFs) are essential to maintain the stemness of CCSCs. We investigated effects of FxOH on sphere formation, intercellular e...

Fifteen crossbred (Yorkshire×Duroc×Landrace) castrated male finishing pigs were used to evaluate differences in the growth performance, meat quality, and serum metabolites associated with mixed silages prepared with various proportions of fresh apple pomace (FAP). A basal feed consisting primarily of brown rice was used as the control. For the diff...

Phytoestrogens are plant compounds with estrogenic effects found in many foods. We have previously reported phytoestrogen activity of blackcurrant anthocyanins (cyanidin-3-glucoside, cyanidin-3-rutinoside, delphinidin-3-glucoside, and delphinidin-3-rutinoside) via the estrogen receptor (ER)α. In this study, we investigated the participation of ERβ...

Fucoxanthin (Fx), one of the major xanthophylls in brown algae, is known to be effective for colorectal cancer (CRC) chemoprevention through inhibiting cell growth, cell cycle and caspase activation. Recently, we observed fucoxanthinol (FuOH), an anti-cancer active metabolite of Fx, treatment of human CRC cells resulted in plenty of living floating...

Blackcurrants (Ribes nigrum L., Grossulariaceae) possess a high content of anthocyanin polyphenols, which have been demonstrated to exhibit beneficial effects on health due to their antioxidant and anticarcinogenic prope-rties. The present study investigated novel functions of anthocyanin‑rich blackcurrant extracts (BCEs) in a healthy mammary epith...

Fish muscles are classified into white and red muscles, and the chemical composition of the two fish muscles have many differences. Few reports have assessed the health-promoting functions of white fish muscle proteins (WFP) and red fish muscle proteins (RFP). We therefore evaluated the mechanisms underlying the alteration of lipid profiles and cho...

Fucoxanthinol (FuOH), an intestinal metabolite form of fucoxanthin (Fx) isolated from marine algae, is known to possess multiple health benefits, such as prevention of human cancer. However, there is little available information about the effects of FuOH on colorectal cancer stem cells (CCSCs) and their contribution to drug resistance, tumorigenesi...

Helminthosporium velutinum yone96 produces cyclohelminthol X (1), a unique hexa-substituted spirocyclopropane. Although its molecular formula and NMR spectral data resemble those of AD0157, being isolated from marine fungus Paraconiothyrium sp. HL-78-gCHSP3-B005, our detailed analyses disclosed totally different structure. Chemical shift calculatio...

We investigated the location and distribution of paramagnetic species in black and white rice using electron paramagnetic resonance (EPR), X-band (9 GHz) EPR imaging (EPRI), and HPLC. EPR primarily detected two paramagnetic species in black rice, which were identified as a stable radical and Mn²⁺ species, based on the g values and hyperfine compone...

Neomacrophorin X (1) was isolated from Trichoderma sp. 1212-03. Heteronuclear multiple bond correlation (HMBC) spectral analysis indicated a unique [4.4.3]propellane framework, which was verified by the (1)H and (13)C chemical shift calculations based on density functional theory (DFT) and subsequent comparison with experimental data obtained in CD...

Tuna muscle consists of light and dark muscle in approximately equal proportions. However, besides for the light muscle of tuna, cod, sardine, and salmon, few researches have assessed the health-promoting functions of fish protein. Therefore, we evaluated the mechanisms underlying the alteration of lipid storage and cholesterol metabolism following...

Main conclusion: Defective cracking frequently occurs in buff-pigmented soybean seed coats, where proanthocyanidins accumulate and lignin is deposited, suggesting that proanthocyanidins and/or lignin may change physical properties and lead to defective cracking. In the seed production of many yellow soybean (Glycine max) cultivars, very low percen...

We investigated the location and distribution of paramagnetic species in dry black, brown, and yellow (normal) soybean seeds using electron paramagnetic resonance (EPR), X-band (9 GHz) EPR imaging (EPRI), and HPLC. EPR primarily detected two paramagnetic species in black soybean. These two different radical species were assigned as stable organic r...

No abstract is available for this article.

isolation, structure determination and biological activities

isolation and structure elucidation of trans- and cis-homojesterones

Homopetasinic acid (1) was isolated from fungi of the Diaporthe sp. strain 1308-05. NMR spectroscopic structural analysis revealed a petasol (3) substructure and a (4E,6E)-7-carboxy-3-hydroxy-2-methylhepta-4,6-dienoate side chain. The absolute configuration of the petasol moiety was established by the specific rotation value after basic hydrolysis....

Cyclohelminthols I–V (1–4) were isolated from Helminthosporium velutinum yone96. The positions of the carbons attached to chlorine atoms in 2–4 were determined by signal splitting due to isotopes in high resolution fully proton decoupled 13C NMR spectra. Although these natural products gave poor electron circular dichroism (ECD) spectrum because of...

isolation and structure elucidation of Achaetolide-II (I)

trans- and cis-Homojesterone (1 and 2, respectively), vinylogous analogs of jesterone, were isolated from Helminthosporium velutinum TS28. The relative configurational relationship between H-6 and H-7 was determined by comparing their 3JH-6/H-7 values with those of jesterones in literature, as well as with structurally related natural products. The...

Scope: Blackcurrants (Ribes nigrum L., Grossulariaceae) contain high amounts of anthocyanin polyphenols, which have antioxidant and anti-carcinogenic health benefits. This study analyzed the potential phytoestrogenic effects of blackcurrant extract (BCE) in breast cancer (MCF-7) and human endometrial cancer (Ishikawa) cell lines that over-express...

The effects of calcium concentrations in the growth medium on oxalate content of leaf blades, petioles and corms and the involvement of guttation in the regulation of oxalate homeostasis were investigated in eddo (Colocasia esculenta (L.) Schott var. antiquorum Hubbard & Rehder). The plants were grown hydroponically in solutions containing 0 mM cal...

Fucoxanthin (Fx) is a marine carotenoid found in edible brown seaweeds. We previously reported that dietary Fx metabolite into fucoxanthinol (FxOH), attenuates the weight gain of white adipose tissue of diabetic/obese KK-Ay mice. In this study, to evaluate anti-diabetic effects of Fx, we investigated improving the effect of insulin resistance on th...

A C3-O-acylated analogue of achaetolide (2) was isolated from phytopathogenic fungus Helminthosporium velutinum TS28. The relative configuration involving the C3-O-acyl side chain was determined by NMR spectroscopic analysis combined with conformational analysis computer-assisted chemical shift calculations. The absolute configuration was establish...

The structures of epoxyroussoenone (1) and epoxyroussoedione (3) isolated from a culture broth of Roussoella japanensis KT1651 were determined. Although NMR spectra provided insufficient structural information, computation of the theoretical chemical shifts with DFT EDF2/6-31G* enabled us to elucidate not only the planar structure, but also the rel...