Hassane Oudadesse

Université de Rennes 1 | UR1 · ISCR UMR CNRS 6226

The majority of studies have focused on the industrial exploitation of marine fisheries waste through the production of natural bioactive bioploymeres such as chitin and chitosan. However, in recent years, beetles are increasingly attracting the interest of scientists as a source of chitin and chitosan for the preparation of hydrogels for sustainab...

The present work studied the physicochemical and biological properties of fish scales composed of natural carbonated hydroxyapatite, called SS-CaPx (where x represents sample indexation). Fish scales and mainly European sardine ( Sardina pilchardus ) scales were used, their behavior was compared with those of healthy rat bone (HRB) and synthetic hy...

Derived Hench bioactive glass (BaG) containing boron (B) is explored in this work as it plays an important role in bone development and regeneration. B was also found to enhance BaG dissociation. However, it is only possible to incorporate a limited amount of B. To increase the amount of B in BaG, bioactive borosilicate glasses (BaG-Bx) were fabric...

This work aims to develop an injectable and antibacterial composite cement for bone substitution and prevention/treatment of bone infections. This cement is composed of calcium phosphate, calcium carbonate, bioactive glass, sodium alginate, and ciprofloxacin. The effect of ciprofloxacin on the microstructure, chemical composition, setting propertie...

Three-dimensional hydroxyapatite-chitosan (HA-CS) composites loaded with ciprofloxacin antibiotic (HA–CS–CIP) were formulated using the in situ and the solid-liquid method coupled with the freeze-drying process. The interaction of the HA-CS composite powder and ciprofloxacin antibiotic (CIP) was investigated by batch adsorption essays. The kinetic...

The modification of porosity and surface silanization are efficient methods to improve physicochemical and biological properties of bioactive glass. For sol-gel-derived glasses, the use of surfactant in the synthesis medium allows to pattern porosity and may significantly impact their in vitro bioactivity. Additionally, surface functionalization by...

This study reports for the first time the effect of particle size of bioactive glass 46S6 on the physico-chemical and mechanical properties of carbonated apatite cement (CPC) composed of brushite (CaHPO4. H2O) and vaterite (CaCO3). Three particle size ranges were investigated: coarse (BGc: 100–200μm), medium (BGm: 40–100μm), and fine (BGf<40 μm). F...

Porous bioglass monoliths have been processed by hydrothermal hot pressing (HyHP) from sol-gel and melt-derived bioglass powders of composition (in mol %): SiO2–CaO–P2O5 (55.0–40.0-5.0) and SiO2–CaO–Na2O–P2O5 (47.2–26.4-23.8–2.6), respectively. An open porosity of >70% ever reached in 3D structures is reported for monoliths issued from sol-gel powd...

This work investigates the bioactivity of novel silver-doped (BG-Ag) and gold-doped (BG-Au) quaternary 46S6 bioactive glasses synthesized via a semi-solid-state technique. A pseudo-second-order kinetic model successfully predicted the in vitro uptake kinetic profiles of the initial ion-exchange release of Ca in simulated body fluid, the subsequent...

with the presence of ciprofloxacin which is confirmed by histopathological analysis. Several physicochemical techniques showed a rapid reduction in Si and Na in one hand and an accelerator rise in Ca and P ions concentrations in other hand in BG(M)-PVA than in the BG(M)-PVA-20Cip. Therefore, the incorporation of ciprofloxacin in BG(M)-PVA is charac...

The toxic effect of imidacloprid (IMI)-induced osteoporosis and the protective effect of Urtica urens L. (UU), dwarf nettle was investigated against this toxicity in female rats. Rats were divided into control group, 2 groups treated with IMI at 50 and 300 mg/kg/day, 1 group treated with 100 mg/kg/day of UU and 2 groups co-treated with IMI (50 and...

Aims: This study aimed to investigate the potential effects of a synthetic apatite (carbonated hydroxyapatite) on the detoxification of a group of male "Wistar" rats exposed to nickel chloride. Methods: Toxicity was evaluated by rats bioassay of nickel chloride. Wistar rats received daily this metal by gavage for 7 days (4 mg / ml nickel chloride /...

Aims: This study aimed to investigate the potential effects of a synthetic apatite (carbonated hydroxyapatite) on the detoxification of a group of male "Wistar" rats exposed to nickel chloride. Methods: Toxicity was evaluated by rats bioassay of nickel chloride. Wistar rats received daily this metal by gavage for 7 days (4 mg / ml nickel chloride /...

Orthopedic devices are used in pathologic disorder as an adjunct to bone grafts to provide immediate structural stability. Unfortunately, the use of metallic devices has some complications. This study aimed to characterize the oxidative stress biomarker and the anti-oxidant enzyme profiles during bone regeneration. New Zealand White rabbits were di...

Aims: This study aimed to investigate the potential effects of a synthetic apatite (carbonated hydroxyapatite) on the detoxification of a group of male "Wistar" rats exposed to nickel chloride. Methods: Toxicity was evaluated by rats bioassay of nickel chloride. Wistar rats received daily this metal by gavage for 7 days (4 mg / ml nickel chloride /...

The performance of the porous glass ceramic doped with 10 % wt Zinc and 2% wt TiN (46S6-10Zn) ,in the restoration of critical diaphyseal bone defect, was evaluated by several physicochemical methods and histological studies. The critical defect in rabbits was created and then filled with 46S6-10Zn. At different periods after implementation, animals...

Bone tissue engineering is gaining popularity as alternative method for treatment of osseous defects. A number of biodegradable polymers have been explored for tissue engineering purposes. A new family of biodegradable polymer/bioactive glass composite materials has been designed to be used in bone regeneration approaches. In this work, hybrid scaf...

The toxic effect of imidacloprid (IMI)-induced osteoporosis and the protective effect of Urtica urens L. (UU), dwarf nettle was investigated against this toxicity in female rats. Rats were divided into control group, 2 groups treated with IMI at 50 and 300 mg/kg/day, 1 group treated with 100 mg/kg/day of UU and 2 groups co-treated with IMI (50 and...

The ovariectomized (OVX) rat model is well established in investigations of osteoporosis and anti-osteoporotic therapies. Fourier transform infrared (FTIR) spectroscopy facilitates characterization of bone quality which is a key determinant of bone strength. FTIR has been used to evaluate the changes occurring in the mineral composition of lumbar v...

Calcium phosphate cements (CPC) have been widely investigated as bone substitutes, owing to their attractive features in terms of physicochemical and biocompatibility properties. However, the clinical applicability of this group of biomaterials is still critically limited by its poor strength and rheological properties in terms of injectability and...

A composite based on hydroxyapatite (HA) and chitosan (CS) combined with ciprofloxacin (CIP) was formulated by the solid–liquid mixing method. The optimization of the solid to the liquid ratio and the use of chitosan in a small amount (≤5 wt%) promoted the preparation of stable and rigid monoliths. A synergistic effect of CS and CIP contents on the...

Monoammonium phosphate is a commonly used fertilizer for better crop production. However, its repeated application causes the accumulation of harmful metals levels in plants and soil over time. Here, the impact of the purification of monoammonium phosphate fertilizer on vegetative growth and yield of tomato plants (Lycopersicon esculentum L.), and...

Three-dimensional hydroxyapatite-chitosan (HA-CS) composites were formulated via solid-liquid technic and freeze-drying. The prepared composites had an apatitic nature, which was demonstrated by X-ray diffraction and Infrared spectroscopy analyses. The impact of the solid/liquid (S/L) ratio and the content and the molecular weight of the polymer on...

Bone tissue engineering is gaining popularity as alternative method for treatment of osseous defects. A number of biodegradable polymers have been explored for tissue engineering purposes. A new family of biodegradable polymer/bioactive glass composite materials has been designed to be used in bone regeneration approaches. In this work, hybrid scaf...

Tissue engineering has emerged as an alternative approach to create bone tissue by growing cells on 3D scaffolding. The aim of this study was to synthesize a composite glass/chitosan (BG-CH) by using new salt leaching using powder (SLUP) process in order to control the porosity rate and then the chemical reactivity of the final product. SLUP proces...

We investigated the effects of Kalach 360 SL (KL), Glyphosate (G)-based herbicide, on bone tissue in different groups of female Wistar rats. Group 1 (n = 6) received a standard diet and served as a control, groups 2 and 3 (n = 6 each) received 0.07 ml (D1: 126 mg/Kg) and 0.175 ml (D2: 315 mg/Kg) of KL dissolved in the water for 60 days. The plasma...

The damage to normal bone tissue following therapeutic irradiation increases the risk of fracture and bone osteoporosis. Bioactive glasses have been synthesized with improved properties exhibiting promising prospects to restore bone defects and to reduce bone alterations. This study aims in evaluating the performance therapy of nano-bioglass (NBG)....

The present study aimed to enhance the anti-osteoporotic performance of bioactive glass (46S6) through its association with bisphosphonate such as risedronate with amounts of 8, 12, and 20%. Obtained composites have been called 46S6-8RIS, 46S6-12RIS, and 46S6-20RIS, respectively. In vitro and in vivo explorations have been carried out. Bioactive gl...

This research is based on the study of bioactivity kinetic in function of the glass particles size. Bioactive glasses have been elaborated in the ternary system SiO2-CaO-P2O5. Nano bioactive glass and micro bioactive glass have been synthesized by using two different processes. They are destined for use as bone biomaterials. The comparison was focu...

Bioactive glasses have the physical characteristics enabling them to be used in bone tissue engineering applications. However, the exact mechanism of the interactions between the glass surface and environment leading to the transition from the vitreous phase to the crystalline phase remains a subject of study. This work focuses on the growth of a c...

In this study, we report a simple, efficient and green protocol for the synthesis of pyrano[2,3-c]pyrazole derivatives by the use of the natural phosphate K09 as a mild and efficient heterogeneous catalyst. The catalytic efficiency of K09 was compared with other heterogeneous catalysts to determine the best catalyst for said conversion. Easy recove...

Phosphate glasses, with molar compositions 37.5Na2O-25[(1-x)MgO-xNiO]-37.5P2O5 (0 ≤ x ≤ 1), have been prepared using the conventional melt quenching technique. The free nickel glass is colorless while the glasses containing nickel are yellow. The effect of Ni 2+ ions on structural and physico-chemical properties of these glasses has been investigat...

The present research work is an in vivo study that aimed to evaluate the potential role of bioglass–chitosan (BG–CS) and bioglass–chitosan–20%ciprofloxacin (BG–CS–20Cip) in antioxidant profile and osteointegration. These scaffolds were implanted in the defect bone of femoral condyles in ovariectomized rats. The treatment with BG–CS–20Cip has shown...

This research is based on the study of thermal behaviour of bioactive glass in the quaternary system SiO2-CaO-Na2O-P2O5 doped with metallic elements such as Zn and Sr destined for use as bone biomaterial. It encompasses glass transition temperature, crystallization temperature and melting temperature. This work is based on both determination and co...

In this work, we report a simple, efficient and green protocol for the synthesis of dihydropyrimidinones/thiones (products of Biginelli reaction) by the use of white marble as an effective heterogeneous catalyst. Short reaction times, high product yields, simple processing procedure and reusability of the catalyst are the superior characteristics o...

This work is devoted to study the reactivity of the quaternary glass 52S4 (52% SiO2–30% CaO–14% Na2O–4% P2O5 (wt%)), synthesized by sol-gel process versus the treatment temperature. The dried gel was heat treated at 600 and 650 °C and soaked in simulated body fluid (SBF). XRD results confirm the amorphous character of glass treated at 600 °C even t...

The present study aimed to evaluate the effect of bioactive glass as well as the presence of Ciprofloxacin drug (%Cip) into bioactive glass–chitosan composite on the in vivo behavior of these scaffolds. These scaffolds were implanted in the femoral condyl of an ovariectomized rat. The serum and organs (liver and kidney) of the under investigated ra...

Objective: In the present study, we investigate the biological performance of a calcium phosphate ceramics (CPC) bone substitute combined with poly-hydroxybutyrate-co-hydroxyvalerate (PHBV). Materials and Methods: A particulate CPC [45% beta-tricalcium phosphate (β-TCP) and 55% of dihydrated dicalcium phosphate (DCPD)] was incorporated into a biode...

This study investigated the effect of bioglass (melting)-polyvinyl alcohol (BG (M)-PVA) and bioglass (melting)-polyvinyl alcohol-20 %ciprofloxacin (BG(M)-PVA-20Cip) in improving antioxidant activity and regenerating bone capacity. These composites were implanted in femoral condyles of ovariectomized Wistar rats and compared to that of controls grou...

Bioactive glass particle is used in the repair of bone defects. This material undergoes a series of surface in vivo reactions, which leads to osteointegration. We evaluated the effect of the bioactive glass synthesis, sol-gel (BG(S)) versus melting (BG(M)), associated with polyvinyl-alcohol (PVA) on in vivo bioactivity with biochemical parameters,...

Bioactive glasses are indicated for use as bone substitutes in orthopedic or dental surgery because of their high reactivity once in contact with the biological medium. Boron is known as a glass network former and an activator of the glasses bioactivity. In this work, bioactive glass was doped by 5, 10 and 20 wt% of B2O3 according to the melting pr...

The aim of the current work is to study the physicochemical interactions between bisphosphonates molecules, risedronate (RIS) and bioactive glass (46S6) after their association by adsorption phenomenon. To more understand the interaction processes of RIS with the 46S6 surface we have used complementary physicochemical techniques such as Infrared (F...

Mesenchymal stem cells (MSCs) have been the subject of many studies in recent years, ranging from basic science that looks into MSCs properties to studies that aim for developing bioengineered tissues and organs. Adult bone marrow-derived mesenchymal stem cells (BM-MSCs) have been the focus of most studies due to the inherent potential of these cel...

This study reports the in vitro biocompatibility of a composite biomaterial composed of 46S6 bioactive glass in association with chitosan (CH) by using 3D osteoblast culture of SaOS2. The 46S6 and CH composite (46S6-CH) forms small hydroxyapatite crystals on its surface after only three days immersion in the simulated body fluid. For 2D osteoblast...

The performance of the porous glass ceramic doped with 10% wt Zinc and 2% wt TiN (46S6-10Zn),in the restoration of critical diaphyseal bone defect, was evaluated by several physicochemical methods and histological studies. The critical defect in rabbits was created and then filled with 46S6-10Zn. At different periods after implementation, animals w...

Biomaterials for bone reconstruction represent a widely studied area. In this paper, a new method of synthesis of a porous glass–ceramic obtained by thermal treatment is presented. The prepared biomaterial was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and induced couple plasma-optical emission spectroscopy (ICP-O...

Chitosan scaffolds, combined with bioactive glass 46S6, were prepared to serve as gentamicin sulfate delivery in situ systems for bone biomaterials. This work presents a study about the effect of the ratio chitosan/bioactive glass (CH/BG) on the release of gentamicin sulfate and on the bioactivity during in vitro experiments. SEM observations allow...

Background: Synthetic bone graft substitutes such as bioactive glass (BG) material are developed in order to achieve successful bone regeneration. Zn plays an important role in the proper bone growth, development, and maintenance of healthy bones. Aims: This study aims to evaluate in vivo the performance therapy of zinc-doped bioactive glass (BG-Zn...

Background: Synthetic bone graft substitutes such as bioactive glass (BG) material are developed in order to achieve successful bone regeneration. Zn plays an important role in the proper bone growth, development, and maintenance of healthy bones. Aims: This study aims to evaluate in vivo the performance therapy of zinc-doped bioactive glass (BG-Zn...

Heat capacity measurements have been conducted by means of DSC on both crystalline and glassy lithium metaphosphate, from room temperature up to the melting region. The heat capacity of the glass is slightly higher than that of the crystal. Contrary to the crystal, in the neighborhood of T g, C p increases rapidly by 10 J mol−1 K−1 conferring to th...

Three bioactive glasses belonging to the system SiO2-CaO-Na2O-P2O5 elaborated by conventional melt-quenching techniques were doped with silver, copper and copper + zinc. They were characterized using the usual physical methods. Human osteoblast cells Saos-2 and human endothelial cells EAhy926 were used for viability assays and to assess the metalli...

This work describes a quantitative kinetic approach to assess the in-vitro bioactivity of gold-doped hydroxyapatite-polyvinyl alcohol nanocomposites. The surface morphology of the in-situ prepared nanocomposites as characterized by Transmission Electron Microscopy (TEM) revealed a rod-like shape. Differential Thermal Analysis-Thermogravimetric (DTA...

The biomaterials are used for many biomedical applications. The main objective of the present work was to investigate the potential role of Bioglass (Melting)- Polyvinyl alcohol (BG (M)-PVA) and Bioglass (Melting)-Polyvinyl alcohol-20%Ciprofloxacin (BG (M)-PVA-20Cip) in regenerative bone capacity. These composites were implanted in the femoral cond...

CaSiO3 ceramics have been proposed as a medical material for artificial bone and dental root, since they proved to be bioactive and biocompatible. In this study, CaSiO3 powders were synthesized by mixing of Calcium oxide (CaO) and (SiO2) for 3 h, and subsequent calcination of the mixture at 710 °C for 2 h. The compacts were conventionally sintered...

Radiation therapy contributes to a significant increase in bone osteoporosis and skin loss. Various natural health products might be beneficial to reduce bone and skin alterations. Curcumin (CUR) medicines derived from natural plants have played an important role in health care. This study aims at synthesizing and evaluating the performance therapy...

CaSiO3 ceramics have been proposed as a medical material for artificial bone and dental root. In this work, CaSiO3 powders were synthesized by mixture of (CaO) and (SiO2). The samples were sintered at 1,150°C for 2 h. Moreover, amounts of B2O3 (5.0 wt%) have been added. The in vitro bioactivity of both CaSiO3 and B2O3-CaSiO3 ceramics was investigat...

Aims: This study aimed to investigate the potential effects of a synthetic apatite (carbonated hydroxyapatite) on the detoxification of a group of male "Wistar" rats exposed to nickel chloride. Methods: Toxicity was evaluated by rats' bioassay of nickel chloride. Wistar rats received this metal daily by gavage for seven days (4 mg/ml nickel chlo...

Bioactive glasses are indicated for use as bone substitutes in orthopedic or dental surgery because of their high reactivity once in contact with the biological medium. Boron is known as a glass network former and an activator of the glasses bioactivity. In this work, bioactive glass was doped by 5, 10 and 20 wt% of B 2 O 3 according to the melting...

Critical-sized bone defects have in many cases posed challenges to the current gold standard treatments. Bioactive glasses are reported to be able to stimulate more bone regeneration than other bioactive ceramics; however, the difficulty in producing porous scaffolds made of bioactive glasses has limited their extensive use in bone regeneration. On...

The main objective of the present work was to investigate the effect of surfactant chain length on the structure, porosity and bioactivity of 92S6 (92% SiO2, 6% CaO, and 2% P2O5 mol%) mesoporous sol-gel glasses. The aim was to provide a basis for controlling the porosity of the glass to obtain a control of bioactive behavior. A series of mesoporous...