Hassan Y. Aboul-Enein

• PhD ;DSc
• Professor at National Research Centre

Enantioseparation of five β-adrenergic blockers was studied using two mobile phases on a cellulose tris(3‐chloro‐4‐methylphenylcarbamate) (Lux-Cellulose-2) chiral column in normal phase mode. The first mobile phase composed of n-hexane: ethanol: diethylamine 60: 40: 0.1 by volume has successfully resolved the chromatographic peaks of three pairs of...

Mebendazole (MBZ) is a wide-spectrum antihelmintic drug and, more recently, has proven to beactive in various types of cancer. In both cases, its efficacy is limited by insufficient bioavailability due to itsvery low solubility and inadequate release kinetics. This paper presents the preparation and in vitro availabili-ty of solid dispersions of MB...

Objective: The sample preparation step is the initial step in pharmaceutical analysis. Ultrafiltration is a well-known technique used in the food and pharmaceutical industry. However, it has been rarely used for quantification of the plasma concentration of active pharmaceutical ingredients (APIs) when it is compared with some other well-known samp...

Non-biodegradable plastics are an unresolved issue in solid waste management as recycling them is expensive. This study focused on formulating a biodegradable gellan gum (GG) membrane embedded with multiwalled carbon nanotubes (MWCNTs) as the adsorbent in the micro-solid phase extraction (µ-SPE) of selected polycyclic aromatic hydrocarbons (PAHs),...

Glutathione S- transferase P1 (GST- P1) is considered as a detoxification enzyme and can be upregulated in several cancers. Therefore, qualification and/or quantification of GST-P1 in biological fluids can be noteworthy in cancer diagnostic and/or prognostic methods. Whereas costly immunoassays methods are routinely used for clinical analysis, long...

The development of chiral selectors for the separation and analysis of chiral molecules has been an evolving process happening over three decades, since the introduction of the first chiral stationary phase (CSP) in 1938. The main impetus for designing new chiral selectors is to get to most promising one which has a broad chiral recognition propert...

Despite organophosphorus pesticides (OPPs) benefits in controlling vector-borne diseases and noxious insects, the bioaccumulation and persistence in the soil system may metamorphose into new substances which could pose a serious threat to the ecosystems and human health. The generally low levels of OPPs residues and often the complexity of the soil...

Despite organophosphorus pesticides (OPPs) benefits in controlling vector-borne diseases and noxious insects, the bioaccumulation and persistence in the soil system may metamorphose into new substances which could pose a serious threat to the ecosystems and human health. The generally low levels of OPPs residues and often the complexity of the soil...

Warionia saharae Benth & Coss (W.s) (Asteraceae) is a monospecific genus endemic to Algeria and Morocco. Its leaves are used in their traditional medicine, such as gastrointestinal and inflammatory diseases; for instance, rheumatoid arthritis treatment. In this work, our team investigated the anti-inflammatory and analgesic effects of essential oil...

Most of the alkaloids are chiral compounds and are clinically administered as the racemic mixture, even though its enantiomers have been known to exert different pharmacological activity. The determination of the enantiomeric composition of alkaloid-containing plants is subject to severe attention from pharmacological and toxicological points of vi...

Objective Linezolid is a significant antibiotic used against severe infections initiated by multi-resistant bacterial pathogens. Method Linezolid extraction from plasma is obtained using methanol. Chromatographic separation is achieved isocratically on a C18 column [Zorbax C18, 5 µm particle size, 150 mm ˟ 4.6 mm] making use of a mobile phase of a...

A sample preparation method based on modified Quick, Easy, Cheap Effective, Rugged, and Safe (QuEChERS) and GC–MS method was developed and validated for identification and quantification of amphetamine (AP), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxymethamphetamine (MDMA) in human urine. Several derivatization...

Background Bisphenols A (BPA), bisphenol S (BPS), and bisphenol F (BPF) are manufactured chemicals found in food packaging, drink containers, lotions, toys, plastic PVC flooring and water pipes. The aim of this work is to develop simple, rapid and reliable high-performance liquid chromatography with fluorescence detector (HPLC-FLD) method for simul...

This cover picture describes a fast and simple capillary electrophoresis method for the simultaneous analysis of caffeine, vanillin, and ethyl vanillin in beverages. The optimal separation conditions were 25 mM phosphate buffer at pH 8.5, containing 10% acetonitrile and detected at 200 nm. Employing the optimum conditions, the three compounds were...

Oral anticoagulants are widely used for treating and preventing thromboembolic diseases. The most common oral anticoagulatory agents are vitamin K antagonists which belong to the coumarin family, their target is to inhibit the enzyme vitamin K epoxide reductase, so they block the hepatic synthesis of the active, reduced form of vitamin K necessary...

Purpose of Review Adsorbent-based microextraction is a dynamic and simple sample preparation that allows for simultaneous extraction and enrichment of targeted analytes from a sample matrix. Its versatility, efficiency, and compliance with green analysis have contributed to its popularity against conventional solid-phase extraction. This review foc...

2D-HPLC is an important development in chiral separations due to its excellent capability to resolve the enantiomers. This article describes the latest update in the chiral resolution of racemates using 2D-HPLC. The various aspects of the chiral resolution such as validation, optimization, and applications are discussed in both biological and envir...

In this work, enantiomeric separation of a drug combination of two chiral drugs, namely, atenolol and chlorthalidone, is described. Prior investigation of the effect of different variables on the resolution of the enantiomers' peaks and the total run time represented by the retention time of the last eluted peak was conducted using face‐centered co...

Fast and simple capillary electrophoresis method was proposed for the simultaneous determination of caffeine, vanillin, and ethyl vanillin in beverages. The optimal separation conditions were 25 mM phosphate buffer at pH 8.5, containing 10% (v/v) acetonitrile and UV‐absorption detection at the wavelength of 200 nm. Employing the optimum conditions,...

The concentration levels of some important pesticides were evaluated in soil samples from different areas of the North of Iran (Golestan province) and to indicate the possible sources and risks of contamination. A multiresidue analytical procedure using the QuEChERS approach was developed to extract and measure 12 pesticide residues from ten differ...

In this study, a new type of multifunctional material PANI/NiO/MnO2 nanocomposite was first time prepared by chemical polymerisation from aniline monomer in the presence of metal oxides (NiO and MnO2) and an oxidant (ammonium persulfate) in acidic aqueous solution for the elimination of methyl orange (MO) dye from water. Herein, the PANI/NiO/MnO2 n...

Synthesis of three chiral 4,5-Di methyl Δ4 N-phenyl N-aryl imidazole-2-thione derivatives was obtained by the condensation reaction of thiourea derivatives with α-hydroxy ketone. The structure of these compounds has been characterized by using spectroscopic methods (UV, IR, 1 H NMR, and 13C NMR). The 4,5-Di methyl Δ4 N-phenyl N-aryl imidazole-2-thi...

Chiral molecules in relation to particular biological roles are stereoselective. Enantiomers differ significantly in their biochemical responses in biological environment. Despite the current advancement in drug discovery and pharmaceutical biotechnology, the chiral separation of some racemic mixtures continues to be one of the greatest challenges,...

A thionation reaction was performed on some chiral flavanones using Lawesson's reagent (LR) and leads to the formation of new chiral thiocarbonyl flavanes. LR in this thionation reaction with Hesperetin and Naringenin gives new flavan-4-thiones with yields ranged between 41 and 52%. Based on the Wittig reaction principle, LR is currently the most w...

Alkaloids are distributed in plant kingdom and play important role in protection, germination as well as plant growth stimulants. Most of them are chiral compounds and are clinically administered as the racemic mixture, even though its enantiomers have been known to exert different pharmacological activity. Liquid chromatography using chiral statio...

Even after almost 30 years of documented investigations in the field of chiral environmental pollutants, enantiomers are often still considered as two halves of one chiral pollutant, although they behave differently in homo-chiral biological environments. Thus, the below citations still need to be highlighted in order to initiate a special sensitiv...

Nowadays, the environment is being continuously contaminated by different pollutants, including many organic pollutants that are chiral. It is well known that several toxic pollutants enter into the human body via the food chain. Well-known toxic chiral contaminants include pesticides, plasticisers phenols, biphenyls, polycyclic aromatic hydrocarbo...

The analytical separation technologies with most applications to enantiomer-selective chromatographic separations are high-performance liquid chromatography (HPLC) and the more advanced ultrahigh-performance liquid chromatography (UHPLC) based on stationary phases with particles <3 μm of size. Enhanced chromatographic resolutions by applying ultraf...

In a non-chiral environment, the enantiomers of a racemate possess the same physical and chemical properties. By contrast, metabolic and regulatory processes mediated by biological systems are sensitive to stereochemistry and different responses can often be observed when comparing the activities of a pair of enantiomers (Ali and Aboul-Enein 2004;...

As outlined earlier, the notorious lack of well-validated standards of highest optical purity is still a considerable drawback for reliable peak assignments and quantitation (Landagaray et al. 2016; Naile et al. 2016; Hasan et al. 2017; Ulrich et al. 2018). Furthermore, enantioselective analyses today are an integrated part of toxicological and mec...

For the quantification of trace amounts of pollutants, complex validation protocols for method validation and quality control (QC) are required today in order to provide reliable results suitable for further statistical treatment in national and international surveys and pollutant monitoring programmes. National and international protocols today ar...

During the past two decades and after the first edition of this monograph was published in 2001, tremendous progress has been made with respect to sensitivity and selectivity in scientific technology for ultra-trace analysis. Today, chromatographic separation and modern mass-selective detection have been significantly improved as compared with the...

The economic growth of a nation raises the claim for a healthy eco-friendly environment. Therefore, to avoid any disease and problems due to the adverse effect of chiral pollutants, it is essential to monitor the chiral pollutants in our environment. In spite of the advancement of science and technology, data on the chirality of the different pollu...

Enantioselective separations by capillary electrophoresis (CE) have rapidly attracted attention in the early 1980s as a promising field of pharmaceutical sciences after the innovative work of Jorgenson (1988) and by the group of Terabe (Nishi et al. 1990a, b). In 1987, CE instruments became commercially available, largely stimulated by pharmaceutic...

Capillary gas chromatography (cG) has been an important separation technique for enantioselective chromatography from the early beginning of this research field in analytical chemistry. A number of early reports illustrate the importance of this separation technology for enantiomer separation. The relevance of enantioselective high resolution gas c...

Recent success in studying the proteome, as a source of biomarkers, has completely changed our understanding of leukemia (blood cancer). The identification of differentially expressed proteins, such as relapse and drug resistance proteins involved in leukemia by using various ionization sources and mass analyzers of mass spectrometry techniques, ha...

Hydroxychloroquine is a chloroquine derivative which is well recognized for its ‘SARS-CoV-2 or COVID-19’ & other uses. It’s one of the key drugs of malaria and other various respiratory diseases. The drug exhibits a wide range of activities that include strong antimalarial, antidibetic, anticancer, anti-HIV, antifungal, antimicrobial and antioxidan...

Fluopyram, flupyradifurone, and indaziflam are novel systemic pesticides developed by Bayer. Based on available data, these pesticides appears to have a favorable safety profile in the case of pesticide residue problems. Herein, we report an effective analytical method for the determination of novel pesticides were developed using ultra-high perfor...

Now in its updated second edition, this book describes emerging chiral environmental pollutants and their importance in environmental and human toxicology, as well as enantiomer selective separation and analytical methods. Compared to the first edition from 2001, the book has been completely restructured and a wealth of new material has been added...

Aim Recent developments in Fourier transform infrared (FTIR) spectroscopy instrumentation extend the application of this technique to the field of food research, in particular facilitating studies on edible oils and fats. Materials and methods In this work, an attenuated total reflection-FTIR spectroscopy method was used as an effective analytical...

A novel analytical method, based on high-performance liquid chromatography with a UV (HPLC-UV) detection system for the sensitive detection of a genotoxic impurity (GTI) 5-amino-2-chloropyridine (5A2Cl) in a model active pharmaceutical ingredient (API) tenoxicam (TNX), has been developed and validated. The HPLC-UV method was used for the determinat...

Background Indole-3-acetic acid (IAA) is an important growth hormone for plants obtained by biosynthesis from tryptophan. Aim In this paper was studied the competitive transport of two biologically active compounds, indole-3-acetic acid (IAA) and tryptophan (TRP) through a liquid membrane. Methods The separation of the two compounds was obtained...

In this study, a new type of multifunctional material PANI/NiO/MnO 2 nanocomposite was first time prepared by chemical polymerisation from aniline monomer in the presence of metal oxides (NiO and MnO 2) and an oxidant (ammonium persul-fate) in acidic aqueous solution for the elimination of methyl orange (MO) dye from water. Herein, the PANI/NiO/MnO...

Objective: This paper analyzes the potential outliers in the bioanalytical and clinical part of a bioequivalence study, the effect on bioequivalence decisions whether or not it is appropriate to eliminate them from the statistical evaluation of bioequivalence. Materials and methods: The clinical part was a cross-over, two periods, two sequences...

Capacitively coupled contactless conductivity detection (C⁴D) has emerged as influential to detect analytes that do not have chromogenic or fluorogenic functional group. Since our last review several new capillary electrophoresis (CE) methods coupled with (CE-C⁴D) have been communicated. The aim of this review is to give an update of the almost all...

Three stochastic microsensors based on graphite powder modified with three different oleamides: N-(2-piperidin-1-ylethyl)oleamide, N-(3,4-dihydroxyphenethyl)oleamide and N-(2-morpholinoethyl)oleamide, were designed, characterized, and used to assess DNA damage in cancer by assaying two biomarkers namely 8-nitroguanine and 8-hydroxy-2`-deoxyguanosin...

Three stochastic sensors based on reduced graphene oxide (reduced nanographene oxide, reduced graphene oxide decorated with TiO2 and nanoparticles of Au or Ag) paste modified with a complex of protoporphyrin IX with zinc, are reported for the electroanalysis of 8-nitroguanine and 8-hydroxy-2`-deoxyguanosine in whole blood and urine samples. The hig...

An effective, simple and rapid analytical method using HPLC was developed for the analysis of monosodium glutamate (MSG) in various food samples obtained from local market in Turkey. The determination of MSG was performed by its derivatisation with o‐phthaldialdehyde (OPA). A high‐performance liquid chromatography‐ultraviolet/diode array detection...

A hollow-fiber liquid-phase microextraction (HF-LPME), followed by high-performance liquid chromatography–ultraviolet (HPLC–UV) method for the trace determination of carvedilol (β-blocker) in biological fluids, has been described. The separation was achieved using Inertsil ODS-3 C 18 (250 mm × 4.6 mm, 3 μm) column with a mobile phase composition of...

New Schiff’s base (ligand) and its copper, cobalt, nickel and zinc metal ion complexes were synthesized with a good yield of 86 to 68.9%. and characterized by various analytical techniques. The spectral data confirms the formation of the compounds. The ligand and the metal complexes were screened for anticancer activities with A549 and H1299 lung c...

One of the most common techniques for assessing the intestinal absorption characteristics of drugs is single-pass intestinal perfusion (SPIP) method. Metoprolol tartrate (MT, reference standard) and phenol red (PR, zero permeability marker) are the compounds that are normally used in SPIP studies. The aim of this study was to develop a reverse phas...

Adulteration of olive oil with the other cheap oils and fats plays an important role in economics and has nutritional benefits. In this work, metabolite profiling was performed using gas chromatography–mass spectrometry to identify and quantify animal fat (lard) adulteration in vegetable oil (olive oil). Principal component analysis could correctly...

High-performance liquid chromatography (HPLC) and solid phase micro membrane tip extraction (SPMMTE) methods are developed for the simultaneous analysis of eleven cardiovascular drugs in human plasma. Iron nanoparticles were obtained by the green method, characterized by XRD, FT-IR, TEM, and EDS and utilized in SPMMTE for sample preparation. The mo...

Triadimenol is the most important triazole fungicide used for prevention and treatment of plant fungal disease. A simple high performance liquid chromatography (HPLC) method for triadimenol analysis in water sample is reported in this study. The optimized HPLC system using C 18 column was achieved using mobile phase composition containing acetonitr...

Aim A new, accurate and sensitive reversed-phase high-performance liquid chromatography (RP-HPLC) as an analytical method for the quantitative determination of eight antifungal drugs in spiked human plasma has been described optimized and validated. Methods and Materials The analyzed compounds were voriconazole (VOR), luliconazole (LUL), clotrimaz...

Background Acne is caused by several factors including the active secretion of sebaceous sweat glands, hyperkeratosis in the hair infundibulum and the effects of bacteria. One of the plants that has the potential as an antibacterial is the extract of arumanis mango leaves. Method Determine the Minimum Inhibitory Concentration (MIC) of methanol ext...

Background: The total antioxidant capacity of YAM food grown in southern African regions was investigated by poly-glycine glassy carbon modified electrode according. Modified electrode was fabricated using glycine solution by electro-deposition method on glassy carbon electrode. The proposed modified electrode is nearly 3-fold more sensitive than t...

Background: A series of eight new flavone derivatives containing a piperazine chain with different substitution were synthesized and their structures were determined. Methods: Their antiradical and antioxidant activities were evaluated using superoxide anion radical, hydroxyl radical, 2,2-diphenyl-1-picrylhydrazyl radical, 2,2'-azino-di(3-ethylb...

Background Stilbene phytalexis (1,2-diphenyloethylen) and benzamide are beneficial for human health. To increase the stilbene ring activity, a new series of its derivatives containing benzamide structure was synthesized and evaluated for their in vitro antioxidant power. Methods 1H nuclear magnetic resonance, mass spectroscopy, and chromatographic...

Aims A Fourier Transform Infrared (FT-IR) spectrometric method was developed for the rapid, direct measurement of Raspberry Ketone (RK) and Caffeine (CAF) in a nutraceutical formulation. Methods Conventional KBr-spectra and KBr+0.5 mg Microcrystalline Cellulose (MCC)-spectra were used as the basis for a better determination of active substances in...

An investigation was reported concerning the chiral analysis of some Imino-4-Hesperetin and the impact of different factors on their chiral high-performance liquid chromatographic separation. Using Schiff bases reactions, new Imino-4-Hesperetin derivatives had been elaborated by the reaction of Hesperetin with various diamines differ only in their...

There is increasing need to address the potential risks of combined exposures to multiple residues from pesticides in the diet. The pesticide residues pollution is one of the problems that arise due to the increased uses of pesticides to meet the higher demands of food production for human consumption. In this study, pesticide residue data were ach...

An investigation was reported concerning the chiral analysis of some Imino-4-Hesperetin and the impact of different factors on their chiral high-performance liquid chromatographic separation. Using Schiff bases reactions, new Imino-4-Hesperetin derivatives had been elaborated by the reaction of Hesperetin with various diamines differ only in their...

Chiral stationary phases are conveniently used for enantiomeric separation of drugs by liquid chromatography. Consumption of large volumes of hazardous solvents is considered as a common challenge for the sustainability of this technique. To this end, a columnar chromatography has been adopted using 50‐mm‐length stationary phases. The study compris...

DOI: 10.1002/sscp.201900021 The cover picture shows the chromatograms of ESC‐OX and its impurities by using; (a) Nucleosil® 100 C18 (3 µm, 10 × 0.46 cm), (b) Synergi Hydro‐RP® (4 µm, 15 × 0.46 cm), (c) Fluophase RP® (3 µm, 10 cm × 4.6 mm), (d) Ascentis® Express C18 (2.7 µm, 10 cm × 4.6 mm), (e) Ascentis® Express F5 (2.7 µm, 10 cm × 4.6 mm), (f) Asc...

It is well known that the chiral separation is different but very important in separation science. Increase in number of chiral centers makes the chiral separations more difficult. In this respect multidimensional chromatography is gaining importance in chiral separations. There is a great demand of multidimensional chromatography in the chiral sep...

The commercial value of Cashew nut shell liquid (CNSL) has become a corner stone in agro waste industry as it is the by‐product of the cashew industry which has 1/8 inch thickness of soft honey comb structure. CNSL contains phenolic lipids with aliphatic chains such as anacardic acid, cardanol, cardol, and methyl cardol, and their derivatives. The...

The liquid chromatographic separation performance of 12 different columns was evaluated for the estimation of the escitalopram oxalate and six of its impurities in different pharmaceutical formulations. All columns exhibited a reasonable separation power. Six columns with three different chemistries and two different particle morphology (i.e. core‐...

Currently, there is growing interest in the degradation pathways of organic contaminants such as pesticides. In the case of pesticides, the determination of metabolites in agricultural products and environment is necessary as some of them could present similar toxicity to or even higher toxicity than the parent compound. The development of analytic...

The body of evidence from the past three decades demonstrates that oxidative stress can be involved in several diseases. This study aims to summarise the current state of knowledge on the association between oxidative stress and the pathogenesis of some characteristic to the biological systems diseases and aging process. This review also presents t...

A simple, fast and highly sensitive RP-UPLC-MS/MS method was developed and validated for the simultaneous determination of sofosbuvir (SR) and its metabolite GS331007 in human plasma using ketotifen as an internal standard (IS). The separation was achieved on Acquity UPLC BEH C 18 (50 × 2.1 mm, i.d. 1.7 µm, Waters, USA) column using acetonitrile:5...

In this study, the concentrations of lead, cadmium and manganese were determined in horse mackerel (Trachurus mediterraneus) and whiting (Merlangius merlangus euxinus) that were caught in the Sea of Marmara. These are commonly consumed fish species in this region. Fish were provided by a fishermen quarterly between March 2013 and December 2013 and,...

A quality-by-design approach was adopted for enantioseparation of atenolol on Vancomycin and Teicoplanin-based chiral stationary phases using reversed phase (RP) mode and polar ionic mode (PIM), respectively to account for major forces involved in enantiorecognition of β-blockers on macrocyclics. A fractional factorial screening design for the two...

A simple and fast capillary zone electrophoresis method with direct ultraviolet detection has been developed for the quantitative analysis of veterinary drug florfenicol. Migration time of 1.22 minute was achieved using 1 mM borate buffer of pH 9.3 as background electrolyte. Limit of detection and limit of quantification for this method are 3.34 μg...

Inductively coupled plasma is a new technique employed for the determination of elemental impurities in pharmaceutical ingredients viz. raw materials, drug substance, and drug product dosage forms. New US FDA and EMA regulations came into effect from Jan 2018 as industry standard requirements for the determination of inorganic elemental impurities...

Aim A new simple and sensitive high-Performance Liquid Chromatography (HPLC) method for the determination of a potent synthetic cannabinoid THJ-2201, has been developed and validated. Lixiviptan was used as the Internal Standard (IS). Methods THJ-2201 and IS were extracted from mouse plasma using deproteinization procedure that uses acetonitrile f...

Objective: Ten racemic 3,4-dihydropyrimidin-2(1H)-ones and 3,4-dihydropyrimidin-2(1H)-thiones were separated by liquid chromatography on Chiralcel®OD-H column containing cellulose tris(3, 5-dimethylphenylcarbamate). Method: The enantioseparation was carried out using of Chiralcel®OD-H polysaccharide-type chiral stationary phase to resolve such enan...

An easily performed, specific, sensitive, rapid, reliable and inexpensive procedure for the spectrofluorometric quantitation of ascorbic acid was proposed using acriflavine as a fluorescence quenching reagent. The procedure was based on the determined quenching effect of ascorbic acid on the natural fluorescence signal of acriflavine and the reacti...

Background Chiral separation involves many phenomena in which the elution order of the enantiomers has its unique position. The phenomenon of elution order of the enantiomers has also been used in the determination of optical purity which is favorable to elute the major component after minor enantiomeric impurity but the main problem is that, this...

The present work describes a capillary electrophoretic (CE) generic strategy used for chiral enantioseparation of Fluconazole (as an example of acidic drugs) and donepezil (as an example of basic drugs). Several modified cyclodextrins (CDs) were applied for enantioseparation of racemates such as highly sulfated α, γ CDs, hydroxyl propyl-β-CD, and s...

The persistent pollutant azo dye, Acid Orange 10, was removed from the environment by an Amberlite IRA 400 anion-exchange resin and then biodegraded by two bacterial strains. Moreover, the pollutant ecotoxicity was studied by a bacterial model. The objective of this study was to offer a new synergetic/compatible physico-chemical and biological meth...

Immunoassay technique performs a fast, simple, reliable, and sensitive analysis of different compounds, being applied in several areas of interest such as clinical analysis for medical diagnosis, as well as in environmental analysis, and food quality control. The latest research activities in this field are represented by the attempts to achieve a...

Background: Chitosan is a natural polymer which has limited solubility. Chitosan is capable of being modified using multiple alternatives for chitosan chemical modifications that provide different desirable properties. Method: N-methyl chitosan was successfully prepared and characterized. N-methyl chitosan was characterized by the FTIR, XRD, SEM, T...

Ioxynil (IO) is iodocyanophenolic type herbicide used in raw crop protection of garlic (Allium sativum L.) and linked with suppression of thyroid hormones and induction of various tumors in mammals by inhibiting of intercellular communications related with connexin43 assembled gap junction channels [1]. Residual amounts of IO as commonly consumed w...

Around us, we have many chiral macromolecules such as protein, carbohydrate, nucleic acid, steroids, peptides and phospholipids. Hence, these compounds exist in enantiomeric forms and we know that only one of the enantiomeric forms is important to us and another may or may not be useful. Therefore, chiral analysis of macromolecules has taken a uniq...

The aim of this research is to produce spherical and porous silica particles from rice husk for chromatographic applications in HPLC columns. After complete combustion of rice husk, white powder of silica was obtained which was dissolved in NaOH and subsequently heated to produce sodium silicate solution. Spherical porous silica gel was synthesized...

Aim: Synthesis of novel 2(2-hydroxyphenyl) pyrimidine/benzothiazole piperazinyl-substituted flavones end evaluate their antioxidant activity. Results: Six novel 2-(2-hydroxyphenyl) pyrimidine/benzothiazole-substituted flavones were synthesized, their structures were confirmed by elementary and spectral analyses. The compounds were evaluated for...

A fast, sensitive, and reliable method for the determination of fipronil and its metabolites (fipronil-sulfone, fipronil sulfide, and fipronil-desulfynil) in eggs was developed by a modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) pretreatment method and ultra-performance liquid chromatography-quadrupole-linear ion trap mass spec...

The present work focuses on reviewing the scientific literature on various types of tungsten-based glasses such as tungsten-phosphate, tungsten-tellurite, tungsten-borate glasses, tungsten-germinate, tungsten-antimonate, and non-traditional tungsten oxide-containing glasses. The glass formation, structure, thermal, some chemical, physical and chara...

In this work, a new, sensitive and accurate high‐performance liquid chromatographic method for the simultaneous determination of acyclovir and co‐administered vitamin B3 and gabapentin used in treatment of herpes zoster disease was developed and validated. Protein precipitation with methanol was performed to extract the cited drugs from plasma. The...

The enantioseparation of three fluoroquinoline antibacterial drugs, namely, flumequine, ofloxacin and lomefloxacin using high-performance liquid chromatography was optimized on seven polysaccharide-derived chiral stationary phases, namely, Chiralpak® IB, chiralpak® IA, Chiralpak® AD, Chiralcel® OJ, Chiralcel® OD, Chiralcel® OD-H and Chiralcel® OZ-3...

Fluoroquinolones are popular class of antibiotics with distinct chemical functionality. Most of them are ampholytes with one chiral center. Stereogeneic center is located either in the side ring of Gatifloxacin (GFLX) or in the quinolone core of Ofloxacin (OFLX). These two amphoteric fluoroquinolones have terminal amino groups in common. The unusua...