Harish Bharambe

Moffitt Cancer Center · Department of Tumor Biology

Adenoid cystic carcinoma (AdCC) is a slow-growing salivary gland malignancy that relapses frequently. AdCCs of the submandibular gland exhibit unique differences in prognosis and treatment response to adjuvant radiotherapy compared to other sites, yet the role of tumor anatomic subsite on gene expression and tumor immune microenvironment (TIME) com...

DDX3X, a DEAD box-containing RNA helicase, is known to play diverse roles in RNA metabolism, stress response, innate immunity, and cancer. Medulloblastoma is the single most common malignant brain tumor in children. DDX3X is recurrently mutated in the WNT and SHH subgroups of medulloblastoma. CRISPR-Cas9 mediated DDX3X knockout was successful in th...

Medulloblastoma, the most common malignant brain tumor in children, consists of four molecular subgroups WNT, SHH, Group 3, and Group 4. Group 3 has the worst survival rate among the four subgroups and is characterized by the expression of retina-specific genes. CRX, the master regulator of the photoreceptor differentiation, is aberrantly expressed...

Medulloblastoma, a common pediatric malignant brain tumor, consists of four distinct molecular subgroups WNT, SHH, Group 3, and Group 4. Exome sequencing of 11 WNT subgroup medulloblastomas from an Indian cohort identified mutations in several chromatin modifier genes, including genes of the mammalian SWI/SNF complex. The genome of WNT subgroup tum...

Medulloblastoma, a highly malignant pediatric brain tumor, consists of four distinct molecular subgroups called WNT, SHH, Group 3, and Group 4 that differ in their clinical characteristics with the WNT subgroup having excellent survival rate. About 1/3rd medulloblastomas have metastasis at the time of diagnosis suggesting, high invasion potential o...

Medulloblastoma, a highly malignant pediatric brain tumor, consists of four molecular subgroups, namely WNT, SHH, Group 3, and Group 4. The expression of miR-193a, a WNT subgroup-specific microRNA, was found to be induced by MYC, an oncogenic target of the canonical WNT signaling. MiR-193a is not expressed in Group 3 medulloblastomas, despite MYC e...

Genome-wide expression profiling studies have identified four core molecular subgroups of medulloblastoma: WNT, SHH, Group 3 and Group 4. Molecular markers are necessary for accurate risk stratification in the non-WNT subgroups due to the underlying heterogeneity in genetic alterations and overall survival. MiR-204 expression was evaluated in molec...

Over 250 medulloblastoma tumors tissues from an Indian cohort have been molecularly classified using the microRNA based real time RT-PCR assay and correlated with clinical characteristics. The Indian cohort continues to show three distinct features viz. higher incidence of the WNT tumors particularly in the adult patients, higher male:female (9:1)...

We report on damage to DNA in an aqueous medium induced by ultrashort pulses of intense laser light of 800 nm wavelength. Focusing of such pulses, using lenses of various focal lengths, induces plasma formation within the aqueous medium. Such plasma can have a spatial extent that is far in excess of the Rayleigh range. In the case of water, the res...

We probe femtosecond laser induced damage to aqueous DNA, relying on strong-field interaction with water wherein electrons and free radicals are generated in situ; these, in turn, interact with DNA plasmids under physiological conditions, producing nicks. Exposure to intense femtosecond pulses of 1350 and 2200 nm light induces single strand breaks...